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SSR marker CH0211 for identifying resistance of soybeans to soybean mosaic virus SC3 strain and detection method and application of SSR marker CH0211

A technology for soybean mosaic virus and detection method, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. The effects of improved disease resistance breeding efficiency, high accuracy and high economic benefits

Active Publication Date: 2020-11-27
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] At present, some molecular markers related to anti-SMV have been screened in China, and some experts also use these markers for auxiliary screening of anti-SMV, but the coincidence rate between the selection of markers and the selection of phenotype is not high, and the material samples used are relatively small

Method used

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  • SSR marker CH0211 for identifying resistance of soybeans to soybean mosaic virus SC3 strain and detection method and application of SSR marker CH0211
  • SSR marker CH0211 for identifying resistance of soybeans to soybean mosaic virus SC3 strain and detection method and application of SSR marker CH0211
  • SSR marker CH0211 for identifying resistance of soybeans to soybean mosaic virus SC3 strain and detection method and application of SSR marker CH0211

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Embodiment 1

[0036] The experimental materials are:

[0037] 288 varieties newly bred by major domestic breeding units in 2018 are preserved in the National Soybean Improvement Center of Nanjing Agricultural University.

[0038] The SSR marker CH0211 primer sequence used is:

[0039] Upstream primer: 5'-CAGGTCGATAAGTCTATGT-3' (SEQ ID NO.1);

[0040] Downstream primer: 5'-ATATGAACTCGGTCTGTTG-3' (SEQ ID NO.2).

[0041] The above labeled primers were used to detect the resistance of soybean varieties to soybean mosaic virus SC3 strain.

[0042] S1. Among the 288 varieties newly bred by major domestic breeding institutions in 2018 preserved by the National Soybean Improvement Center of Nanjing Agricultural University (Table 1), the genomic DNA was extracted according to the operation guide of the plant genomic DNA extraction kit (Kangwei, CW0531M). The SSR marker CH0211 was subjected to PCR amplification. The PCR amplification system was 10 μL, including 5 μL of 2×Taq PlusMasterMix, 0.5 μL of...

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Abstract

The invention discloses an SSR marker CH0211 for identifying the resistance of soybeans to an SMV (Soybean Mosaic Virus) epidemic strain SC3 as well as a detection method and application of the SSR marker CH0211. The SSR marker CH0211 which is newly developed and closely linked with the gene of the anti-soybean mosaic virus SC3 strain is used for carrying out disease resistance selection on a soybean variety so as to obtain a new soybean germplasm of the anti-SMV SC3 strain. According to the method, the defects of long period, large workload, low selection efficiency, susceptibility to environmental influence on selection of disease-resistant phenotypes and the like of conventional soybean mosaic virus-resistant breeding are overcome, the selection efficiency of disease-resistant breedingcan be greatly improved, possibility is provided for direct selection of genotypes, and the disease-resistant breeding process of soybeans is greatly promoted.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to an SSR marker CH0211 for identifying soybean resistant soybean mosaic virus epidemic strain SC3 varieties, a detection method and application thereof. Background technique [0002] Soybean mosaic virus (SMV) disease is one of the most common and most harmful virus diseases in soybean production, which seriously endangers the yield and quality of soybean ( figure 2 ). Traditional breeding for SMV resistance requires manual inoculation to identify the disease resistance of each offspring family, which is time-consuming and laborious, and is easily limited by environmental conditions (especially temperature), which affects the selection of resistant material phenotypes. With the development of modern molecular biology, the advancement of modern biotechnology has provided powerful assistance for crop breeding. Molecular Marker-Assisted Selection (MAS), as one of the i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/13C12Q2600/156C12Q2565/125
Inventor 李凯蔡晗沈颖陈圆圆谢丽君陈柏羽
Owner NANJING AGRICULTURAL UNIVERSITY
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