14results about How to "Accelerate the cultivation process" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the testis size character of Hu sheep as well as a screening method and application of the SNP molecular marker, and belongs to the fields of genetic breeding and molecular biology. The marker is located in an intron region of a Hu sheep chromosome 3 LRRIQ1 gene and is remarkably related to the Hu sheep testis size character, and the accuracy and reliability of the SNP molecular marker are ensured through a scientific and rigorous screening method; according to the marker, Hu sheep individuals related to testis size characters can be rapidly and accurately screened out, the breeding efficiency and accuracy are improved, the breeding process is accelerated, the breeding cost is reduced, and the economic benefits are improved; the genetic diversity is protected, the accuracy of seminal fluid quality evaluation is improved, a powerful tool is provided for Hu sheep breeding work, and important basic data is also provided for researching a genetic mechanism of testis development and functions; the method can also be used as an auxiliary diagnosis tool to assist in identifying potential genetic defects or disease risks and promote progress and development of the breeding technology.

The present invention belongs to the technical field of molecular genetics, and specifically relates to a SNP site related to the fine wool rate of fine wool sheep and its application. The SNP molecular marker described in the present invention is located at the A / T mutation of the 236304249th nucleotide site on chromosome 1 of the international sheep reference genome Oar_v4.0 version. The present invention also provides the use of a reagent for detecting the above-mentioned SNP site in detecting the fine wool rate trait of fine wool sheep or in molecular marker-assisted breeding of fine wool sheep, which has great application value in fine wool sheep variety screening, variety identification or molecular marker-assisted breeding; it is used for early breeding selection, shortening the generation interval, accelerating the breeding process, saving a lot of breeding costs, and has broad market prospects.

The invention relates to the field of aquaculture, in particular to mandarin fish fry domestication feed and application. The feed is specially designed for mandarin fish fries, aims to promote the fries to quickly adapt to artificial feed and improve the survival rate and growth speed of the fries, and is mainly composed of fish meal, mixed algae powder, dried porcine soluble, soybean meal, peanut meal, meat and bone meal, blood meal and the like, and matched with vitamin premix and mineral premix. According to the feed, by optimizing the proportion of protein, fat and carbohydrate, the protein content of the feed is larger than 50%, the fat content of the feed is larger than 10%, the nutritional requirement for rapid growth of fish fries is met, the vitamin premix in the feed contains vitamin A, vitamin E and vitamin C, the disease resistance of the fish fries is effectively improved, and the survival rate of the fish fries is increased. The feed provided by the invention can significantly improve the domestication effect of large-scale mandarin fish fries.

The present invention belongs to the field of genetic engineering technology, and more particularly to a distal enhancer of the ADAMTS1 gene related to the tail type of sheep and its application in the early screening of fat or thin tails of sheep. The distal enhancer of the ADAMTS1 gene of the present invention is located at chromosome 1, 128464792-128465192 of the sheep reference genome ARS‑UI_Ramb_v2.0. By detecting the influence of the distal enhancer on the expression of the ADAMTS1 gene, different tail type traits of sheep can be detected, which can be used for the early selection of sheep with different tail types, improve the accuracy of seed selection, shorten the breeding cycle, speed up the breeding process, and provide a scientific basis for the molecular marker-assisted selection of sheep.

The present invention provides an InDel molecular marker co-segregated with melon pulp thickness and its application. The InDel molecular marker is obtained by amplification of the upstream primer CmFpt-F shown in SEQ ID NO:1 and the downstream primer CmFpt-R shown in SEQ ID NO:2. The molecular marker primer is not only co-segregated with the pulp thickness trait in natural populations, but its amplified band type is also consistent with the pulp thickness phenotype in different breeding materials. The primer pair designed based on the InDel molecular marker and the kit thereof can be used to screen the melon pulp thickness trait at the budding stage or the seedling stage. Therefore, the molecular marker, primer pair, kit, etc. provided by the present invention can be directly used in melon molecular marker-assisted breeding, reduce the production cost of planting large populations, accelerate the breeding process, and improve the breeding efficiency, and have important application value; at the same time, it can improve the accuracy of selection and accelerate the process of variety breeding.

The invention provides a closely linked SSR marker and application thereof in barley helminthosporium leaf spot resistant QTL positioning, and relates to the technical field of molecular marker assisted breeding. The two QTL sites closely linked with the helminthosporium leaf spot resistance character are successfully positioned through the SSR marker technology, the accuracy of the SSR marker is further verified, the positioning work of the barley helminthosporium leaf spot resistance QTL is successfully completed within a short time by utilizing the SSR marker technology, timely and effective support is provided for disease-resistant breeding, and the method has the advantages of being high in practicability and high in practicability. According to the calculated QTL effect, the position of the QTL on the genetic map is determined, fine positioning of the QTL can be achieved, cross breeding is conducted through the screened helminthosporium leaf spot resistant genotype, a new helminthosporium leaf spot resistant barley variety can be more quickly cultivated in combination with a traditional breeding method, and the strategy of the modern genetic technology and the traditional breeding method is combined.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PcWVD2 gene in enhancing the dwarfing ability of pear plants. The CDS (Coding Sequence) of the PcWVD2 gene is as shown in SEQ ID NO. 1. The amino acid sequence coded by the PcWVD2 gene is as shown in SEQ ID NO. 2. The invention reveals that the PcWVD2 gene has the function of regulating and controlling the dwarf character of the pear plant, the dwarf ability of the pear can be obviously enhanced by over-expressing the gene, the dwarf ability shows that the plant height is reduced, the internode is shortened and the root length is shortened, and an important candidate gene is provided for dwarf close planting cultivation of the pear tree. According to the method for enhancing the dwarfing ability of the pear plant, the endogenous dwarfing gene PcWVD2 is directly overexpressed by the pear plant through genetic transformation, the stably inherited dwarfing character can be realized, the dependence on dwarfing rootstock can be gotten rid of, and the cultivation management cost can be reduced.

The application discloses a method for regulating flowering time of sugarcane hybrid parents by using GA3 and application, and is based on a "seesaw" regulation mode of a flowering promotion type gene ScFT4 and a flowering inhibition type gene ScFT1 and response characteristics to exogenous gibberellin GA3, wherein specific concentration GA3 is sprayed at different time nodes before and after swelling of flower buds of the sugarcane, so that the sugarcane hybrid parents can bloom in advance or delay bloom. 5mg / L GA3 is sprayed 60 days before swelling of the flower buds, and the plants bloom at least 6 days in advance; 5mg / L or 50mg / L GA3 is sprayed 30 days before swelling of the flower buds, and the flowering time is delayed by not less than 6 days or 18 days, respectively. The method can effectively solve the problem of non-meeting of flowering time of the sugarcane hybrid parents, significantly improve the efficiency of sugarcane hybrid breeding, and has important application prospect.

This invention discloses a molecular marker InDel05 related to soybean oil content, detection primers, and their applications. The molecular marker InDel05 is located at the physical location 41854660-41854682 on soybean chromosome 5, and its nucleotide sequence is shown in SEQ ID No. 1. Based on this, detection primers for the molecular marker InDel05 were designed. The length difference of the amplification products is detected by PCR amplification. Soybean materials without the inserted fragment in the PCR amplification product are high-oil-content soybeans, while those with the inserted fragment are low-oil-content soybeans, thus achieving the identification of soybean oil content phenotypes with an accuracy of up to 87.5%. The molecular marker InDel05 and detection primers provided by this invention can be used for rapid, accurate, and efficient screening of high-oil-content soybean germplasm, significantly improving the efficiency of marker-assisted selection breeding and accelerating the breeding process of high-oil-content soybean varieties.

The present invention relates to an induction method for distant hybridization triploid grouper, and belongs to the technical field of genetic breeding of grouper. The present invention adopts hydrostatic pressure combined with chemical drug induction method to cultivate triploid grouper. The method is to obtain fertilized eggs of female brown-spotted grouper and male blue-bodied big-spotted grouper, record the starting time of fertilization, put the fertilized eggs into a hydrostatic pressure instrument for hydrostatic shock treatment, and inject natural seawater containing 6-dimethylaminopurine into the hydrostatic pressure instrument in advance. After the hydrostatic pressure treatment, the fertilized eggs are moved into a 6-DMAP solution to continue drug induction, and finally the fertilized eggs are transferred to aquaculture water for hatching. The present invention obtains the induction parameters of distant hybridization grouper triploid for the first time. The method is simple to operate, short in processing time, low in damage to fertilized eggs, and the induction rate is more than 60%.

This invention discloses a biomarker combination for rapid growth in high-density cultured grass carp based on plasma exosomal miRNAs, consisting of three miRNAs: dre-miR-146b, efu-miR-143, and spu-miR-133. The invention also discloses a screening method for this biomarker. Furthermore, this invention discloses a method for identifying fast-growing grass carp species in high-density culture. This invention has the advantage of enabling early and accurate identification of individuals with rapid growth potential in high-density grass carp culture environments.

The present invention belongs to the technical field of molecular genetics, and specifically relates to a SNP site related to the fine wool rate of fine wool sheep and its application. The SNP molecular marker described in the present invention is located at the A / G mutation of the 60455332nd nucleotide site on chromosome 5 of the international sheep reference genome Oar_v4.0 version. The present invention also provides the use of a reagent for detecting the above-mentioned SNP site in detecting the fine wool rate trait of fine wool sheep or in molecular marker-assisted breeding of fine wool sheep, which has great application value in fine wool sheep variety screening, variety identification or molecular marker-assisted breeding; it is used for early breeding selection, shortening the generation interval, accelerating the breeding process, saving a lot of breeding costs, and has broad market prospects.

The invention provides a molecular marker related to regulation and control of Chinese rose flower aging and application of the molecular marker, and belongs to the technical field of molecular markers. The primer group is used for identifying the genotype of the InDel molecular marker related to the life of the Chinese rose flowers; wherein the InDel molecular marker related to the identification of the life of the Chinese rose flower is located at a 75886684 position of a Chr2A chromosome of a 'Sangmansa' Chinese rose genome; the position of the molecular marker is determined on the basis of a modern Chinese rose 'Sammansa' reference genome. Based on the InDel variation, the invention develops a specific molecular marker, a detection primer and a typing method based on a KASP technology, Chinese rose plants with long-flowering-period potential can be rapidly and accurately screened in a seedling stage, an efficient tool is provided for molecular marker assisted breeding, and important theoretical and application values are achieved.

The invention discloses a chimeric gene for improving genetic transformation efficiency of potatoes as well as a vector and application of the chimeric gene, and belongs to the technical field of plant genetic engineering. According to the chimeric gene capable of improving the genetic transformation efficiency of the potatoes as well as the vector and the application of the chimeric gene, GRF4 and GIF1 genes are identified from the potatoes, a StGRF4-GIF1 chimeric gene sequence is synthesized, and the potato StGRF4-GIF1 chimeric gene overexpression vector is constructed. Potato 'E109' C9701 'RH' S15 'is taken as a material, a potato tissue culture seedling stem segment is subjected to genetic transformation by an agrobacterium-mediated method, the bud regeneration rate of an obtained transformed potato StGRF4-GIF1 chimeric gene overexpression vector explant is higher than that of a control, the genetic transformation efficiency of potatoes is improved, and it is proved that the StGRF4-GIF1 chimeric gene has a promoting effect on potato regeneration.