Molecular markers of soybean mosaic virus resistant gene (RSC8) and applications thereof

A soybean mosaic virus and molecular marker technology, which is applied in the directions of application, angiosperm/flowering plant, microorganism determination/inspection, etc., can solve the problems of time-consuming and labor-intensive soybean mosaic virus resistance gene, and achieve stable results, Detect fast effects

Inactive Publication Date: 2015-08-12
CROP INST ANHUI PROV ACAD OF AGRI SCI
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Problems solved by technology

However, the use of traditional field or indoor inoculation identification methods for soybean mosaic virus resistance gene R SC8 Tracing offspring is not only

Method used

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  • Molecular markers of soybean mosaic virus resistant gene (RSC8) and applications thereof
  • Molecular markers of soybean mosaic virus resistant gene (RSC8) and applications thereof
  • Molecular markers of soybean mosaic virus resistant gene (RSC8) and applications thereof

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Embodiment Construction

[0047] 1. Soybean Kefeng 1 anti-SMV gene R SC8 The localization and acquisition of molecular markers

[0048] 1) Materials:

[0049] Disease-resistant variety Kefeng 1: a local soybean variety with broad-spectrum and high resistance to SMV (systematic selection of Hebei local varieties, containing the resistance gene R SC8 ), against most of the currently identified SMV strains, genotype R SC8 R SC8 , the variety has an average growth period of 94 days, a plant height of 53.6 cm, lanceolate leaves, white flowers, brown hairs, limited pod formation habit, convergent plant type, 15.0 nodes on the main stem, and 3.3 effective branches. The height of the bottom pod is 14.1 cm, the number of effective pods per plant is 106.0, the weight of 100 seeds is 7.33 grams, the grain is kidney-shaped, and the seed coat is black. The crude protein content is 43.90%, and the crude fat content is 19.60%.

[0050] Susceptible variety Nannong 1138-2: a variety widely promoted in the middle a...

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Abstract

The invention discloses molecular markers of soybean mosaic virus resistant gene (RSC8) and applications thereof. The invention discloses two pairs of novel primers for screening resistant gene (RSC8) related with soybean anti-soybean mosaic virus in the soybean molecular breeding field, a preparation method of molecular markers and applications. The analysis results show that the provided molecular markers (ZL-39 and ZL-52) can precisely trace the resistant gene (RSC8) of a soybean disease-resistant species (Kefeng No.1), and can predict the resistance of soybean to soybean mosaic virus, so that researchers can more conveniently identify and screen anti-soybean mosaic virus materials under the laboratory conditions. The molecular marker assisted breeding technology can avoid the influences of environmental factors and artificial factors on phenotype; and the provided two pairs of molecular markers (ZL-39 and ZL-52), which are tightly interlocked with the resistant gene (RSC8), can improve the accuracy in selection of anti-soybean mosaic virus breeding materials so as to achieve the goals of breeding soybean species which can resist soybean mosaic virus.

Description

technical field [0001] The invention relates to the field of soybean molecular breeding, in particular to a method for identifying and screening soybean mosaic virus resistance gene R SC8 Primer pairs, molecular markers, molecular marker methods and applications. Background technique [0002] Soybean mosaic virus (SMV) disease is a worldwide disease, which occurs in every soybean producing area in the world. It was first reported in Northeast my country in 1899, and then Shandong, Jiangsu, Hubei and other provinces successively reported the harm of the disease. SMV has occurred from north to south in my country. In general, the yield loss caused by SMV is 15-35%, and in some regions, the yield loss can reach more than 70%, or even extinction (Ren et al., 1997). During the long-term co-evolution process of SMV, the host and the environment, pathogenic differentiation occurred, resulting in different pathogenic types, which are called strains. At present, my country uses a ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68A01H5/00
Inventor 王大刚张磊黄志平李杰坤胡国玉吴倩
Owner CROP INST ANHUI PROV ACAD OF AGRI SCI
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