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Method of preparing veralkanol from Chinese medicinal herb plant polygonum cuspidatum

A technology of resveratrol and Chinese herbal medicine, applied in the field of preparation of resveratrol, can solve the problems of cumbersome operation and low product recovery rate, and achieve the effect of enriching plant resources, low cost and simple steps

Inactive Publication Date: 2006-01-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of resveratrol in plants mainly adopts the method of combining multi-step silica gel column chromatography and high performance liquid chromatography. The operation is often cumbersome and the recovery rate of the product is relatively low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 500g of dried Polygonum cuspidatum, mash it, heat and reflux three times with 95% ethanol at 80°C for 2 hours each time, filter the mixed solvent, concentrate in vacuo to a paste, dissolve the paste with 100mL distilled water by ultrasonic wave, and use Extract 6 times with equal volume of ethyl acetate, recover solvent, concentrate to dryness, dissolve in 50% methanol, use cellulose as stationary phase, use water, 10%, 30%, 50% and 70% methanol solution as mobile phase in turn Carry out column chromatography elution, collect detected resveratrol, vacuum freeze-dry, obtain resveratrol 2.7544g, carry out gas chromatography-mass spectrometry detection to the purified sample, find its retention time, molecular weight and resveratrol The standard samples are consistent.

[0020] Implementation effect: through this method, not only the cost is reduced, but also the yield of resveratrol is increased, which is 0.55%

Embodiment 2

[0022] Take 500g of dried Polygonum cuspidatum, mash it, heat and reflux three times with 70% ethanol at 60°C for 2 hours each time, filter the mixed solvent, concentrate in vacuum to a paste, use ultrasonic waves to dissolve the paste with 75mL distilled water, use etc. Extracted 6 times with ethyl acetate by volume, recovered the solvent, concentrated to dryness, dissolved in 50% methanol, used cellulose as the stationary phase, and used water, 10%, 30%, 50% and 70% methanol solution as the mobile phase in sequence Eluted by column chromatography, the detected resveratrol was collected, and vacuum freeze-dried to obtain 2.1217g of resveratrol. The purified sample was detected by gas chromatography, and its retention time, molecular weight and resveratrol label were found. so consistent.

[0023] Implementation effect: through this method, not only the cost is reduced, but also the yield of resveratrol is increased to 0.42%.

Embodiment 3

[0025] Take 500g of dried Polygonum cuspidatum, mash it, heat and reflux three times with 50% ethanol at 25°C for 2 hours each time, filter the mixed solvent, concentrate in vacuo to a paste, dissolve the paste with 50mL of distilled water by ultrasonic wave, and use an equal volume of Extracted 6 times with ethyl acetate, recovered the solvent, concentrated to dryness, dissolved in 50% methanol, used cellulose as the stationary phase, and used water, 10%, 30%, 50% and 70% methanol solution as the mobile phase to carry out column Chromatographic elution, the detected resveratrol was collected, and vacuum freeze-dried to obtain 1.9102g of resveratrol. The purified sample was detected by gas chromatography, and its retention time, molecular weight and resveratrol standard sample were found. unanimous.

[0026] Implementation effect: through this method, not only the cost is reduced, but also the yield of resveratrol is increased to 0.38%.

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PUM

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Abstract

The invention provides a method for preparing veralkanol giant knotweed rhizome a Chinese medicinal herbal plant, which consists of collecting giant knotweed rhizome, drying and preserving, using ethanol-water mixed solvent as extracting agent, heating and extracting through return flow, concentrating the mixed solvent extract into grease form substance through vacuum rotary concentration, thus obtaining giant knotweed rhizome crude extract, dissolving the grease form substance with distilled water, extracting the solution with equal-volume of acetic ester, drying the acetic ester through vacuum rotary concentration, dissolving with methanol, isolating and purifying with column chromatography, using cellulose as the fixed phase in column chromatography, carrying out column chromatography elution with methanol, collecting the eluent, concentrating the collected veralkanol, and freeze drying. The yield of the veralkanol is 0.4%.

Description

technical field [0001] The invention relates to a method for preparing resveratrol, in particular to a method for preparing resveratrol from Chinese herbal medicine Polygonum cuspidatum. It belongs to the field of traditional Chinese medicine preparation. Background technique [0002] Polygonum Cuspidatum Sieb.et Zucc is a traditional Chinese medicine in China, mainly distributed in East China, Central South and Liaoning, Shaanxi, Gansu, Sichuan, Guizhou, Yunnan and other places. It has the effects of promoting blood circulation and relieving pain, clearing heat and promoting dampness, quenching thirst and resolving phlegm. Knotweed contains resveratrol (resveratrol), the chemical name of resveratrol is 3,4',5-trihydroxy-trans-stilbene (3,4',5-trihydroxystilbene), molecular formula C14H1203, molecular weight 228 Since the first discovery of this ingredient in 1940, it has been valued by the medical community. So far, research has shown that resveratrol has anti-tumor, anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/21C07C37/70
Inventor 代光辉姚柳利
Owner SHANGHAI JIAO TONG UNIV
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