The invention provides a primer group, a system, a kit and a method for detecting aphelenchoides besseyi. The primer group comprises at least one of a first crRNA, a second crRNA, a third crRNA, a fourth crRNA, a fifth crRNA, a sixth crRNA, a seventh crRNA and an eighth crRNA. The embodiment of the invention provides the primer group, the system, the kit and the method for detecting the aphelenchoides besseyi, and the primer group can accurately and quickly detect the aphelenchoides besseyi and has high sensitivity and specificity. According to the method, a sample with the minimum concentration of 1 copy / mu L can be efficiently detected, and the fluorescence value can be increased by increasing the reaction time, so that the accuracy of the detection result is improved, and the detection of the amount of the aphelenchoides besseyi at the early stage can be realized, thereby preventing the aphelenchoides besseyi disease.