Method for breeding meloidogynes by utilizing single allosome of separated root system of non-exogenous cultured water spinach

Abstract

The invention discloses a method for breeding meloidogynes by utilizing a single allosome of a separated root system of a non-exogenous cultured water spinach, and the method comprises the following steps: (1) preparing the separated root system of the water spinach: seeding the seeds of the water spinach onto a flat plate of a 1% water agar medium, cutting root tips when the seeds grow into rootsystem with the length of about 3-4cm, inoculating the root tips onto the flat plate of a non-exogenous separated root system culturing medium, and growing a large number of separated roots after 15-20 days; and (2) inoculating and breeding the meloidogynes: preparing oocysts of the meloidogynes into an egg grain suspension solution, inoculating the egg grain suspension solution onto the separated roots, obtained in the steps (1), of the water spinach, and culturing for 50-90 days, thereby obtaining a large number of signal meloidogynes. The method provided by the invention is high in breeding rate, simple in operation, difficult in pollution, wide in application scope and strong in practicability; the problem of difficulty in obtaining a large number of purified meloidogynes is solved; and the defects of the method for breeding the meloidogynes by utilizing potting seedling that time is wasted, a large space is occupied, the method is limited by natural and environmental conditions of seasons, the meloidogynes are easily polluted by other organisms, and the like, are avoided.

Description

technical field [0001] The invention relates to an artificial propagation method of root-knot nematodes, in particular to a method for propagating root-knot nematodes by using the isolated root system of water spinach cultured in a non-exogenous medium. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is a kind of plant parasitic nematode that seriously harms crops. Its hosts are quite extensive. According to incomplete statistics, root-knot nematode hosts have exceeded 3,000 kinds of plants. The yield reduction is 10-20%, and the serious one can reach more than 75% (Pan Cangsang et al., 1994; Sasser, 1983). [0003] The four most common root-knot nematodes are: Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949], root-knot nematode Javan [M.javanica (Treub, 1885) Chitwood, 1949], root-knot nematode peanut [M. arenaria (Neal, 1889) Chitwood, 1949] and root-knot nematode [M. hapla Chitwood, 1949]. Root-knot nematodes not only cause the root syste...

AI Technical Summary

Problems solved by technology

Although the above-mentioned in vitro culture method can obtain a certain amount of nematodes, it still has certain limitations, such as complicated operation, high cost, and containing exogenous organisms or hormones. Interference with test results

[0010] Hutangura et al. (1998) and Mitkowski et al. (2002) successfully cultured root-knot nematodes in isolated tomato roots and dandelion isolated roots cultured in media without exogenous organisms and exogenous hormones, but the reproduction rate remained the same. Very low (47 root knots and 11 ± 3 oocysts produced respectively), difficult to apply in practice

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