The invention provides a formula of an inducing medium for tomato anther culture breeding. According to the formula, the inducing medium is prepared from KNO3, NH4NO3, KH2PO4, MgSO4.H2O, CaCl2.2H2O, Na2-EDTA, FeSO4.7H2O, MnSO4.H2O, ZnSO4.7H2O, H3BO3, KI, CuSO4.5H2O, CoCl.6H2O, NaMoO4.2H2O, AgNO3, inositol, vitamin B1, vitamin B6, nicotinic acid, glycine, folic acid, cyclic guanosine monophosphate, maltose, cane sugar, phytagel, 2,4-D, kinetin, complex phthalein nucleic acid, biotin, vitamin C, active carbon and protein hydrolysate at a proper proportion. The medium has the advantage of efficiently inducing tomato anther to generate pollen calli, and can significantly improve the tomato anther culture efficiency and quicken the tomato improved variety breeding progress.