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Compound microorganism bacterium agent for preventing and treating meloidogyne and preparation method thereof

A technology of compound microbial inoculants and root-knot nematodes, which is applied in the fields of botanical equipment and methods, biocides, nematicides, etc., can solve the problems of insufficient control effect and low control effect.

Inactive Publication Date: 2012-07-04
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the problems of low control effect and unstable control effect of existing biocontrol agents in the prevention and treatment of root-knot nematodes, and to provide a method for preventing and controlling root-knot nematodes with high control effect and stable control effect. Compound microbial agent

Method used

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  • Compound microorganism bacterium agent for preventing and treating meloidogyne and preparation method thereof
  • Compound microorganism bacterium agent for preventing and treating meloidogyne and preparation method thereof
  • Compound microorganism bacterium agent for preventing and treating meloidogyne and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of Preliminary Example 1 Verticillium chlamydoides fermented liquid

[0017] 1. After activating the strain of Verticillium chlamydoides, carry out shake flask seed culture;

[0018] The composition (by weight percentage) of shaking flask seed culture medium:

[0019] Glucose 3-5%

[0020] Soybean flour 1.5-2.5%

[0021] Dipotassium hydrogen phosphate 0.05-0.1%

[0022] Magnesium sulfate 0.05-0.1%

[0023] Water 92.3%-95.4%

[0024] Liquid fermentation conditions: put 200ml of seed culture medium in a 500ml Erlenmeyer flask, inoculate the spore suspension with an inoculum size of 1.5-3% after autoclaving, place on a shaker at 24-28°C at 150-200r / min and cultivate for 36-72h.

[0025] 2. Secondary liquid expansion culture (by weight percentage):

[0026] Glucose 2-3%

[0027] Soybean flour 1-2%

[0028] Cornmeal 1-2%

[0029] Dipotassium hydrogen phosphate 0.1-0.2%

[0030] Magnesium sulfate 0.1-0.2%

[0031] Water 92.6%-95.8%

[0032] After the a...

Embodiment 2

[0045] Preparation of Preparatory Example 2 Paecilomyces lilacinus (P.lilacinus) fermented liquid

[0046] 1. After activating the Paecilomyces lilacinus species, carry out shake flask seed culture;

[0047] The composition (by weight percentage) of shaking flask seed culture medium:

[0048] Glucose 1.5-2%

[0049] Sodium nitrate 0.4-0.6%

[0050] Ammonium sulfate 0.2-0.3%

[0051] Soy flour 0.2-0.3%

[0052] Dipotassium hydrogen phosphate 0.1-0.2%

[0053] Magnesium sulfate 0.1-0.2%

[0054] Water 96.4%-97.5%

[0055] Liquid fermentation conditions: put 200ml of seed culture medium into a 500ml Erlenmeyer flask, inoculate the spore suspension with an inoculum size of 1.5-3% after autoclaving, place on a shaker at 24-28°C at 150-200r / min and cultivate for 18-36h.

[0056] 2. Secondary liquid expansion culture (by weight percentage):

[0057] Glucose 3-5%

[0058] Sodium nitrate 0.4-0.6%

[0059] Ammonium sulfate 0.2-0.3%

[0060] Soy flour 0.2-0.3%

[0061] Dipotass...

Embodiment 3

[0081] The preparation of embodiment 3 composite microbial bacterial agents (for testing bacterial agent 3)

[0082] Mix the Verticillium chlamydota fermented liquid prepared in Preliminary Example 1 with the Paecilomyces lilacinus fermented liquid prepared in Preliminary Example 2 to obtain a mixed bacterial agent; The spore concentration was controlled as follows: the number of spores of Verticillium chlamydoides was 1.0×10 7 spores / ml, the number of spores of Paecilomyces lilacinus is 1.0×10 9 pieces / ml.

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Abstract

The invention discloses a compound microorganism bacterium agent for preventing and treating meloidogyne and a preparation method of the compound microorganism bacterium agent. The compound microorganism bacterium agent comprises the following ingredients: paecilomyceslilacinus (Thom) samson fermentation liquid and verticilliumchlamydosporium fermentation liquid. The field meloidogyne prevention and treatment test results show that after paecilomyceslilacinus (Thom) samson microbial inoculums and verticilliumchlamydosporium microbial inoculums are compounded, the paecilomyceslilacinus (Thom) samson microbial inoculums and the verticilliumchlamydosporium microbial inoculums show the very obvious cooperative synergia meloidogyne prevention and treatment effect through being compared with the single paecilomyceslilacinus (Thom) samson microbial inoculums or the verticilliumchlamydosporium microbial inoculums, and the prevention effect on the meloidogyne is obviously improved. In addition, the integral prevention effect of the compound microorganism bacterium agent on the meloidogyne is also more and more stable.

Description

technical field [0001] The invention relates to a biological agent for preventing and controlling plant parasitic nematodes in soil, and in particular to a compound microbial agent for preventing and controlling root-knot nematodes. The present invention also relates to a preparation method and application of the compound microbial agent, belonging to root-knot The field of biological control of nematodes. Background technique [0002] Plant parasitic nematodes are the second largest group of crop pathogens after fungi. They are widely distributed and can parasitize and harm a variety of crops. Root-knot nematode is a kind of economically important plant obligate parasitic pathogen, and most of the nematicides used in production are highly toxic chemical pesticides with high toxicity, which not only affects human health, but also poses a potential threat to the environment . In recent years, some soil nematode chemicals have been banned, so the biological control of plant ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P5/00
Inventor 王曦茁汪来发朴春根李永朱天辉杨佐忠林彩丽田国忠郭民伟
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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