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Paecilomyces lilacinus wettable powder, preparation method and application thereof

A technology of wettable powder, Paecilomyces lilacinus, applied in the directions of botanical equipment and methods, applications, biocides, etc.

Active Publication Date: 2015-03-04
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many types of wetting agents and dispersants used to prepare pesticide wettable powders, but there are significant differences in the effects of different types of wetting agents or dispersants on the spore germination or mycelial growth of Paecilomyces lilacinus, Even if it is the same kind of wetting agent or dispersant, the difference in the dosage will have opposite effects on the germination of Paecilomyces lilacinus spores or the growth of hyphae

Method used

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  • Paecilomyces lilacinus wettable powder, preparation method and application thereof
  • Paecilomyces lilacinus wettable powder, preparation method and application thereof
  • Paecilomyces lilacinus wettable powder, preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0047] The preparation of embodiment 1 wettable powder

[0048] (1) Weigh each raw material by the following weight (unit: kg): Paecilomyces lilacinus spore powder (1.0×10 11 CFU / g or more) 94.45, diatomaceous earth 5, PEG 0.5, D1002 0.05; (2) Mix Paecilomyces lilacinus spore powder and diatomaceous earth evenly and then pulverize through a 325 mesh sieve; mix the pulverized product with PEG and After D1002 is mixed evenly, it is finely ground with a jet mill, and mixed evenly, that is to say.

[0049] Use GB / T5451 to test the wettability of the product; use GB / T14825-2006 to test the suspension rate of the product; use GB / T1600-2001 to test the product's moisture; use GB / T16150 to test the product's fineness; use GB / T1601-93 Detect the heat storage stability of the product; after testing, the various properties of the wettable powder prepared in this embodiment are as follows: wettability: 112 seconds of total wetting time; suspension rate: 78%; product moisture is less than...

Embodiment 2

[0050] The preparation of embodiment 2 wettable powder

[0051] (1) Weigh each raw material by the following weight (unit: kg): Paecilomyces lilacinus spore powder (1.0×10 11 CFU / g or more) 93.9, diatomaceous earth 5, PEG 0.1, D1002 1.0; (2) Mix Paecilomyces lilacinus spore powder and diatomaceous earth evenly and then pulverize through a 325 mesh sieve; mix the pulverized product with PEG and After D1002 is mixed evenly, it is finely ground with a jet mill, and mixed evenly, that is to say. After testing, the properties of the wettable powder prepared in this example are as follows: wettability: total wetting time 102 seconds; suspension rate: 82%; product moisture is less than 3%; pH value 6-8; average particle size : 3-6 microns; Stability: Stored at 54±2°C for 14 days, the suspension rate and wettability are both qualified.

Embodiment 3

[0052] The preparation of embodiment 3 wettable powder

[0053] (1) Weigh each raw material by the following weight (unit: kg): Paecilomyces lilacinus spore powder (1.0×10 11 CFU / g or more) 91.0, diatomaceous earth 5, PEG 2, D1002 2; (2) Mix Paecilomyces lilacinus spore powder and diatomaceous earth evenly and then pulverize through a 325 mesh sieve; mix the pulverized product with PEG and After D1002 is mixed evenly, it is finely ground with a jet mill, and mixed evenly, that is to say. After testing, the properties of the wettable powder are as follows: wettability: total wetting time 91 seconds; suspension rate: 87%; product moisture is less than 3%; pH value 6-8; average particle size: 3-6 microns; Stability: Stored at 54±2°C for 14 days, the suspension rate and wettability are both qualified.

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Abstract

The invention discloses paecilomyces lilacinus wettable powder, a preparation method and application thereof. The paecilomyces lilacinus wettable powder comprises the following components of paecilomyces lilacinus spore powder or fermentation liquor, a vector, a wetting agent and a dispersing agent, wherein the wetting agent is selected from any one or more of D1002, NNO or sodium lignin sulfonate; and the paecilomyces lilacinus wettable powder also comprises an ultraviolet protection agent or / and a synergist. According to the paecilomyces lilacinus wettable powder, the traditional wetting agents and dispersing agents are screened and optimized to obtain a specific species and a dosage having a smaller influence on the growth of the paecilomyces lilacinus; in addition, the ultraviolet protection agent for preventing paecilomyces lilacinus spores from being damaged by ultraviolet and the synergist for promoting plants to take roots or increasing the meloidogyne killing effect are further screened and obtained. The invention further provides the preparation method of the paecilomyces lilacinus wettable powder and the application of the wettable powder in preventing and controlling plant meloidogyne.

Description

technical field [0001] The invention relates to a biological pesticide preparation, in particular to a wettable powder of Paecilomyces lilacinus (P.lilacinus) for controlling root-knot nematodes and a preparation method thereof, which belongs to the field of biological pesticide preparations of Paecilomyces lilacinus. Background technique [0002] Paecilomyces lilacinus (P.lilacinus) is a resident fungus in the soil and the rhizosphere of various plants. It has a high parasitic rate to the root-knot nematode incognita, which can reach more than 80%. Paecilomyces lilacinus is a very important plant-parasitic nematode egg-parasitic fungus, which has been widely used to control harmful plant-parasitic nematodes such as root-knot nematodes and cyst nematodes. As one of the important biocontrol bacteria for the control of plant parasitic nematodes, the rapid germination of Paecilomyces lilacinus spores can directly affect the pathogenicity of the strain to the host, and can great...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N43/38A01N39/04A01N37/10A01N37/04A01N37/40A01N25/14A01P5/00
Inventor 朴春根汪来发王曦茁郭志斌李永郭民伟
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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