7results about How to "High purification yield" patented technology

The present invention relates to a method for purification of botulinum toxin with improved purification purity and yield. When the method for purification of botulinum toxin of the present invention is used, botulinum toxin can be purified with a very high yield.

The invention relates to the technical field of medicine purification, and discloses a method for separating and purifying uridine-5 '-diphosphate-N-acetyl-glucosamine, which comprises the following steps: S1, loading a sample: filtering a uridine-5'-diphosphate-N-acetyl-glucosamine crude product, and loading the filtered uridine-5 '-diphosphate-N-acetyl-glucosamine crude product into a chromatographic column; s2, elution is carried out, wherein the uridine-5 '-diphosphate-N-acetyl-glucosamine adsorbed in the chromatographic column is eluted with an eluent; s3, after collecting, gathering and eluting, carrying out segmented collection, and gathering the component liquid meeting the target peak value requirement; the separation and purification method of uridine-5 '-diphosphate-N-acetyl-glucosamine is simple and convenient, the purification period is short, only a salt solution is used as a mobile phase in the whole purification process, no organic solvent is needed, the method is safe, pollution-free and very low in cost, the used stationary phase can be reused, and the method is suitable for industrial production. The method can be used for large-scale production, the environmental protection pressure and the production cost are greatly reduced, and a better use prospect is brought.

The application discloses a synthesis method of 9-bromoanthracene and belongs to the technical field of organic synthesis. Anthracene is used as raw material, inorganic bromide salt is used as a bromine source, hydrogen peroxide is used as an oxidant, and 9-bromoanthracene is synthesized in a mixed solvent under the catalysis of a composite Lewis acid with high selectivity; the crude product is purified through step-by-step fractional recrystallization to obtain a high-purity product. Through the synergistic effect of the composite catalytic system and the mixed solvent, the generation of the difficult-to-remove by-product 9,10-dibromoanthracene is inhibited from the source, and the efficient separation of the product and the key impurities can be realized without column chromatography by combining the gradient cooling fractional crystallization process. The process is green and safe, simple to operate, low in cost and easy to industrialize, and the prepared 9-bromoanthracene can meet the application requirements in high-end fields such as OLED photoelectric materials.

The invention relates to the technical field of medicine purification, and discloses a method for separating and purifying uridine diphosphate glucuronic acid, which comprises the following steps: S1, loading a sample: filtering a uridine diphosphate glucuronic acid crude product, and loading the filtered uridine diphosphate glucuronic acid crude product into a chromatographic column, the filler of the chromatographic column being a monodisperse microsphere of which the matrix is polyacrylate or polystyrene-divinyl benzene; s2, elution: eluting the uridine diphosphate glucuronic acid adsorbed in the chromatographic column by using an eluent; and S3, collection and gathering: carrying out segmented collection after elution, and gathering the component liquid meeting the target peak value requirement. The separation and purification method of uridine diphosphate glucuronic acid is simple and convenient, has a short purification period, only uses a salt solution as a mobile phase in the whole purification process, does not need to use any organic solvent, and is suitable for large-scale production. The method is safe, pollution-free and very low in cost, the used stationary phase can be reused, the method can be used for large-scale production, the environmental protection pressure and the production cost are greatly reduced, and a better use prospect is brought.

The invention discloses a method for synthesizing anomaline. The compound is one of effective anti-malarial components in a traditional Chinese medicine antifebrile dichroa. According to the method, trans-dichroine is taken as an initial raw material, and a cis-dichroine product is prepared through two-step reaction of benzylation and debenzylation. The synthesis process is simple, the used materials are easy to obtain, and the obtained product is high in purity and controllable in quality.

The invention relates to the technical field of medicine purification, and discloses a uridine diphosphate glucose separation and purification method, which comprises the following steps: S1, loading: filtering a uridine diphosphate glucose crude product, loading the filtered uridine diphosphate glucose crude product into a chromatographic column, and taking polystyrene-divinyl benzene monodisperse microspheres as a matrix as a filler of the chromatographic column; s2, elution: eluting the uridine diphosphate glucose adsorbed in the chromatographic column by using an eluent; and S3, collecting and gathering: carrying out segmented collection on the eluted uridine diphosphate glucose solution, and gathering the component liquid meeting the target peak value requirement. The separation and purification method of uridine diphosphate glucose is simple and convenient, the purification period is short, the requirement for high purity of uridine diphosphate glucose can be met only through one-step chromatographic purification, the purity reaches 99% or above, the purification yield is high and stable, and better use prospects are brought.

The invention discloses a bioengineering-based elastin-like protein purification method, which comprises: 1) obtaining an engineering bacterium thallus expressing elastin-like protein, and re-suspending the thallus by using a buffer solution to obtain a thallus re-suspension; 2) adding lysozyme and DNA enzyme into the thallus resuspension, heating to 35-55 DEG C, and stirring for 0.5-2 hours to obtain a thallus lysate; 3) rapidly cooling to 2-10 DEG C, and centrifuging to obtain clear supernate; 4) purifying the supernate through cation exchange chromatography to obtain a protein solution; 5) performing ultrafiltration desalting treatment on the protein liquid to obtain a pure protein aqueous solution; and 6) freeze-drying to obtain the protein. According to the method, the pure protein can be obtained by purifying the protein twice in a thermal cracking mode, the protein recovery efficiency is improved, the production cost is low, meanwhile, efficient purification of the K protein independent of His tag peptide is achieved, and the method is suitable for industrial production.