259results about How to "The detection method is convenient and fast" patented technology

The invention discloses a wireless charging, alignment and metal foreign body detection system of an electric automobile and a method thereof. The detection system comprises a transmitting coil, a receiving coil, a probe, a signal conditioning module and a control module connected with the signal conditioning module, wherein the transmitting coil is connected with the output end of a wireless charging and transmitting device on the ground surface of a parking lot, the receiving coil is connected with the input end of a wireless electric energy receiving device of an electric automobile chassis and is mutually coupled with the transmitting coil, and the probe is laid on the surface of the transmitting coil and is mutually coupled with the transmitting coil and the receiving coil. The probe is of an axis-symmetric structure and is composed of two coils equal in size and turn number and opposite in winding direction. The probe is consistent with the boundary of the transmitting coil in size. The signal conditioning module is used for receiving voltage signals output by two output ends of the probe and conducting amplification, filtration and peak-holding processing on the two signals output by the two output ends. The control module is used for obtaining the difference of the two received voltage signals and comparing the obtained voltage signal difference value with a preset voltage threshold value.

The invention discloses a molecular marker in close linkage with rape crotch angle character QTL (Quantitative Trait Loci) and application. The molecular marker is characterized by carrying out hybridization by taking cabbage type rape variety Shanghai oil 19 as a female parent and Purler as a male parent, establishing F2 segregation population through selfing of hybrid F1, and analyzing, thus obtaining a crotch angle locus qBA1.A06; designing a primer by utilizing an Indel marker at the boundary of the crotch angle locus qBA1.A06 so as to detect the parents and the F2 population, thus obtaining a molecular marker BAIndel76 and a molecular marker BAIdenl79 which are in close linkage with the crotch angle character QTL; identifying a rape gene type formed after hybridization of the two parents by utilizing a marker primer, and carrying out auxiliary selection by utilizing the marker, thus greatly increasing the selection efficiency. According to the molecular marker disclosed by the invention, a novel genetic marker is provided for molecular breeding of the rape plant type, and useful information is also provided for accurate positioning on the crotch angle character QTL of the cabbage type rape and map-based cloning on related genes.

The invention discloses a rapeseed pod number major QTL molecular marker and its application. The marker can be used for marker-assisted selection in pod number character improvement and fine mapping and map-based cloning of QTL site. Primers of the molecular marker are CNU400F:5'-CGAGTTTTTGTGTGTACGTATAGTAAT-3' and CNU400R:5'-CCAAAGTGCGTAAAGGAAGG-3'. Rapeseed Zhongshuang 11 and 73290F3 generation are selected by the use of the marker, and silique number of the screened F3 single plant is higher than that of Zhongshuang 11 by 92.3%. Thus, the marker is utilized for assistant selection so as to greatly enhance selection efficiency of high yield breeding.

The invention discloses a method for automatically detecting dead pixels of a mobile phone screen before assembling. According to the method, detection is carried out before components of the mobile phone screen are assembled, the components of the mobile phone screen are fixed temporarily through an installation seat and provided with voltages of different voltage values so as to display pure-color pictures of different colors, a high-definition camera is used for taking pictures, image analysis software of a computer is used for analyzing the pictures, and whether the components of the mobile phone screen have the dead pixels can be quickly detected. The detection method is simple, convenient and quick to implement, and can detect whether the mobile phone screen has the dead pixels before assembling, parts of subsequent detection items are reduced, the phenomena that mobile phones are returned to a production line to be repaired or the mobile phone screens are replaced or mobile phones are scrapped are subsequently reduced, production efficiency is improved, and production cost is lowered.

The invention discloses an asphalt pavement structure depth calculation method based on a generalized regression neural network. The method comprises steps of collecting two-dimensional images of thesurfaces of the prepared concrete test piece before and after sand laying; reconstructing a pavement texture three-dimensional model according to the gray value of the two-dimensional image; calculating a sand paving plane of the sand paving image by using a digital image processing technology; meanwhile, the sand laying plane is also a reference surface of the pavement texture three-dimensional model; fitting the pavement texture three-dimensional model above the reference surface by adopting a least square method, and generating a fitting curved surface; calculating the volume between the two sides of the reference surface and the three-dimensional model according to an integration method; wherein the ratio of the volume to the projection area of the pavement texture three-dimensional model on the horizontal plane is the average elevation Ha of the asphalt concrete test piece image; and taking the average elevation Ha, the extreme value and the gray average value of the test piece image as input samples of a generalized regression neural network, taking the actual pavement construction depth as output samples, carrying out neural network model training, and predicting the pavement construction depth by using the trained model, with the prediction precision reaching 90% or more.

The invention provides a key SNP molecular marker related to content of linolenic acid in camellia oleifera seed kernel oil and application of the key SNP molecular marker. The molecular marker is anyone selected from PB. 70158. 1-473, PB. 70158. 1-649, PB. 70158. 1-704 and PB. 70158. 1-707. The SNP marker provided by the invention is definite in position, convenient and rapid in detection method, free of environmental influence, higher in sense of purpose, low in workload, higher in efficiency and low in cost. Through detecting these SNP loci, identification and auxiliary screening can be carried out at a seedling stage, the production cost is reduced greatly, and the efficiency of selection is increased greatly. Through breeding camellia oleifera with high linolenic acid content by using the molecular marker provided by the invention, the efficiency of selection of breeding of the camellia oleifera can be increased, and a breeding progress is accelerated.

The invention relates to the technical field of molecular markers, and specifically relates to SNP molecular markers related to stearic acid content in camellia oleifera seed oil and applications of the SNP molecular markers. Two SNP molecular markers PB.35271.1-548 and PB.35271.1-555 related to the stearic acid content in camellia oleifera seed oil are provided; the SNP molecular marker PB.35271.1-548 contains the nucleotide sequence shown as SEQ ID NO.1 and of which polymorphism is C/T at the position 548th site; and the SNP molecular marker PB.35271.1-555 contains the nucleotide sequence shown as the SEQ ID NO.1 and of which polymorphism is G/A at the position 555th site. Through the utilization of the two markers to identify the stearic acid content phenotype of camellia oleifera, theidentification and auxiliary screening of seedling stages can be realized, the selection efficiency of camellia oleifera breeding can be effectively enhanced, and breeding process can be accelerated.

The invention discloses a through-silicon via (TSV) testing structure and a TSV testing method. The TSV testing structure comprises a semiconductor substrate, an interlayer dielectric layer located on the surface of the semiconductor substrate, a TSV formed in the semiconductor substrate and the interlayer dielectric layer, and at least one interconnection structural ring, wherein the interconnection structural ring is distributed in an annular shape on the periphery of the TSV regarding the TSV as the center and is formed by metal layers on different layers, and electric conduction plugs between the metal layers in a serial-connection mode. Due to the fact that the interconnection structural ring is distributed in the annular shape on the periphery of the TSV regarding the TSV as the center, resistance of the interconnection structural ring is tested in directions far away from the TSV in sequence, tested resistance values are compared with a reference resistance value, then the range of an isolation region between the interconnection structural ring and the TSV can be obtained, and therefore the TSV testing structure and the TSV testing method are convenient and fast to use.

The invention discloses a method and a model for predicting the lodging resistance of soybeans. The method provided by the invention comprises the steps of (1) respectively measuring the numerical values of the following seven characters of the soybeans to be measured: stalk strength, plant height, stem diameter, branching number, root length, root weight and overground weight; (2) substituting the numerical values of the stalk strength, the plant height, the stem diameter, the branching number, the root length, the root weight and the overground weight into the lodging resistance model of the soybeans to calculate the lodging resistance: LR=174.162-6.618*BN-2.643*PH+15.165*RW+5.549*SD+0.108*SS+0.027*RL-0.483*SW, wherein if LR is more than or equal to 75, the soybeans to be measured are lodging-resistant soybeans or candidate lodging-resistant soybeans; if LR is less than 75, the soybeans to be measured are easy-lodging soybeans or candidate easy-lodging soybeans. The experiments prove that the method is convenient and fast, and is not influenced by environment.

The invention relates to the technical field of molecular markers, in particular to an SNP molecular marker related to palmitoleic acid content in camellia oleifera seed grease and application thereof. The invention provides two SNP molecular markers PB.10070.2-202 and PB.7527.1-77 related to palmitoleic acid content in camellia oleifera seed grease. The PB.10070.2-202 contains a nucleotide sequence with polymorphism of G/A at the 202nd site of the sequence shown as SEQ ID NO.1; the PB.7527.1-77 contains a nucleotide sequence with polymorphism of G/A at the 77th site of the sequence shown as SEQ ID NO.2. The two markers are used for identifying the palmitoleic acid content phenotype of camellia oleifera, seedling stage identification and auxiliary screening can be achieved, the selection efficiency of camellia oleifera breeding is effectively improved, and the breeding process is accelerated.

The invention discloses a molecular marker of a pot shattering resistance trait major gene locus of rapes and an application. The steps are as follows: 1) hybridizing double 11 variety and 73290 variety (which have obvious differences in the pot shattering resistance traits) in a cabbage type rape varieties, and carrying out selfing on progenies to obtain F2 and F2:3 segregation populations with the segregated pod shattering resistance traits; 2) carrying out polymorphic screening on parent DNA (deoxyribonucleic acid) by utilizing an SSR (simple sequence repeat) primer, and establishing a genetic linkage map by carrying out SSR molecular marker genotyping on the F2 progeny segregation populations; 3) carrying out field experiments and plant inquisition on the F2 and F2:3 segregation populations so as to obtain phenotype data of the pod shattering resistance traits; and 4) carrying out QTL (quantitative trait locus) detection by combining with the developed high-density molecular marker genetic linkage map and genotype and the phenotype data of the segregation populations, utilizing QTL Cart2.5 software to obtain the major gene locus Psr.A9 for controlling the pod shattering resistance of the rapes on a cabbage type rape A9 linkage group and also obtain the molecular marker BrSF0007-39 closely linked with the major gene locus. The selection efficiency of breeding with the pod shattering resistance can be greatly improved by utilizing the marker for assistant selection.

The invention discloses a streptomycin sulfate detection method. The method is implemented through a specific reaction of a chemical fluorescent probe with streptomycin sulfate and comprises steps as follows: 1) dissolving the chemical fluorescent probe in an organic solvent to obtain a solution A, and dissolving alkali in water to obtain a solution B; 2) dissolving the streptomycin sulfate in water to prepare a series of streptomycin sulfate aqueous solutions with different concentrations, that is, standard solutions; 3) mixing and oscillating the solution A and the solution B with the standard solutions with the different concentrations respectively, leaving the mixtures to stand for layering, transferring water layers, measuring the fluorescence intensity, and establishing a correlation curve about the fluorescence intensity and the concentrations of the standard solutions; 4) mixing and oscillating the solution A and the solution B with a to-be-detected sample solution, leaving the mixture to stand for layering, transferring a water layer, measuring the fluorescence intensity, comparing the curve with the curve established in Step 3), and calculating the content of the streptomycin sulfate in a sample.

The invention provides a rapid detection method of sulfathiazole in an animal body, and a Raman characteristic peak of sulfathiazole is determined by using a density functional theory. The method comprises a step of establishing a quantitative analysis curve according to the characteristic peak intensity with high Raman peak intensity and good peak pattern. Standard recovery experiments show thatthe method is good in accuracy and precision. According to the method, the lowest detection concentration of sulfathiazole in an animal body is 1 mg/L. According to the method, the sulfonamide antibiotic residues in the animal body are qualitatively and quantitatively analyzed, single sample detection is completed within 3 min, and a rapid and convenient detection method is provided for rapid qualitative screening and preliminary quantitative detection of the sulfonamide thiazole residues in the animal body.

The invention relates to a method for visually detecting nucleic acid, which is a method by adopting nucleic acid as target and connecting magnetic beads and gold nano-particles by chemical self-connection reaction. According to the method, the reaction condition is controlled, a connecting probe complementary with the target nucleic acid part is respectively modified on surfaces of magnetic beadsand gold nano-particles, and the magnetic beads and gold nano-particles are connected by a self-connection reaction after matching with the target nucleic acid; the gold nano-particles which do not react are removed through magnetic separation, and visual detection on nucleic acid can be carried out through magnetic separation. The separation method is convenient, and materials are partially commercialized with high biosafety.

The invention provides close linkage simple sequence repeat (SSR) molecular markers of a main effect quantitative trait locus (QTL), namely qRBSDV-6 for resistance to rice black streaked dwarf viral disease, and a method for improving the resistance of susceptible rice varieties through marker assistant selection. For derived line segregating population obtained by hybridizing and back-crossing Minghui 63 serving as a resistance source and a susceptible receptor parent, genotypes of the close linkage markers RM7158 and RM587 on two sides of the main effect quantitative trait locus (QTL), namely qRBSDV-6 for resistance to black streaked dwarf viral disease on the short arm of the sixth chromosome of Minghui 63 are detected, the close linkage markers can be successfully imported into the genetic background of the susceptible rice varieties, the resistance of the susceptible rice varieties to the black streaked dwarf viral disease is effectively improved and the selection efficiency reaches 92.31 percent. The method is easy to implement, quick and efficient, and is an effective method for improving the resistance to the rice black streaked dwarf viral disease.

The invention discloses primers for identifying an avian infectious bronchitis virus strain type, an RT-PCR detection kit, a method and application. Sequences of the primers are as follows: an H120 forward primer: 5'-ATTGCTTACGGTCCTCT-3', and an H120 reverse primer: 5'-CTGCTGGTTGACATCTT-3'; a QX forward primer: 5'-GTACAGGGTCTTGTCCTA-3', and a QX reverse primer: 5'-GTGTTGCTTAATTCACCT-3'; an LDT3 forward primer: 5'-CGCCACAGCAGGAAGAAT-3', and an LDT3 reverse primer: 5'-GTCCGTAGTTGGAATGAAGA-3'; a 4/91 forward primer: 5'-CCAGATAGGCGGTGTTAG-3', and a 4/91 reverse primer: 5'-TCGGCAATAGGAAAGTGT-3'. The primers, the RT-PCR detection kit, the method and the application have the beneficial effects that the kit provided by the invention separately amplifies four fragments of different sizes by only once gradient RT-PCR, and then, vaccine strains and popular wild strains of infectious bronchitis of chickens can be rapidly identified and diagnosed; the kit has the characteristics of high efficiency,low cost, high specificity, high sensitivity and the like; a convenient and quick detection method is provided for the differential diagnosis and molecular epidemiological analysis of avian infectious bronchitis viruses and prevention and control of the avian infectious bronchitis viruses.

The invention relates to the technical field of molecular markers, in particular to an SNP molecular marker related to oleic acid and linoleic acid content in camellia oleifera seed oil and application thereof. The invention provides three SNP molecular markers PB.34365.1-621, PB.8541.2-812 and PB.35271.1-555 which are related to the content of oleic acid and linoleic acid in camellia oleifera seed oil. The SNP molecular markers respectively contain nucleotide sequences with polymorphisms of C/T, T/C and G/A at the 621st, 812th and 555th sites of the sequences shown as SEQ ID NO.1-3. The threemolecular markers are used for identifying the content phenotypes of oleic acid and linoleic acid of camellia oleifera, seedling stage identification and auxiliary screening can be achieved, the selection efficiency of camellia oleifera breeding is effectively improved, and the breeding process is accelerated.

A method for measuring the VFA concentration in the anaerobic wastewater treatment process by means of near infrared spectrum comprises the steps of 1, taking N wastewater samples in the anaerobic wastewater treatment process within the typical cycle, measuring the VFA concentration of the N wastewater samples with the gas chromatographic method, and measuring the original near infrared spectrum of the N wastewater samples by means of an near infrared spectrometer; 2, pretreating original near infrared spectrum data through wavelet transform; 3, establishing a calibration model based on the VFA concentration of the N wastewater samples and a pretreated spectrogram by means of interval partial least squares, calculating the correlation coefficient, adopting the calibration model when the correlation coefficient is larger than or equal to 0.945, and repeating step 1-3 and conducting modeling again and correlation evaluation otherwise; 4, measuring the original near infrared spectrum of a wastewater sample to be measured, conducting pretreatment, and substituting pretreated spectrum data into the calibration model to obtain a VFA concentration value. The method is convenient to operate, low in cost, environmentally friendly, and capable of obtaining a result quickly and effectively.

The invention discloses a method for detecting pig viral diseases on the basis of TEM-PCR and a gene chip. The method comprises the steps that according to a target sequence, enriching multiplex-PCR, adopting four pairs of specific nested PCR primers and a probe, and adding a tag sequence which can be recognized by a pair of universal primers respectively on the 5 prime end of each inner primer. According to the method, a lot of defects of a traditional PCR method are overcome, the compatibility, specificity, flexibility and the like are all better than the traditional PCR method; the reaction conditions of Tem-PCR are optimized, and an efficient and accurate, and convenient and quick detection method is established; the background noise of the reaction is reduced, and the fund is greatly saved; an advanced Tem-PCR multiple amplification technology and a gene chip detection technology are combined for the first time, a system through which four etiologies can be detected on one piece of chip at the same time is established, and a foundation is laid for later molecular biology detection of important pathogenic microorganism.

The invention discloses a primer composition for detecting pepper mottle potyvirus as well as application of the primer composition, a kit consisting of the primer composition and application of the kit. The primer composition comprises an outer primer and an inner primer, wherein the outer primer comprises an outer primer F3 and an outer primer B3; the DNA sequence of the outer primer F3 is as shown in SEQ ID NO.1; the DNA sequence of the outer primer B3 is as shown in SEQ ID NO.2; the inner primer comprises an inner primer FIP and an inner primer BIP; the DNA sequence of the inner primer FIP is as shown in SEQ ID NO.3; the DNA sequence of the inner primer BIP is as shown in SEQ ID NO.4. The primer composition and the kit consisting of the primer composition can be applied to rapid diagnosis and identification of pepper mottle potyvirus, and have the advantages of good detection sensitivity, good specificity, simplicity, convenience and rapidness in operation, and the like.

The invention provides a rape pod shatter relevant gene, a molecular marker and application. An applicant positions a negative effect QTL site for controlling the anti-pod-shatter effect on rape A09 for the first time; 11.26 to 12.07 percent of phenotypic variance can be explained; a pair of InDel marks (IF4/IR4) are further developed by using the differences of R1 and R2 genomes; pod anti-pod-shatter different QTL sites are detected through gene function marks; and elimination can be performed in the seedling period, so that the production cost is reduced, and the selection efficiency is greatly improved. The positions of function genes of the anti-pod-shatter negative effect QTL site are specific; a detection method is convenient and fast; and the environment influence is avoided. The anti-pod-shatter performance of breeding materials can be predicted through detecting the anti-pod-shatter property function molecular marker; and an anti-pod-shatter rape strain can be further accurately and fast screened.

The invention belongs to the technical field of molecular biology and genetic breeding, and particularly discloses a molecular marker BrSF0239 primer for main-effect QTL loci in florescence and maturity of Brassica napus and an application thereof. Phenotypic data of florescence characters were obtained by field experiments and character investigation on F2 and F2: 3 pedigree segregated populations of Zhongshuang No. 11 (a kind of oilseed rape) and 73290 (a kind of oilseed rape); genotype and genetic map of the F2 segregated populations are combined for QTL detection, and the main-effect geneloci, on an A2 linkage group, for joint controlling the florescence and the maturity are obtained, specifically, the contribution rate to the florescence of oilseed rape is 17.2% with additive effect2.1 and dominant effect 0.9; and the contribution rate to the maturity of the oilseed rape is 9.4% with the additive effect 0.6 and the dominant effect 0.4. By detecting the molecular markers relatedto the characters in the maturity, the length of the maturity can be predicted, and then early-maturing varieties can be selected quickly and accurately.