79 results about "Heterodera" patented technology

A soybean cultivar designated BA922834 with high yield potential, tolerance to Roundup™ herbicide, MG VI maturity, resistance to race 3 of soybean cyst nematode (Heterodera glycines), and resistance to frogeye leaf spot, further including the plants and seeds of the cultivar BA922834, methods for producing a soybean plant by crossing the cultivar BA922834 with itself or another soybean plant. The invention also relates to soybean cultivar BA922834 further comprising one or more single gene traits, and to methods of producing a soybean having such traits by introgression, transformation or mutagenesis. The invention also includes using the soybean cultivar BA922834 to produce other soybean cultivars, breeding lines, and progeny.

The invention which provides a Chinese herbal medicinal pesticide and a usage method thereof relates to the pesticide field. The Chinese herbal medicinal seed dressing pesticide is prepared by decocting 470-550g of Radix Euphorbiae Ebractealatae, 460-520g of Stemona sessilifolia, and 460-530g of Sophora flavescens in 18000-25000g of water for 1-3h. The Chinese herbal medicinal seed dressing pesticide is used according to a case that each 1kg of the Chinese herbal medicinal seed dressing pesticide is uniformly stirred with 100-170kg of crop seeds, and then the crops seeds are sown. The Chineseherbal medicinal pesticide of the invention, which has the advantages of reasonable compatibility, low cost and simple preparation method, allows problems of high residual, high pollution and bad pest prevention effect of traditional chemical pesticides to be solved, and simultaneously has control effects on root rot diseases and Heterodera glycines of crops of soybean and the like, and pests of soil grubs, cutworms, borers and the like.

The invention discloses a nematicidal composition and application thereof. The composition comprises active ingredients A and B, and a mass ratio of A to B is 20:1 to 1:20. The ingredient A refers to one of fosthiazate, abamectin, furfural, dazomet, fluopyram, carbosulfan, metham, verticillium chlamydosporium, emamectin benzoate, paecilomyces lilacinus or fluensulfone; and the structural formula of the ingredient B is a formula I or a formula II, wherein the formula I is as shown in the specification, and the formula II is as shown in the specification. The nematicidal composition disclosed by the invention is used for killing plant parasite nematodes comprising root-knot nematodes, ditylenchus destructor, heterodera sachachtii, pratylenchus coffeae and bursaphelenchus xylophilus, is composed of a novel nematicide containing lactonic rings and the existing insecticides and bactericides, and has good nematocidal activity and low toxicity to people and livestock. The nematicidal composition disclosed by the invention has an excellent effect of controlling eggs and second-stage larvae of the root-knot nematodes.

The invention relates to a method for cultivating a fungus capable of poisoning and killing plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The Trichoderma strain Snef85 involved in the present invention is Trichoderma harzianum, which has been preserved on October 30, 2007 in the General Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing, postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the Trichoderma strain Snef85 is Deuteromycotina, Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and Trichoderma harzianum. It is prepared by liquid fermentation culture, and its metabolites have high toxicity to plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean cyst nematodes, and outstanding nematicidal effect. The fermentation broth of the strain has obvious control effects on root-knot nematode (Meloidogynes pp.), rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines). There are significant differences between the treatment of fermented liquid with different dilution concentrations and the treatment of aseptic water, and the nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.

The invention relates to a natural plant biological composite nutrient solution for killing nematode and a preparation method thereof. The natural plant biological composite nutrient solution for killing nematode comprises bacillus subtilis bacillus subtilis, paecilomyces lilacinus, beauveria bassiana and plants marigold flower, stemona, maidenhair, sophora flavescens, derris trifoliata, melia azedarach and protein liquid according to weight ratio, compared with prior art, the natural plant biological composite nutrient solution has beneficial effects that harm due to soil borne parasites such as root-knot nematode, heterodera sachachtii, and ditylenchus dipsaci can be effectively prevented and treated; no pesticide residue on soil and crops fruits can be realized, usage is convenient, no pollution is generated, nematode harm can be avoided by using the solution for three times in a whole fertility period, and the nutrient solution creates condition for producing green organic food; and has good prevention and treatment effects for crop soil borne disease, can improve the soil and increase soil granular structure, and is capable of preventing soil hardening, increasing the content of the organic matter, dissolving phosphor, releasing potassium and fixating nitrogen, as well as supplementing crop nutrition.

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention relates to a method for identifying resistance against heterodera glycines. The method comprises the following steps: scissoring the tissue of young part of the soybean vegetative part of the susceptible control variety and the variety to be identified to perform inductive rooting culture; after the transplanted seedling survives, inoculating the seedling with heterodera glycines suspension containing 400 eggs and/or second-stage larvae/mL; continuing culture at 20-30 DEG C; when white cyst appears at the seedling root, separating counting the number of root cyst of the susceptible control variety and the variety to be identified; and defining the disease resistance of the material according to the resistance grading standard. The method provided by the invention has the positive and beneficial effects that: as the genotype is not changed when the vegetative body roots, the resistance of the vegetative body can represent the resistance of the parent material; one part of the vegetative body of the material to be identified replaces the entire plant for identification, and the resistive material can be completely stored; and for the population of segregative generation (for example, F2), 3-5 branches on one individual plant can be fetched for identification so as to realize repeated identification of the segregation population.

The invention discloses lecanicillium attenuatum and application of the lecanicillium attenuatum to controlling heterodera glycines. The lecanicillium attenuatum is lecanicillium attenuatum HNLA1306, which is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number of CGMCC No.9220. A fermentation liquor of the lecanicillium attenuatum can remarkably restrain incubation of eggs of heterodera glycines and has a strong killing effect on J2 larvas; the conidia of the lecanicillium attenuatum have a good parasitic effect on second-stage juveniles of bemisia tabaci; the lecanicillium attenuatum can be used for biological control of crop nematodes and bemisia tabaci.

The subject invention concerns plants protected from nematode feeding damage and improved versions of Cry proteins. The subject invention also concerns improved versions of Cry6A proteins. Synthetic genes encoding these modified proteins are also part of the subject invention. Another embodiment of the subject invention includes plants transformed with the genes of the subject invention. In yet another embodiment the subject invention concerns Bt proteins for in-plant protection against crop damage by root knot nematode (RKN; Meloidogyne incognita) and soybean cyst nematode (SCN; Heterodera glycines).

The invention discloses an insecticidal composition containing an organophosphorus compound. In an insecticidal composition containing an active ingredient A and an active ingredient B, the active ingredient A is selected from lythidathion; the active ingredient B is any one selected from the following compounds: chlorpyrifos, profenofos, triazophos, malathion and phoxim; and the weight ratio of the active ingredient A to the active ingredient B is 1:80-80:1. By using the composition disclosed by the invention, multiple pests can be controlled; obvious synergistic action is obtained; the insecticidal spectrum is expanded; higher activities are obtained for grubs, mole crickets, heterodera, cotton bollworms, pink bollworms, rice planthoppers, Cnaphalocrocis medinalis, striped rice borers, rice water weevils, beet armyworms, aphids, armyworms, budworms and the like on vegetables, rice, cottons, corns, wheat, fruit trees and soybeans; the pesticide usage is reduced; the residual quantity of the pesticide on the crops is reduced; and the environment pollution is reduced; and the insecticidal composition containing the organophosphorus compound, disclosed by the invention, is safe for people and livestock, shows excellent environment compatibility and is not easy for the pests to generate resistance.

Soybean cyst nematode and soybean sudden death syndrome resistance genes, soybean cyst nematode and soybean sudden death syndrome resistant plant lines, and methods of breeding and engineering same.

The invention belongs to the technical field of microbial pesticides and particularly relates to bacillus thuringiensis for controlling heterodera glycines and meloidogyne incognita as well as a preparation and applications. The bacillus thuringiensis HAN055 having high toxicity for the heterodera glycines and meloidogyne incognita is obtained through separation. The preparation WP-HAN055 preparedfrom the strain has significant control effects on heterodera glycines and meloidogyne incognita damage. The bacillus thuringiensis HAN055 is collected in CCTCC (China Center for Type Culture Collection) with the collection number of CCTCC NO: M2015608.

The invention relates to application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis, and belongs to the field of biological prevention and treatment. The tea saponin serves as a main effective component, is high in toxic activity to heterodera glycines, and can be effectively used for preventing and treating the soybean sporocyst nematodiasis.

The invention relates to a pest-killing and disease-resistant suspended seed coating agent. The seed coating agent is prepared by processing active ingredients and non-active ingredients, wherein the active ingredients comprise cyantraniliprole, difenoconazole and a plant growth regulation agent; the non-active ingredients comprise a wetting dispersant, a film forming agent, a microelement fertilizer, a stress-resistant agent, an anti-freezing agent, a preservative, a defoaming agent, a thickening agent, a coloring agent and the like. The suspended seed coating agent has a good pesticide effect, a persistent effect and high work efficiency, is economical and safe and is mainly used for controlling wheat powdery mildew, wheat aphid, cotton fusarium wilt, cotton aphid, a maize take-all disease, thrips, soybean root rot, heterodera glycines ichinohe and other kinds of pest and disease damage.

The invention relates to a culture medthod for Streptomyces zaomyceticus Snea182 capable of inducing heterodera glycines resistance of soybeans after soybean seeds are treated with the strain and application of the strain. The actinomycetes strain Snea182 provided by the invention is already preserved in the China General Microbiological Culture Collection Center. After the strain is subjected tofermentation culture with a common liquid, a seed treatment agent for treating soybean seeds can be developed from metabolite of the strain, and the resistance of soybeans of the soybean seeds treatedwith the strain to heterodera glycines can be induced. After the soybean seeds are treated with the Streptomyces zaomyceticus Snea182 metabolite, remarkable resistance of soybeans to a first generation of heterodera glycines in a seedling period can be induced, a remarkable inhibition function on formation of heterodera glycines cytocyst is achieved, the strain is a potential biocontrol bacterium, and a novel idea for preventing and treating heterodera glycines diseases is developed.

The invention discloses a method for aided detection of heterodera glycines resistance and special primers for detection of heterodera glycines resistance. A primer pair for detecting the resistance of the heterodera glycines to be tested is provided and is composed of a single-stranded DNA molecule shown in sequence 1 in a sequence table and a single-stranded DNA shown in sequence 2 in the sequence table. The experiment provided by the invention shows that the primers designed by the invention can be used for specifically identifying the race 3 physiological race soybean of the heterodera glycines with high sensitivity and high accuracy, the selecting efficiency of the material in the breeding process can be greatly increased and the breeding material can be screened.

The present invention is one kind of microbe and its preparation process. The microbe is amphibious Monacrosporium janus strain SGM-1 with preservation number of CGMCC No. 1036 used in biological prevention and control of plant Sclerotinia sclerotiorum and Heterodera glycines disease. The strain exhibits relatively strong parasitizing capacity on both Sclerotinia sclerotiorum and Heterodera glycines. Through inoculation with the antagonistic microbe, expanding culture and liquid fermentation, corresponding microbe slurry may be obtained; and the microbe slurry may be mixed with diatomite as carrier to prepare bacteria preparation for biological prevention and control of plant Sclerotinia sclerotiorum and Heterodera glycines disease.

The invention discloses bacterium with an induced resistance activity and a cultural method and application thereof, wherein the bacterium is enterobacter cloacae and the preservation number of the enterobacter cloacae is CGMCC No. 10801. The bacterium with an induced resistance activity or the metabolite thereof can be used for preparation of a soybean seed treating agent and biological prevention and control of plant parasitic nematodes. The enterobacter cloacae (E.cloacae) Sneb572 or the metabolite thereof has an activity of inducing soybeans, can be used for soybean seed treatment, therefore not only is the growth of soybean plants and roots promoted, but also a great inhibiting effect on formation of heterodera glycines cysts is achieved, and the problem of prevention and control of heterodera glycines is solved.

The invention provides a method for screening a reference gene in the real-time quantitative PCR analysis of a heterodera glycines infected wild soybean root tissue, and relates to the fields of biology and genetics. A high-resistance high-sensitive wild soybean germplasm is taken as a test material, the condition of gene expression of 24 candidate housekeeping genes in a wild soybean root system in different heterodera glycines infection periods is detected, the expression abundance and the expression stability are evaluated so as to screen the reference gene suitable for studying the expression of the gene in the heterodera glycines infected wild soybean root system, and the method is used for exploring the resistance mechanism of the wild soybean to the heterodera.

The invention relates to the technical field of prevention and treatment of plant diseases, in particular to application of benzoic acid in prevention and treatment of plant nematode diseases, wherein the benzoic acid is used as an effective component in nematicide. Based on the research result of the invention, the benzoic acid has strong toxic activity on various nematodes such as Meloidogyne, Heterodera, Bursaphelenchusxylophilus and the like, can be used for effectively preventing and treating plant nematode diseases, has the advantages of low harm to a human body, low toxicity, low residue, easiness in degradation, greenness, environmental protection and the like, and has good application values are realized in the aspects of preventing and controlling the plant nematodes and developing related medicament products.