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Application of 4-vinylphenol to preparing nematode insecticide

A vinylphenol and insecticide technology is applied in the application field of 4-vinylphenol in the preparation of nematode insecticides, and can solve problems such as difficulty in control work, lack of drug-resistant effective vaccines, and the like

Inactive Publication Date: 2017-08-18
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the emergence of drug resistance and the lack of effective vaccines have brought difficulties to the prevention and control work, and new drugs are urgently needed to be developed

Method used

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  • Application of 4-vinylphenol to preparing nematode insecticide
  • Application of 4-vinylphenol to preparing nematode insecticide
  • Application of 4-vinylphenol to preparing nematode insecticide

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0023] The application of 4-vinylphenol (4-vinylphenol) in the preparation of root-knot nematode insecticide, the application process is as follows:

[0024] The root-knot nematodes infected with root-knot nematodes were picked from the roots of tomatoes, and egg masses were placed in a 96-well plate to incubate at 20°C to observe the hatching of root-knot nematodes. Rinse the nematodes with sterile water, add 200 μL of 4-vinylphenol solution with concentrations of 10, 11, 12, 13, 14, and 15 μg / mL in the 96-well plate, add 2 μL of chloramphenicol (30 mg / mL) and 30 The head nematodes and purified water were used as control, and each group had 3 repetitions. Placed in a 20°C incubator for cultivation, observe the experimental results ( figure 1 ).

[0025] Nematode mortality rate = number of dead nematodes / number of tested nematodes*100%

[0026] Corrected death rate=(nematode death rate of treatment group-nematode death rate of control group) / (1-nematode death rate of contro...

experiment example 2

[0033] The application of 4-vinylphenol (4-vinylphenol) in the preparation of Caenorhabditis elegans insecticide is as follows:

[0034] Add 200 μL of 4-vinylphenol solution with a concentration of 50, 75, 100, 125, 150, and 175 μg / mL in the 96-well plate, add 2 μL of chloramphenicol (30 mg / mL) and about 30 Caenorhabditis elegans nematodes of J2 stage, and pure water as a control, 3 repetitions per set. Place them in a 20°C incubator and observe the experimental results.

[0035] Nematode mortality rate = number of dead nematodes / number of tested nematodes*100%

[0036] Corrected death rate=(nematode death rate of treatment group-nematode death rate of control group) / (1-nematode death rate of control group)*100%

[0037] Table 2. The activity of 4-vinylphenol against Caenorhabditis elegans

[0038]

[0039] The nematodes in the control group grew normally, and the median lethal concentrations of 4-vinylphenol to Caenorhabditis elegans were 103.33ug / ml (48h) and 70.30ug / m...

experiment example 3

[0041] The application of 4-vinylphenol (4-vinylphenol) in the preparation of Haemonchus contortus insecticide is as follows:

[0042] Obtain and incubate the third-stage larvae of Haemonchus contortus from the feces of infected sheep, wash the nematodes with normal saline, treat them at 38°C for 25-30 minutes for sheathing, then wash the nematodes with LB culture medium, and add 98 μL to a 96-well plate Add 2 μL of 4-vinylphenol solution to the LB solution containing about 100 larvae to make the final concentration reach 1 mg / mL, and use the LB solution as a control, with 3 repetitions in each group. Place them in an incubator at 38°C, and observe the experimental results after 72 hours.

[0043] The growth of nematodes in the control group was normal, and under the treatment of 1 mg / mL 4-vinylphenol, all the third-stage larvae of Haemonchus contortus died. 4-Vinylphenol has strong insecticidal activity against zoopathogenic nematode Haemonchus contortus ( figure 2 ).

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Abstract

The invention belongs to the technical field of agricultural microbiology and discloses an application of 4-vinylphenol to preparing nematode insecticide. That the 4-vinylphenol has a function of killing nematode is first proposed in the invention; the 4-vinylphenol has a very good effect of killing and inhibiting south meloidogyne, haemonchus contortus, caenorhabditis elegans and south meloidogyne eggs; the 4-vinylphenol has the advantages of high efficiency, low toxicity, good specificity and good persistence; and a new selection is provided for preparing novel preparations for preventing and treating plant pathogenic nematode and animal pathogenic nematode.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to the application of 4-vinylphenol in the preparation of nematode insecticides. Background technique [0002] As a common parasitic nematode, plant pathogenic nematodes have the characteristics of multiple hosts, difficult control and strong drug resistance, which have caused serious harm to agricultural production. Among them, plant root-knot nematodes and plant cyst nematodes are the most harmful to crops. It also has the widest distribution (Curtis 2007). Root-knot nematode (Meloidogyne spp.) is an endoparasitic nematode that colonizes the root system of plants, belonging to the order Tylenchida, family Heteroderidae, subfamily Meloidogyninae, genus Meloidogyne . In my country, root-knot nematode incognita is the most widely distributed and most harmful. At present, nematode control technologies at home and abroad mainly include the following met...

Claims

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Application Information

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IPC IPC(8): A01N31/08A01P5/00
CPCA01N31/08
Inventor 张吉斌邵宗泽黄典蔡珉敏李光玉郑龙玉孙风芹喻子牛余晨胡敏高冲
Owner HUAZHONG AGRI UNIV
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