Soybean antiviral gene and application thereof

A technology of soybean mosaic virus and soybean, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as unsatisfactory variety resistance

Inactive Publication Date: 2013-10-09
NORTHEAST NORMAL UNIVERSITY +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The results of the identification test on mosaic virus disease resistance of new soybean varieties bred in recent years show that: the performance of resistance to infection accounts for about 16% to 27% of the total number of varieties, an

Method used

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  • Soybean antiviral gene and application thereof
  • Soybean antiviral gene and application thereof
  • Soybean antiviral gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Acquisition of disease-resistant transgenic plants

[0050] 1. Identification and cloning of disease resistance genes

[0051] 1. Identification of disease resistance genes:

[0052] (1) Plant material processing and material collection: The soybean variety Rsmv with broad-spectrum and high resistance to soybean mosaic virus disease was used as the resistance gene donor material, and the susceptible soybean variety Ssmv was used as the control material. Sowing, 15 plants of each kind. The growth conditions were 27°C and 18°C ​​for day and night, respectively, and a photoperiod of 17 hours of light and 7 hours of darkness. After the soybean seedlings grow until the second three-edged compound leaf is fully unfolded, inoculate the soybean mosaic virus and the blank control buffer on the first three-edged compound leaf respectively, and wait for the symptoms (yellow spots on the leaves of the susceptible variety) to appear. , and the leaves at different disea...

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Abstract

The invention belongs to the technical field of biology, and relates to a disease resistance detection of a plant antiviral gene and an application thereof. According to the invention, a soybean endogenous antiviral gene Gm-RSMV3 is used to construct a transformation vector which is used for transforming sensitive varieties of soybean mosaic virus; and the transformed plants are subjected to herbicide resistance screening, and then subjected to genome DNA PCR, bar gene expression detection, RT-PCR, Southernblot and inverse PCR, thereby verifying that the obtained plants are indeed transgenic and exogenous genes are correctly expressed. T0 generation plants are selfed to obtain T1 generation plants which are also selfed to obtain T2 generation homozygous plants, and the obtained T2 generation homozygous plants are inoculated with the soybean mosaic virus. A disease resistance testing is carried out and results show that transgenic plants of varieties being originally not resistant to the soybean mosaic virus are relatively high in disease resistance. Conventional cross-breeding and transgenic technologies can be used for enhancing disease-resistance performance of plants and increasing production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the discovery of a soybean antivirus gene, detection of disease resistance and application thereof. Background technique [0002] Soybean mosaic virus disease is a worldwide disease caused by soybean mosaic virus (Potyviridae, Soybean Mosaic Virus, SMV), which seriously affects soybean yield and quality. Soybean mosaic virus belongs to the family Potatoviridae in virus taxonomy, a species of the genus Potatovirus. The members of the Potatovirus genus have in common that the seeds are poisonous, and aphids are the main vectors of virus transmission. Soybean mosaic virus disease is serious, widespread, and difficult to control. Breeding disease-resistant varieties is currently the most effective way to control SMV epidemic. The disease was first discovered in Northeast China in 1899, and was successively found in the United States, North Korea, Germany, the Soviet Union and...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12Q1/68A01H5/00
Inventor 庞劲松寿惠霞贺红利周练刘宝李树珍
Owner NORTHEAST NORMAL UNIVERSITY
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