51results about How to "Accelerate the cultivation process" patented technology

The invention discloses a method for breeding a new golden-shell crassostrea gigas strain. The method is characterized by including the operating steps of (1) breeding the first generation: selecting wild parent crassostrea gigas with golden left shells, and creating a plurality of families without genetic relationships; (2) breeding the second generation: selecting the families with the obvious golden-shell characters from the created first-generation families, and screening out the crassostrea gigas with the golden left shells and the golden right shells to carry out inner-family inbreeding; (3) breeding the third generation: carrying out inter-family hybridization on the families, with the left shells and the right shells being in the golden characters, obtained from the created second-generation families; (4) breeding the fourth generation: carrying out stability verification on the families with the left shells and the right shells being in the golden characters. According to the method, the breeding process of the new crassostrea gigas shell-color strain is accelerated, and new products of bred shellfishes are increased; the product additional value is increased through the color characters, the breeding yield is improved through the growth characters, incomes of breeding enterprises and farmers are increased, and the market prospects are broad.

The invention relates to the field of molecular breeding, and particularly relates to a fiber strength related molecular mark from gossypium barbadense and application thereof. The molecular mark is HAU0883125 and TMB1125220, wherein the HAU0883125 is closely linked with the site qFS-C14-1 of cotton fiber strength QTL, and the TMB1125220 is closely linked with the site qFS-C20-1 of cotton fiber strength QTL; and the qFS-C14-1 is located on chromosome C14, and the qFS-C20-1 is located on chromosome C20. The molecular mark can be used for improving the selection efficiency of fiber strength, overcoming the defect in existing breeding technology for fiber quality identification and accelerating the culturing progress of high-quality new varieties.

The invention discloses a molecular marker linked with cotton fiber length QTL / major genetic loci qFL-C13-1 and qFL-C22-1. The qFL-C13-1 and the qFL-C22-1 are positioned on a chromosome C13 and a chromosome C22 respectively. A molecular marker tightly linked with the qFL-C13-1 is CGR5242100, and a molecular marker tightly linked with the qFL-C22-1 is NAU2977150. The SSR markers linked with the cotton fiber length QTL / the major genetic loci are adopted for performing auxiliary selection, so that the cotton fiber length breeding efficiency can be improved.

The invention provides close linkage simple sequence repeat (SSR) molecular markers of a main effect quantitative trait locus (QTL), namely qRBSDV-6 for resistance to rice black streaked dwarf viral disease, and a method for improving the resistance of susceptible rice varieties through marker assistant selection. For derived line segregating population obtained by hybridizing and back-crossing Minghui 63 serving as a resistance source and a susceptible receptor parent, genotypes of the close linkage markers RM7158 and RM587 on two sides of the main effect quantitative trait locus (QTL), namely qRBSDV-6 for resistance to black streaked dwarf viral disease on the short arm of the sixth chromosome of Minghui 63 are detected, the close linkage markers can be successfully imported into the genetic background of the susceptible rice varieties, the resistance of the susceptible rice varieties to the black streaked dwarf viral disease is effectively improved and the selection efficiency reaches 92.31 percent. The method is easy to implement, quick and efficient, and is an effective method for improving the resistance to the rice black streaked dwarf viral disease.

The invention discloses a molecular marker linked with cotton fiber strength QTL / major genetic loci qFS-C12-1 and qFS-C24-1, The qFS-C12-1 and the qFS-C24-1 are positioned on a chromosome C12 and a chromosome C24 respectively. A molecular marker tightly linked with the qFS-C12-1 is HAU1361240, and a molecular marker tightly linked with the qFS-C24-1 is PGML03854240. The SSR markers linked with the cotton fiber strength QTL / the major genetic loci are adopted for performing auxiliary selection, so that the cotton fiber strength breeding efficiency can be improved.

The invention discloses a genetic transformation and regeneration method for eucalyptus. The method comprises the following steps: firstly, preparing infectious agrobacterium carrying an exogenous gene; then, preparing a sprout pile of eucalyptus as an explant; then, co-culturing the sprout pile of eucalyptus and the agrobacterium carrying the exogenous gene for genetic transformation and regeneration; and finally, carrying out selective rooting culture and transplanting on the generated sprout bud to obtain a planed transformation plant. By using the sprout pile of a eucalyptus seedling as the explant which is co-cultured and converted with the agrobacterium carrying an the gene, the generated sprout bud is screened and cultured in a rooting culture medium, and the sprout bud rooted is transplanted to obtain the planed transformation plant. By adopting the method for transgenetic operation of eucalyptus, a positive transformation plant can be obtained within about 50 days. The method has the characteristics of short period, simple operation, low cost, wide applicability and the like. The invention provides a quick transgenetic method for molecular breeding and batched transgenetic operation of eucalyptus, thereby accelerating the progress of cultivation of novel varieties of eucalyptus.

The invention relates to the technical field of molecular identification of fish, in particular to an SNP marker associated with vibrio harveyi disease of large yellow croaker and primers and applications thereof, wherein the nucleotide sequence of the SNP marker is shown as SEQ ID NO. 1, and the nucleotide sequence starts from base G or T at position 343 from 5 'end. The invention overcomes the problem that there is no SNP marker which can be used for the selection and breeding of disease resistance related traits of large yellow croaker in the prior art, and at the SNP site of the invention,the probability of the large yellow croaker with the genotype of GT to be infected with Vibrio harveyi is significantly higher than that of the homozygous GG genotype individual. Therefore, by detecting the SNP of Pseudosciaena crocea, it can be effectively determined whether it is susceptible to Vibrio harveyi infection. Elimination of GT genotype individual in parent breeding is favorable for improve that ability of offspring to resist infection of Vibrio harveyi, and the marker of the invention is used for auxiliary breed, thereby accelerating the cultivation process of disease-resistant varieties of large yellow croaker.

The invention discloses a molecular marker of a female flower regulating gene g of a muskmelon and an application thereof. A prim pair of the invention is a primer pair capable of amplifying a targetsequence; the target sequence is 1) or 2) as follows: 1) a single-stranded DNA molecule shown in SEQ ID NO:3 of a sequence table; 2) a DNA molecule of SEQ ID NO:3 which is substitute and / or deleted and / or added by one or several nucleotides and has the same function as SEQ ID NO:7. The invention utilizes the molecular marker and the corresponding primer to carry out polymerase chain reaction on the total DNA of the genome of the muskmelon, and after the amplification product is subjected to electrophoresis detection, it can be judged whether the individual plant to be detected carries the g gene, the band specificity is good, the accuracy rate of the result is high, and the detection is not influenced by the environment and the genetic background. The invention can be used for screening and identifying female flower regulating gene g in a large number of muskmelon germplasm resources, can be used for breeding muskmelon whole female line, and can be used for molecular marker assisted selection breeding and gene polymerization breeding.

The invention provides a formula of an inducing medium for tomato anther culture breeding. According to the formula, the inducing medium is prepared from KNO3, NH4NO3, KH2PO4, MgSO4.H2O, CaCl2.2H2O, Na2-EDTA, FeSO4.7H2O, MnSO4.H2O, ZnSO4.7H2O, H3BO3, KI, CuSO4.5H2O, CoCl.6H2O, NaMoO4.2H2O, AgNO3, inositol, vitamin B1, vitamin B6, nicotinic acid, glycine, folic acid, cyclic guanosine monophosphate, maltose, cane sugar, phytagel, 2,4-D, kinetin, complex phthalein nucleic acid, biotin, vitamin C, active carbon and protein hydrolysate at a proper proportion. The medium has the advantage of efficiently inducing tomato anther to generate pollen calli, and can significantly improve the tomato anther culture efficiency and quicken the tomato improved variety breeding progress.

The invention discloses a wastewater treatment device, particularly a biological treatment device of high-ammonia-nitrogen wastewater, which relates to a biological treatment device of wastewater. The invention solves the problems that when high-ammonia-nitrogen wastewater is treated by using existing conventional wastewater treatment processes, the removal effect is not good, the reaction time is long, the sludge production is large, the operation cost is high, the occupied land is large, and the sludge concentration is increased so as to affect the precipitation effect of secondary precipitation. According to the invention, floc sludge is arranged in a lower reaction cylinder, an upper reaction area and a lower reaction area are connected by the cylinder, a mixed stirring device is arranged in the reaction cylinder, the upper reaction area and the lower reaction area are respectively provided with a water outlet pipe, and the upper reaction area and the lower reaction area are connected by a hose. The device disclosed by the invention has the advantages of good ammonia-nitrogen removal effect, low investment and operation costs, small equipment occupied land, simple structure, and the like.

The invention provides a mutation breeding method for Chinese pennisetum, and belongs to the technical field of seed selection of new-variety plants. The method comprises the steps that Chinese pennisetum seeds are disinfected, soaked, inoculated into an EMS solution with the volume percent content of 0.4%-1.2% and induced for 4-6 hours under the indoor-temperature dark condition, the induced seeds are planted, and after the steps of seed germination, seedling culturing in a greenhouse, transplanting into large land and the like are conducted, in combination with field investigation, good single plants are screened out; a tillering cuttage method is adopted for asexual propagation expansion for the selected single plants, so that good characters of the selected single plants are fixed. Through continuous 4-6 generation asexual propagation expansion and three years of variety comparison tests, stable and consistent new-strain Chinese pennisetum can be cultured. The method is simple andconvenient to implement, mature, stable and easy to popularize and accelerates the process of culturing the new-variety ornamental Chinese pennisetum.

The invention discloses a breeding method for a hypoxic resistant pseudobagrus ussuriensis pedigree. The method includes the steps of parent selection, spawning induction, insemination, incubation, fry breeding, hypoxia stress, survival rate calculation and the like. By selecting parents with well-developed gonads, a good oxytocic effect can be achieved under the action of oxytocin of appropriate proportions and doses; by conducting egg squeezing, insemination, egg laying, incubation, fry breeding and the other steps on each female fish individually, a pseudobagrus ussuriensis pedigree is built; finally, by effective hypoxia stress treatment, the hypoxic resistant pedigree is bred. Through the technologies of building the pseudobagrus ussuriensis pedigree and breeding the hypoxic resistant pedigree, technology support can be provided for artificial selection of pseudobagrus ussuriensis based on several pedigrees, and meanwhile, it is of great significance for speeding up the process of breeding hypoxic resistant pseudobagrus ussuriensis new varieties.

The invention discloses application of a KASP molecular marker related to wheat grain length. One technical scheme protected by the invention is application of a composition for detecting polymorphism or genotype (namely allele) of a QTLqGL3B.1 site in a wheat genome in preparation of a product for identifying or assisting in identifying grain length. The composition for detecting the polymorphism and genotype of the QTLqGL3B.1 site can be combined with other substances (such as substances for detecting the single nucleotide polymorphism or genotype of other molecular markers related to the length of wheat grains) to prepare products for high-throughput identification of long-grain wheat varieties.

The invention provides an SNP marker related to the growth rate of leiocassis longirostris and application of the SNP marker. The SNP marker comprises an SNP1 marker and an SNP2 marker, and nucleotide sequences of the SNP1 marker and the SNP2 marker are shown as SEQ ID NO.1 and SEQ ID NO.2 in sequence. When the genotype of the SNP1 site is mutated homozygous TT and the genotype of the SNP2 site is mutated homozygous GG, the growth speed of the individual leiocassis longirostris to be detected is higher. The individuals with the genotype of TT at the SNP1 site and the individuals with the genotype of GG at the SNP2 site are selected to be hybridized, and the offspring individuals are of a rapid growth type. The SNP marker disclosed by the invention is closely related to the growth speed of the leiocassis longirostris, parents can be selected according to breeding requirements in the early stage of fry culture, and the marker disclosed by the invention is used for assisted breeding, so that the culture process of good varieties of the leiocassis longirostris is accelerated.

The invention aims to provide a gene chip for breeding a cynoglossus semilaevis disease-resistant improved variety, solves the problem that a gene chip is lacked in cynoglossus semilaevis disease-resistant improved variety breeding, overcomes the defects of a traditional breeding technology, and provides a novel molecular breeding method for breeding a disease-resistant high-yield high-quality improved variety. The gene chip can be used for SNP typing of cynoglossus semilaevis at the whole genome level, calculating the genetic effect of each SNP site, estimating the genome breeding value (GEBV) of an individual, and screening fishes with high disease resistance according to the GEBV of the individual; and the survival rate of the offspring can be obviously improved by utilizing the optimized parent fishes to breed the offspring. Therefore, by utilizing the gene chip, the breeding process of the cynoglossus semilaevis disease-resistant improved variety can be accelerated, the breeding period is shortened, and an efficient molecular breeding technical means is provided for breeding of the cynoglossus semilaevis disease-resistant improved variety.

The invention discloses a molecular marker-assisted breeding method of a new variety for rapid growth of trachinotus ovatus. According to the method, by efficiently integrating technologies such as individual phenotype value selection, microsatellite molecular marker, additive genetic correlation matrix, linear hybrid model, quantity genetics, individual breeding value selection, gender specific molecular marker, inter-individual genetic distance analysis, group mating scheme and the like, the problems of slow fry growth, low survival rate, poor stress resistance and disease resistance, germplasm degeneration and the like caused by difficulty in candidate parent genetic evaluation and optimal selection and blind inbreeding due to unclear genetic relationship and sex in existing trachinotus ovatus genetic breeding work can be effectively solved.

The invention discloses a creation method of a special soybean protein germplasm for processing. The method comprises steps as follows: abundant seed main storage protein 7S and 11S component subunit content variation genes in soybean germplasm resources are utilized for carrying out identification and selection by means of pyramiding breeding, crossbreeding and the like in combination with an SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method and a molecular identification means, and firstly, germplasms only containing a 7S component and germplasms only containing a 11S component are created; then hybridized combinations with a series of germplasms highly containing the 7S component and a series of germplasms highly containing the 11S components obtained by screening are configured respectively, a series of materials only containing and highly containing the 7S component and a series of materials only containing and highly containing the 11S component are obtained through selection and stabilization, and strains are cultivated; and nutritional characteristics, functionality and processing characteristics are evaluated respectively, and the strains are classified into special soybean materials suitable for different processed products, and therefore, high-quality special raw materials are provided for soybean processing and utilization.

The invention belongs to the technical field of brassica napus germplasm improvement, and discloses a method for synthesizing clubroot resistant brassica napus through pyramid breeding and a biotechnology. Through distant hybridization, a disease-resistant descendant strain of a clubroot resistant Chinese cabbage variety namely Wendingchunlei and clubroot resistant mid-maturity Chinese kale are used as resistant gene sources of clubroot; double 11 in susceptible brassica napus varieties is used as a recurrent parent, a real hybrid obtained through distant hybridization is used as a female parent, backcross is performed, plants containing Chinese cabbage and Chinese kale resistance are screened through a molecular marker IP13-2 and a molecular marker KASP1 resisting the clubroot in the Chinese kale, SSR markers covering complete genomes are used for performing background selection on descendant plants, and resistant plants having highest similarity to the recurrent parent are selected.According to the method disclosed by the invention, the molecular markers are used for performing auxiliary selection and acceleration of a breeding process of clubroot resistant brassica napus strains, a plurality of clubroot resistant genes are polymerized, the resistance of the brassica napus to the clubroot is increased, and the breeding process is accelerated.

The invention discloses a corychophramus violaceua embryoid and plant cultivation method. The method comprises the following steps of corychophramus violaceua planting, microspore culture, and plant regeneration and transplanting. In the microspore culture stage, the heat-induced culture time and the activated carbon concentration influence the yield of embryoids, wherein ratio (mg / mL) of the addition amount of the activated carbon to the massive volume of a microspore NLN-13 suspension is 1:4-3:4, and the heat-induced culture condition specifically includes that dark culture is carried out for 1-7 days at the temperature of 32-35 DEG C. The method can be used for rapidly obtaining a large number of corychophramus violaceua embryoids and genetically stable regenerated plants, and is beneficial for accelerating the process of breeding a new variety of corychophramus violaceua.

The invention discloses a novel creating method for processing serial high-quality protein into special soybean germplasm. Chinese soybean germplasm resources have rich main seed storage protein (7S and 11S components) subunit deleted genes, the genes are identified and selected by means of pyramiding breeding and cross breeding and by an SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) method, 7S component protein subunit deleted germplasm, 11S component protein subunit deleted germplasm and 7S+11S component protein subunit all-deleted germplasm are firstly created, the specific germplasm is further respectively crossly combined with current Chinese major varieties, various 7S and 11S component protein subunit single-deleted, double-deleted, triple-deleted, quadruple-deleted, quintuple-deleted, sextuple-deleted, septuple-deleted and all-deleted materials with genetic stability are finally obtained by means of selection and stabilization and are further cultivated into strains, accordingly, the cultivating process of Chinese protein processed special soybean varieties is accelerated, and high-quality raw materials are provided for Chinese soybean protein processing and utilization.

The invention provides a protein VaPBP2-L for enhancing drought resistance of plants as well as a coding gene and application of the protein VaPBP2-L. A protein VaPBP2-L gene is separated by taking red-bean germinated seeds as materials, an amino acid sequence of the protein VaPBP2-L gene is shown as SEQ ID NO.1, and after a virus expression vector is constructed by using the gene and is excessively expressed and transformed into tobacco, the drought resistance of the plants can be remarkably improved; and various plant expression vectors are constructed by utilizing the protein VaPBP2-L gene, the protein VaPBP2-L gene can be widely applied to cultivation of new drought-resistant varieties of transgenic plants and crops, is applied to drought-resistant breeding of plants as a drought-resistant genetic resource, and a cultivation process of the new drought-resistant varieties (lines) of the crops and the plants is promoted.

The invention belongs to the field of crop genetics and breeding, and provides a wheat dormancy duration candidate gene TaCNGC-2A. A wheat plant pre-harvest sprouting resistance duration judgment functional marker, namely CAPS marker CNG2A, is obtained by using the candidate gene, the functional marker has A / T base variation at the TaCNGC-2A gene promoter-313 position, and the marker sequence is shown as SEQ ID NO.2. A corresponding marker primer is designed according to the marker sequence, and the length of an amplification product is 813 bp. The amplification product can be directly detected in 1.5% agarose gel electrophoresis after being digested at 37 DEG C for 3 h by a FokI restriction enzyme (GGATGN9 / ). The CNG2A-a allelic variation cannot be digested, while the CNG2A-b allelic variation can be digested, and the digestion products are 584 bp and 229 bp respectively; besides, plants carrying the CNG2A-b allelic variation have a longer pre-harvest sprouting resistance duration than plants carrying CNG2A-a. The marker can be directly applied to molecular-marker-assisted wheat pre-harvest sprouting resistance breeding selection and also can accelerate the cultivation process ofpre-harvest sprouting resistant varieties.

The invention discloses a reagent for detecting SNP locus genotype related to grouper nervous necrosis virus resistance. The invention discloses an SNP (Single Nucleotide Polymorphism) site related tonervous necrosis virus resistance. The death probability of groupers with the SNP site genotype of CT (Computed Tomography) infected with nervous necrosis viruses is obviously lower than that of individuals with the genotype of CC or TT. By detecting the genotype of the SNP of the epinephelus akaara, whether the epinephelus akaara has nervous necrosis virus resistance can be effectively determined. The reagent is advantaged in that the SNP marker provided by the invention is closely related to the resistance of epinephelus akaara to nervous necrosis viruses, selection of individuals with CT genotypes in parent breeding is beneficial to improving the nervous necrosis disease resistance of offspring, and the marker provided by the invention is used for auxiliary breeding, so the culture process of excellent disease-resistant varieties of epinephelus akaara can be accelerated.

The invention discloses a molecular marker linked with cotton fiber length QTL / major genetic loci qFL-C13-1 and qFL-C22-1. The qFL-C13-1 and the qFL-C22-1 are positioned on a chromosome C13 and a chromosome C22 respectively. A molecular marker tightly linked with the qFL-C13-1 is CGR5242100, and a molecular marker tightly linked with the qFL-C22-1 is NAU2977150. The SSR markers linked with the cotton fiber length QTL / the major genetic loci are adopted for performing auxiliary selection, so that the cotton fiber length breeding efficiency can be improved.

The SNP molecular marker related to corn row kernel number and its application belong to the technical field of crop genetic breeding molecular marker application technology, and the invention specifically relates to the SNP molecular marker and application related to two row kernel number traits on the No. 5 chromosome of corn. One of the SNP molecular markers is located in the intron region of gene Zm00001d018060, and the other SNP molecular marker is located in the coding region of gene Zm00001d013521. Both SNP molecular markers are obtained from genome-wide association analysis of maize inbred populations. The acid sequences are respectively shown in: SEQIDN01 and SEQIDN02. The SNP molecular marker of the present invention can be used for auxiliary selection of the trait of grain number in a row in the process of corn breeding, which can improve the accuracy of selection and speed up the process of cultivating new varieties.

The invention discloses a molecular marker of the melon female flower regulation gene g and its application. The primer pair of the present invention is a primer pair capable of amplifying a target sequence; the target sequence is the following 1) or 2): 1) a single-stranded DNA molecule shown in sequence 3 of the sequence listing; 2) sequence 3 is passed through one or A DNA molecule having the same function as sequence 7 with several nucleotide substitutions and / or deletions and / or additions. Utilize the molecular markers provided by the present invention and their corresponding primers to carry out polymerase chain reaction on the total DNA of the melon genome, and after electrophoresis detection of the amplified products, it can be judged whether the single plant to be detected carries the g gene, and the band specificity is good , the result is highly accurate, and the detection is not affected by the environment and genetic background. The invention can screen and identify the female flower regulating gene g in a large amount of melon germplasm resources, can be used for the cultivation of all-female lines of the melon, and can be applied in molecular marker-assisted selection breeding and gene aggregation breeding.