The invention relates to a preparation method of a medicine raw material, and especially relates to an extraction method of streptomycin sulfate. According to the method, the extraction process is simplified, the production efficiency is improved, the invested cost is reduced, the clarity of a filtrate is good, the activated carbon amount is small, the yield is high, the product color grade is good, and the pass rate is high. The method comprises the following steps: A, acidifying a streptomycin sulfate fermentation liquid to the pH of 2.0-4.0, adding a flocculant, stirring, and filtering the liquid with a ceramic membrane; B, adjusting the pH of the filtrate obtained in the step A with sodium hydroxide to 6.0-8.0, and carrying out dynamic adsorption with resin; C, desorbing the saturated resin in the step B with a sulfuric acid aqueous solution; D, desalting and neutralizing the desorption liquid obtained in the step C with a mixed bed to obtain a refined liquid; E, adding the refined liquid obtained in the step D to activated carbon, and filtering; F, concentrating the decolorized liquid obtained in the step E with a nanofiltration membrane; G, removing bacterial endotoxin and pyrogen with an ultrafiltration membrane; and H, spray-drying the product obtained in the step G to obtain the finished product.