51results about How to "Accelerate the cultivation process" patented technology

The invention discloses a method for assisting the breeding of pericarp anthocyanin-synthesized fresh corn inbred lines based on ligule color. The method is in the breeding process of pericarp anthocyanin-synthesized fresh corn inbred lines Among them, according to the color traits of the ligule, the pericarp anthocyanin-synthesized fresh corn inbred line was selected. Hybrid selection, F1 generation self-bred seedlings, in the F2 generation, according to the color traits of the ligule, select individual plants with purple ligules for self-bred seedlings, plant according to panicle rows, and select individual plants with good phenotype traits , several generations of continuous selfing selection, and stable hereditary pericarp anthocyanin synthetic maize inbred lines can be obtained. Compared with the prior art, the method can speed up the breeding process of the pericarp anthocyanin-synthesized fresh corn inbred line, reduce the workload, and improve the breeding efficiency of the colored corn varieties.

The invention provides a rooting substrate of Moringa oleifera, a rooting method and application thereof, and belongs to the technical field of Moringa oleifera cultivation. The rooting substrate of Moringa oleifera in the present invention is sawdust, and the water content of the sawdust is 50-60%. The rooting matrix of the invention has a wide range of sources and low cost, and the rooting matrix is ​​used for rooting Moringa oleifera branches, and the rooting rate can reach 100% in about 30 days. When the rooting matrix is ​​used for rooting Moringa oleifera, the method is simple, easy to operate, and small in investment, and is not only suitable for scientific research units to do related research topics, but also suitable for large-scale factory cultivation.

The invention discloses a molecular marker linked with cotton fiber length QTL / major genetic loci qFL-C13-1 and qFL-C22-1. The qFL-C13-1 and the qFL-C22-1 are positioned on a chromosome C13 and a chromosome C22 respectively. A molecular marker tightly linked with the qFL-C13-1 is CGR5242100, and a molecular marker tightly linked with the qFL-C22-1 is NAU2977150. The SSR markers linked with the cotton fiber length QTL / the major genetic loci are adopted for performing auxiliary selection, so that the cotton fiber length breeding efficiency can be improved.

The invention provides an SNP (Single Nucleotide Polymorphism) marker relevant to the growth rate of schizothorax prenanti and application thereof. The SNP marker comprises an SNP1 marker and an SNP2 marker; nucleotide sequences are as shown by SEQ ID NO. 1 and SEQ ID NO. 2 in sequence. When the genotypes of SNP1 and SNP2 loci are both homozygous CC, the growth rate of the individual of a to-be-detected schizothorax prenanti is quicker. Individuals of which the genotypes at the SNP1 and SNP2 loci are both the CC are selected for hybridization; the genotypes, at the SNP1 and SNP2 loci, of individuals of a later generation are all the homozygous CC; the individuals of the later generation are all quick growing type ones. The SNP marker provided by the invention is closely relevant to the growth rate of the schizothorax prenanti; a parent can be selected according to a breeding requirement at a fry culture early stage; the marker provided by the invention is utilized to carry out assistant breeding; further, the culture progress of the excellent variety of the schizothorax prenanti is quickened.

The SNP molecular marker related to corn row kernel number and its application belong to the technical field of crop genetic breeding molecular marker application technology, and the invention specifically relates to the SNP molecular marker and application related to two row kernel number traits on the No. 5 chromosome of corn. One of the SNP molecular markers is located in the intron region of gene Zm00001d018060, and the other SNP molecular marker is located in the coding region of gene Zm00001d013521. Both SNP molecular markers are obtained from genome-wide association analysis of maize inbred populations. The acid sequences are respectively shown in: SEQIDN01 and SEQIDN02. The SNP molecular marker of the present invention can be used for auxiliary selection of the trait of grain number in a row in the process of corn breeding, which can improve the accuracy of selection and speed up the process of cultivating new varieties.

The invention discloses a molecular marker of the melon female flower regulation gene g and its application. The primer pair of the present invention is a primer pair capable of amplifying a target sequence; the target sequence is the following 1) or 2): 1) a single-stranded DNA molecule shown in sequence 3 of the sequence listing; 2) sequence 3 is passed through one or A DNA molecule having the same function as sequence 7 with several nucleotide substitutions and / or deletions and / or additions. Utilize the molecular markers provided by the present invention and their corresponding primers to carry out polymerase chain reaction on the total DNA of the melon genome, and after electrophoresis detection of the amplified products, it can be judged whether the single plant to be detected carries the g gene, and the band specificity is good , the result is highly accurate, and the detection is not affected by the environment and genetic background. The invention can screen and identify the female flower regulating gene g in a large amount of melon germplasm resources, can be used for the cultivation of all-female lines of the melon, and can be applied in molecular marker-assisted selection breeding and gene aggregation breeding.

The invention relates to a cultivation method of a Chlamys nobilis new strain enriched with DNA (Deoxyribonucleic Acid) and an application thereof. The cultivation method comprises the following steps of selecting seeds, cultivating an F1 generation genealogy and cultivating an F2 generation new strain; and carrying out two generations of directional breeding to cultivate the Chlamys nobilis new strain enriched with the DNA. The invention further provides the application of the cultivation method in the cultivation of other shellfish new varieties. According to the cultivation method, the shellfish new strain can be cultured from the aspects of sensing, color and cluster, and nutrition; the cultivated Chlamys nobilis new strain has beautiful attractive color and cluster; a shellfish, an adductor muscle and a pallium are orange; in-vivo tissues are enriched with the DNA; and therefore, the Chlamys nobilis new strain has obvious economic value, health-care value and medical value, and has a wide market prospect.