An ssr marker ch0211 for identifying soybean resistance to soybean mosaic virus sc3 strain and its detection method and application

A technology for soybean mosaic virus and detection method, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. The effect of improving the efficiency of disease resistance breeding, high economic benefits and saving manpower

Active Publication Date: 2022-07-15
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] At present, some molecular markers related to anti-SMV have been screened in China, and some experts also use these markers for auxiliary screening of anti-SMV, but the coincidence rate between the selection of markers and the selection of phenotype is not high, and the material samples used are relatively small

Method used

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  • An ssr marker ch0211 for identifying soybean resistance to soybean mosaic virus sc3 strain and its detection method and application
  • An ssr marker ch0211 for identifying soybean resistance to soybean mosaic virus sc3 strain and its detection method and application
  • An ssr marker ch0211 for identifying soybean resistance to soybean mosaic virus sc3 strain and its detection method and application

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Experimental program
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Embodiment 1

[0036] The experimental materials are:

[0037] There are 288 varieties newly bred by major domestic breeding units in 2018 in the National Soybean Improvement Center of Nanjing Agricultural University.

[0038] The sequence of the SSR marker CH0211 primer used is:

[0039] Upstream primer: 5'-CAGGTCGATAAGTCTATGT-3' (SEQ ID NO.1);

[0040] Downstream primer: 5'-ATATGAACTCGGTCTGTTG-3' (SEQ ID NO. 2).

[0041] The above-marked primers were used to detect the resistance of soybean varieties to soybean mosaic virus SC3 strain.

[0042] S1. Among the 288 varieties newly bred by major domestic breeding units in 2018 stored in the National Soybean Improvement Center of Nanjing Agricultural University (Table 1), the genomic DNA was extracted according to the operation guide of the Plant Genomic DNA Extraction Kit (Kangwei, CW0531M), and the The SSR-labeled CH0211 was amplified by PCR, and the PCR amplification system was 10 μL, including 5 μL of 2×Taq PlusMasterMix, 0.5 μL of the ups...

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Abstract

The invention discloses an SSR marker CH0211 for identifying the resistance of soybean to soybean mosaic virus (Soybean Mosaic Virus, SMV) epidemic strain SC3 and its detection method and application. The closely linked SSR marker CH0211 was used to select soybean varieties for disease resistance, in order to obtain new soybean germplasm resistant to SMV SC3 line. The method avoids the shortcomings of conventional soybean mosaic virus resistance breeding, such as long cycle, large workload, low selection efficiency, and easy environmental impact on disease resistance phenotype selection, can greatly improve disease resistance breeding selection efficiency, and is a direct response to genotypes. Selection provides the possibility, which will greatly promote the process of soybean disease resistance breeding.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to an SSR marker CH0211 for identifying soybean resistant soybean mosaic virus epidemic strain SC3 variety, and a detection method and application thereof. Background technique [0002] Soybean Mosaic Virus (SMV) disease is one of the most common and most serious viral diseases in soybean production, which seriously affects the yield and quality of soybean ( figure 2 ). Traditional SMV-resistant breeding requires artificial inoculation to identify the disease resistance of each progeny family, which is time-consuming and labor-intensive, and is easily limited by conditions such as environment (especially temperature), which affects the selection of resistant material phenotypes. With the development of modern molecular biology, the progress of modern biotechnology has provided a powerful help for crop breeding. Molecular Marker-Assisted Selection (MAS), as one of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/13C12Q2600/156C12Q2565/125
Inventor 李凯蔡晗沈颖陈圆圆谢丽君陈柏羽
Owner NANJING AGRICULTURAL UNIVERSITY
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