20 results about "Heterodera" patented technology

The invention discloses a molecular marker for identifying a soybean variety resistant to cyst nematode disease and application, and belongs to the technical field of molecular marker-assisted breeding. The invention aims to more quickly and accurately screen the soybean variety resistant to cyst nematode disease. The invention provides a molecular marker for identifying a soybean variety resistant to cyst nematode disease. The 8382716 site in a Glyma. 19G051500 gene is G or C; the 846511th site in the Glyma. 19G051900 gene is A or G, and the 8466511th site in the Glyma. 19G051900 gene The 8522589 site in the Glyma. 19G052400 gene is A or C; the 8481473 site in the Glyma. 19G052100 gene is T or C; or the 181 site in the sequence as shown in SEQ ID NO.4 is T or C. And favorable reference basis and technical support are provided for soybean cyst nematode disease-resistant molecule assisted breeding.

The invention provides a primer combination for identifying a single-copy gene of Australian cyst nematode. The primer combination comprises an upstream outer primer HAUS-F3, a downstream outer primer HAUS-B3, an upstream inner primer HAUS-FIP, a downstream inner primer HAUS-BIP and a downstream loop primer HAUS-LB. The primer combination can specifically distinguish the Australian cyst nematode from other common cyst nematodes such as cereal cyst nematode, Philipse cyst nematode, corn cyst nematode and soybean cyst nematode, the detection limit is low, the detection time is short, and the detection result is identified by means of dye color development or electrophoretogram. The technology is easy to operate, good in sensitivity, high in specificity and high in reaction speed, the result can be directly observed without additional instruments, and the method has important significance in the aspects of rapid detection and early field diagnosis of the Australian cyst nematode.

The invention discloses a soybean cyst nematode No.5 physiological race resistance associated KASP molecular marker, a primer and application, and relates to the technical field of gene engineering. The soybean cyst nematode 5 physiological race resistance associated KASP molecular markers are SNP molecular markers SCN2.1, SCN2.2 and SCN2.3, the SCN2.1 has a pair of primers including a forward primer and a reverse primer, the SCN2.2 has a pair of primers including a forward primer and a reverse primer, the SCN2.3 has a pair of primers including a forward primer and a reverse primer, and the kit contains the primers of the KASP molecular markers. The KASP molecular marker is applied to the breeding of the soybean cyst nematode No.5 physiological race. The invention provides a set of complete technical solution which comprises a KASP molecular marker directly associated with resistance, a matched specific detection primer combination and a rapid detection and application method, early-stage, high-throughput and accurate identification of soybean anti-soybean cyst nematode 5 physiological race traits is realized, and the disease-resistant breeding efficiency is remarkably improved.

The invention provides an SNP molecular marker related to soybean cyst nematode disease resistance, a detection primer and application of the SNP molecular marker and the detection primer, and belongs to the technical field of plant breeding and molecular biology. The molecular marker is located at the 797th site of the ninth exon region of the soybean SNAP18 gene, and Ggt exists; a single nucleotide polymorphism (SNP). A specific KASP primer is designed on the basis of the site, the resistance of soybeans to cyst nematode can be accurately identified through genotype detection, and excellent phenotypes with scab-free characters can be screened at the same time. The method is high in detection accuracy, has the advantages of being accurate, rapid and low in cost, can be used for screening soybean cyst nematode resistance and / or scab-free excellent germplasm resources and molecular marker-assisted selective breeding, and remarkably improves the disease-resistant breeding efficiency.

Compositions and methods for conferring nematicidal activity upon bacteria, plants, plant cells, tissues, and seeds are provided. Methods for killing or controlling populations of nematode pests, such as cyst nematode species, for example, *Heterodera glycines* (soybean cyst nematode), root-knot nematodes, kidney-shaped nematodes, short-bodied nematode species, or lanceolate nematode populations, are also provided. These methods further include contacting the nematode pest with a pest-killing amount of a polypeptide containing a nematicidal toxin. Further methods include increasing plant yield by expressing genes of the embodiments in plants.

The object of the present invention is to provide a method for specifically and efficiently detecting the soybean cyst nematode, a serious pest of beans. [Solution] A method for detecting soybean cyst nematodes includes a step of performing a PCR amplification reaction of a target nucleic acid region of soybean cyst nematode using a soybean cyst nematode-specific primer set designed based on the sequence of the COI gene of the soybean cyst nematode's mitochondrial DNA.

The invention belongs to the technical field of plant molecular breeding, and discloses a molecular marker related to soybean cyst nematode disease resistance and application thereof. The CAPS molecular marker provided by the invention is simple to match and operate, and can quickly distinguish three genotypes of susceptibility, disease resistance and excellent phenotype and disease resistance and scabs. The molecular marker does not need expensive detection equipment, is simple and convenient to operate, low in cost and accurate in typing, is suitable for screening soybean cyst nematode-resistant filial generations, identifying transgenic lines and detecting the purity of disease-resistant varieties, can remarkably improve the breeding efficiency and shorten the breeding period, and provides a practical technical tool for soybean cyst nematode resistance breeding.

The invention belongs to the technical field of molecular biology and crop germplasm resource identification, and provides an application of a GmPDH1 gene and a protein coded by the GmPDH1 gene in resisting soybean cyst nematode No.4 race. The nucleotide sequence of the GmPDH1 gene CDS is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the gene is as shown in SEQ ID NO. 2. And the GmPDH1 positively regulates the resistance of the soybeans to the No.4 microspecies of the soybean cyst nematode. The method comprises the following steps: constructing an overexpression vector by using a pCAMBIA1302 vector, constructing a knockout vector by using a CRISPR-Cas9 editing technology, respectively transforming the overexpression vector and the knockout vector into soybean root systems, and inoculating the soybean root systems with soybean cyst nematode for identification. The result shows that the resistance of the soybean to the SCN4 is improved by overexpressing the soybean GmPDH1 gene, and the resistance of the soybean to the SCN4 is reduced by knocking out the GmPDH1 gene. And a gene resource is provided for soybean cyst nematode disease resistant genetic breeding of soybeans.

The invention belongs to the technical field of molecular marker-assisted selective breeding, and particularly relates to a method for breeding an anti-cyst nematode No.4 physiological race soybean variety. The invention provides a PCR (Polymerase Chain Reaction) primer combination for screening an anti-cyst nematode No.4 physiological race soybean variety, wherein the PCR primer combination comprises a first PCR primer group, a second PCR primer group and a third PCR primer group; the first PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 1-3; the second PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 4-6; and the third PCR primer group consists of primers with nucleotide sequences as shown in SEQ ID NO: 7-9. The invention further provides a method for breeding the cyst nematode-resistant No.4 physiological race soybean variety. When the method is used for marker-assisted selection, the efficiency and accuracy of soybean cyst nematode-resistant breeding can be greatly improved.

A polycyclic compound represented by formula (A) is a novel polycyclic compound that exhibits hatch-inducing activity against cyst nematodes. [In the formula, R1 represents a carboxy group or CH2CH=C(CH3)2.]

The subject invention describes a new method for the detection of root-knot nematodes (Meloidogyne spp.) at the egg stage isolated from soil. The described procedure enables the isolation of the eggs of root-knot nematode species directly from the soil and their subsequent use for their detection and identification without DNA extraction. In the procedure, an enzyme cocktail is used to release the DNA content from the material obtained by a specific purification procedure of the soil being tested and put into a real time PCR reaction, which detects the presence of nematodes of the Meloidogyne species. The procedure described here can be easily modified for the identification of other types of parasitic nematodes such as Globodera spp, Heterodera spp ili Pratylenchus spp.

The invention belongs to the technical field of plant genetic engineering, and provides an application of a GmGTT1 or GmTGA15 gene and a protein coded by the GmGTT1 or GmTGA15 gene in resisting soybean paratocyst nematode No.4 race. The nucleotide sequence of the GmGTT1 gene CDS is as shown in SEQ ID NO. 1, and the amino acid sequence coded by the gene is as shown in SEQ ID NO. 2; the nucleotide sequence of the GmTGA15 gene CDS is as shown in SEQ ID NO.3, and the amino acid sequence coded by the gene is as shown in SEQ ID NO.4. The over-expression of the soybean GmTGA15 or GmGTT1 gene can improve the tolerance of the soybean to the SCN4, and the knockout of the gene can reduce the resistance of the soybean to the SCN4. The discovery of the functions of the soybean GmTGA15 or GmGTT1 gene provides a gene resource for soybean cyst nematode disease resistance genetic breeding, and also provides a gene target for soybean SCN resistance molecular breeding.

The present invention discloses a method and system for monitoring soybean cyst nematode population dynamics based on the Internet of Things (IoT). The method comprises collecting pest detection data and monitoring data within a preset area for gene-driven pest control, preprocessing the pest detection data and monitoring data, performing target screening on the pest detection data to obtain target detection data, and performing control risk assessment on the target detection data based on the monitoring data to obtain a control ratio, performing causal analysis on the pest detection data based on gene drive to obtain an ecological risk, constructing an optimized growth degree function based on the control ratio and the ecological risk, constructing an IoT-based soybean cyst nematode population dynamics monitoring model based on the optimized growth degree function, inputting the data to be monitored into the IoT-based soybean cyst nematode population dynamics monitoring model, and outputting monitoring results. This method improves the accuracy of nematode population dynamics monitoring and can be directly applied to nematode population dynamics monitoring systems.

A Tralopyril derivative and use thereof are provided. The Tralopyril derivative may be used to control plant-parasitic nematodes, such as root-knot nematodes and cyst nematodes. The biological assay results show that, using the foliar spray method at a dosage only one twentieth of that used in the root-irrigation method, Tralopyril-β-aminobutyric acid exhibits higher potted-plant activity against root-knot nematodes in tomato, cowpea, and cucumber than cyclobutrifluram applied at the same concentration via the root-irrigation method. Tralopyril-β-aminobutyric acid also exhibits excellent control efficacy against tobacco root-knot nematodes when applied by both foliar spray and root-irrigation methods. Tralopyril-β-aminobutyric acid also exhibits certain control efficacy against cyst nematodes in soybean.

Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Further, methods for killing or controlling a nematode pest population, particularly a Pratylenchus spp., e.g., Pratylenchus brachyurus, Pratylenchus penetrans, Pratylenchus scribneri, Pratylenchus neglectus, Pratylenchus zeae, Belonolaimus longicaudatus, Hoplolaimus galeatus, Helicotylenchus dihystera, Helicotylenchus pseudorobustus, or Xiphinema Americanum, root knot nematode, reniform nematode, soybean cyst nematode or Lance nematode population, are provided. The methods include contacting the nematode pest with a pesticidally-effective amount of a polypeptide comprising a nematicidal toxin. Further included are methods for increasing yield in plants by expressing the genes of the embodiments in plants.

The invention discloses application of a pyrimidine heterocyclic compound in prevention and control of plant parasitic nematodes, and belongs to the technical field of prevention and control of plant parasitic nematodes. The pyrimidine heterocyclic compound is 2-(5-bromopyrimidine-2-yl)-5-(6-ethyl-5-fluoropyrimidine-4-yl) octahydropyrrolo [3, 4-c] pyrrole, and the structural formula of the pyrimidine heterocyclic compound is shown in the specification. Researches find that the pyrimidine heterocyclic compound shows remarkable insecticidal activity on plant parasitic nematodes such as meloidogyne incognita and heterodera glycines, and when the effective concentration is 10 mu g / mL, the death rate of the meloidogyne incognita and the death rate of the heterodera glycines all exceed 50% within 24 h; when the effective concentration is 30 [mu] g / mL, the death rates of the meloidogyne incognita and the soybean cyst nematode can reach 100%.

The invention discloses a soybean cyst nematode No.5 physiological race resistance associated SCN2 molecular marker, a primer and application, and relates to the technical field of gene engineering. The soybean cyst nematode No.5 physiological race resistance associated SCN2 molecular marker is located at a soybean second chromosome 35309928 bp position, specific primers of the molecular marker comprise two forward primers and a reverse primer, and the kit contains the specific primers of the SCN2 molecular marker. The invention relates to an application of an SCN2 molecular marker in breeding of a soybean cyst nematode No.5 physiological race. The invention aims to solve the technical problems of scarcity of disease-resistant varieties, tedious traditional identification process and lack of specific molecular markers in existing soybean anti-soybean cyst nematode fifth physiological race breeding. According to the technical scheme, the KASP molecular marker directly associated with resistance, a matched specific detection primer combination and a rapid detection and application method are included, early-stage, high-throughput and accurate identification of the soybean anti-soybean cyst nematode 5 physiological race character is achieved, and the disease-resistant breeding efficiency is remarkably improved.

The application discloses a protein resisting soybean cyst nematode disease and an encoding gene and application thereof, and belongs to the technical field of plant disease resistance, and solves the technical problem of how to resist soybean cyst nematode disease. The application provides a protein resisting soybean cyst nematode disease, the sequence of the protein is shown as SEQ ID NO. 8, the encoding gene of the protein is shown as SEQ ID NO. 7, and the gene is named GmC2H2-2like. GmC2H2-2like is a gene with the effect of resisting soybean cyst nematode, and overexpression of the gene in soybean can significantly improve the resistance of a root system of a receptor soybean germplasm to cyst nematodes, thereby providing an effective molecular marker and gene resource for molecular design breeding of soybean cyst nematode resistance, and having important theoretical significance and practical value for realizing transgenic breeding of cyst nematode-resistant strains, accelerating the process of pest-resistant breeding and improving the breeding efficiency.

The application discloses application of a pyrimidine heterocyclic compound in plant parasitic nematode control and belongs to the technical field of plant parasitic nematode control; the pyrimidine heterocyclic compound is 2-(5-bromopyrimidine-2-yl)-5-(6-ethyl-5-fluoropyrimidine-4-yl) octahydropyrrolo[3,4-c]pyrrole; research finds that the pyrimidine heterocyclic compound shows significant insecticidal activity on plant parasitic nematodes such as southern root-knot nematode and soybean cyst nematode; when the effective concentration is 10 μg / mL, the mortality of the southern root-knot nematode and the soybean cyst nematode both exceeds 50% in 24 h; when the effective concentration is 30 μg / mL, the mortality of the southern root-knot nematode and the soybean cyst nematode both can reach 100%.

The invention discloses a soybean cyst nematode population dynamic monitoring method and system based on the Internet of Things, and the method comprises the steps: collecting insect pest detection data and monitoring data in a gene-driven insect pest prevention and control preset region, and carrying out the preprocessing of the insect pest detection data and the monitoring data; performing target screening on the pest detection data to obtain target detection data, and performing prevention and control risk assessment on the target detection data based on the monitoring data to obtain a prevention and control ratio; performing causal analysis on the pest detection data according to gene driving to obtain an ecological risk degree, and constructing an optimized growth degree function according to the control ratio and the ecological risk degree; and constructing an Internet of Things soybean cyst nematode population dynamic monitoring model according to the optimized growth degree function, inputting to-be-monitored data into the Internet of Things soybean cyst nematode population dynamic monitoring model, and outputting a monitoring result. The method improves the precision of dynamic monitoring of the nematode population, and can be directly applied to a dynamic monitoring system of the nematode population.