5results about How to "Accelerate the cultivation process" patented technology

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PcWVD2 gene in enhancing the dwarfing ability of pear plants. The CDS (Coding Sequence) of the PcWVD2 gene is as shown in SEQ ID NO. 1. The amino acid sequence coded by the PcWVD2 gene is as shown in SEQ ID NO. 2. The invention reveals that the PcWVD2 gene has the function of regulating and controlling the dwarf character of the pear plant, the dwarf ability of the pear can be obviously enhanced by over-expressing the gene, the dwarf ability shows that the plant height is reduced, the internode is shortened and the root length is shortened, and an important candidate gene is provided for dwarf close planting cultivation of the pear tree. According to the method for enhancing the dwarfing ability of the pear plant, the endogenous dwarfing gene PcWVD2 is directly overexpressed by the pear plant through genetic transformation, the stably inherited dwarfing character can be realized, the dependence on dwarfing rootstock can be gotten rid of, and the cultivation management cost can be reduced.

The application discloses a method for regulating flowering time of sugarcane hybrid parents by using GA3 and application, and is based on a "seesaw" regulation mode of a flowering promotion type gene ScFT4 and a flowering inhibition type gene ScFT1 and response characteristics to exogenous gibberellin GA3, wherein specific concentration GA3 is sprayed at different time nodes before and after swelling of flower buds of the sugarcane, so that the sugarcane hybrid parents can bloom in advance or delay bloom. 5mg / L GA3 is sprayed 60 days before swelling of the flower buds, and the plants bloom at least 6 days in advance; 5mg / L or 50mg / L GA3 is sprayed 30 days before swelling of the flower buds, and the flowering time is delayed by not less than 6 days or 18 days, respectively. The method can effectively solve the problem of non-meeting of flowering time of the sugarcane hybrid parents, significantly improve the efficiency of sugarcane hybrid breeding, and has important application prospect.

This invention discloses a molecular marker InDel05 related to soybean oil content, detection primers, and their applications. The molecular marker InDel05 is located at the physical location 41854660-41854682 on soybean chromosome 5, and its nucleotide sequence is shown in SEQ ID No. 1. Based on this, detection primers for the molecular marker InDel05 were designed. The length difference of the amplification products is detected by PCR amplification. Soybean materials without the inserted fragment in the PCR amplification product are high-oil-content soybeans, while those with the inserted fragment are low-oil-content soybeans, thus achieving the identification of soybean oil content phenotypes with an accuracy of up to 87.5%. The molecular marker InDel05 and detection primers provided by this invention can be used for rapid, accurate, and efficient screening of high-oil-content soybean germplasm, significantly improving the efficiency of marker-assisted selection breeding and accelerating the breeding process of high-oil-content soybean varieties.

This invention discloses a biomarker combination for rapid growth in high-density cultured grass carp based on plasma exosomal miRNAs, consisting of three miRNAs: dre-miR-146b, efu-miR-143, and spu-miR-133. The invention also discloses a screening method for this biomarker. Furthermore, this invention discloses a method for identifying fast-growing grass carp species in high-density culture. This invention has the advantage of enabling early and accurate identification of individuals with rapid growth potential in high-density grass carp culture environments.

The invention provides a molecular marker related to regulation and control of Chinese rose flower aging and application of the molecular marker, and belongs to the technical field of molecular markers. The primer group is used for identifying the genotype of the InDel molecular marker related to the life of the Chinese rose flowers; wherein the InDel molecular marker related to the identification of the life of the Chinese rose flower is located at a 75886684 position of a Chr2A chromosome of a 'Sangmansa' Chinese rose genome; the position of the molecular marker is determined on the basis of a modern Chinese rose 'Sammansa' reference genome. Based on the InDel variation, the invention develops a specific molecular marker, a detection primer and a typing method based on a KASP technology, Chinese rose plants with long-flowering-period potential can be rapidly and accurately screened in a seedling stage, an efficient tool is provided for molecular marker assisted breeding, and important theoretical and application values are achieved.