Method for preparing recombinant human interleukin-11

A technology of interleukin and fusion protein, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., to achieve the effects of avoiding the renaturation step of inclusion bodies, improving recovery rate and efficient purification
CN102140487AInactive Publication Date: 2011-08-03DONGGUAN TAILI BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DONGGUAN TAILI BIOTECH
Publication Date
2011-08-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for preparing recombinant human interleukin-11 (rhIL). The method comprises the following steps: 1) providing a fusion protein, wherein from the N-end to the C-end, the fusion protein is thioredoxin-(His)6-proteolytic enzyme recognition site-rhIL-11 or (His)6-thioredoxin-proteolytic enzyme recognition site-rhIL-11; 2) using the proteolytic enzyme to perform enzyme cutting to the fusion protein and obtain an enzyme cutting product, wherein the enzyme cutting product contains thioredoxin and rhIL-11; and 3) using the nickel ion chelate affinity column chromatography to purify the enzyme cutting product, and performing gradient elution to the column to obtain rhIL-11, wherein the thioredoxin and the rhIL-11 are both adsorbed on the column. By adopting the method provided by the invention, the rhIL-11 can be purified rapidly, conveniently and efficiently and the recovery rate can be greatly increased.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular, the invention relates to a method for preparing recombinant human interleukin-11. Background technique

[0002] Interleukin-11 (Interleukin-11, IL-11) was first discovered in 1986 by D.A.Williams et al. of Harvard Medical School. IL-11 is a multifunctional cytokine, and its most basic function lies in the regulation of hematopoiesis. It stimulates the differentiation of human hematopoietic erythrocytes and megakaryocyte precursors and induces the generation and maturation of megakaryocytes, ultimately leading to an increase in the number of platelets. In addition, it also has various biological activities such as promoting the recovery of digestive tract epithelial damage and regulating adipocyte differentiation (Du X.X., Williams D.A., Blood, 1997, 89(11): 3897-3908). In 1990, Paul S.R. et al. (Proc.Natl.Acad.Sci.USA, 1990, 87:7512-7516) cloned the cDNA of this cytokine, and t...

Claims

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