Primers and method for improving resistance of susceptible rice varieties to black streaked dwarf viral disease

A black-streaked dwarf disease and rice technology, applied in the field of molecular genetics, can solve problems such as environmental pollution and difficulty in achieving expected results, and achieve the effects of clear location, convenient identification, and accelerated cultivation process

Active Publication Date: 2012-09-19
JIANGSU LIXIAHE REGION AGRI RES INST
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AI Technical Summary

Problems solved by technology

[0004] In terms of production, the current prevention and control of rice black-streaked dwarf disease mainly combines the adjustment of farming system, the improvement of cultivation technology, and the prevention and control of diseases and insect pests at the right time, but it is often difficult to achieve the expected effect and cause great pollution to the environment

Method used

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  • Primers and method for improving resistance of susceptible rice varieties to black streaked dwarf viral disease
  • Primers and method for improving resistance of susceptible rice varieties to black streaked dwarf viral disease
  • Primers and method for improving resistance of susceptible rice varieties to black streaked dwarf viral disease

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Embodiment 1

[0028] 1) Under the genetic background of Huaidao 5, the carrier " qRBSDV-6 The construction of the near isogenic line of "

[0029] F 1 , and then use "Huaidao No. 5" as the recurrent parent for continuous backcrossing, from BC 1 f 1 From the beginning of the segregation generation, for different rice single plants, use the target resistance QTL provided by the present invention" qRBSDV-6 The SSR markers RM7158 and RM587 on both sides were used for genotype detection. The DNA was extracted using the conventional CTAB method.

[0030] PCR amplification system: 0.2μmolL -1 1.5 μL each of the forward and reverse primers, 200 μmolL -1 dNTPs 2μL, 10×PCRbuffer 2μL, 50~100ng DNA template 2μL, 1UμL -1 of tag Enzyme 0.3 μL, ddH 2 O10.7 μL.

[0031] The PCR reaction conditions were: pre-denaturation at 94°C for 5 min, denaturation at 94°C for 1 min, renaturation at 55°C for 1 min, extension at 72°C for 1 min, 35 cycles, and finally extension at 72°C for 5 min.

[0032] Selec...

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Abstract

The invention provides close linkage simple sequence repeat (SSR) molecular markers of a main effect quantitative trait locus (QTL), namely qRBSDV-6 for resistance to rice black streaked dwarf viral disease, and a method for improving the resistance of susceptible rice varieties through marker assistant selection. For derived line segregating population obtained by hybridizing and back-crossing Minghui 63 serving as a resistance source and a susceptible receptor parent, genotypes of the close linkage markers RM7158 and RM587 on two sides of the main effect quantitative trait locus (QTL), namely qRBSDV-6 for resistance to black streaked dwarf viral disease on the short arm of the sixth chromosome of Minghui 63 are detected, the close linkage markers can be successfully imported into the genetic background of the susceptible rice varieties, the resistance of the susceptible rice varieties to the black streaked dwarf viral disease is effectively improved and the selection efficiency reaches 92.31 percent. The method is easy to implement, quick and efficient, and is an effective method for improving the resistance to the rice black streaked dwarf viral disease.

Description

technical field [0001] The present invention is aimed at the main resistance QTL of rice black-streaked dwarf disease—— qRBSDV-6 The invention provides a method for improving resistance to black-streaked dwarf disease of rice varieties by using its tightly linked molecular markers for assisted selection, and belongs to the field of molecular genetics. Background technique [0002] Rice black-streaked dwarf disease is a disease mainly caused by the small planthopper (BPH Laodelphax striatellus Fallèn) is a viral disease transmitted as a vector. Infected plants generally show severe dwarfism, dark green leaves, short white stripes on the veins on the back of the leaves, failure to head or neck ears, and small or even deformed ears, which significantly affect economic output. and other symptoms. Because once rice is infected with the virus, it cannot be cured, so it is called rice "cancer". The disease first occurred in China in the 1960s. In recent years, with the change...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 李爱宏戴正元潘存红肖宁余玲李育红张小祥刘广青赵步洪王宝
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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