Soybean antiviral gene and application thereof

Abstract

The invention belongs to the technical field of biology, and relates to a disease resistance detection of a plant antiviral gene and an application thereof. According to the invention, a soybean endogenous antiviral gene Gm-RSMV3 is used to construct a transformation vector which is used for transforming sensitive varieties of soybean mosaic virus; and the transformed plants are subjected to herbicide resistance screening, and then subjected to genome DNA PCR, bar gene expression detection, RT-PCR, Southernblot and inverse PCR, thereby verifying that the obtained plants are indeed transgenic and exogenous genes are correctly expressed. T0 generation plants are selfed to obtain T1 generation plants which are also selfed to obtain T2 generation homozygous plants, and the obtained T2 generation homozygous plants are inoculated with the soybean mosaic virus. A disease resistance testing is carried out and results show that transgenic plants of varieties being originally not resistant to the soybean mosaic virus are relatively high in disease resistance. Conventional cross-breeding and transgenic technologies can be used for enhancing disease-resistance performance of plants and increasing production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the discovery of a soybean antivirus gene, detection of disease resistance and application thereof. Background technique [0002] Soybean mosaic virus disease is a worldwide disease caused by soybean mosaic virus (Potyviridae, Soybean Mosaic Virus, SMV), which seriously affects soybean yield and quality. Soybean mosaic virus belongs to the family Potatoviridae in virus taxonomy, a species of the genus Potatovirus. The members of the Potatovirus genus have in common that the seeds are poisonous, and aphids are the main vectors of virus transmission. Soybean mosaic virus disease is serious, widespread, and difficult to control. Breeding disease-resistant varieties is currently the most effective way to control SMV epidemic. The disease was first discovered in Northeast China in 1899, and was successively found in the United States, North Korea, Germany, the Soviet Union and...

AI Technical Summary

Problems solved by technology

The results of the identification test on mosaic virus disease resistance of new soybean varieties bred in recent years show that: the performance of resistance to infection accounts for about 16% to 27% of the total number of varieties, and the high resistance varieties account for about 15%. General and medium-sensitivity varieties account for one-third, and high-sensitivity varieties account for 33%~40%. It can be seen that the current resistance of varieties is not ideal

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