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Rice variety 9194 black streaked dwarf restricting locus and molecular marking method thereof

A black-streaked dwarf disease and molecular marker technology, applied in the field of molecular genetics, can solve problems such as unsatisfactory control effects, environmental pollution, and destruction of ecological balance, and achieve the effects of convenient identification, clear location, and high difficulty

Active Publication Date: 2013-06-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no specific pesticides for this kind of disease in production. The main method is to reduce the harm of SBPH in the early stage, but the control effect is not ideal, and it pollutes the environment and destroys the ecological balance.

Method used

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  • Rice variety 9194 black streaked dwarf restricting locus and molecular marking method thereof
  • Rice variety 9194 black streaked dwarf restricting locus and molecular marking method thereof
  • Rice variety 9194 black streaked dwarf restricting locus and molecular marking method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] Materials and Methods:

[0031] (1) 9194 / Su Yunuo F 2 Population construction and phenotyping

[0032] (1) The black-streaked dwarf resistance variety 9194 was used as the female parent, and the black-streaked dwarf disease-susceptible japonica rice variety Su Yunuo was used as the male parent to prepare hybrids to construct 9194 / Su Yunuo F 2 separate groups, each F 2 A single plant obtained the corresponding 9194 / Su Yunuo F by selfing 2:3 family lineage.

[0033] (2) Phenotype identification

[0034] (2.1) Field induction identification

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Abstract

The invention relates to a rice variety 9194 black streaked dwarf restricting locus and a molecular marking method thereof. Four rice variety 9194 black streaked dwarf restricting locus are obtained by carrying out genetic linkage analysis on the black streaked dwarf resistance level of the genotype of an F2 single plant obtained from hybridization of the black streaked dwarf restricting rice variety 9194 and susceptible variety suyunuo and the corresponding F2:3 family, wherein qRBSDV3 is positioned between the markers RM282 and RM14898, qRBSDV6 is positioned between the markers RM20069 and RM30, qRBSDV9 is positioned between the markers RM3912 and RM257, and qRBSDV11 is positioned between the markers RM26062 and RM536. The molecular markers of the black streaked dwarf restricting gene are used for detecting whether the resistant variety 9194 and the derived varieties contain the black streaked dwarf restricting locus, so that the resistance level of the black streaked dwarf can be predicated, and the selecting efficiency of the black streaked dwarf restricting rice is greatly improved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a rice variety 9194 resistant to black-streaked dwarf locus and a molecular marker method thereof. technical background [0002] Rice is an important food crop in my country. Rice black-streaked dwarf disease is a viral disease caused by rice black-streaked dwarf virus (RBSDV), which is mainly transmitted by persistent non-ovipositous means by the small planthopper. In recent years, with changes in farming systems and cultivation methods, warming of the winter climate, and large-scale promotion of susceptible varieties, the incidence of the virus-transmitting agent SBPH has increased. It occurred on a large scale in Fujian, and quickly rose to become the most important viral disease in production, posing a great threat to the safe production of rice. The typical symptoms of rice black-streaked dwarf disease are dwarf diseased plants, dark green and stiff leaves, early waxy white...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13
Inventor 万建民刘裕强江玲孙志广胡金龙王宝祥王琦赵志刚王益华陈亮明刘世家刘喜陈赛华张文伟田云录王春明徐大勇
Owner NANJING AGRICULTURAL UNIVERSITY
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