27 results about "Soybean mosaic virus" patented technology

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

GsRPP13L The gene, its recombinant vector, recombinant bacteria and application belong to the technical field of plant disease resistance. In order to solve the technical problems that the utilization of SMV resistance genes and the analysis of disease resistance mechanism are not comprehensive enough in the prior art, a broad-spectrum and durable SMV-resistant soybean variety cannot be quickly and efficiently bred, and the resistance variety is prone to "resistance failure", a soybean gene for improving resistance to soybean mosaic virus is provided GsRPP13L The gene is shown in the nucleotide sequence SEQ ID NO.1, and the gene can improve the resistance of tobacco or soybean to soybean mosaic virus through experiments, and the present application provides a theoretical basis for plant resistance to soybean mosaic virus.

The invention discloses an application of a soybean GmHB6 protein or an HB6 gene for coding the protein in antivirus of plants. According to the invention, the GmHB6 gene is introduced into a target plant through an agrobacterium transformation method, so that a soybean GmHB6 gene overexpression plant is obtained. The soybean GmHB6 overexpression plant obtained by the method disclosed by the invention can obviously reduce the infection of soybean mosaic virus.

The invention relates to the technical field of plant molecular breeding and molecular biology, in particular to a KASP molecular marker related to soybean mosaic virus resistance and application of the KASP molecular marker. The molecular marker is targeted to an anti-soybean mosaic virus specific functional SNP (Single Nucleotide Polymorphism) site of a soybean GmLMM4 gene; the SNP locus is c.743Cgt of a soybean GmLMM4 gene coding region, and the SNP locus is a nucleotide sequence of the soybean GmLMM4 gene; the GmLMM4 gene is mutated by a T mononucleotide, and the 248th amino acid of the GmLMM4 gene coding protein is changed into isoleucine from threonine through the mutation. The molecular marking tool provided by the invention has the advantages that the accuracy of anti-SMV breeding can be remarkably improved, the matched primer group is designed in a targeted antisense strand, and the C / C, C / T and T / T genotypes can be accurately distinguished by FAM / HEX fluorescence signals and correspond to soybean anti-SMV phenotypes; high-throughput detection is completed in the seedling stage, the screening efficiency of high-resistance materials is improved, the identification period is shortened, and the screening efficiency of soybean anti-SMV breeding is improved.

The invention relates to the technical field of gene engineering, in particular to a GmOEP16 gene interacting with soybean mosaic virus and application of the GmOEP16 gene, and the GmOEP16 gene is identified as a novel functional gene which interacts with soybean mosaic virus nucleus inclusion proteins NIa-Pro and NIb and can negatively regulate the resistance of soybeans to the soybean mosaic virus. In other words, the soybean mosaic virus resistance of soybeans can be remarkably enhanced by effectively silencing the gene; on one hand, a theoretical foundation is laid for deeply analyzing an interaction molecular mechanism of the GmOEP16-mediated soybean-soybean mosaic virus; on the other hand, an effective gene resource can be provided for breeding a new soybean variety (line) capable of resisting the soybean mosaic virus through a molecular technology.

The invention discloses an application of a soybean B-type response regulatory factor gene GmRR1 in soybean resistance to soybean mosaic virus diseases. The invention also discloses application of the soybean B-type response regulatory factor gene GmRR1 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The over-expression of the gene can significantly improve the resistance of soybeans to soybean mosaic virus diseases. The soybean B-type response regulatory factor gene GmRR1 disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to soybean mosaic virus diseases is finally regulated and controlled.

The invention belongs to the technical field of genetic breeding, and relates to a soybean mosaic virus resistance gene Gm13g184900 as well as a molecular marker and application thereof. According to the invention, the Gm13g184900 gene of a soybean variety Suweon 97 is cloned, the nucleotide sequence and the amino acid sequence of the Gm13g184900 gene are respectively shown as SEQ ID NO.1 and SEQ ID NO.2, and experiments prove that the gene inhibits the proliferation of SMV on a leguminous plant cowpea and reduces the protein accumulation amount of SMV on cowpea leaves. Meanwhile, a soybean plant which stably inherits and expresses the gene is obtained, and it is found that SMV infection can be resisted by expressing the gene on soybeans, so that the gene is indeed an effective anti-SMV gene. The invention also provides a specific molecular marker of the gene, and the molecular marker can specifically identify soybean varieties containing 13g184900R / S allele. The Gm13g184900 gene obtained by the invention can be used for improving the soybean mosaic virus resistance of soybeans, and has a great application prospect in the soybean antiviral breeding industry subsequently.

The invention discloses application of a KASP molecular marker in preparation of a preparation for identifying soybean mosaic virus, and belongs to the technical field of molecular assisted breeding. According to the application of the KASP molecular marker in preparing the preparation for identifying the soybean mosaic virus disease, the KASP molecular marker is located at the 30473748bp position of the No.13 chromosome of a soybean reference genome, and the basic group is G / A. The molecular marker is closely linked with the soybean mosaic virus disease, and can be used for preliminarily screening soybean mosaic virus disease resistant varieties, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean mosaic virus resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant allelic genes provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The invention provides a soybean mosaic virus disease resistant gene GmNAL1 and application thereof, and relates to the technical field of gene engineering. The soybean disease-resistant gene GmNAL1 has a coding region sequence as shown in SEQ ID NO: 1 and an amino acid sequence as shown in SEQ ID NO: 2. The length of the CDS sequence of the gene is 3546bp, and the gene encodes a protein with 1181 amino acids. The protein has an NB-ARC structural domain and an LRR structural domain. The soybean disease-resistant gene GmNAL1 can make up for the deficiency of the existing soybean mosaic virus-resistant gene, and can improve the soybean disease resistance when being applied to soybeans, so that valuable gene resources and theoretical basis can be accumulated for cultivating new soybean mosaic virus-resistant varieties, and the soybean disease-resistant gene GmNAL1 has important application value in soybean disease-resistant gene engineering breeding.

The application belongs to the field of plant genetic engineering, and discloses a GmG3PDH8 gene and application thereof in regulating soybean mosaic virus resistance of soybean, wherein the sequence of the GmG3PDH8 gene is shown as SEQ ID NO. 1, and the GmG3PDH8 gene positively regulates the disease resistance performance of soybean plants to SMV. Based on the disease resistance of the GmG3PDH8 gene, the gene and a recombinant carrier, a transgenic cell or a recombinant bacteria containing the gene can be applied in regulating the soybean mosaic virus resistance of soybean, and can also be applied in cultivating soybean plants resistant to soybean mosaic, thereby laying a good foundation for sustainable development of the soybean industry and providing important gene resources for molecular breeding of soybean to improve the soybean mosaic virus resistance of soybean.

The application discloses application of a soybean GmHB6 protein or an HB6 gene coding the protein in plant antiviral activity. The application introduces the GmHB6 gene into a target plant by a method of agrobacterium transformation, and obtains a soybean GmHB6 gene overexpression plant. The soybean GmHB6 overexpression plant obtained by the method of the application can significantly reduce infection of a soybean mosaic virus.

The invention discloses an application of a soybean cystathionine beta-lyase gene GmCBL2 in soybean resistance to soybean mosaic virus diseases. The invention also discloses application of the soybean cystathionine beta-lyase gene GmCBL2 as shown in SEQ ID NO.1 and SEQ ID NO.2 in genetic engineering modification of soybean mosaic virus resistance. The soybean cystathionine beta-lyase gene GmCBL2 can negatively regulate the resistance of soybeans to diseased soybean mosaic virus diseases, and the antiviral reaction of the soybeans can be activated due to function deficiency of the soybean cystathionine beta-lyase gene GmCBL2. The gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the resistance of soybeans to the soybean mosaic virus disease can be remarkably improved.

The invention discloses an application of a wild soybean GsPC coding gene in soybean mosaic virus resistance. The GsPC gene encodes a plant cyanin, belongs to a blue copper protein family and has typical copper redoxin structure folding, the function of the GsPC gene is speculated to be related to electron transfer, and the nucleotide sequence of the GsPC gene is SEQ ID NO.1. After the SMV-SC7 is used for treating a plant which overexpresses the gene for 21 days, the SMV content in the overexpressed plant is obviously reduced, which indicates that the resistance of the soybean to the soybean mosaic virus disease can be obviously improved by overexpressing the gene. The wild soybean coding gene GsPC disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to the soybean mosaic virus diseases is finally regulated and controlled.

The invention relates to an SNP marker related to soybean mosaic virus resistance and application thereof, and belongs to the field of biology, the SNP marker is located at the 13049396 bp site of a soybean genome No.6 chromosome, and the polymorphism of the SNP marker is C / T. Detecting the polymorphism of the SNP marker related to the soybean mosaic virus resistance in a soybean genome to be detected, and identifying or assisting in identifying the soybean mosaic virus resistance according to the polymorphism of the SNP marker related to the soybean mosaic virus resistance. And detecting the genotype of the SNP marker site at the 13049396 bp site on the No.6 chromosome of the soybean genome, selecting the soybean with the genotype C as a parent for breeding, and harvesting the soybean mosaic virus resistant soybean offspring. According to the method, the resistance of the soybean variety to the soybean mosaic virus can be accurately and quickly identified, technical support is provided for efficiently breeding a high-quality soybean variety with relatively high resistance to the soybean mosaic virus, the breeding time is shortened, and the breeding efficiency is improved.

The invention discloses an application of a soybean S-adenosine-L-methionine methyltransferase gene GmSAMMT in soybean resistance to soybean mosaic virus diseases. The invention also discloses application of the soybean S-adenosine-L-methionine methyltransferase gene GmSAMMT as shown in SEQ ID NO.1 and SEQ ID NO.2 in gene engineering modification of soybean mosaic virus disease resistance. The soybean S-adenosine-L-methionine methyltransferase gene GmSAMMT provided by the invention can negatively regulate the resistance of soybeans to diseased soybean mosaic virus diseases, and the antiviral reaction of the soybeans can be activated due to function deficiency of the soybean S-adenosine-L-methionine methyltransferase gene GmSAMMT. The gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the resistance of soybeans to the soybean mosaic virus disease can be remarkably improved.

The invention discloses a small peptide TaLEP1 and application thereof in antiviral defense of plants, and belongs to the technical field of agricultural biology. The amino acid sequence of the plant antiviral defense small peptide TaLEP1 is shown as SEQ ID NO: 1, and the small peptide TaLEP1 can be used for preparing plant antiviral products, including virus inhibitors and antiviral drugs, and is suitable for inhibiting wheat yellow mosaic virus, turnip mosaic virus and soybean mosaic virus of potyviridae Y. When in use, the TaLEP1 is dissolved in sterile PBS (Phosphate Buffer Solution) to prepare a 5 mu M working solution, the working solution is subjected to exogenous spraying on plant leaves, and after the plant leaves absorb the working solution, the TaLEP1 can induce activation of plant autophagy related pathways, so that virus infection is inhibited, and replication and accumulation of viruses in the plant bodies are reduced. The small peptide TaLEP1 provided by the invention can effectively inhibit the replication of various RNA viruses of potyviridae and has a good antiviral effect.

The invention discloses an application of a soybean acid phosphatase gene GmACP1 in soybean resistance to soybean mosaic virus diseases. The invention relates to an application of a soybean acid phosphatase gene GmACP1 as shown in SEQ ID NO.1 in genetic engineering modification of soybean mosaic virus resistance. The over-expression of the gene can significantly improve the resistance of soybeans to soybean mosaic virus diseases. The soybean acid phosphatase gene GmACP1 disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to soybean mosaic virus diseases is finally regulated and controlled.

The invention discloses a primer group for synchronously detecting seven soybean viruses based on multiple RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and a detection method. The viruses comprise a common kidney bean mosaic virus, a soybean mosaic virus, a light cowpea mottle virus, a soybean green disease virus, a soybean chlorotic leaf roll virus and a pueraria lobata mosaic virus) and a citrus related rhabdovirus. According to the detection method, seven pairs of specific primers (SEQ ID NO.1-14) are included, the cross reaction between the primers is effectively overcome, seven soybean virus fragments can be synchronously amplified in a PCR system, stripes are clear and uniform in distribution, and the detection sensitivity reaches the capacity of independent PCR detection. And after a multiplex PCR amplification system is further optimized, the detection can be economically, quickly and effectively carried out within 2 hours. The method disclosed by the invention has very important significance in monitoring the occurrence condition of soybean virus diseases in the field and predicting disease epidemic.

The invention discloses an application of a soybean lysophosphatidic acid acyltransferase gene GmLPAT4 in soybean resistance to soybean mosaic virus diseases. The invention relates to an application of a soybean lysophosphatidic acid acyltransferase gene GmLPAT4 as shown in SEQ ID NO.1 in genetic engineering modification of soybean mosaic virus resistance. The soybean lysophosphatidic acid acyltransferase gene GmLPAT4 can negatively regulate the resistance of soybeans to diseased soybean mosaic virus diseases, and the antiviral reaction of the soybeans can be activated due to function deficiency of the soybean lysophosphatidic acid acyltransferase gene. The gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the resistance of soybeans to the soybean mosaic virus disease can be remarkably improved.

The invention discloses an SNP molecular marker for soybean mosaic virus resistance-assisted selection, a primer group and application thereof, and relates to the field of biology, the SNP molecular marker is located at the 29327608 bp site of the No.13 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is A / C. The chromosome and the site position of the SNP molecular marker for soybean mosaic virus resistance assisted selection provided by the invention are determined on the basis of a soybean whole genome version number of Glycine max Wm82. A4. V1. The SNP molecular marker and the related primer group obtained by the invention are mainly applicable to development and screening of soybean germplasm resources, and screening and breeding scheme design of soybean germplasm with excellent soybean mosaic virus resistance are realized by performing molecular-assisted identification on soybean mosaic virus resistance.

The invention belongs to the field of genetic breeding and relates to resistance breeding of soybeans. The invention provides a soybean mosaic virus resistant SNP (Single Nucleotide Polymorphism) site, a PARMS primer group and application of the soybean mosaic virus resistant SNP site and the PARMS primer group. The invention discloses SNP (Single Nucleotide Polymorphism) sites with soybean mosaic virus resistance. The SNP sites are T / C mutation at the 46519558 site, G / C mutation at the 47015769 site, A / G mutation at the 48116395 site, G / A mutation at the 49000062 site and G / A mutation at the 50137954 site of a soybean chromosome 9. The invention discloses a PARMS primer group based on an SNP site and application of the PARMS primer group in identifying the mosaic disease resistance of soybeans, and provides an effective technical support for directly identifying susceptible and disease-resistant plants of soybeans and corresponding genotypes, rapidly and accurately identifying the mosaic disease resistance of the soybeans from the genotypes in the early stage of the soybeans and breeding resistant soybeans. The important application value is realized.

The invention discloses an antiviral nano-agent NM4 as well as a preparation method and application thereof, and belongs to the technical field of nano-agent preparation. The anti-virus nano medicament NM4 prepared by the invention has an excellent prevention and treatment effect on plants infected with soybean mosaic viruses, and by applying the medicament NM4 to the plants infected with SMV viruses, the resistance of the soybean plants to SMV infection is obviously enhanced, and the medicament NM4 can inhibit virus accumulation, relieve oxidative stress, protect a cell membrane system and promote photosynthetic metabolism in a synergistic manner, so that the anti-virus nano medicament NM4 has an excellent prevention and treatment effect on the plants infected with the soybean mosaic viruses. Excellent disease-resistant potential and a biological stimulation function are shown. Meanwhile, through multiple mechanisms such as electrostatic adsorption, ROS (reactive oxygen species) regulation, immune activation and energy metabolism maintenance, effective inhibition on SMV infection is jointly realized, and theoretical support is provided for research, development and application of antiviral nano-drugs.

The invention discloses an application of a soybean GmPOT coding gene in resisting soybean mosaic virus diseases. The invention relates to an application of a soybean oligopeptide transporter gene GmPOT as shown in SEQ ID NO.1 in genetic engineering modification of soybean mosaic virus resistance. The over-expression of the gene can significantly improve the resistance of soybeans to soybean mosaic virus diseases. The soybean oligopeptide transporter gene GmPOT disclosed by the invention can be transformed into soybeans through genetic engineering, and the resistance of the soybeans to soybean mosaic virus diseases is finally regulated and controlled.

The application discloses a small peptide TaLEP1 and application thereof in plant antiviral defense, and belongs to the field of agricultural biotechnology. An amino acid sequence of the plant antiviral defense small peptide TaLEP1 is shown as SEQ ID NO:1, and the small peptide TaLEP1 can be used for preparing plant antiviral products, including virus inhibitors and antiviral drugs, and is suitable for inhibiting wheat yellow mosaic virus, turnip mosaic virus and soybean mosaic virus of the Potyviridae family. When used, the TaLEP1 is dissolved in sterile PBS to prepare a 5 muM working solution, and is externally sprayed on plant leaves; after absorption of the plant, the TaLEP1 can induce the activation of a plant autophagy related pathway, and then inhibit virus infection and reduce the replication and accumulation of the virus in the plant. The small peptide TaLEP1 provided by the application can effectively inhibit the replication of various RNA viruses of the Potyviridae family, and has good antiviral effect.

The invention provides a method for identifying soybean mosaic virus (SMV) resistance of genetically improved soybeans obtained by utilizing technologies of gene editing, transgenosis, RNAi interference and the like. The method comprises the following steps: (1) providing a soybean variety to be evaluated; (2) respectively preparing a first solution containing carborundum and a second solution containing soybean mosaic virus, and mixing the first solution and the second solution in proportion to obtain a working solution; (3) spraying the working solution obtained in the step (2) to leaves of a soybean variety to be evaluated by adopting a spray gun inoculation method so as to enable the soybean mosaic virus to intrude into the leaves; and (4) investigating the disease condition after inoculating in the step (3) for a period of time, and identifying the soybean mosaic virus resistance grade of the soybean variety to be evaluated according to the disease performance.

The invention discloses an application of a soybean nuclear transport factor coding gene GmNTF1. The invention also discloses application of the soybean nuclear transport factor gene GmNTF1 shown in SEQ ID NO. 1 in genetic engineering modification of soybean root growth and improvement of soybean yield and mosaic virus resistance. Knockout of the gene can promote root growth and increase soybean yield, and overexpression of the gene can enhance resistance to SMV by inhibiting virus accumulation. The soybean nuclear transport factor gene GmNTF1 disclosed by the invention can be transformed into soybean by utilizing genetic engineering by constructing a gene knockout and overexpression vector, the soybean root development is promoted by knocking out the GmNTF1 gene, the soybean yield is improved, and the resistance of the soybean to soybean mosaic virus diseases can be remarkably improved by highly expressing the GmNTF1 gene.

The invention belongs to the field of plant genetic engineering, and particularly relates to application of a GmROH1 gene in improvement of soybean mosaic virus resistance, and the GmROH1 gene has the accession number of 100806977 in an NCBI library. A key gene GmROH1 related to mosaic virus resistance is screened, it is found that overexpression of the GmROH1 gene in soybeans can improve the mosaic virus resistance of the soybeans, the disease index of soybean plants with the overexpressed GmROH1 gene is moderate resistance, and a new resource is provided for soybean breeding work.