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Arthrobacter nicotianae and application thereof in preventing and controlling of white gourd meloidogyne

A technology of Arthrobacter tobacco and wax gourd root, which is applied in the direction of application, nematocide, bacteria, etc., to achieve the effects of fast growth, good application prospects, and growth promotion effect

Inactive Publication Date: 2018-10-19
毕乃亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Arthrobacter to control the root-knot nematode of wax gourd

Method used

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  • Arthrobacter nicotianae and application thereof in preventing and controlling of white gourd meloidogyne
  • Arthrobacter nicotianae and application thereof in preventing and controlling of white gourd meloidogyne
  • Arthrobacter nicotianae and application thereof in preventing and controlling of white gourd meloidogyne

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Isolation and purification of Arthrobacter tobacco (A.nicotianae) 08220203

[0030] Tobacco Arthrobacter (A.nicotianae) 08220203 of the present invention is obtained from sea mud in Laizhou Wetland, Yantai by using dilution plate method and plate streak method to separate and obtain, and the separation method is:

[0031] (1) Isolation of strains

[0032] The samples were taken from the Laizhou Wetland in Yantai. Weigh 1g of sea mud sample into 100mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 Spread the diluted solution on the LB medium plate, and apply three parallels for each gradient. After culturing at 30°C for 2 days, pick microbial colonies of different forms on the LB medium plate to streak on the LB medium plate, and observe regularly Colony growth. Then use plate streaking method to purify the strains and save them by numbering.

[0033] (2) Scre...

Embodiment 2

[0054] 1. Arthrobacter tobacco fermentation process

[0055] The formula of LB liquid medium is: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 6.9-7.1.

[0056] Solid medium is made up of solid material and inorganic salt, and the mass ratio of described solid material and inorganic salt is 98.7: 0.3; Described solid material is counted as in mass ratio: rice husk: corn flour: soybean meal: fish meal=50:25 : 21: 4; the composition and weight ratio of the inorganic salt are: potassium dihydrogen phosphate 42%, magnesium sulfate 8%, ammonium sulfate 4%, light calcium carbonate 46%. Preparation method: adding an appropriate amount of water (10-15 times the quality) to dissolve the inorganic salt to obtain an inorganic salt solution; mixing the inorganic salt solution with the solid material, and adding water to finally adjust the water content of the solid medium to 40-60%, so that the Hold the solid medium in your hand, and it is ad...

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Abstract

The invention discloses arthrobacter nicotianae and application thereof in preventing and controlling of white gourd meloidogyne. The arthrobacter nicotianae is a strain 08220203, and is collected into Common Microorganism Center of China Committee for Culture Collection of Microorganisms on January 15th, 2018; the collection number is CGMCC No.15217. Experiments prove that the arthrobacter nicotianae 08220203 has better preventing and control effect on the white gourd meloidogyne; the preventing and controlling effect is 55.33% 14 days after the pesticide is applied the last time; on the basis of growth condition of the white gourd, the arthrobacter nicotianae 08220203 has obvious growth promoting effect on the white gourd, has no pesticide injury, and is safe and reliable.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. Specifically, the present invention relates to a strain of Arthrobacter tobacco capable of effectively preventing and treating root-knot nematode disease of wax gourd and its use, and a biological control preparation prepared by using the Arthrobacter tobacco. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is a kind of phytopathogenic nematode that is extremely harmful to plants, especially vegetable crops. In cucumber, winter melon, tomato, eggplant, lettuce, carrot, cabbage and leguminous vegetables and other vegetables. So far, there have been more than 80 kinds of root-knot nematodes reported, including southern root-knot nematode (Meloidogyne incognita), Javan root-knot nematode (M.javanica), northern root-knot nematode (M. M. arenaria) is the most important species of plant parasitic nematodes because of its wide distribution and multi-parasit...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/06
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/06
Inventor 毕乃亮牛赡光刘宜鑫张淑静牛兆颖李宗泰
Owner 毕乃亮
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