Molecular markers of soybean mosaic virus resistant gene (RSC8) and applications thereof

Abstract

The invention discloses molecular markers of soybean mosaic virus resistant gene (RSC8) and applications thereof. The invention discloses two pairs of novel primers for screening resistant gene (RSC8) related with soybean anti-soybean mosaic virus in the soybean molecular breeding field, a preparation method of molecular markers and applications. The analysis results show that the provided molecular markers (ZL-39 and ZL-52) can precisely trace the resistant gene (RSC8) of a soybean disease-resistant species (Kefeng No.1), and can predict the resistance of soybean to soybean mosaic virus, so that researchers can more conveniently identify and screen anti-soybean mosaic virus materials under the laboratory conditions. The molecular marker assisted breeding technology can avoid the influences of environmental factors and artificial factors on phenotype; and the provided two pairs of molecular markers (ZL-39 and ZL-52), which are tightly interlocked with the resistant gene (RSC8), can improve the accuracy in selection of anti-soybean mosaic virus breeding materials so as to achieve the goals of breeding soybean species which can resist soybean mosaic virus.

Description

technical field [0001] The invention relates to the field of soybean molecular breeding, in particular to a method for identifying and screening soybean mosaic virus resistance gene R SC8 Primer pairs, molecular markers, molecular marker methods and applications. Background technique [0002] Soybean mosaic virus (SMV) disease is a worldwide disease, which occurs in every soybean producing area in the world. It was first reported in Northeast my country in 1899, and then Shandong, Jiangsu, Hubei and other provinces successively reported the harm of the disease. SMV has occurred from north to south in my country. In general, the yield loss caused by SMV is 15-35%, and in some regions, the yield loss can reach more than 70%, or even extinction (Ren et al., 1997). During the long-term co-evolution process of SMV, the host and the environment, pathogenic differentiation occurred, resulting in different pathogenic types, which are called strains. At present, my country uses a ...

AI Technical Summary

Problems solved by technology

However, the use of traditional field or indoor inoculation identification methods for soybean mosaic virus resistance gene R SC8 Tracing offspring is not only time-consuming and labor-intensive, but also has many uncertain factors. The use of molecular marker-assisted selection methods can effectively solve this problem

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