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Soybean mosaic virus major gene locus and application thereof

A mosaic virus and leaf virus technology, applied in DNA/RNA fragmentation, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems of small effect value and difficult application, and achieve reliable use, accurate and rapid screening and detection. The method is convenient and fast effect

Inactive Publication Date: 2013-05-22
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also some reports on the QTL mapping of soybean resistance to SMV. At present, QTLs for soybean mosaic virus resistance have been mapped on soybean chromosomes 2, 13, and 14, but the effect value is small, and it is difficult to apply in soybean breeding.

Method used

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  • Soybean mosaic virus major gene locus and application thereof
  • Soybean mosaic virus major gene locus and application thereof
  • Soybean mosaic virus major gene locus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Using the primer pair GmSSR13-23 to identify the F of Zhongdou 29×Zhongdou 32 11 Mosaic Virus Resistance Traits in Families

[0042] 1. PCR reagents for identification or auxiliary identification of soybean mosaic virus resistance traits

[0043] The PCR reagent for the identification or auxiliary identification of soybean mosaic virus resistance traits in this embodiment consists of PCR primer pair GmSSR13-23, 10×Taq buffer, dNTP mixture, MgCl 2 solution, Taq DNA polymerase and ddH 2 O composition.

[0044] Among them, the PCR primer pair GmSSR13-23 consists of two single-stranded DNAs, a forward primer and a reverse primer, and its sequence is as follows:

[0045] Forward primer: 5'-CAGCCAATAGCAACAAAGGG-3' (SEQ ID No.1),

[0046] Reverse primer: 5'-GCCTTGCTTGCTCATCAAAT-3' (SEQ ID No.2).

[0047] 2. Soybeans to be identified

[0048] Middle Bean 29 × Middle Bean 32 F 11 The family was obtained as follows: Zhongdou 29 and Zhongdou 32 were crossed, and ...

Embodiment 2

[0122] Example 2, the acquisition of the soybean anti-mosaic virus main effect gene locus and primer pair GmSSR13-23 of the present invention

[0123] The segregation population used is the soybean cultivar Zhongdou 29 (with a disease index of 65) and Zhongdou 32 (with a disease index of 12) that have significantly different resistance to mosaic virus SC-3 strains, and 406 soybean families were derived Recombinant inbred line population (Zhou Xin'an et al., Three- and four-pod variation of soybean recombinant inbred line population and its relationship with yield, Chinese Journal of Oil Crops, 2005, 27: 22-25). 165 families were randomly selected from the recombinant inbred line population (Zhou Rong et al., QTL analysis of soybean seedling root traits, Acta Crops Sinica, 2011, 37:1151-1158), and the method of artificial friction inoculation was used to identify 165 families for SMV SC Resistance of -3 strains.

[0124] The construction method of the isolated population is th...

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Abstract

The invention discloses a soybean mosaic virus major gene locus and application thereof, and in particular relates to the soybean mosaic virus major gene locus and the application of the soybean mosaic virus major gene locus in molecule marker assistant selective breeding. The soybean mosaic virus major gene locus is qRSC.13-1 with a contribution rate of 32.4%; the molecule which is tightly interlocked with the mosaic virus major gene locus is marked as GmSSR13-23. The tightly interlocked molecule marker is utilized to detect whether the breeding population system contains the major gene locus or not, so that the mosaic virus performance can be predicated, and the selection rate of the soybean mosaic virus breeding is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology and genetic breeding. More specifically, it relates to a soybean anti-mosaic virus main effect gene site and a molecular marker closely linked to the main effect gene site, and also relates to the application of the molecular marker in soybean mosaic virus resistance breeding. Background technique [0002] Soybean is an important crop, rich in protein and fat and active ingredients beneficial to the human body such as soap II, isoflavones, phospholipids, etc. It is one of the main sources of vegetable protein and oil in the world. At present, my country's soybean planting area, total output and per unit yield rank fourth in the world, and its production level lags behind the world's soybean production. In 2012, China's soybean planting area was 7.18 million hectares, with a total output of 12.8 million tons, while imported soybeans reached a record 57.5 million tons, accounting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 陈海峰沙爱华单志慧杨中路张晓娟张婵娟陈李淼陈水莲邱德珍周蓉吴学军周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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