Method for aided detection of heterodera glycines resistance and special primers for detection of heterodera glycines resistance

A soybean cyst nematode, resistance technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of difficult success, long time, etc., achieve high accuracy, improve selection High efficiency and high sensitivity
CN104774937AInactive Publication Date: 2015-07-15INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Publication Date
2015-07-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for aided detection of heterodera glycines resistance and special primers for detection of heterodera glycines resistance. A primer pair for detecting the resistance of the heterodera glycines to be tested is provided and is composed of a single-stranded DNA molecule shown in sequence 1 in a sequence table and a single-stranded DNA shown in sequence 2 in the sequence table. The experiment provided by the invention shows that the primers designed by the invention can be used for specifically identifying the race 3 physiological race soybean of the heterodera glycines with high sensitivity and high accuracy, the selecting efficiency of the material in the breeding process can be greatly increased and the breeding material can be screened.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for assisting detection of soybean cyst nematode resistance and special primers. Background technique

[0002] Soybean cyst nematode (soybean cyst nematode, SCN) is an important disease that endangers the production of soybean (Glycine max L.Merr) in the world. Cost-effective measures against soybean cyst nematode disease. Due to the genetic complexity of SCN resistance genes and the diversity of soybean cyst nematode races (Riggs and Schmidt 1988; Caviness 1992), it takes a long time to develop varieties resistant to soybean cyst nematode disease by traditional breeding methods. Time, and difficult to succeed, can not meet the current development of line-resistant breeding, an urgent need for a more accurate and efficient method of identifying SCN. With the development and use of molecular markers, molecular marker-assisted selection (Molecular marker-assisted selection, ...

Claims

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