Method for extracting and separating flavonoid compounds from hypericum japonicum

Abstract

The invention discloses a method for preparing flavonoid compounds from hypericum japonicum by means of extracting and separating. The method includes steps of adding hypericum japonicum medicinal materials into water with the quantity of 8-12 times the quantity of the hypericum japonicum medicinal materials in each procedure, extracting the hypericum japonicum medicinal materials for 0.5-1.5 hours under heating conditions in each procedure, extracting the hypericum japonicum medicinal materials by 2-4 times, filtering extracted liquid to obtain filter liquid, collecting and combining the filter liquid, and concentrating the filter liquid to obtain thick paste, namely, an extract A, of the hypericum japonicum medicinal materials; adding a certain quantity of ethyl alcohol or methanol into the extract A until the concentration of the ethyl alcohol or methanol is 60%-90%, uniformly mixing the ethyl alcohol or methanol with the extract A to obtain mixed liquid, allowing the mixed liquid to stand still and layer, then filtering upper liquor layers, recycling solvents and concentrating the upper liquor layers to obtain a semi-solid extract which is an extract B; adding the extract B into water with the quantity of 0.5-2.0 times the quantity of the extract B, uniformly stirring the extract B, filtering insoluble materials to obtain filter liquid, allowing the filter liquid to stand at the temperature of 0-30 DEG C for 2 hours at least, dissolving out precipitates, filtering the filter liquid, collecting filter cake and drying the filter cake to obtain the flavonoid compounds. The relative density of the thick paste of the hypericum japonicum medicinal materials is 1.10-1.40. The method has the advantages that the high-content flavonoid compounds can be extracted from the hypericum japonicum by the aid of 4 steps, the method is simple, convenient, practical and environmental friendly, and industrial production can be facilitated.

Description

technical field [0001] The invention belongs to the technical field of extracting effective parts of Chinese medicinal materials, and in particular relates to a method for extracting and separating flavonoids from Tianjihuang. Background technique [0002] Tianjihuang is the dried whole herb of Hypericum japonicum Thunb. in the genus Hypericum of the Garciniaceae family. It is an annual herb that grows wild in wild wetlands. Traditional Chinese medicine in my country is sweet, slightly bitter, and cool in nature, and it belongs to the liver, gallbladder, and large intestine meridian. , diarrhea, dysentery, snake bites, carbuncle furuncle swelling and pain, bruises, etc. Modern pharmacological experiments have proved that Tianjihuang has various pharmacological effects, including anti-tumor, immune regulation, antibacterial, liver protection, anti-virus, hemostasis, anti-malarial and so on. It is clinically used to treat viral hepatitis. At present, Tianjihuang extract has be...

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Problems solved by technology

[0004] At present, the extraction process of flavonoids in Tianjihuang is complicated and the extraction cost is high. Patent document CN1943605A discloses a preparation method of Tianjihuang extract, the total flavonoid content is greater than 50%. However, this method uses ceramic membranes and organic Membrane filtration leads to complex extraction operations and high costs, which are difficult to apply to industrial production; especially the purification steps after alcohol precipitation in the water extraction and alcohol precipitation method, the process is more complicated and the steps are more cumbersome

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