2results about How to "Accelerate the cultivation process" patented technology

This invention discloses a molecular marker InDel05 related to soybean oil content, detection primers, and their applications. The molecular marker InDel05 is located at the physical location 41854660-41854682 on soybean chromosome 5, and its nucleotide sequence is shown in SEQ ID No. 1. Based on this, detection primers for the molecular marker InDel05 were designed. The length difference of the amplification products is detected by PCR amplification. Soybean materials without the inserted fragment in the PCR amplification product are high-oil-content soybeans, while those with the inserted fragment are low-oil-content soybeans, thus achieving the identification of soybean oil content phenotypes with an accuracy of up to 87.5%. The molecular marker InDel05 and detection primers provided by this invention can be used for rapid, accurate, and efficient screening of high-oil-content soybean germplasm, significantly improving the efficiency of marker-assisted selection breeding and accelerating the breeding process of high-oil-content soybean varieties.

This invention discloses a biomarker combination for rapid growth in high-density cultured grass carp based on plasma exosomal miRNAs, consisting of three miRNAs: dre-miR-146b, efu-miR-143, and spu-miR-133. The invention also discloses a screening method for this biomarker. Furthermore, this invention discloses a method for identifying fast-growing grass carp species in high-density culture. This invention has the advantage of enabling early and accurate identification of individuals with rapid growth potential in high-density grass carp culture environments.