Molecular mark method for rice anti-leaf drop streak site

A technology of rice stripes and molecular markers, applied in the field of molecular genetics, can solve the problems of environmental pollution and poor results, and achieve the effects of not being affected by the environment, convenient identification, and clear positions

Inactive Publication Date: 2007-01-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, the production is mainly combined with the adjustment of farming system and the improvement of cultivation technology, and the control of insects and diseases at the right time, but the effect is not good, and it pollutes the environment

Method used

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  • Molecular mark method for rice anti-leaf drop streak site
  • Molecular mark method for rice anti-leaf drop streak site

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Embodiment Construction

[0024] The detailed description of the present invention is as follows:

[0025] Studies have shown that there is a major gene locus that controls the stable expression of stripe leaf blight resistance at the same position on rice chromosome 11. Maeda H et al. (1999) used the Miryang 23 / Qiuguang recombinant inbred line population to detect a QTL for resistance to stripe leaf blight near XNpb257 between markers XNpb202 and C1172 on chromosome 11, with a contribution rate of 17.36%. The resistance gene effect comes from Miryang 23; Hayano-Saito et al. (1998) used the F obtained by crossing the resistant progeny Asanohikari of Modan with the susceptible japonica rice Koshihikari 2 The population will be derived from the stripe leaf blight resistance gene Stv-b of indica rice Modan i Located between the XNpb220 and XNpb257 markers on chromosome 11. Our research group has used the Kinmaze (japonica) / DV85 (indica) recombinant inbred line population to detect qStv11, which comes fr...

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Abstract

The present invention belongs to the molecular genetics field and relates to the the molecular marker method of the major gene loci conferring rice stripe disease resistance. The rice variety Nipponbare is hybridized with Kasalath and then backcrossed with Nipponbare. Via genetic linkage analysis of the genotype and disease index ratio of each family in aquired backcross inbred lines, the molecular marker BJ11-8 of the major rice stripe disease resistance gene loci qSTVll is obtained. Detection of this major gene locus in Kasalath and its derivatives via the molecular marker BJ11-8 will forecast the resistance level to the rice stripe disease and increase the selection efficiency of rice stripe disease resistant rices significantly.

Description

1. Technical field [0001] The invention provides a molecular marker method that can be used for breeding of the rice stripe blight resistance main gene locus qSTV11, belongs to the field of molecular genetics, and is specially used for the breeding of rice stripe blight-resistant varieties and the utilization of germplasm resources . 2. Technical background [0002] Rice stripe leaf blight is a viral disease caused by rice stripe virus, and its vector is mainly the small planthopper. In recent years, with the popularization and application of light cultivation techniques such as no-tillage, rice-wheat interleaving, and wheat-introducing rice, as well as susceptible high-yielding varieties, the expansion and harm of the SBPH carrier population of rice stripe leaf blight has been accelerated. The characteristics of short sucking time and long-lasting virus transmission of SBPH make pest control and disease prevention very difficult, and the occurrence of rice stripe leaf blig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 万建民孙黛珍江玲张迎信陈亮明刘世家刘喜王春明程遐年翟虎渠
Owner NANJING AGRICULTURAL UNIVERSITY
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