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Composite microbial agent for preventing and controlling Meloidogyne arenria, and preparation method thereof

A technology of compound microbial agent and compound agent, applied in botany equipment and methods, biocides, nematicides, etc., can solve chemical hazards and other problems, and achieve good insecticidal effect

Inactive Publication Date: 2019-09-27
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparations for the prevention and treatment of peanut root-knot nematode are mostly chemical formulations, but there are consistent chemical hazards. It can be seen that the development of a microbial agent that can effectively prevent and control peanut root-knot nematode will play an important role in the green prevention and treatment of peanut root-knot nematode disease. of great significance

Method used

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  • Composite microbial agent for preventing and controlling Meloidogyne arenria, and preparation method thereof
  • Composite microbial agent for preventing and controlling Meloidogyne arenria, and preparation method thereof
  • Composite microbial agent for preventing and controlling Meloidogyne arenria, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The raw materials for the preparation of the composite microbial bacterial agent described in this embodiment include a composite microbial agent and a plant extract with a mass ratio of 1:3; wherein,

[0021] The composite bacterial agent is a mixed bacterial agent of Lactobacillus rhamnosus, Propionibacterium freunderi and Bacillus amyloliquefaciens with a mass ratio of 1:1:2;

[0022] The plant extract is prepared according to the following steps:

[0023] (1) Wash and peel the water chestnuts and burdock, and set aside; then mix the water chestnuts and burdock according to the mass ratio of 1:1, add water twice the amount of the total amount of raw materials, mix and make pulp, and set aside;

[0024] (2) Add cellulase accounting for 1 wt% of the total amount of the water chestnut and burdock raw materials to the above slurry, carry out enzymolysis treatment at room temperature for 3 hours, filter after inactivating the enzyme, collect the enzymolysis solution, spra...

Embodiment 2

[0027] The raw materials for the preparation of the composite microbial bacterial agent described in this embodiment include a composite microbial agent and a plant extract with a mass ratio of 1:8; wherein,

[0028] The composite bacterial agent is a mixed bacterial agent of Lactobacillus rhamnosus, Propionibacterium freunderi and Bacillus amyloliquefaciens with a mass ratio of 1:1:2;

[0029] The plant extract is prepared according to the following steps:

[0030] (1) Take the water chestnuts and burdock, wash and peel them, and set aside; then mix the water chestnuts and burdocks according to the mass ratio of 1:2, add 5 times the amount of water of the total amount of raw materials, mix and make pulp, and set aside;

[0031] (2) Add cellulase accounting for 2wt% of the total amount of water chestnut and burdock raw materials to the above slurry, carry out enzymolysis treatment at room temperature for 3 hours, filter after inactivating the enzyme, collect the enzymolysis so...

Embodiment 3

[0034] The raw materials for the preparation of the composite microbial bacterial agent described in this embodiment include a composite microbial agent and a plant extract with a mass ratio of 3:3; wherein,

[0035] The composite bacterial agent is a mixed bacterial agent of Lactobacillus rhamnosus, Propionibacterium freunderi and Bacillus amyloliquefaciens with a mass ratio of 1:1:2;

[0036] The plant extract is prepared according to the following steps:

[0037] (1) Wash and peel the water chestnuts and burdock, and set aside; then mix the water chestnuts and burdock according to the mass ratio of 1:1, add 3 times the amount of water of the total amount of raw materials, mix and make pulp, and set aside;

[0038] (2) Add cellulase accounting for 2wt% of the total amount of water chestnut and burdock raw materials to the above slurry, carry out enzymolysis treatment at room temperature for 3 hours, filter after inactivating the enzyme, collect the enzymolysis solution, spra...

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Abstract

The invention belongs to the technical field of pest prevention and control, particularly relates to a composite microbial agent for preventing and controlling Meloidogyne arenria, and further discloses a preparation method of the composite microbial agent. The composite microbial agent for preventing and controlling Meloidogyne arenria, adopting selected composite microbes and plant extract as effective components, facilitates the synergistic effects of Lactobacillus rhamnosus, Propionibacterium freudenreichii and Bacillus amyloliquefaciens and the effects of the enzymatic hydrolysate of water chestnuts and burdocks, all the components coordinate with each other to exert the insecticidal effect, and indoor test and field test of the composite microbial agent prove that the composite microbial agent of the invention has a good killing effect on the Meloidogyne arenria, and does not affect the growth of peanuts at all.

Description

technical field [0001] The invention belongs to the technical field of pest control, and in particular relates to a compound microbial bacterial agent for preventing and controlling peanut root-knot nematode, and further discloses a preparation method thereof. Background technique [0002] Peanut root-knot nematode disease, also known as peanut nematode disease, is commonly known as ground yellow disease, ground drop disease, dwarf yellow disease, yellow seedling disease, etc. The second-instar larvae of root-knot nematodes will invade the young root tips of peanuts and form irregular root knots. The nematodes will drill into the middle column of peanut roots, causing root fluid seepage and nutrient loss, which will easily cause secondary diseases. Root-knot nematodes colonize and feed and stimulate root cell proliferation, and continuously damage the root tips of new roots, causing secondary roots to form clusters (root clusters), making it difficult for them to absorb nutr...

Claims

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Application Information

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IPC IPC(8): A01N65/40A01N63/00A01P5/00
CPCA01N63/00A01N65/00A01N65/12A01N65/40
Inventor 高宗军李如美李瑞娟戴争
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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