47 results about "Meloidogynes" patented technology

The invention discloses a compound microorganism bacterium agent for preventing and treating meloidogyne and a preparation method of the compound microorganism bacterium agent. The compound microorganism bacterium agent comprises the following ingredients: paecilomyceslilacinus (Thom) samson fermentation liquid and verticilliumchlamydosporium fermentation liquid. The field meloidogyne prevention and treatment test results show that after paecilomyceslilacinus (Thom) samson microbial inoculums and verticilliumchlamydosporium microbial inoculums are compounded, the paecilomyceslilacinus (Thom) samson microbial inoculums and the verticilliumchlamydosporium microbial inoculums show the very obvious cooperative synergia meloidogyne prevention and treatment effect through being compared with the single paecilomyceslilacinus (Thom) samson microbial inoculums or the verticilliumchlamydosporium microbial inoculums, and the prevention effect on the meloidogyne is obviously improved. In addition, the integral prevention effect of the compound microorganism bacterium agent on the meloidogyne is also more and more stable.

The invention relates to a loop mediated isothermal amplification (LAMP) detection method for meloidogyne hapla and application of the method and belongs to the field of biological technology. 5 LAMP primers (namely, MHF3, MHB3, MHFIP, MHBIP and MHLB) are designed in the sequence conservative region according to the cloning sequencing meloidogyne hapla ITS sequence; the 5' end of the probe MH-FITC-Probe is marked by FITC; and the 5'end of the MHFIP primer is marked by the biotin. The DNA of the meloidogyne hapla is extracted, isothermal amplification is performed by the mixture of the specific primers, reaction buffer liquor and reaction enzyme, and the amplification product is subjected to color developing detection, so that the meloidogyne hapla can be detected quickly. The detection method is high in specificity, high in sensitivity, low in cost, and simple and convenient to operate, and has a high application value in the aspects of on-site quick detection on the meloidogyne haplaand early diagnosis on the meloidogyne hapla disease.

The invention provides a novel biological broad-spectrum pesticide and a preparation method thereof. The novel biological broad-spectrum pesticide is prepared by mixing and fermenting ginger, hot pepper, pepper, tobacco leaves and soybean milk fermenting liquid according to a certain weight ratio. The novel biological broad-spectrum pesticide adopts natural plants and probiotics as a core, is prepared through pure biological fermentation, is not added with any chemically synthetic ingredients, has the characteristics of broad spectrum, safety, environmental friendliness and the like, can be applied to preventing and controlling insect damage of organic food, green food and agricultural exports, is a new-generation green pesticide, can be widely applied to controlling agricultural and forest pests of vegetables, fruit trees, gardening flowers, tea gardens and fields, has obvious effect of killing various pests, such as Heliothis armigera, aphid, lice, grubs, red spiders, size insects, Meloidogyne and the like, of crops in agriculture and forestry, is particularly applicable to production of green pollution-free agricultural products and agricultural exports, and can meet requirements on detection of any residues.

The invention discloses a method for breeding meloidogynes by utilizing a single allosome of a separated root system of a non-exogenous cultured water spinach, and the method comprises the following steps: (1) preparing the separated root system of the water spinach: seeding the seeds of the water spinach onto a flat plate of a 1% water agar medium, cutting root tips when the seeds grow into rootsystem with the length of about 3-4cm, inoculating the root tips onto the flat plate of a non-exogenous separated root system culturing medium, and growing a large number of separated roots after 15-20 days; and (2) inoculating and breeding the meloidogynes: preparing oocysts of the meloidogynes into an egg grain suspension solution, inoculating the egg grain suspension solution onto the separated roots, obtained in the steps (1), of the water spinach, and culturing for 50-90 days, thereby obtaining a large number of signal meloidogynes. The method provided by the invention is high in breeding rate, simple in operation, difficult in pollution, wide in application scope and strong in practicability; the problem of difficulty in obtaining a large number of purified meloidogynes is solved; and the defects of the method for breeding the meloidogynes by utilizing potting seedling that time is wasted, a large space is occupied, the method is limited by natural and environmental conditions of seasons, the meloidogynes are easily polluted by other organisms, and the like, are avoided.

The invention discloses paecilomyces lilacinus wettable powder, a preparation method and application thereof. The paecilomyces lilacinus wettable powder comprises the following components of paecilomyces lilacinus spore powder or fermentation liquor, a vector, a wetting agent and a dispersing agent, wherein the wetting agent is selected from any one or more of D1002, NNO or sodium lignin sulfonate; and the paecilomyces lilacinus wettable powder also comprises an ultraviolet protection agent or / and a synergist. According to the paecilomyces lilacinus wettable powder, the traditional wetting agents and dispersing agents are screened and optimized to obtain a specific species and a dosage having a smaller influence on the growth of the paecilomyces lilacinus; in addition, the ultraviolet protection agent for preventing paecilomyces lilacinus spores from being damaged by ultraviolet and the synergist for promoting plants to take roots or increasing the meloidogyne killing effect are further screened and obtained. The invention further provides the preparation method of the paecilomyces lilacinus wettable powder and the application of the wettable powder in preventing and controlling plant meloidogyne.

The present invention relates to a fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof, wherein two fungi cultures such as Purpureocillium lilacinum CGMCC No.9344 and Clonostachys rosea CGMCC No.1037 are cultured to prepare the fungi mixing bacterial agent. According to the present invention, the fungi mixing bacterial agent can be mixed with seedling breeding mediums, organic fertilizers and organic-inorganic compound fertilizers or be used separately so as to perform soil treatment or hole application before or after transplantation, or root-irrigation, and is used for preventing and controlling plant meloidogyne diseases.

The invention relates to a preparation for reducing meloidogyne and a preparation method thereof. Each liter of preparation contains 25 to 35 milligrams of trichoderma harzianum spore powder, 2 to 5 grams of maltose, 0.05 to 0.07 gram of ferrous sulfate, 0.6 to 0.8 gram of magnesium sulfate, 0.6 to 0.7 gram of composite amino acid, 0.04 to 0.06 gram of boric acid, 8 milliliters of ethoxy trisiloxane, 1.0 to 2.0 milliliters of Tween-80, and the balance of water. The preparation is easy to prepare, has the advantages of convenience in use and low cost, and does not have side effect. 40 to 60 percent of root knots of a plant can be remarkably reduced; and after the preparation is used, the resistance of the plant can be remarkably improved, and the consumption of pesticide is reduced. The preparation is suitable for crops such as vegetables, fruit trees, flowers and tea leaves, and is suitable to be popularized and applied in vast rural areas in China.

The invention discloses a Meloidogyne enterolobii detection kit based on loop-mediated isothermal amplification and application thereof. The invention provides special primers for assisted identification of Meloidogyne enterolobii, which are composed of DNAs (deoxyribonucleic acids) disclosed as Sequences 1 to 6 in the sequence table. The kit disclosed by the invention contains the special primers. By designing specific primers and optimizing reaction conditions, the invention provides a kit and method for qualitatively detecting Meloidogyne enterolobii 5S rDNA-IGS2 region. The invention solves the problems of high cost, low efficiency, low speed, and incapability of satisfying demands for plant quarantine and plant protection in the conventional molecular detection.

A preparation capable of inducing cucumber to resist meloidogyne is characterized in that the preparation is prepared from a chitosan oligosaccharide solution and sodium salicylate, wherein the weight ratio of the chitosan oligosaccharide solution to the sodium salicylate is 10-100:1-10. The preparation method comprises the following steps of: cultivating fungus capable of producing chitosanase through fermentation by taking chitosan, yeast extract, NH4Cl, glucose, KH2PO4, MgSO4.7H2O, MnSO4 and water as a culture medium; preparing fermentation liquor containing chitosanase; and performing enzymolysis on the chitosan through the fermentation liquor to obtain the chitosan oligosaccharide solution, and mixing the chitosan oligosaccharide solution and the sodium salicylate to prepare the preparation. The preparation provided by the invention can be used for preventing and controlling the cucumber meloidogyne, has a control effect of 50 to 70 percent, can simultaneously promote growth of cucumber root and has a remarkable effect on ecological soil remediation.

The invention discloses a PCR detection reagent of apple meloidogynes and a reagent box. The PCR detection reagent comprises a pair of specific primers; the nucleotide sequence of an upstream primer is GGGACCGACGGCTTAGTG; the nucleotide sequence of a downstream primer is CCGTTACACGACGAGAGTC. The specific fragment of the specific primers to specificity, sensitivity and amplification of the apple meloidogyne is 250 pb; the specific primers have no specific band fragment to other meloidogynes. By adopting the specific primers and the reagent box provided by the invention, test can be completed within four hours, the sensitivity is high, and the practicability is strong.

The invention discloses an eelworm killing metarhizium and the application thereof. The metarhizium is metarhizium anisopliae var.anisopliae HYN-1-2 which is collected in the China general microbiological culture collection center on 31st October, 2011 and the collection number is CGMCC No.5433. The metarhizium and metarhizium preparations are used for controlling plant meloidogyne; the controlling effect can reach 50-70 percent and is relatively stable; the active constituents of the metarhizium and the metarhizium preparations are good in fixed planting effect in soil, non-toxic and pollution-free in use, high in performance price ratio, convenient in application, and wide in application prospect.

The invention discloses paecilomyces lilacinus wettable powder, a preparation method and application thereof. The paecilomyces lilacinus wettable powder comprises the following components of paecilomyces lilacinus spore powder or fermentation liquor, a vector, a wetting agent and a dispersing agent, wherein the wetting agent is selected from any one or more of D1002, NNO or sodium lignin sulfonate; and the paecilomyces lilacinus wettable powder also comprises an ultraviolet protection agent or / and a synergist. According to the paecilomyces lilacinus wettable powder, the traditional wetting agents and dispersing agents are screened and optimized to obtain a specific species and a dosage having a smaller influence on the growth of the paecilomyces lilacinus; in addition, the ultraviolet protection agent for preventing paecilomyces lilacinus spores from being damaged by ultraviolet and the synergist for promoting plants to take roots or increasing the meloidogyne killing effect are further screened and obtained. The invention further provides the preparation method of the paecilomyces lilacinus wettable powder and the application of the wettable powder in preventing and controlling plant meloidogyne.

The invention discloses a method for detecting a single knot of enterolobium cyclocarpum meloidogyne and application thereof. In the invention, universal primers MF (Forward Primer) and MR (Reverse Primer) for amplifying meloidogyne and specific primers FMe (Forward Primer) and RMe (Reverse Primer) for amplifying the enterolobium cyclocarpum meloidogyne are designed; a single root knot DNA containing the meloidogyne is taken as a template for PCR (Polymerase Chain Reaction) amplification reaction; a PCR amplification reaction product is subjected to lipolysaccharide electrophoresis detection,and a detection result is obtained according to the existence / absence and the size of straps; and the sequences of the primers are respectively shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 in a table. The one-step dual PCR detection method disclosed by the invention has the advantages of increased molecular detection speed, increased sensitivity and accuracy, and has a practicalapplication value in the aspect of detecting the enterolobium cyclocarpum meloidogyne.

The invention relates to an organic preparation for disinfecting flower continuous cropping obstacle sick soil and application thereof. The preparation is a composition of dry plant powder of reeds, potato vines, rice straws, wheat straws, bagasse, bean dregs, broad bean straws, corn straws and the like and molasses, wherein the weight percent of the molasses is 0-100%, and the dry plant powder and the molasses are respectively packaged prior to use and then successively used. The application method is as follows: uniformly spreading the dry plant powder or the molasses on a continuous cropping obstacle flower field according to a preset ratio, and ploughing to bury the plant powder or the molasses into a soil plough horizon; uniformly watering according to a ratio of 30-80 tons of water per mu to keep the soil wet, and covering a film on the ground to isolate the soil from air; controlling the soil temperature at 25-50 DEG C, and enabling the oxidation-reduction potential to reach -60 mv or below so as to form an anaerobic strong reducing state; and keeping the anaerobic strong reducing state for 3-4 weeks, and then removing the covering film. The organic preparation provided by the invention can effectively kill Fusarium oxysporum, Meloidogyne and the like in the soil after flower planting, eliminate continuous cropping obstacles and restore soil characteristics.

The present invention relates to a novel nematode killing composition, wherein the active components of the novel nematode killing composition comprise tioxazafen and iprodione, and a weight of tioxazafen to iprodione is 1:30-30:1. According to the present invention, the novel nematode killing composition provides significant synergistic effect on the prevention and control of meloidogyne diseases of a variety of crops within the compounding range of the active components, is suitable for prevention and control of wheat meloidogyne, cyst nematode, citrus meloidogyne, cucumber meloidogyne, tomato meloidogyne, hot pepper meloidogyne, peanut meloidogyne, soybean cyst nematode and other nematodes, and has characteristics of good prevention and control effect, long lasting effect, and easy promotion and application.

The invention discloses a botanical reagent for prevention and treatment of meloidogyne and a preparation and use method thereof. The raw material is the extract liquid of sorghum at the medium term of grouting and ricinus communis plants at the blossoming and fruit bearing stage. The method is characterized by including the steps of: (1) conducting raw material pretreatment; (2) performing juicing in a pulverizer; (3) filtering a solid-liquid mixture; (4) adding a preservative and conducting storage for standby use; and (5) diluting the extract liquid with water, then subjecting the diluted liquid to root-irrigation or spray to crops so as to play a role of prevention when meloidogyne does not attack the crops and play a good role of control when meloidogyne attacks the crops. The botanical reagent produced from sorghum and ricinus communis plants has the characteristics of high efficiency and quick effect in control of meloidogyne. Also the reagent is added with a preservative, so that the prepared reagent can be stored for a long time and is convenient for use at any time. The process involved in the invention is simple and reduces the production cost. Also the botanical reagent provided by the invention is safe to use and pollution-free, and has wide application prospect.

The invention provides a bacillus megatherium strain D45. The bacillus megatherium strain D45 has a preservation number of CGMCC No.15119, and belongs to the technical field of plant nematode biological prevention and control. A fermentation product of the bacillus megatherium strain D45 has excellent nematode killing activity and can be used for preventing and controlling Meloidogyne graminicolaand Heterodera elachista. Proved by verification, after a fermentation broth of the bacillus megatherium strain D45 is diluted by 2 folds and 5 folds, the root knot inhibition rates on Meloidogyne graminicola are 67.3% and 62.6% respectively, and the prevention and control effects are 53.1% and 56.3% respectively. The prevention and treatment effect of the D45 strain fermentation broth 2-fold diluent on Heterodera elachista is 60%.

The invention discloses a star anise extract for killing Meloidogyne spp. and a preparation method thereof, and provides a method for preparing a star anise absolute ethanol extract. The method comprises the following steps of: 1) uniformly mixing star anise and absolute ethanol to obtain a mixture; and 2) performing ultrasonic treatment on the mixture obtained in step 1), and suction-filtering and concentrating to obtain the star anise absolute ethanol extract. Through the experiment, star anise is extracted by using absolute ethanol, the poisoning effect of extracting solution on the Meloidogyne spp. is tested, and the result is that the extract has strong poisoning activity on the Meloidogyne spp.; therefore, the extract lays a foundation for the development and application of Meloidogyne spp. biocontrol preparations in the future. In the method, an active substance for killing the Meloidogyne spp. is extracted by using a natural harmless plant material, so that the pollution caused by chemical pesticides can be reduced; and the extraction method is simple, and can be easily popularized and applied to the control of plant rootknot nematodes.

The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets show significant conservation at the nucleotide level between orthologs from different Meloidogyne species, facilitating genus-wide targeting by RNA interference.

The invention discloses arthrobacter nicotianae and application thereof in preventing and controlling of white gourd meloidogyne. The arthrobacter nicotianae is a strain 08220203, and is collected into Common Microorganism Center of China Committee for Culture Collection of Microorganisms on January 15th, 2018; the collection number is CGMCC No.15217. Experiments prove that the arthrobacter nicotianae 08220203 has better preventing and control effect on the white gourd meloidogyne; the preventing and controlling effect is 55.33% 14 days after the pesticide is applied the last time; on the basis of growth condition of the white gourd, the arthrobacter nicotianae 08220203 has obvious growth promoting effect on the white gourd, has no pesticide injury, and is safe and reliable.

The invention relates to a pesticide composition for preventing and controlling capsicum meloidogyne. Fluopyram and clothianidin are used as active ingredients. The composition of the fluopyram and the clothianidin has a synergetic effect on the capsicum meloidogyne, improves the prevention and control effect, and delays resistance of the meloidogyne.

The invention relates to a method for preparing liquid stevia rebaudiana functional fertilizer, which is characterized by the following steps: stevia rebaudiana stem and root fully adsorb water, and after full water absorption, the weight ratio between the dry stevia rebaudiana stem and root and water is (15-40):(60-85); and the stevia rebaudiana stem and root after full water absorption are placed in a fermenting tank for fermentation for 10 to 30 days under the temperature ranging from 40 DEG C to 50 DEG C, and the obtained fermentation liquid is the liquid stevia rebaudiana functional fertilizer. The fertilizer can effectively restrain the incidence and the spreading of Meloidogyne, as well as the incidence of soil-borne diseases and improve plant quality. By adopting the fertilizer, without adding any chemical residues in soil, insect pests and diseases can be restrained and the soil quality can be improved at the same time through adopting pure organic fertilizer.

The invention belongs to the technical field of pest prevention and control, particularly relates to a composite microbial agent for preventing and controlling Meloidogyne arenria, and further discloses a preparation method of the composite microbial agent. The composite microbial agent for preventing and controlling Meloidogyne arenria, adopting selected composite microbes and plant extract as effective components, facilitates the synergistic effects of Lactobacillus rhamnosus, Propionibacterium freudenreichii and Bacillus amyloliquefaciens and the effects of the enzymatic hydrolysate of water chestnuts and burdocks, all the components coordinate with each other to exert the insecticidal effect, and indoor test and field test of the composite microbial agent prove that the composite microbial agent of the invention has a good killing effect on the Meloidogyne arenria, and does not affect the growth of peanuts at all.

The invention belongs to the technical field of soil conditioners and provides an old ginger land soil conditioner. The soil conditioner is prepared from, by weight, 45-50 parts of charcoal, 1-5 partsof abamectin, 20-25 parts of plant powder, 1-5 parts of fermented seaweed, 5-10 parts of amino acids and 5-10 parts of calcium-silicon-potassium-magnesium fertilizers. The old ginger land soil conditioner is easy in raw material acquisition, simple in preparation, pest and disease damages are controlled according to a biological prevention and treatment and chemical prevention and treatment combined method on the basis of a biological diversity, and a treatment effect is 2-3 times of that of an antibacterial agent. Therefore, by the soil conditioner, soil meloidogyne damages can be effectively reduced, the soil organic matter content is increased, and chemical phosphate fertilizer consumption can be reduced by 30%.

The invention discloses a method for identifying resistance of a plant to meloidogyne. The method comprises the steps of 1, grafting meloidogyne on a plant, and conducting cultivation; 2, after the step 1 finishes, taking plant root tissue, and observing the growth state of meloidogyne in the plant root tissue; 3, after the step 1 finishes, taking the plant root tissue, and calculating the number of meloidogyne in the plant root tissue; judging the resistance of the plant to meloidogyne according to the result. According to the method for identifying the resistance of the plant to meloidogyne, toluidine blue is applied to dyeing of a root-knot paraffin slice, an optimal dyeing time is found, and the growth state of the nematodes and giant cells can be clearly observed; moreover, the dyeing time is fast, and the effect is good. Aiming at the statistics on the number of the nematodes, a grinding method and a screening method are combined, an optimal grinding time is found, and convenience is brought to the statistics on the number of the nematodes. The two methods can combined into application, and the resistance of the plant to the statistics on the number of the nematodes can be expressed from two aspects of the growth state and the nematodes and the number of the nematodes.

This invention refers to the creation of liquid media to grow the fungus Pechonica chlamydosporia designed to produce conidia of the aleuriospore type, also denominated chlamydospores. These media using easily carried out fermentation processes, achieved productions over 106 chlamydospores / ml. The chlamydospores can then be easily extracted using processes of filtration and separation of solid particles in liquid suspensions. These chlamydospores can be mass collected and utilized to formulate products to use in biological control of Meloidogyne spp. by the nemathophagous fungus P. chlranaydosporia, either in small scale trials or at commercial scale.

The invention belongs to the technical field of agricultural microbiology and discloses an application of 4-vinylphenol to preparing nematode insecticide. That the 4-vinylphenol has a function of killing nematode is first proposed in the invention; the 4-vinylphenol has a very good effect of killing and inhibiting south meloidogyne, haemonchus contortus, caenorhabditis elegans and south meloidogyne eggs; the 4-vinylphenol has the advantages of high efficiency, low toxicity, good specificity and good persistence; and a new selection is provided for preparing novel preparations for preventing and treating plant pathogenic nematode and animal pathogenic nematode.

The invention relates to a composite biological preparation and application thereof in elimination of meloidogyne. The active component contained in the composite biological preparation is a nematodecontrol composition, which contains algae polysaccharide, xylanase, cellulase, protease and beta-glucosidase. The composite biological preparation prepared with the composition is soluble in water, can be added into irrigation liquid and seep into the soil at the same time of irrigation, thus achieving the meloidogyne control effect, the composite biological preparation also can be applied to irrigation treatment on soil before plant sowing, and the use method is simple and effective. The composite biological preparation can reach a nematode control rate up to 90% or more, also is non-toxic and harmless to animals and plants, and is low in cost, thus being suitable for mass production and promotion.

The invention belongs to the technical field of deinsectization devices, and discloses a kiwifruit seedling root deinsectization device. The device comprises a deinsectization box, a solution of copper sulphate is injected into the deinsectization box, a mounting plate is arranged on the surface of the solution of copper sulphate in the deinsectization box, multiple through holes are formed in the mounting plate, and wooden mounting pipes are rotationally connected into the through holes; stirring rods are rotationally connected to the lower portions of the left side wall and the right side wall of the deinsectization box, and the stirring rods penetrate through the side walls of the deinsectization box; a transmission mechanism is arranged at the end, located outside the deinsectization box, of each stirring rod, and a helical coil is connected to the end, located inside the deinsectization box, of each stirring rod; the device further comprises a power supply, and the ends, located outside the deinsectization box, of the two stirring rods are connected with the two ends of the power supply respectively. Accordingly, the problem that in the prior art, kiwifruit seedling roots are likely to breed meloidogyne, and then the survival rate of transplanting is low is solved.