85 results about "Cloning vector" patented technology

The present disclosure relates to the seminal discovery of a generation and use of genetically engineered Vibrio sp. Provided is the use of the genetically engineered bacteria for the construction, maintenance, manipulation, and / or propagation of DNA constructs; protein expression; protein secretion; vectors and other metabolic tools; metabolic engineering; expression of cellular extracts for cell-free biology; shuttle vectors; cloning vectors; and for synthetic biology applications. The disclosure also relates to the use of the replication machinery of Vibrio sp. as a cloning or expression vector for replication of recombinant DNA constructs. The disclosure also relates to methods of use of the above.

The invention provides a recombinant salmon mitochondrial gene (mDNA) as well as construction and application of a cloning vector of the recombinant salmon mitochondrial gene (mDNA). After the recombinant salmon mitochondrial gene (mDNA) is subjected to vector construction, cell transformation and proliferation amplification, polydeoxyribonucleotide (PDRN) with higher purity and yield can be prepared on a large scale, and compared with PDRN prepared from wild type mitochondrial DNA, the PDRN prepared from the recombinant mitochondrial gene has the advantages of oxidation resistance, inflammation resistance, improvement of cell proliferation ability, improvement of cell proliferation ability and the like. Compared with the prior art, the PDRN prepared from the salmon testis has remarkable and better effects in the aspects of resisting ultraviolet damage, perfecting the cell repair capacity and the like, the effect is equivalent to that of PDRN prepared from the traditional salmon testis, a new thought or method is provided for large-scale preparation or industrial production of polydeoxyribonucleotide, and the application prospect is wide.

The invention discloses a sugarcane streak mosaic virus infectious cloning vector as well as a construction method and application thereof, and belongs to the technical field of biology. The recombinant plasmid provided by the invention contains a whole genome of the SCSMV, and the nucleotide sequence of the whole genome is the 771 to 10583 nucleotides of SEQ ID No. 1. The recombinant plasmid disclosed by the invention is obtained by taking a pCB301 vector as a skeleton and then inserting the skeleton into a whole genome of SCSMV, and the recombinant agrobacterium tumefaciens (pCB301-SCSMV infectious clone) prepared by the recombinant plasmid can successfully infect nicotiana benthamiana and has efficient infection ability; the infectious clone of the recombinant expression plasmid prepared from the recombinant plasmid can successfully infect nicotiana benthamiana, has efficient infection ability and can express foreign proteins.

Provided are a method for screening a site-directed integration site, a vector microparticle, a site-directed integration method and a microparticle. Provided are a method for screening a site-directed integration site, a vector microparticle, a site-directed integration method and a microparticle. The objective of the present invention lies in that by integrating a marker gene having recognition sequences upstream and downstream on a vector microparticle, protein expression screening and single clone screening are simultaneously performed so as to obtain a single clone vector having good performance by means of one-step screening, and the marker gene is then replaced when an exogenous gene of interest is expressed so as to greatly ensure the overall stability of the microparticle, which ensure the overall expression performance and stability of the expression microparticle while shortening the development of the expression microparticle, thereby solving the technical problem in the prior art that expression vectors are long in development time or poor in performance. The provided method for screening a site-directed integration site can effectively shorten the development period of expression vectors having high expression and overall stability. The provided vector microparticle has good adaptability, good expression and good passaging stability.

The invention discloses a construction method of CiYMaV CRP mutant infectious clone. A CiYMaV infectious clone vector is prepared by inserting a CiYMaV complete sequence into the downstream of a 2 * 35S promoter of a vector pCASS4-RZ; the mutant is obtained by carrying out alanine site-directed mutation or deletion mutation on a CRP (C-reactive protein) region on a CiYMaV infectious cloning vector through overlap extension PCR (polymerase chain reaction), and the site-directed mutation or deletion mutation is obtained on the basis of CiYMaV strong pathogenicity infectious cloning pCiYMaV. According to the invention, a vector pCASS4-RZ is used as a vector skeleton to construct an infectious clone containing strong pathogenicity of CiYMaV, and a series of CRP mutant infectious clone which is coded by viruses and has different pathogenicity and pathogenic expressions are constructed at the same time; virus inoculation experiments show that the CRP protein affects virus accumulation and symptom formation, and a foundation is laid for research on pathogenic mechanisms, virus gene functions and low virulent line cross protection of citrus yellow mottle related viruses and subsequent Mandarivirus virus research.

An uropathogenic escherichia coli recombinant protein composition LS, a construction, expression and purification method therefor, and a use thereof, relating to the technical field of genetic engineering. The method comprises: carrying out PCR amplification on LpcA and SurA genes, and respectively inserting the LpcA and SurA genes into multiple cloning sites of a cloning vector to construct a recombinant vector; then converting the recombinant vector into an escherichia coli expression protein; and finally, carrying out purification to obtain a composition LS of an LpcA protein and an SurA protein, wherein the composition LS can be used in a urinary tract infection vaccine. The present invention has the following advantages: a protein vaccine can induce relatively strong cell and humoral immune response of animals; and animal challenge experiments show that the protein vaccine has the protection effect of reducing the bacterial loads of the bladder and the kidney.

The present invention discloses an application of an insect virus FHV RNA1 replicon in exogenous gene amplification, wherein the application amplifies the exogenous gene at the mRNA level by utilizing the autonomous replication ability of the insect virus FHV RNA1 replicon; the present invention discloses a plasmid system based on the insect virus FHV RNA1 replicon and a method for constructing the plasmid system; the present invention also discloses a method and application of the plasmid system for expressing proteins in cells, wherein the plasmid system is used to obtain a cloning vector after double enzyme digestion, and after homologous recombination with an exogenous gene, the animal or plant protein is expressed in the cell.

The present invention discloses an alfalfa pectin acetylesterase MsPAE12, its encoding gene, and its application. The amino acid sequence of the pectin acetylesterase MsPAE12 is shown in SEQ ID NO: 2, and the encoding gene sequence is shown in SEQ ID NO: 1. In addition, the present invention also provides the application of the pectin acetylesterase MsPAE12 protein and its encoding gene in increasing the number of alfalfa branches, as well as the application of its nucleotide sequence, amino acid sequence, cloning vector, expression vector, or host cell in genetic engineering. The present invention utilizes genetic engineering technology to cultivate MsPAE12 transgenic plants, significantly increasing the number of plants' branches, providing an important theoretical basis for the cultivation and breeding of new alfalfa varieties, and having great application value.

The invention belongs to the technical field of biology, and relates to a zero-background carrier as well as a preparation method and application thereof. The zero-background vector provided by the invention comprises an optimized escherichia coli CcdB gene and a corresponding escherichia coli promoter, and the nucleotide sequence of the zero-background vector is shown as SEQ ID NO.1. The zero background vector is applicable to any plasmid vector; both a cloning vector plasmid and an expression vector plasmid have relatively high connection efficiency and positive rate, and a connection product can be directly converted into a common escherichia coli strain, so that a target gene cloning vector can be effectively prepared; the target plasmid inserted with the zero background carrier has high short fragment insertion efficiency, and can also be used for long fragment insertion at the same time. In the genome assembly process, no matter whether a small fragment or a large gene fragment is inserted, the method can ensure an efficient assembly effect, especially in small fragment gene cloning, the efficiency and the positive rate can be remarkably improved, and meanwhile generation of false positive cloning is reduced.

The present invention provides gene therapies for the treatment of Friedreich's ataxia. Specifically, the present invention provides a nucleic acid, cloning vector and transfer vector for the production of an adeno-associated virus (AAV) vector. The nucleic acid comprises (i) a nucleic acid sequence encoding frataxin, (ii) a phospho-glycerate-kinase (PGK) promoter, and (iii) a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE). The present invention also provides a pharmaceutical composition which comprises the AAV vector or nucleic acid. Also, the AAV vector, nucleic acid or pharmaceutical composition can be used as a medicament, specifically as a medicament for the treatment of Friedreich's ataxia.

The invention relates to the technical field of gene engineering, in particular to a gene vector based on CsCMV mediation and a preparation method and application thereof. On the basis of a pCsCMV-CM vector, a Bsm BI site is successfully inserted into a non-coding region (UTR) behind a termination codon (TAG) at a CsCMV CP 3'end through an In-Fusion seamless cloning method, an infectious clone pCsCMV-Bs vector based on a Golden gate Coning strategy is constructed, a target gene segment can be rapidly cloned into a virus genome with high throughput, and the target gene segment can be cloned into the virus genome. The method is applied to cassava gene silencing and gene editing, the stability is higher, the phenotype duration time is longer, and a new genetic tool is provided for rapid and efficient gene function verification and directional character improvement of cassava.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for transferring an exogenous nucleic acid fragment into a plant by using a plant virus. The technical problem to be solved by the invention is to provide a new method which can be applied to transferring an exogenous fragment into a plant. According to the technical scheme, the method for transferring the exogenous nucleic acid fragment into the plant by using the plant virus comprises the following steps: fusing the exogenous nucleic acid fragment into a 3'non-coding RNA region of TVMVwt to obtain a fused fragment, constructing the fused fragment onto an infectious clone skeleton vector, and transforming the plant. According to the invention, an exogenous coding sequence is inserted into a 3'non-coding RNA region of TVMVwt to construct an infectious cloning vector, the infectious cloning vector is transferred into a plant, and expression of the exogenous coding sequence is realized.

The invention discloses an mRNA (messenger Ribonucleic Acid) quality control product of human papilloma virus as well as a preparation method and application thereof, and belongs to the technical field of in-vitro diagnosis quality control. The method comprises the following steps: chemically synthesizing an HPV E6 / E7 gene DNA sequence, respectively cloning the HPV E6 / E7 gene DNA sequence into a cloning vector to construct a synthetic plasmid template, further preparing a nucleic acid fragment containing E6 / E7 mRNA through in-vitro transcription, carrying out absolute quantification through a digital PCR technology, mixing the nucleic acid fragment with human cervical cancer cell line RNA without any HPV sequence, and screening to obtain the HPV E6 / E7 gene DNA sequence. And the mRNA quality control product with stable structure and accurate value is prepared through a vacuum freeze drying technology. The quality control product can simulate HPV E6 / E7 mRNA in a clinical sample, has comprehensive types, is suitable for accurate evaluation of sensitivity, specificity, precision and other analysis performances of an HPV E6 / E7 mRNA detection kit, provides a key quality control tool for accurate risk stratification of cervical cancer, and is suitable for various detection platforms.

The invention discloses CYP450 hydroxylase for catalyzing biosynthesis of DNJ and application of the CYP450 hydroxylase, and belongs to the field of medicinal plant genetic engineering. An enzyme catalysis experiment shows that the compound participates in a hydroxylation process of a DNJ alkaloid biosynthetic pathway, namely, C4-site stereoselective hydroxylation of (R)-2-methylpiperidine is catalyzed to generate (2R, 4R)-2-methylpiperidine-4-alcohol. The invention also relates to a gene for coding the enzyme, a cloning vector and an expression vector containing the gene, and a recombinant engineering bacterium containing the expression vector, which can be used for producing (2R, 4R)-2-methylpiperidine-4-alcohol. The method promotes the analysis of the biosynthetic pathway process of DNJ in mulberry leaves, and has important economic value and application prospect for realizing heterologous synthesis of DNJ and key intermediates.

The application provides a standard plasmid for detecting the copy number of exogenous genes of a recombinant CVA10 vaccine, and a preparation method and application thereof. The standard plasmid of the application comprises an endogenous gene MOX fragment SEQ ID No. 1, an exogenous gene P1 fragment SEQ ID No. 2 and an exogenous gene 3CD fragment SEQ ID No. 3 which are connected in series in a cloning vector. The application constructs the standard plasmid, adopts Taqman probe-fluorescence quantitative PCR method, and simply, quickly, absolutely quantitatively and accurately analyzes the copy number of inserted exogenous genes, so that the detection time of the determination of the copy number of exogenous genes of the recombinant CVA10 vaccine is effectively shortened, repeated operation is facilitated, and the determination of the copy number of exogenous genes of the recombinant CVA10 vaccine based on the Hansenula platform and the verification of the genetic stability of the exogenous genes of the recombinant CVA10 vaccine have a wide application prospect.

The present invention discloses a method for constructing a transgenic mouse with systemic overexpression of human α-Syn-NLS. First, the human α-Syn coding sequence and the SV40 nuclear translocation signal sequence are obtained by PCR. Then, an entry clone vector containing the target sequence is constructed through a BP reaction. After digestion with enzymes, the entry clone vector is constructed into a recombinant expression vector containing the target sequence through an LR reaction. After digestion with enzymes again, using the lentiviral LV overexpression vector pLV-EGFP:T2A:Puro-EF1A as the backbone vector, the recombinant expression vector pDown-hSNCA / SV40NLS containing the target sequence is constructed into the lentiviral vector, and virus packaging and purification are carried out to obtain the pLV virus overexpressing nuclear import α-Syn. The virus is injected by the method of pronuclear injection of fertilized eggs to obtain a transgenic mouse with systemic overexpression of nuclear import human α-Syn protein, which can stably overexpress α-Syn for a long time and solve the problem of nuclear import at the same time. This model has important value for studying the comprehensive functions and mechanisms of α-Syn nuclear import.

The invention belongs to the technical field of molecular biology, and particularly relates to a sesame flowering regulation gene SiHd3a; the gene is located in a section of 13.54 Mb to 13.95 Mb on a No.11 chromosome of sesame, and is a heading data 3a of the sesame, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1; the amino acid sequence of the encoded protein is as shown in SEQ ID NO. 2; the invention also discloses application of the plant growth regulator in regulating the flowering time of plants. Connecting to form a cloning vector containing the SiHd3a gene by using a homologous recombination technology, and constructing an expression vector for plant expression; the invention further discloses a cloning vector containing the SiHd3a gene and application of an expression vector constructed by the cloning vector in regulating the flowering time of plants. The sequence of the sesame flowering regulation gene SiHd3a is disclosed for the first time, the problems of flowering asynchronism and introduction in plant early flowering breeding and cross breeding are solved by utilizing overexpression of the SiHd3a gene, plant flowering can be remarkably promoted, the growth period can be remarkably shortened, and gene resources are provided for plant early flowering breeding and introduction.

The application of Trifolium repens TrWRKY7c1 gene in improving plant cold resistance belongs to the technical field of plant genetic engineering and crop genetic breeding. In order to solve the technical blank of the existing technology about the research on low-temperature stress related genes of Trifolium repens, the application of Trifolium repens TrWRKY7c1 gene in improving plant cold resistance is provided, the nucleotide sequence of the gene is shown as SEO ID NO. 1, and the amino acid sequence encoded by the gene is shown as SEO ID NO. 2; a recombinant cloning vector, a plant expression vector or a recombinant glycerol preservation strain containing the gene are also provided, and a transgenic plant is obtained through an agrobacterium-mediated genetic transformation method. The TrWRKY7c1 gene provided in the application can be applied to improve plant cold resistance, cultivate cold-resistant plants, and provide a new gene resource for plant cold resistance breeding, and has important significance for the improvement of cold resistance and the research on the regulation mechanism of plant growth and development.

The invention discloses a construction method and application of a chicken anemia virus full-length gene infectious clone plasmid carrying an EGFP tag, and belongs to the technical field of virus gene engineering. According to the invention, a cyclic genome of CAV is linearized by utilizing a reverse genetic technology, and the linearized CAV genome is connected with a cloning vector pcDNA3.1 (+) by virtue of a seamless cloning method, so that a CAV full-length infectious clone pcDNA3.1-CAV and a CAV full-length infectious clone pcDNA3.1-CAV-EGFP (Enhanced Green Fluorescent Protein) carrying an EGFP (Enhanced Green Fluorescent Protein) tag are successfully constructed; in addition, in-vitro virus rescue is realized on MDCC-CU147 cells, and pathogenicity consistent with that of CAV is realized in SPF chicken bodies. The invention provides a simple and rapid CAV reverse genetics operating system, and the CAV carrying the EGFP green fluorescent protein gene is successfully obtained. The method has wide application value in the aspects of research on a CAV pathogenic mechanism and a virus replication mechanism, research and development of CAV vaccines and diagnostic reagents and the like.

It provides an inhibitor of complement activation. [Solution] An isolated monoclonal antibody and its antigen-binding fragment are provided that specifically bind to an epitope within the serine protease domain of human MASP-2. In some embodiments, the antibody or its antigen-binding fragment inhibits lectin pathway complement activation. Polynucleotides encoding the monoclonal antibody or its antigen-binding fragment, and cloning vectors or expression cassettes containing such polynucleotides are also provided. Methods for inhibiting lectin pathway complement activation and methods for treating diseases and disorders of the lectin pathway are further provided.

The disclosure provides for methods of constructing synthetic chromosomes including the steps of providing host cells with an endogenous chromosome, transforming a cloning vector and a cloning cassette into the host cells, excising target genomic nucleic acids from the endogenous chromosome, recombining the excised target genomic nucleic acids with the cloning cassette via homologous recombination to form heterologous vectors comprising cloned sequences, extracting the heterologous vectors containing the cloned sequences from the host cells, digesting the heterologous vectors with a restriction endonuclease to release the cloned sequences from the heterologous vectors to provide released cloned sequences, and introducing the released cloned sequences, a centromere cassette, and a yeast artificial chromosomes or bacterial artificial chromosomes into a second host cell, such that the released cloned sequences, the centromere cassette, and the YAC or BAC recombine with one another via homologous recombination to produce the synthetic chromosome.

The present invention relates to the field of gene engineering technology, and specifically to a gene vector mediated by CsCMV, and a preparation method and application thereof. Based on the pCsCMV-CM vector, the present invention successfully inserts the Bsm BI site into the non-coding region (UTR) after the stop codon (TAG) at the 3' end of CsCMV CP by the In-Fusion seamless cloning method, and constructs an infectious cloning pCsCMV-Bs vector based on the Golden gate Cloning strategy, which can quickly and high-throughput clone the target gene fragment into the viral genome, and is applied to cassava gene silencing and gene editing with higher stability and longer phenotypic duration, providing a new genetic tool for rapid and efficient gene function verification and directional improvement of cassava traits.

The invention provides a tomato late blight resistant gene SlPin4. The nucleotide sequence of the tomato late blight resistant gene SlPin4 is as shown in SEQ ID NO. 1; the invention provides a cloning method and application of the SlPin4 gene. The cloning method of the SlPin4 gene comprises the following steps: amplifying by using a PCR (Polymerase Chain Reaction) technology by taking a genome of a wild tomato early pollen No.2 as a template; the method comprises the following steps: connecting an amplified and purified PCR (Polymerase Chain Reaction) product with a pMD-19T cloning vector, transforming the constructed pMD-19T-SlPin4 into escherichia coli DH5alpha, selecting a single colony, shaking bacteria, extracting plasmids, sequencing, and indicating that a recombinant expression vector pBI121-SlPin4 is successfully constructed if the sequencing is correct; the invention also provides an application method of the gene, and overexpression of the SlPin4 can positively regulate and control disease-resistant related genes in tomatoes, so that the disease resistance of tomato plants is improved. The resistance of tomatoes to late blight is enhanced through transient overexpression of the SlPin4 gene, and the SlPin4 gene is of great significance to cultivation of disease-resistant tomatoes and increase of both production and income.

The invention discloses a dermatophagoides pteronyssinus allergen Der p 1 recombinant protein, a preparation method, a kit and application, relates to the technical field of biology, and solves the technical problem that a detection result is not accurate enough when a crude extract is used for allergen detection. The method comprises the following steps: designing a pair of specific primers for amplifying Der p 1, carrying out amplification through PCR (Polymerase Chain Reaction), recovering a target band, connecting the target band with a cloning vector, converting to competent cell culture, extracting to obtain a recombinant plasmid containing a target gene, cloning the recombinant plasmid to a prokaryotic expression vector to obtain an expression vector containing the target gene, and carrying out high-throughput screening on the expression vector containing the target gene. The method comprises the following steps: transforming an expression vector containing a target gene into a host cell, inducing expression, and purifying to obtain Der p 1 recombinant protein with an amino acid sequence as shown in SEQ ID NO.1; the recombinant protein is high in biological activity, can be applied to preparation of a magnetic particle chemiluminescence detection kit, and is high in detection accuracy and good in kit stability.

The present invention relates to a process for constructing a single vector comprising cas9 and sgRNA (“all-in-one” vector) for mycobacterial genome modifications, comprising the steps of (a) providing the expression cassette of cas9 and the tracrRNA expression cassette with codons optimized for expression in mycobacteria and clone into a cloning vector; (b) sequentially cloning the sequences optimized in step (a) into a mycobacterial bridge vector; (c) selecting targeting sequences (crRNAs) specific to the gene of interest; (d) inserting the crRNAs selected in step (c) into the mycobacterial expression vector, upstream of the tracrRNA sequence, at position +1 downstream of an inducible promoter for the construction of the sgRNA cassette; and (e) adding at the end of each expression cassette a transcriptional terminator.

The invention discloses a preparation method and application of ackermania mucophilia outer membrane protein transgenic tomatoes. The preparation method provided by the invention comprises the following steps: transforming a codon-optimized ackermann mucophile outer membrane protein gene into competent cells through a cloning vector, and infecting a cultured tomato explant by adopting the transformed competent cells to obtain the ackermann mucophile outer membrane protein. And culturing the transgenic tomato expressing the ackermann mucophile outer membrane protein by adopting the infected explant. According to the preparation method, the AMUC1100 protein can be expressed in tomatoes, the tomato fruits are used for preventing or treating the metabolic dysfunction related fatty liver diseases, the treatment effect of the AMUC1100 protein can be obtained, the functions of the tomatoes can be coordinated, and the effect of preventing or treating the metabolic dysfunction related fatty liver diseases is achieved. A new scheme and strategy are provided for prevention or treatment of metabolic dysfunction related fatty liver diseases; in addition, the problems of infection risk in viable bacteria treatment, high viable bacteria storage and transportation cost and the like are solved.

A uropathogenic Escherichia coli recombinant protein composition LS, its construction, expression, purification method and application, belong to the field of genetic engineering technology. The present invention performs PCR amplification on LpcA and SurA genes and inserts them into the multiple cloning sites of cloning vectors respectively to construct a recombinant vector; then the recombinant vector is transformed into Escherichia coli to express the protein; finally, purification is performed to obtain a composition LS of LpcA protein and SurA protein, and the composition LS can be used in urinary tract infection vaccines. The advantages of the present invention are that the protein vaccine can induce stronger cellular and humoral immune responses in animals; animal challenge experiments show that it has a protective effect of reducing the bacterial load of bladder and kidney.

The invention discloses an early warning factor detection method for SAA-ichthyophthirius multifiliis infection, and particularly relates to the technical field of SAA-ichthyophthirius multifiliis infection detection.The specific steps are as follows: S1, RNA extraction; s2, removing reverse transcription of the genome DNA and cDNA (complementary deoxyribonucleic acid); s3, constructing a cloning vector of the Saa CDS region; s4, constructing an Saa phylogenetic tree; s5, constructing a Saa fluorescent quantitative detection primer design and standard curve; s6, performing saa expression after the ichthyophthirius multifilis infects the pelteobagrus vachelli; and S7, data statistics and analysis. Pelteobagrus vachelli is taken as a research object, a saa CDS region of the pelteobagrus vachelli is cloned and analyzed, a saa fluorescent quantitative detection method is established, saa mRNA expression level is dynamically monitored at different stages of ichthyophthirius multifiliis infected by the pelteobagrus vachelli, the reaction time and part of the saa in the ichthyophthirius multifiliis infection are disclosed, and the application of the saa fluorescent quantitative detection method to the detection of the ichthyophthirius multifiliis infection is realized. A foundation is laid for function analysis and application research of saa, and a foundation is laid for early prevention and early control of ichthyophthiriasis.

The invention discloses an infectious pseudo-ginseng virus A cloning vector, the nucleotide sequence of the infectious pseudo-ginseng virus A cloning vector is shown as SEQ ID NO: 1, the infectious pseudo-ginseng virus A cloning vector belongs to the field of plant virology and genetic engineering, and the infectious pseudo-ginseng virus A cloning vector is obtained by cloning CP gene and RdRp gene of pseudo-ginseng virus A into a pCB301 vector; on the basis of the vector, a PnVA infectious cloning vector pPnVA-GFP carrying a green fluorescent protein GFP is constructed; a PnVA infectious cloning vector is transferred into an agrobacterium GV3101 strain through a freeze-thaw method, the agrobacterium GV3101 strain is transferred into nicotiana benthamiana and pseudo-ginseng through an injection method for expression, and results show that pCB301-PnVA and pPnVA-GFP can complete PnVA gene replication in pseudo-ginseng and tobacco and trigger typical virus disease symptoms.

The invention belongs to the technical field of synthetic biology, and particularly relates to a system and a method for cloning toxic genes, and the system comprises a host transformed by a cryptographic subsystem, a cloning vector transformed by a codon and a target gene transformed by the codon. By using the system, the expression of the toxic gene can be completely blocked, and the problem that the toxic gene protein expression product seriously interferes with the growth state and mutation of cells of hosts such as escherichia coli is solved, so that the target gene can be successfully cloned in an anti-toxic strain, and the success rate and timeliness of gene synthesis are guaranteed.