Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof
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A technique for killing nematodes and fungi, which is applied in the field of fungi for killing plant parasitic nematodes and its preparation, and can solve problems such as in-depth research
Inactive Publication Date: 2008-05-21
SHENYANG AGRI UNIV
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[0004] In summary, the use of Trichoderma harzianum to biologically control plant-parasitic nematodes has great theoretical re...
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Embodiment 1
[0019] Embodiment 1: the cultivation of Trichoderma bacterial strain Snef85
[0020] The mycelium of Trichoderma strain Snef85 was inoculated on test tube agar medium, and the medium formula was PDA medium, that is, potato 200g; glucose 20g; agar 17g; distilled water 1000mL. Incubate at 25°C for 10 days to obtain test tube seeds.
[0021] Then inoculate the test tube into a 100mL Erlenmeyer flask (50mL per bottle) liquid medium (Chape's medium), the medium formula is: NaNO 3 : 2.0g, K 2 HPO 4 : 1.0g, KCl: 0.5g, MgSO 4 ·7H 2 O: 0.5g, FeSO 4 : 0.01g, sucrose: 30.0g; distilled water: 1000mL. At 25°C to 28°C, the shaking table rotates at 150r·min -1 ~200r·min- 1 , fermentation 168h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.
Embodiment 2
[0022] Example 2: Nematicidal activity of Trichoderma strain Snef85:
[0023] On the basis of concentrating the 10× solution, the fermentation broth was successively diluted into 6 concentration gradients of 1× (stock solution), 5×, 8×, 10×, 15×, and 20× solution to treat root-knot nematodes (Meloidogyne spp.), Rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines).
[0024]1. Preparation of preparations for testing
[0025] For the Trichoderma strain Snef85 prepared by the aforementioned liquid fermentation culture method, the nematicidal activity test was carried out with different multiples of the fermentation broth.
[0026] 2. Preparation of Nematodes for Experiments
[0027] Root-knot nematode (Meloidogyne spp.): collected from tomatoes infested by root-knot nematode in the Hunnan area of Shenyang. Take out the tomato root system, rinse it gently with water, carefully remove the oocysts, sterilize it in 1% sodium hypochlori...
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Abstract
The invention relates to a method for cultivating a fungus capable of poisoning and killing plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The Trichoderma strain Snef85 involved in the present invention is Trichoderma harzianum, which has been preserved on October 30, 2007 in the General Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing, postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the Trichoderma strain Snef85 is Deuteromycotina, Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and Trichoderma harzianum. It is prepared by liquid fermentation culture, and its metabolites have high toxicity to plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean cyst nematodes, and outstanding nematicidal effect. The fermentation broth of the strain has obvious control effects on root-knot nematode (Meloidogynes pp.), rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines). There are significant differences between the treatment of fermented liquid with different dilution concentrations and the treatment of aseptic water, and the nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.
Description
Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a fungus for poisoning and killing plant parasitic nematodes, a preparation method and application thereof. Background technique: [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while actual ...
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