31 results about "Soyedina" patented technology

The invention discloses molecular markers of soybean mosaic virus resistant gene (RSC8) and applications thereof. The invention discloses two pairs of novel primers for screening resistant gene (RSC8) related with soybean anti-soybean mosaic virus in the soybean molecular breeding field, a preparation method of molecular markers and applications. The analysis results show that the provided molecular markers (ZL-39 and ZL-52) can precisely trace the resistant gene (RSC8) of a soybean disease-resistant species (Kefeng No.1), and can predict the resistance of soybean to soybean mosaic virus, so that researchers can more conveniently identify and screen anti-soybean mosaic virus materials under the laboratory conditions. The molecular marker assisted breeding technology can avoid the influences of environmental factors and artificial factors on phenotype; and the provided two pairs of molecular markers (ZL-39 and ZL-52), which are tightly interlocked with the resistant gene (RSC8), can improve the accuracy in selection of anti-soybean mosaic virus breeding materials so as to achieve the goals of breeding soybean species which can resist soybean mosaic virus.

The invention relates to entomogenous fungus Nomuraearileyi, as well as a culture method and an application thereof. The collection number of the Nomuraearileyi Nr1001 is CGMCCNO. 6387. A microbial agent is formed by mixing a Nomuraearileyi Nr1001 fermentation solution, a surfactant and an anti-corrosion agent. A screened Nr1001 strain has the advantages of broad insecticidal spectrum and good insecticidal effect and stronger dissemination against lepidoptera noctuidae insects, such as larvae of cotton bollworm, asparagus caterpillar, prodenia litura, three-spotted plusia, soybean looper, oriental tobacco budworm and the like, and can prevent the prevalence of field pests and plant diseases, effectively reduce the population density of the pests and realize a better pest control effect.

The invention discloses a soybean cyst nematode Hg-flp-1 gene as well as an encoding protein and application thereof, and relates to a soybean cyst nematode neuropeptide gene as well as an encoding protein and application thereof. The invention aims to solve the problem of environmental pollution in the existing chemical control method of soybean cyst nematode. The nucleotide sequence of the geneis shown as SEQ ID NO: 1 in a sequence table. The amino acid sequence of the encoded protein is shown as SEQ ID NO: 2 in a sequence table. After the neuropeptide gene Hg-flp-1 is subjected to dsRNA treatment, the number of second-stage instar larvae in infected roots and the number of root surface female insects after 21 days of infection are reduced by 25% and 28.9% respectively. The soybean cystnematode Hg-flp-1 gene disclosed by the invention is applied to the field of soybean cyst nematode prevention and control.

The invention relates to a culturing method for a fungus having plant nematode poisoning activity and a preparation method of a metabolite of the fungus, belonging to the technical field of microbial pesticides. A fungus strain Snef5 related to the invention is gloeotinia nematicida which is collected in the China General Microbiological Culture Collection Center (Address: Datun Road, Chaoyang District, Beijing, Post Code: 100101) with the collection number CGMCC No.4549 on January 24, 2011. The taxonomic statuses of the fungus strain Snef5 are ascomycota, ascomycetes, helotiales and sclerotiniaceae, and gloeotinia belongs to a fungus. The fungus is prepared by performing liquid fermentation culturing. A metabolite of the fungus has very high toxicity on major pathogen nematodes in plant nematodes such as root-knot nematodes, soybean cyst nematodes and aphelenchoides besseyi, a remarkable nematode killing effect and good application and development prospects.

The invention provides an SMV (soybean mosaic virus)-resistant gene GmST1, a cultivation method of GmST1 transgenic soybeans and application, and belongs to the technical field of soybean genetic breeding. Aiming at solving the problems of polyploidization and low conversion rate of cultivation of disease-resistant varieties with a traditional method, the cultivation method of the SMV-resistant transgenic soybeans is provided and comprises steps as follows: the SMV-resistant transgenic soybean gene GmST1 with the nucleotide sequence represented as SEQ ID NO:1 is constructed to a plant expression vector, and a soybean gene GmST1 recombinant vector is obtained; the recombinant vector is introduced into agrobacterium and infects the soybeans, and after cultivation, a transgenic positive plantis obtained by identification. The SMV-resistant gene GmST1 and the cultivation method can be applied to SMV-resistant crop breeding work.

The invention discloses a soybean GmEF1B gene mutant plant as well as a preparation method and application thereof, and belongs to the technical field of plant biology. The soybean GmEF1B gene mutantplant is provided to clarify the disease-resistant function of soybean genes resistant to sclerotinia sclerotiorum and accelerate the breeding process of soybean varieties resistant to sclerotinia sclerotiorum, and contains a mutant soybean GmEF1B gene. The mutation means base substitution or deletion occurring to a 5'-GCTTCCCCGGCAATGCTCAA-3' nucleotide sequence in a GmEF1B gene coding region so as to change the coded amino acid sequence, and the mutant plant is prepared by a CRISPR / Cas9 gene editing method, and can be used for breeding soybean varieties resistant to sclerotinia sclerotiorum.

The invention discloses a method for aided detection of heterodera glycines resistance and special primers for detection of heterodera glycines resistance. A primer pair for detecting the resistance of the heterodera glycines to be tested is provided and is composed of a single-stranded DNA molecule shown in sequence 1 in a sequence table and a single-stranded DNA shown in sequence 2 in the sequence table. The experiment provided by the invention shows that the primers designed by the invention can be used for specifically identifying the race 3 physiological race soybean of the heterodera glycines with high sensitivity and high accuracy, the selecting efficiency of the material in the breeding process can be greatly increased and the breeding material can be screened.

This invention relates to a recombinant deoxyribonucleic acid (DNA) which can be isolated from Aspergillus soyae, characterised in that it codes for a leucine aminopeptidase (LAP) and comprises a nucleotide sequence corresponding to the nucleotide sequence given in SEQ ID NO: 1 for the mature LAP or to a nucleotide sequence derived therefrom which hybridises under stringent conditions with the nucleotide sequence given in SEQ ID NO: 1 for the mature LAP. The invention further relates to vectors and transformed host organisms, and also relates to methods of producing LAP. Enzyme products for the production of protein hydrolysates, as well as protein hydrolysates which are produced correspondingly, also form part of the invention.

The invention provides a molecular marker ZCindel246000 coseparated from a soybean epidemic disease resisting gene RpsZS18, and an application thereof. The marker is positioned between the SSR markers ZCSSR33 and ZCSSR46 of a soybean No.2 chromosome, and has a genetic distance of 0cM to the gene RpsZS18. A new SSR marker is exploited by using a soybean SSR marker in an interval and using a soybean genome sequence according to a preliminary positioning result with an F3 column generated through hybridizing and selfing disease resisting variety Zaoshu 18 used as a male parent and a susceptible variety Williams used as a female parent as a mapping colony, and a disease resisting gene contained in the Zaoshu 18 is finely positioned. The disease resisting gene RpsZS18 is positioned between the ZCSSR33 (0.7cM) and ZCSSR46 (2.8cM) of the No.2 chromosome, and is coseparated from an SSR marker Satt172. An RpsZS18 candidate gene is predicated according to gene note in the new positioning interval, the Indel marker ZCindel24600 is exploited according to the candidate gene site sequence difference predicated by resistant and susceptible parents, a colony verification result shows that the marker is a coseparated marker, and the Indel marker can be used in auxiliary breeding of soybean epidemic disease resistance.

The invention discloses a molecular marker Insert 144 closely chained with a soybean epidemic-disease-resistant gene RpsQ. A nucleotide sequence of the molecular marker Insert 144 is shown as SEQ ID No.1, and a genetic distance between the molecular maker and the soybean epidemic-disease-resistant gene RpsQ is 0 cM. Detection analysis shows that the molecular marker can accurately track the soybean epidemic-disease-resistant gene RpsQ and predict epidemic-disease-resistant characteristics of soybean, so that convenience is brought to molecular design breeding. The invention further discloses a method for identifying the soybean epidemic-disease-resistant gene RpsQ. By utilizing the method, quick identifying detection of soybean epidemic-disease-resistant plants can be realized.

The invention belongs to the technical field of soybean molecular breeding, and particularly relates to molecular markers and primers for detecting a soybean phytophthora root rot resistance gene, a detection method and application of the molecular markers and primers. The primers are a primer pair composed of upstream and downstream primers of a molecular marker sat186 and a molecular marker satt683 in the sequence table. The invention provides the soybean phytophthora root rot resistance genes molecular marker sat186 and molecular marker satt683, the linkage distances are 5.1 cM and 9.3 cM respectively, and the disease resistance gene Rps1498 has disease resistance to an ultrastrong phytophthora sojae strain PsJS2. The molecular markers closely linked to the disease resistance gene can be used for performing marker-assisted selection on the soybean phytophthora root rot resistance gene to prolong the disease resistance of the variety. The soybean phytophthora root rot resistance gene molecular markers and the special primers thereof can play an important role in soybean disease resistance breeding.

The invention provides an application of trichoderma in antagonizing soybean quarantine diseases. The invention relates to the technical field of biocontrol agents. In the embodiment, trichoderma harzianum, trichoderma viride and trichoderma spiroides are separated and utilized; bactericidal tests on Diaporthe phaseolorum caulivora and Phomopsis longicolla are carried out; it is shown by test results that trichoderma harzianum, trichoderma viride and trichoderma spirale have good antibacterial effects and antagonistic effects on Diaporthe phaseolorum caulivora and Phomopsis longicolla, and canbe used as standard strains for biological prevention and treatment.

The invention relates to a PCR identification method of soybean rhizobium strain 5873, and primers and application of the method, and belongs to the technical field of strain identification. The method comprises the following steps: using the liquid after lysis, boiling water bath and freeze-thaw of the genomic DNA of strains to be tested or pure culture liquid of the strains or a root nodule crushed extract as templates, and performing PCR amplification using primers 4-4; performing electrophoresis of the PCR products, and identifying that the samples without bands around 355 bp are not the soybean rhizobium strain 5873; continuing PCR amplification of the strains to be test by using a primer Q1, when bands around 355 bp exist; and performing electrophoresis of the above products, and identifying that the samples with bands around 218 bp are the soybean rhizobium strain 5873. According to the present invention, the method has the following advantages that the soybean rhizobium strain5873 can be distinguished simply and quickly in terms of strain identification and evaluation, and bacterial bodies or root nodules can be directly used as templates, so that the tedious steps such asseparation, culture and DNA extraction and identification root nodules are omitted.

The invention discloses a soybean cyst nematode RNA helicase CGH-1, and a coding gene and application thereof. The protein disclosed by the invention is (a) or (b) as shown below: (a) a protein composed of an amino acid sequence shown in sequence 1 in a sequence list; and (b) a protein derived from sequence 1, which is formed by the substitution and / or deletion and / or addition of one or more amino acid residues in the amino acid sequence shown in sequence 1 and is related to the growth of soybean cyst nematodes. In the invention, the soybean cyst nematode RNA helicase CGH-1 and the coding gene thereof are provided, and an RNAi expression vector is designed on the basis of the coding gene; and the RNAi expression vector can be introduced into a plant, possibly the dsRNA of the gene in the plant, so that the nematodes can suffer from severe reproduction or growth dysfunction after taking in the transgenic plant, thereby inhibiting the infection and spread of the nematodes. By using the method disclosed by the invention, a transgenic plant having very high nematode resistance activity can be obtained, thereby laying a foundation for the further cultivation of new broad-spectrum nematode-resistant soybean varieties.

The subject invention concerns plants protected from nematode feeding damage and improved versions of Cry proteins. The subject invention also concerns improved versions of Cry12A proteins. Synthetic genes encoding these modified proteins are also part of the subject invention. Another embodiment of the subject invention includes plants transformed with the genes of the subject invention. In yet another embodiment the subject invention concerns Bt proteins for in-plant protection against crop damage by root knot nematode (RKN; Meloidogyne incognita) and soybean cyst nematode (SCN; Heterodera glycines).

The invention provides a soybean aphid-resistant gene as well as a molecular marker and application thereof, and relates to the technical field of molecular marker-assisted breeding. The soybean aphid-resistant gene is located at the physical positions of 30,700,000-30,900,000,000 on the No.13 chromosome of a soybean genome, and a molecular marker and a corresponding molecular marker detection method are designed based on the soybean aphid-resistant gene. The molecular markers are used for auxiliary selection, so that the screening efficiency is greatly improved, and the breeding period of breeding aphid-resistant varieties is shortened.

The invention relates to a method for identifying soybean cyst nematode resistance. The method is to first cut the tender parts of the soybean vegetative tissues of the susceptible control variety and the variety to be identified to induce rooting culture, and after transplanting and surviving, the seedlings are inoculated with 400 / mL cysts containing eggs and / or second-instar larvae Continue culturing the nematode suspension at 20-30°C. When white cysts appear in the roots of the seedlings, count the number of cysts in the roots of the susceptible control variety and the variety to be identified, and define the disease resistance of the material according to the resistance grading standard. The invention has positive and beneficial effects: the rooting of the vegetative body does not change the genotype, therefore, the resistance of the vegetative body can represent the resistance of the parent material; a part of the vegetative body of the material to be identified is used to replace the whole plant for identification, and the resistant material can be identified Completely preserved; for the group of segregated generations (such as F2), 3 to 5 branches can be taken from a single plant for identification, so as to achieve repeated identification of the segregated population.

The invention discloses a CAPS marker detection method and a primer relevant to soybean cyst nematode resistance. The invention provides application of single nucleotide polymorphism of following SNP locus in a soybean genome or a substance for detecting the single nucleotide polymorphism of the following SNP locus in the soybean genome for identifying or assisting identification of soybean resistance to the soybean cyst nematode; the SNP locus in the soybean genome corresponds to 113th site of nucleotide sequence shown as sequence 1 in a sequence list; and nucleotide at the SNP locus is G or T. The CAPS marker detection method and the primer relevant to the soybean cyst nematode resistance can select materials resistant to the soybean cyst nematode by detecting genotype of the SNP locus, can avoid time-consuming and labor-consuming phenotypic identification, can carry out soybean cyst nematode resistance screening on breeding materials through the genotype, increase selection efficiency of resistant varieties in a breeding process and shorten the breeding time largely, and improve cultivation effects of varieties resisting the soybean cyst nematode significantly.

The invention relates to a PCR identification method for soybean rhizobium strain 5873, primers used therefor and applications, belonging to the technical field of bacterial strain identification. The method comprises: using the genomic DNA of the bacterial strain to be tested or the pure culture bacterial liquid of the bacterial strain or the root nodule crushing extract after cracking, boiling water bath, and freeze-thawed liquid as a template, and performing PCR amplification with primer 4-4; Perform electrophoresis on the PCR product, and if there is no band of about 355bp, it is not soybean rhizobium strain 5873; if there is a band of about 355bp, continue to use primer Q1 for PCR amplification of the strain to be tested; perform electrophoresis on the above product, and there is a band of about 218bp Soybean Rhizobium strain 5873. The present invention has the following advantages: In terms of strain identification and evaluation, soybean rhizobia strain 5873 can be easily and quickly distinguished, and bacteria or nodules can be directly used as templates, eliminating the cumbersome separation, cultivation, and DNA extraction and identification of nodules. link.

The invention provides a purification method and application of a soybean cyst nematode new race X12. The purification method comprises the steps that a soybean cyst nematode group identified as a soybean cyst nematode X12 physiological race is subjected to continuous single-cyst propagation on domestic excellent resistance-source Chrysochelon in Xing County (numbering: ZDD2315) for 4 generations by adopting a single-cyst propagation method, and is continuously purified and propagated on the Chrysochelon in Xing County for 3-4; and the first generation is recovered by using the susceptible variety Lee to realize population purification and propagation of the cysts. The purification method can be used for separating and purifying the X12 race with superstrong pathogenicity from soybean cyst nematode groups mixed in nature, the purified X12 microspecies can be continuously used for genome sequencing research, soybean cyst nematode disease-causing gene research, soybean resistance resource screening, soybean cyst nematode-resistant gene positioning and phenotype identification screening research in the soybean disease-resistant variety cultivation process in multiple generations, and has important application value in related research of soybean cyst nematode.

The invention discloses a CAPS marker detection method and a primer relevant to soybean cyst nematode resistance. The invention provides application of single nucleotide polymorphism of following SNP locus in a soybean genome or a substance for detecting the single nucleotide polymorphism of the following SNP locus in the soybean genome for identifying or assisting identification of soybean resistance to the soybean cyst nematode; the SNP locus in the soybean genome corresponds to 113th site of nucleotide sequence shown as sequence 1 in a sequence list; and nucleotide at the SNP locus is G or T. The CAPS marker detection method and the primer relevant to the soybean cyst nematode resistance can select materials resistant to the soybean cyst nematode by detecting genotype of the SNP locus, can avoid time-consuming and labor-consuming phenotypic identification, can carry out soybean cyst nematode resistance screening on breeding materials through the genotype, increase selection efficiency of resistant varieties in a breeding process and shorten the breeding time largely, and improve cultivation effects of varieties resisting the soybean cyst nematode significantly.

The invention relates to the field of plant genetic engineering, and provides a soybean borer LgPGRP‑LB gene, the base sequence of which is shown in SEQ ID NO:1. The dsRNA of the soybean borer LgPGRP‑LB gene was synthesized in vitro, and the dsRNA of the soybean borer LgPGRP‑LB gene was introduced into the soybean borer larvae at the end of the first instar by feeding method. Gene silencing thus affected the growth and development of soybean borer larvae, and even caused soybean borer larvae to pupate about ten months earlier. The gene can be used as a target gene to construct an RNAi expression vector for cultivating soybean germplasm with high resistance to the soybean borer.

The invention discloses an effective identification method for soybean diseases and insect pests, and solves the problems of hysteresis and inaccuracy in the existing soybean disease and insect pest identification and identification aspects based on field visual observation, laboratory analysis and the like in the prior art. The method comprises the following steps that a database is established by utilizing known soybean plant characteristic spectrums under the influence of different types of soybean diseases and insect pests, and various identification parameters involved in soybean plants are extracted and stored under the influence of each type of disease and insect pests in the database in different growth periods; different identification characteristic parameters are endowed with different weights according to the difference of disease and pest types and the difference of the influence of the disease and pest types on plants, and a discrimination model of the disease and pest types is established according to extraction results of the characteristic parameters; and the discrimination model calculation result of the characteristic parameters of the soybean plant to be identified and the discrimination model calculation result of the plant under each disease and insect pest are compared, calculated and matched, so that the identification and identification of the soybean disease and insect pest type are realized. The method has the advantages of real-time performance and quasi-real-time performance.

The invention provides an application of trichoderma in antagonizing soybean quarantine diseases. The invention relates to the technical field of biocontrol agents. In the embodiment, trichoderma harzianum, trichoderma viride and trichoderma spiroides are separated and utilized; bactericidal tests on Diaporthe phaseolorum caulivora and Phomopsis longicolla are carried out; it is shown by test results that trichoderma harzianum, trichoderma viride and trichoderma spirale have good antibacterial effects and antagonistic effects on Diaporthe phaseolorum caulivora and Phomopsis longicolla, and canbe used as standard strains for biological prevention and treatment.

The invention discloses a Trichogramma domestication breeding method suitable for the prevention and control of soybean borer and its control application. The pine caterpillar Trichogramma and the armyworm Trichogramma co-parasitize the tussah silkworm eggs, and the Trichogramma armyworm controls the tussah silkworm eggs. Trichogramma pine caterpillar can bite through tussah silkworm egg shells to form beehive holes, so that more Trichogramma armyworm individuals can emerge and continue to produce offspring on tussah silkworm eggs. Trichogramma armyworm can Maintaining a relatively stable population enables multi-generation continuous domestication, and gradually optimizes the Trichogramma strains with higher adaptability to tussah eggs, thereby realizing the independent and continuous breeding of Trichogramma on tussah eggs , which laid a technical foundation for the large-scale breeding of the armyworm Trichogramma with tussah eggs. The armyworm Trichogramma bred by the method of the invention has higher parasitic efficiency on the eggs of the soybean borer, and is especially suitable for the biological control of the soybean borer.

The invention belongs to the technical field of agricultural microbiology and relates to application of 3-methylthiopropionic acid in preparation of nematicidal preparations. It is found that the 3-methylthiopropionic acid has good insecticidal activity on nematodes through determination of the fatality rates of meloidogyne incognita, meloidogyne javanica, soybean cyst nematodes and caenorhabditis elegans; The LC50 values of the 3-methylthiopropionic acid on the meloidogyne incognita, meloidogyne javanica and soybean cyst nematode are 3.34 [mu] g / mL, 3.56 [mu] g / mL and 4.93 [mu] g / mL respectively; and after the 3-methylthiopropionic acid is compounded witha compound preparation is compounded with methylmalonic acid, the minimum LC50 of the compound preparation on the meloidogyne incognita is 2.50 [mu] g / mL. The preparation provides a new resource for research and development of a novel plant nematode killing preparation.

The invention relates to the technical field of agricultural microbiology, and in particular, relates to an application of propionamide in preparation of nematicidal preparations, andthrough determination of the fatality rates of meloidogyne incognita, meloidogyne javanica and soybean cyst nematodes, it is found that the propionamide has good insecticidal activity on nematodes, the LC50 values of the propionamide on M.incognita, M.javanica and heterodera glycines are 22.87 [mu]g / mL, 107.02 [mu]g / mL and 46.09 [mu]g / mL respectively, after the propionamide is compounded with betaine, the lowest LC50 value of the compound preparation on the M.incognita is 13.92 [mu]g / mL, and after the compound is compounded with methylmalonic acid, the lowest LC50 value of the compound preparation on the M.incognita is 14.48 [mu]g / mL. The preparations provide a new resource for research and development of a novel plant nematode killing preparation.

The invention discloses a gene for improving cyst nematode resistance of soybeans and application of the gene, and belongs to the technical field of genetic breeding. Aiming at the problems that: thenumber of cyst nematode female insects in infected soybean plants is large, conventional disease-resistant operation difficulty is high at present, and the technical cost is high, the invention provides the gene for improving cyst nematode resistance of soybeans. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1. An Agrobacterium rhizogenes K599 mediated genetic transformation methodis adopted, pCSXN1250-GmRSCN16-1 recombinant plasmid is transferred into an infected Dongnong 50 plant, rooting is induced, physiological races of soybean cyst nematode No.1, No.3 and No.4 are inoculated in a mixed manner, and the invention proves that the GmRSCN16-1 gene has obvious resistance to the soybean cyst nematode. The gene can be used for breeding soybean varieties with the cyst nematode resistance.