46 results about "Aphelenchoides besseyi" patented technology

The invention discloses a method for detecting aphelenchoides besseyi, and the method comprises the following steps: using SEQ ID NO: 1 and SEQ ID NO: 2 in a sequence table as a primer pair to carry out polymerase chain reaction; determining whether a specific fragment is obtained or not by electrophoretic analysis; and then detecting or identifying whether the specific fragment is the aphelenchoides besseyi or not. The invention also provides a reagent kit for diagnosing the aphelenchoides besseyi disease, which contains the primer pair. The method and the reagent kit have rather high reliability and sensitivity, the operation is convenient and does not rely on experience of an operator, the accuracy is high, the practicability is strong, and the method and the reagent kit can meet the needs of fast and reliable detection and identification of the aphelenchoides besseyi before agricultural crops are planted and in the plant quarantine process.

The invention relates to a culturing method for a fungus having plant nematode poisoning activity and a preparation method of a metabolite of the fungus, belonging to the technical field of microbial pesticides. A fungus strain Snef5 related to the invention is gloeotinia nematicida which is collected in the China General Microbiological Culture Collection Center (Address: Datun Road, Chaoyang District, Beijing, Post Code: 100101) with the collection number CGMCC No.4549 on January 24, 2011. The taxonomic statuses of the fungus strain Snef5 are ascomycota, ascomycetes, helotiales and sclerotiniaceae, and gloeotinia belongs to a fungus. The fungus is prepared by performing liquid fermentation culturing. A metabolite of the fungus has very high toxicity on major pathogen nematodes in plant nematodes such as root-knot nematodes, soybean cyst nematodes and aphelenchoides besseyi, a remarkable nematode killing effect and good application and development prospects.

The invention discloses a nematicidal composition containing fosthiazate. The nematicidal composition contains active ingredients, i.e. an oleander extract and the fosthiazate, wherein the oleander extract is extracted from oleander leaves or barks; the oleander extract and the fosthiazate are mixed to have a synergistic effect on soybean cyst nematode and aphelenchoides besseyi Christie in a certain proportional region; the mass fraction proportion of the oleander extract to the fosthiazate is (50 to 1) to (1 to 20), preferably (30 to 1) to (1 to 5), further preferably (9 to 1) to (5 to 1), and most preferably (9 to 1 and (5 to 1). The nematicidal composition can be also processed into a pesticide preparation comprising accessory ingredients and active ingredients, wherein the mass fraction percentage content of the oleander extract and the fosthiazate in the composition is 1 percent to 95 percent, and the balance is the pesticide accessory ingredients. The nematicidal composition issuitable for preventing and controlling crop nematodosis such as chlorosis caused by the soybean cyst nematode and plant nematodosis such as aphelenchoides besseyi Christie caused by the aphelenchoides besseyi Christie.

The invention discloses molecular markers linked with Aphelenchoides besseyi Christie resistant QTL (quantitative trait loci). The essence of the molecular markers is that according to the linkage and segregation rules, the population of the hybrid generation F2 of the Aphelenchoides besseyi Christie resistant variety Tetep and the susceptible variety Huaidao 5 is taken as the mapping population, and the SSR (simple sequence repeat) markers RM163, RM18620, RM440 and RM161, linked with two LRGW (loss rate of panicle weight) related Aphelenchoides besseyi Christie resistant QTL, on the chromosome 5 are obtained through analysis of linkage between the identification results of Aphelenchoides besseyi Christie resistance and the SSR marker data. Whether a plant has Aphelenchoides besseyi Christie resistance can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the Aphelenchoides besseyi Christie susceptible variety. After being applied to assistant selection breeding of the Tetep and derived varieties (lines) thereof / susceptible variety-Aphelenchoides besseyi Christie resistant variety, the SSR markers can overcome the defects of poor stability and repeatability of Aphelenchoides besseyi Christie resistance identification, time and labor waste and the like in conventional breeding and can simplify the selection method and improve the breeding efficiency so as to accelerate the breeding process of the Aphelenchoides besseyi Christie resistant variety.

The invention discloses a rice seed treating agent containing prothioconazole, imazalil and cartap and application thereof. The rice seed treating agent comprises effective components including prothioconazole, imazalil and cartap, wherein a mass ratio of prothioconazole with bactericidal activity to imazalil with bactericidal activity to cartap with insecticidal activity is (1-15): (1-15): (1-15). The invention also discloses application of the seed treating agent in prevention and treatment of rice bakanae disease or / and rice seedling rot or / and rice aphelenchoides besseyi disease. The seedtreating agent disclosed by the invention can be used for effectively treating the drug resistance of Fusarium fujikuroi to bactericides such as carbendazim, prochloraz and JS399-19, and meanwhile, the potential drug resistance risk of Fusarium fujikuroi to prothioconazole and imazalil is reduced. After prothioconazole, imazalil and cartap are combined, a brand-new new product is developed; and the obtained rice seed treating agent has remarkable the effect on preventing and treating rice bakanae disease, rice seedling rot and rice aphelenchoides besseyi disease, is obvious in synergistic effect, can reduce the use amount of pesticides, and is environmentally friendly and safe.

The invention discloses a compound pesticide containing fosthiazate, tebuconazole and prochloraz, a preparation method and an application thereof, wherein the compound pesticide includes 1-15 parts of fosthiazate, 0.05 to 10 parts of tebuconazole and 0.5 to 2 parts of prochloraz. The compound pesticide can effectively overcome the disadvantage that the traditional insecticide cartap for preventing and treating aphelenchoides besseyi is narrow, degradable and unsteady, a treating agent for avoiding rice seed to spread disease can be developed into an emulsifiable concentrate, an emulsion in water, a suspension concentrate and a high-efficiency environmental-protection agent. The compound pesticide is compounded with tebuconazole and prochloraz to enhance fosthiazate permeability and obviously reduce fosthiazate dosage in per mu.

The invention relates to an ATP synthetase gene for prevention and control of aphelenchoides besseyi christie, and primers and an application thereof. The gene has the sequence shown in a sequence table SEQ No.1. A primer group for construction of the aphelenchoides besseyi christie ATP synthase gene comprises an upstream primer Ab-ATPs-F shown in the sequence table SEQ No.2 and a downstream primer Ab-ATPs-R shown in the sequence table SEQ No.3. The application of the gene in prevention and control of the aphelenchoides besseyi christie is provided. The ATP synthetase gene has the expression up-regulated after the aphelenchoides besseyi christie is subjected to hypoxia stress, so the gene is indicated to play a role in the hypoxia stress resisting process of the aphelenchoides besseyi christie. Through the study of the gene, the gene has important biological significance and potential application value in the prevention and control of the aphelenchoides besseyi christie.

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention provides a primer group, a system, a kit and a method for detecting aphelenchoides besseyi. The primer group comprises at least one of a first crRNA, a second crRNA, a third crRNA, a fourth crRNA, a fifth crRNA, a sixth crRNA, a seventh crRNA and an eighth crRNA. The embodiment of the invention provides the primer group, the system, the kit and the method for detecting the aphelenchoides besseyi, and the primer group can accurately and quickly detect the aphelenchoides besseyi and has high sensitivity and specificity. According to the method, a sample with the minimum concentration of 1 copy / mu L can be efficiently detected, and the fluorescence value can be increased by increasing the reaction time, so that the accuracy of the detection result is improved, and the detection of the amount of the aphelenchoides besseyi at the early stage can be realized, thereby preventing the aphelenchoides besseyi disease.

The invention discloses molecular markers linked with an Aphelenchoides besseyi Christie resistant QTL (quantitative trait locus). The essence of the molecular markers is that according to the linkage and segregation rules, the population of the hybrid generation F2 of the Aphelenchoides besseyi Christie resistant variety Tetep and Huaidao 5 is taken as the mapping population, and the SSR (simple sequence repeat) markers RM5526 and RM3912, linked with the LRGW (loss rate of panicle weight) related Aphelenchoides besseyi Christie resistant QTL, on the chromosome 9 are obtained through analysis of linkage between the identification results of Aphelenchoides besseyi Christie resistance and the SSR marker data. Whether a plant has Aphelenchoides besseyi Christie resistance can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the Aphelenchoides besseyi Christie susceptible variety. After being applied to assistant selection breeding of the Tetep and derived varieties (lines) thereof / susceptible variety-Aphelenchoides besseyi Christie resistant variety, the SSR markers can overcome the defects of poor stability and repeatability of Aphelenchoides besseyi Christie resistance identification, time and labor waste and the like in conventional breeding and can simplify the selection method and improve the breeding efficiency so as to accelerate the breeding process of the Aphelenchoides besseyi Christie resistant variety.

The invention discloses a strain TX-4 used for preventing diseases of aphelenchoides besseyi or vegetable root knot nematode and a preparation thereof, belonging to the technical field of preventing and treating diseases of crops and being specially used for biological prevention of the crops. The prevention result of field demonstration shows that the strain has the preventing effect of more than90 percent to the aphelenchoides besseyi disease and has preventing effect of more than 70 percent to the vegetable root knot nematode disease. The strain TX-4 for preventing diseases of aphelenchoides besseyi or vegetable root knot nematode is bacillus with the Latin systematic name of Paenibacillus spp., the strain has been preserved in China Center for Type Culture Collection on March 19, 2009and the preserving number is CCTCC No: M209038.

The invention discloses molecular markers linked with an Aphelenchoides besseyi Christie resistant QTL (quantitative trait locus). The essence of the molecular markers is that according to the linkage and segregation rules, the population of the hybrid generation F2 of the Aphelenchoides besseyi Christie resistant variety Tetep and the susceptible variety Huaidao 5 is taken as the mapping population, and the SSR (simple sequence repeat) markers RM5626 and RM7097, linked with the Aphelenchoides besseyi Christie resistant QTL, on the chromosome 3 are obtained through analysis of linkage between the identification results of Aphelenchoides besseyi Christie resistance and the SSR marker data. Whether a plant has Aphelenchoides besseyi Christie resistance can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the Aphelenchoides besseyi Christie susceptible variety. After being applied to assistant selection breeding of the Tetep and derived varieties (lines) thereof / susceptible variety-Aphelenchoides besseyi Christie resistant variety, the SSR markers can overcome the defects of poor stability and repeatability of Aphelenchoides besseyi Christie resistance identification, time and labor waste and the like in conventional breeding and can simplify the selection method and improve the breeding efficiency so as to accelerate the breeding process of the Aphelenchoides besseyi Christie resistant variety.

The invention discloses a primer for detecting aphelenchoides besseyi in rice seeds and seedlings and application thereof. Alkaline base sequences of the primer are shown in SEQ ID NO:1 and SEQ ID NO:2. The primer has the advantages that the primer can be used for detecting the aphelenchoides besseyi in the rice seeds and seedlings, and a kit for detecting the aphelenchoides besseyi in the rice seeds and seedlings can be prepared; compared with the prior art, the detection speed is quick, and the specificity is good; the popularization and application value is realized.

The invention relates to application of flavonoid compound theaflavanoside II to prevention and treatment of plant nematode diseases. The structure of the flavonoid compound theaflavanoside II is shown as the formula (1). The flavonoid compound theaflavanoside II represented by a compound (1) has strong poisonous activity on plant nematodes, has very strong inhibition activity on the incubation of south root-knot nematode ova, and has very strong poisonous activity on second-stage juveniles of south root-knot nematodes, all-age-period nematodes of pratylenchuspratensi, and all-age-period nematodes of aphelenchoides besseyi. The flavonoid compound theaflavanoside II is extracted from natural plants and is easy to degrade in the environment; compared with a chemical nematicide, the flavonoid compound theaflavanoside II has the properties of low toxin and low residues, and has relatively good compatibility with the environment; and the requirements on green plant protection, sustainable agricultural production and development are met. The formula (1) is shown in the description.