46 results about "Aphelenchoides besseyi" patented technology

The invention discloses a rice seed treating agent containing prothioconazole, imazalil and cartap and application thereof. The rice seed treating agent comprises effective components including prothioconazole, imazalil and cartap, wherein a mass ratio of prothioconazole with bactericidal activity to imazalil with bactericidal activity to cartap with insecticidal activity is (1-15): (1-15): (1-15). The invention also discloses application of the seed treating agent in prevention and treatment of rice bakanae disease or / and rice seedling rot or / and rice aphelenchoides besseyi disease. The seedtreating agent disclosed by the invention can be used for effectively treating the drug resistance of Fusarium fujikuroi to bactericides such as carbendazim, prochloraz and JS399-19, and meanwhile, the potential drug resistance risk of Fusarium fujikuroi to prothioconazole and imazalil is reduced. After prothioconazole, imazalil and cartap are combined, a brand-new new product is developed; and the obtained rice seed treating agent has remarkable the effect on preventing and treating rice bakanae disease, rice seedling rot and rice aphelenchoides besseyi disease, is obvious in synergistic effect, can reduce the use amount of pesticides, and is environmentally friendly and safe.

The invention discloses a compound pesticide containing fosthiazate, tebuconazole and prochloraz, a preparation method and an application thereof, wherein the compound pesticide includes 1-15 parts of fosthiazate, 0.05 to 10 parts of tebuconazole and 0.5 to 2 parts of prochloraz. The compound pesticide can effectively overcome the disadvantage that the traditional insecticide cartap for preventing and treating aphelenchoides besseyi is narrow, degradable and unsteady, a treating agent for avoiding rice seed to spread disease can be developed into an emulsifiable concentrate, an emulsion in water, a suspension concentrate and a high-efficiency environmental-protection agent. The compound pesticide is compounded with tebuconazole and prochloraz to enhance fosthiazate permeability and obviously reduce fosthiazate dosage in per mu.

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention discloses a strain TX-4 used for preventing diseases of aphelenchoides besseyi or vegetable root knot nematode and a preparation thereof, belonging to the technical field of preventing and treating diseases of crops and being specially used for biological prevention of the crops. The prevention result of field demonstration shows that the strain has the preventing effect of more than90 percent to the aphelenchoides besseyi disease and has preventing effect of more than 70 percent to the vegetable root knot nematode disease. The strain TX-4 for preventing diseases of aphelenchoides besseyi or vegetable root knot nematode is bacillus with the Latin systematic name of Paenibacillus spp., the strain has been preserved in China Center for Type Culture Collection on March 19, 2009and the preserving number is CCTCC No: M209038.

The invention discloses molecular markers linked with an Aphelenchoides besseyi Christie resistant QTL (quantitative trait locus). The essence of the molecular markers is that according to the linkage and segregation rules, the population of the hybrid generation F2 of the Aphelenchoides besseyi Christie resistant variety Tetep and the susceptible variety Huaidao 5 is taken as the mapping population, and the SSR (simple sequence repeat) markers RM5626 and RM7097, linked with the Aphelenchoides besseyi Christie resistant QTL, on the chromosome 3 are obtained through analysis of linkage between the identification results of Aphelenchoides besseyi Christie resistance and the SSR marker data. Whether a plant has Aphelenchoides besseyi Christie resistance can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the Aphelenchoides besseyi Christie susceptible variety. After being applied to assistant selection breeding of the Tetep and derived varieties (lines) thereof / susceptible variety-Aphelenchoides besseyi Christie resistant variety, the SSR markers can overcome the defects of poor stability and repeatability of Aphelenchoides besseyi Christie resistance identification, time and labor waste and the like in conventional breeding and can simplify the selection method and improve the breeding efficiency so as to accelerate the breeding process of the Aphelenchoides besseyi Christie resistant variety.

The invention discloses a primer for detecting aphelenchoides besseyi in rice seeds and seedlings and application thereof. Alkaline base sequences of the primer are shown in SEQ ID NO:1 and SEQ ID NO:2. The primer has the advantages that the primer can be used for detecting the aphelenchoides besseyi in the rice seeds and seedlings, and a kit for detecting the aphelenchoides besseyi in the rice seeds and seedlings can be prepared; compared with the prior art, the detection speed is quick, and the specificity is good; the popularization and application value is realized.