32 results about "Soyedina" patented technology

The invention discloses a molecular marker for identifying a soybean variety resistant to cyst nematode disease and application, and belongs to the technical field of molecular marker-assisted breeding. The invention aims to more quickly and accurately screen the soybean variety resistant to cyst nematode disease. The invention provides a molecular marker for identifying a soybean variety resistant to cyst nematode disease. The 8382716 site in a Glyma. 19G051500 gene is G or C; the 846511th site in the Glyma. 19G051900 gene is A or G, and the 8466511th site in the Glyma. 19G051900 gene The 8522589 site in the Glyma. 19G052400 gene is A or C; the 8481473 site in the Glyma. 19G052100 gene is T or C; or the 181 site in the sequence as shown in SEQ ID NO.4 is T or C. And favorable reference basis and technical support are provided for soybean cyst nematode disease-resistant molecule assisted breeding.

Provided are plants, cells, tissues, and germplasm thereof comprising gene and marker alleles associated with increased resistance to soybean cyst nematode (SCN). Also provided are breeding methods and methods of identifying and selecting plants having alleles associated with increased resistance to SCN. Transgenic and genome edited plants comprising the gene alleles associated with increased SCN resistance are provided.

Compositions and methods for conferring nematicidal activity upon bacteria, plants, plant cells, tissues, and seeds are provided. Methods for killing or controlling populations of nematode pests, such as cyst nematode species, for example, *Heterodera glycines* (soybean cyst nematode), root-knot nematodes, kidney-shaped nematodes, short-bodied nematode species, or lanceolate nematode populations, are also provided. These methods further include contacting the nematode pest with a pest-killing amount of a polypeptide containing a nematicidal toxin. Further methods include increasing plant yield by expressing genes of the embodiments in plants.

The invention relates to the technical field of plant molecular breeding and molecular biology, in particular to a KASP molecular marker related to soybean mosaic virus resistance and application of the KASP molecular marker. The molecular marker is targeted to an anti-soybean mosaic virus specific functional SNP (Single Nucleotide Polymorphism) site of a soybean GmLMM4 gene; the SNP locus is c.743Cgt of a soybean GmLMM4 gene coding region, and the SNP locus is a nucleotide sequence of the soybean GmLMM4 gene; the GmLMM4 gene is mutated by a T mononucleotide, and the 248th amino acid of the GmLMM4 gene coding protein is changed into isoleucine from threonine through the mutation. The molecular marking tool provided by the invention has the advantages that the accuracy of anti-SMV breeding can be remarkably improved, the matched primer group is designed in a targeted antisense strand, and the C / C, C / T and T / T genotypes can be accurately distinguished by FAM / HEX fluorescence signals and correspond to soybean anti-SMV phenotypes; high-throughput detection is completed in the seedling stage, the screening efficiency of high-resistance materials is improved, the identification period is shortened, and the screening efficiency of soybean anti-SMV breeding is improved.

The invention discloses application of a KASP molecular marker in preparation of a preparation for identifying soybean mosaic virus, and belongs to the technical field of molecular assisted breeding. According to the application of the KASP molecular marker in preparing the preparation for identifying the soybean mosaic virus disease, the KASP molecular marker is located at the 30473748bp position of the No.13 chromosome of a soybean reference genome, and the basic group is G / A. The molecular marker is closely linked with the soybean mosaic virus disease, and can be used for preliminarily screening soybean mosaic virus disease resistant varieties, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean mosaic virus resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant allelic genes provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The invention discloses application of soybean GmVQ70 to improvement of soybean cyst nematode resistance, relates to the technical field of genetic engineering, and solves the problems that existing chemical control pollutes the environment and is difficult to implement crop rotation, and traditional breeding is long in period and prone to loss of resistance. The method comprises the following steps: 1, constructing an overexpression vector of the GmVQ70 gene to obtain a transformed strain; and 2, infecting soybean plant tissues by the transformed strain. The invention proves that the soybean GmVQ70 gene has the effect of regulating and controlling soybean cyst nematode resistance of soybeans for the first time. A receptor soybean plant is subjected to genetic transformation by using an overexpression vector of the GmVQ70 gene, and after soybean cyst nematode infection, the number of soybean root surface female insects can be remarkably reduced by 38.4% compared with a wild type, so that the resistance of soybeans to soybean cyst nematode is improved, and a gene resource is provided for soybean resistance breeding. The application and the method of the GmVQ70 gene play a key role in improving the resistance process of soybean cyst nematode, and a new molecular target can be provided for soybean precise molecular design breeding.

The invention belongs to the technical field of plant molecular breeding, and discloses a molecular marker related to soybean cyst nematode disease resistance and application thereof. The CAPS molecular marker provided by the invention is simple to match and operate, and can quickly distinguish three genotypes of susceptibility, disease resistance and excellent phenotype and disease resistance and scabs. The molecular marker does not need expensive detection equipment, is simple and convenient to operate, low in cost and accurate in typing, is suitable for screening soybean cyst nematode-resistant filial generations, identifying transgenic lines and detecting the purity of disease-resistant varieties, can remarkably improve the breeding efficiency and shorten the breeding period, and provides a practical technical tool for soybean cyst nematode resistance breeding.

The invention discloses a complex microbial inoculant for preventing and treating carya illinoensis perwing moths and application of the complex microbial inoculant, and belongs to the technical field of pest prevention and treatment. The complex microbial inoculant for preventing and treating the carya illinoensis pernicifuge is prepared from the following raw materials in parts by mass: 2 to 3 parts of beauveria bassiana bacterial liquid, 3 to 5 parts of bacillus thuringiensis bacterial liquid, 4 to 8 parts of brevibacillus laterosporus bacterial liquid, 5 to 6 parts of bacillus cereus bacterial liquid, 8 to 10 parts of sodium alginate, 3 to 5 parts of seaweed extract, 1 to 2 parts of liquorice root extract and 3 to 4 parts of soybean extract. The complex microbial inoculant disclosed by the invention has an excellent prevention and treatment effect on the perfond moth, and the problems that the existing chemical agent is easy to generate drug resistance and pesticide residue, destroys ecological balance, threatens food safety and human health and the like are solved.

Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Further provided are methods for killing or controlling a population of nematode pests, in particular Pratylenchus species, the present invention relates to a population of nematodes, such as Pratylenchus brachycaeus, Pratylenchus punctatus, Pratylenchus schriberi, Pratylenchus neglectus, Pratylenchus zeae, Blonolaimus longicaudatus, Hoplolaimus galeae, Helicida dihysteria, Helicida pseudomonas, or Xiphinea americana, a root-knot nematode, a reniform nematode, a soybean cyst nematode, or a spear nematode population, for example, a population of nematodes, such as Pratylenchus brachycaeus, Pratylenchus sp., Pratylenchus sp. The methods comprise contacting the nematode pest with a pesticidally effective amount of a polypeptide comprising a nematicidal toxin. Further included are methods of increasing plant yield by expressing a gene of an embodiment in a plant.

Provided are plants, cells, tissues, and germplasm thereof comprising gene and marker alleles associated with increased resistance to soybean cyst nematode (SCN). Also provided are breeding methods and methods of identifying and selecting plants having alleles associated with increased resistance to SCN. Transgenic and genome edited plants comprising the gene alleles associated with increased SCN resistance are provided.

The application discloses application of a Glyma.04G199400 gene in regulation of soybean cyst nematode resistance and belongs to the technical field of genetic engineering. The CDS sequence of the Glyma.04G199400 gene is shown as SEQ ID NO. 3. The application mines the gene Glyma.04G199400 which regulates soybean cyst nematode 3 physiological race resistance. It is confirmed that overexpression of the gene can significantly reduce the cyst number on soybean hairy roots and improve the soybean cyst nematode resistance, thereby providing theoretical support for soybean disease resistance mechanism research and providing new gene resources and technical support for soybean trait improvement and high-resistance soybean breeding.

This invention belongs to the field of agricultural pest control technology, specifically relating to the application of reducing the expression level of the pathogenic effector gene RPCYP1 of the variegata stink bug in the control of soybean bud blight. This invention discovered and identified a key pathogenic effector gene of the variegata stink bug, RPCYP1, and demonstrated that specifically silencing this gene can effectively block the ability of the variegata stink bug to induce bud blight symptoms in soybeans. Simultaneously, this invention also provides a method for controlling soybean bud blight caused by the variegata stink bug, which includes the steps of delivering specific double-stranded RNA that reduces the expression level of the RPCYP1 gene into the variegata stink bug or spraying it onto the surface of soybean plant tissue. This invention intervenes in the pathogenic process of the pest at the molecular level, providing a novel technical approach for the control of soybean bud blight.

The invention discloses a primer group for detecting a KASP molecular marker and application of the primer group in preparation of a preparation for identifying soybean cyst nematode disease, and belongs to the technical field of molecular assisted breeding. The primer group for detecting the KASP molecular marker disclosed by the invention is as shown in SEQ ID NO. 3 to SEQ ID NO. 5. The KASP molecular marker is located at the 8361148bp position of the No.8 chromosome of the soybean reference genome, and the basic group is C / G. The molecular marker is closely linked with soybean cyst nematode disease, and can be used for preliminary screening of soybean cyst nematode disease resistance, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean cyst nematode resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant alleles provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The invention discloses application of a KASP molecular marker in preparation of a preparation for identifying soybean cyst nematode disease, and belongs to the technical field of molecular assisted breeding. According to the application of the KASP molecular marker in preparing the preparation for identifying the soybean cyst nematode disease, the KASP molecular marker is located at the 32969916bp position of the 11th chromosome of a soybean reference genome, and the basic group is C / A. The molecular marker is closely linked with soybean cyst nematode disease, and can be used for preliminary screening of soybean cyst nematode disease resistance, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean cyst nematode resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant alleles provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The invention provides a combined module for enhancing symbiotic nitrogen fixation capacity of rhizobium and application, and belongs to the technical field of synthetic biology. The combined module for enhancing the symbiotic nitrogen fixation capacity of the rhizobium comprises an inducible promoter element Pi and a transcriptional regulatory factor coding gene tetR, wherein the inducible promoter element Pi and the transcriptional regulatory factor coding gene tetR are sequentially connected; the inducible promoter element Pi specifically responds to a symbiotic nitrogen fixation signal; according to the invention, the combined module is inserted into an initial vector to construct a recombinant expression vector, and the recombinant expression vector is transferred into different soybean rhizobium chassis through a triparental conjugation method to obtain two nitrogen fixation recombinant engineering strains; the recombinant engineering strain can recover the influence of glyphosate stress on the symbiotic nitrogen fixation capability of the rhizobium.

The invention provides a soybean cyst nematode identification tracking method and system based on a time sequence image, and relates to the technical field of time sequence identification, and the method comprises the steps: collecting a continuous time sequence microscopic image of a root sample through a microscopic imaging device, carrying out the enhancement standardization of a preprocessing model, executing a multi-scale adaptive segmentation algorithm, and constructing a spatial-temporal feature map, and feature similarity and spatial displacement constraints between adjacent frames are calculated based on a Monte Carlo tree search method, and accurate identification and dynamic tracking of the soybean cyst nematode are realized. The soybean cyst nematode identification accuracy and tracking efficiency are improved, and a scientific basis is provided for crop pest control.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance, as well as methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenic and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

This invention discloses a Bacillus velezensis strain B05 and its application in controlling soybean root rot. The Bacillus velezensis strain B05 has the accession number CCTCC NO: M 2026071. Strain B05 significantly inhibits the growth of Fusarium and other pathogens. This invention is the first to screen specific genes associated with the superior antibacterial phenotype of strain B05 and clarify their sequence characteristics. Compared with other Bacillus velezensis strains, these specific genes increase the inhibition rate of strain B05 against Fusarium to over 90%, providing targeted genes for functional optimization of Bacillus velezensis and reducing the blindness of genetic engineering.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a molecular marker for identifying southern root knot nematode disease resistance and application of the molecular marker. The molecular marker provided by the invention targets a functional site c.1075A / C of a soybean nematode-resistant key gene GmLMM1, and the site variation leads to the change of encoded amino acid from asparagine to histidine. The KASP primer group designed based on the site can quickly and accurately identify a disease-resistant C / C homozygous type, a susceptible A / A homozygous type and an A / C heterozygous type. According to the molecular marker tool provided by the invention, the accuracy of meloidogyne incognita resistant breeding can be remarkably improved, the screening efficiency of resistant materials is improved, the breeding period is effectively shortened, and an efficient and reliable technical means is provided for soybean meloidogyne incognita resistant breeding.

The invention discloses application of a Glyma. 04G199400 gene in regulation and control of soybean cyst nematode resistance, and belongs to the technical field of gene engineering. The CDS sequence of the Glyma. 04G199400 gene is as shown in SEQ ID NO. 3, and the CDS sequence is as shown in SEQ ID NO. The gene Glyma. 04G199400 for regulating and controlling the resistance of the physiological race 3 of the soybean cyst nematode is excavated, it is proved that overexpression of the gene can remarkably reduce the number of cysts on hairy roots of soybeans, the resistance of the soybean cyst nematode is improved, theoretical support is provided for research of a soybean disease-resistant mechanism, and the gene Glyma. And new gene resources and technical support are provided for soybean character improvement and high-resistance soybean breeding.

The invention discloses application of thuringirin in prevention and treatment of cotton aphid, green peach aphid, armyworm and soybean cyst nematode, and belongs to the field of microbial pesticides. The invention discovers that the thurinin has the biological activity of killing cotton aphid, green peach aphid, mythimna separata and soybean cyst nematode for the first time, has a wide application prospect, and can be developed into a novel environment-friendly biological control agent.

Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Further, methods for killing or controlling a nematode pest population, particularly a Pratylenchus spp., e.g., Pratylenchus brachyurus, Pratylenchus penetrans, Pratylenchus scribneri, Pratylenchus neglectus, Pratylenchus zeae, Belonolaimus longicaudatus, Hoplolaimus galeatus, Helicotylenchus dihystera, Helicotylenchus pseudorobustus, or Xiphinema Americanum, root knot nematode, reniform nematode, soybean cyst nematode or Lance nematode population, are provided. The methods include contacting the nematode pest with a pesticidally-effective amount of a polypeptide comprising a nematicidal toxin. Further included are methods for increasing yield in plants by expressing the genes of the embodiments in plants.

This invention provides a combined module and its application for enhancing the symbiotic nitrogen fixation ability of rhizobia, belonging to the field of synthetic biology. The combined module for enhancing the symbiotic nitrogen fixation ability of rhizobia includes sequentially connected inducible promoter elements Pi that specifically respond to symbiotic nitrogen fixation signals and elements from... Sinorhizobium Fred transcriptional regulatory factor encoding genes tetR The present invention inserts the combined module into the initial vector to construct a recombinant expression vector, and then uses the triparental conjugation method to transfer the recombinant expression vector into different soybean rhizobium chassis to obtain two nitrogen-fixing recombinant engineered strains. Compared with wild-type rhizobium, the recombinant engineered strains of the present invention can restore the effect of glyphosate stress on the symbiotic nitrogen-fixing ability of rhizobium.

The invention belongs to the technical field of prevention and control of agricultural pests, and particularly relates to an apis cerana sialoprotein RP426 with HAMP activity and application of the apis cerana sialoprotein RP426. The sialoprotein RP426 secreted into the soybean during feeding of the bee bugs is identified through a mass spectrometry technology for the first time, and further research proves that the RP426 is a novel HAMP molecule and can activate PTI reaction of plants, induce early ROS outbreak of the plants and defend up-regulated expression of related genes, so that the resistance of the plants to pathogenic bacteria and phytophagous pests is enhanced. According to the present invention, with the application of the Emblycoris punctata sialoprotein RP426, the defects of the existing prodenia litura prevention and control measures are made up, the use of the chemical pesticide is reduced, and the new approach is provided for improving the resistance of the plant to the pathogenic bacteria and the resistance of the plant to the phytophagous pests.

This invention belongs to the field of biotechnology, specifically involving GmNIC1 Genes and GmNIC2 The application of genes in regulating the ability of plants to resist soybean cyst nematode, the aforementioned GmNIC1 The nucleotide sequence of the gene is shown in SEQ ID NO.1; GmNIC2 The nucleotide sequence of the gene is shown in SEQ ID NO.2. This invention discloses for the first time... GmNIC1 as well as GmNIC2 The negative regulatory role of genes in soybean resistance to cyst nematode (SCN), and through construction GmNIC1 and GmNIC2 The study identified gene silencing mutants and verified that the loss of function in this gene family significantly enhances soybean resistance to SCN. This application provides a new target for a deeper understanding of the soybean-nematode interaction mechanism and offers novel gene resources and technical pathways for molecular design breeding of resistant soybeans.

This invention relates to a soybean cyst nematode gene, Hg-tax-4, and its application in nematode control. This invention addresses the problems of environmental pollution and easy loss of resistance in existing chemical control methods for soybean cyst nematodes, particularly when combined with resistant varieties and non-host rotation methods. The nucleotide sequence of the soybean cyst nematode gene Hg-tax-4 is shown in SEQ ID NO: 1 in the sequence listing. The application of the dsRNA of the soybean cyst nematode gene Hg-tax-4 in nematode control is also discussed. The gene Hg-tax-4 is derived from the cyclic nucleotide-gated ion channel subunit of the soybean cyst nematode, and experimental results show that this gene is expressed in the cerci of second-instar larvae. The soybean cyst nematode gene Hg-tax-4 participates in regulating the infection and reproduction of the soybean cyst nematode on its host and can be used as a target gene for the development of drugs for the control of soybean cyst nematodes.

This invention discloses GmEXO70 Application of genes in enhancing soybean resistance to nematodes. This invention is the first to discover and validate a gene encoding the EXORDIUM protein through single-cell transcriptome analysis. GmEXO70 It is specifically and highly expressed in the syncytia of disease-resistant soybean varieties, and its expression is strongly induced by nematode infection. Overexpression was confirmed by constructing an overexpression vector and transforming soybean hairy roots with Agrobacterium rhizogenes. GmEXO70 It can significantly increase the expression level of this gene in roots. Further resistance evaluation showed that, compared with the empty vector control, overexpression significantly increased the expression level of this gene. GmEXO70 In the root system, the proportion of nematodes developing to the J3 / J4 stage was significantly reduced, with most nematodes arresting at the J2 stage. Furthermore, immunofluorescence in situ hybridization directly confirmed at the transcriptional level that... GmEXO70 The gene was specifically enriched in nematode-induced syncytia. This invention provides a new key gene for soybean nematode resistance breeding. GmEXO70 .

The invention discloses application of a GmPP2C-37like gene in improving soybean cyst nematode disease resistance of soybeans, and belongs to the technical field of plant disease resistance breeding. The invention aims to improve the soybean cyst nematode disease resistance of soybeans. The invention provides an application of a GmPP2C-37like gene in improving the capability of soybeans for resisting soybean cyst nematode disease. A nucleic acid small marker of the GmPP2C-37like gene is shown as SEQ ID NO.7. The invention further provides a preparation method of the GmPP2C-37like gene. The invention focuses on a new approach of resisting soybean cyst nematode disease, and excavates a new gene resource for improving soybean variety resistance.

The application of soybean gene GmSnRK2C-e in plant cadmium stress resistance belongs to the technical field of plant genetic engineering. The application of the soybean gene GmSnRK2C-e in plant cadmium stress resistance is provided to solve the technical problems that the cadmium tolerance of the soybean quality is poor in the prior art, and the research process of improving the cadmium tolerance of soybean by traditional breeding methods is slow, the nucleotide sequence of the soybean gene GmSnRK2C-e is shown as SEQ ID NO. 1, and the application of the expression vector and the recombinant bacteria containing the above-mentioned gene in plant cadmium stress resistance. The wild type and overexpression transgenic plants under cadmium stress are observed, it is found that compared with the wild type, the overexpression transgenic plants are less inhibited in growth, the shortening of the plant roots is lighter, and the cell damage of the plant leaves is smaller; the results prove that the soybean gene GmSnRK2C-e has the ability of positively regulating the cadmium stress resistance of soybean plants, and the provided GmSnRK2C-e gene has important theoretical significance and practical value for accelerating the breeding process of cadmium-resistant plants and improving the breeding efficiency.

The application discloses application of a soybean C2H2 type zinc finger transcription factor gene GmRGR1. The application of the soybean C2H2 type zinc finger transcription factor gene GmRGR1 shown in SEQ ID NO. 1 in genetic engineering modification of a chlorophyll content of Arabidopsis thaliana. Overexpression of the GmRGR1 gene can reduce the chlorophyll content of Arabidopsis thaliana. The soybean C2H2 type zinc finger transcription factor gene GmRGR1 can be transformed into Arabidopsis thaliana through genetic engineering, and finally negatively regulates the chlorophyll content of Arabidopsis thaliana.