255results about How to "Not affected by the environment" patented technology

The invention relates to an intelligent fountain control method and system. The system comprises electronic tags, a control module, a frequency converter, a fountain executing mechanism and a reader-writer. The reader-writer reads and transmits identity information to the control module; the control module is used for receiving and caching the identity information, performing identity information authentication and transmitting control information to the frequency converter after the identity information is authenticated; the frequency converter controls on and off of the fountain executing mechanism. The intelligent fountain control system has the advantages that by means of the identity information set for every electronic tag, intelligent fountain control can be achieved according to different identity information to achieve the effects of different fountain shows,, so that water saving, electricity saving and service life prolonging can be obtained; by identifying whether adult identity information exists through the reader-writer to determine whether children are safe to surround a fountain, the safety of the fountain can be improved, so that the management cost can be reduced, and the problem of aesthetic fatigue due to frequent watching of the same fountain show can be solved.

The invention discloses a walking type cleaning device for high-rise glass curtain walls. The walking type cleaning device comprises a suspension device and a cleaning device; the suspension device isfixedly connected with the cleaning device through a traction rope; the cleaning device comprises a shell; a fixed suction cup frame and a movable suction cup frame communicate with a first negativepressure pump and a second negative pressure pump through pipelines respectively; a worm wheel is connected with a worm rod in a matched mode; a movable block is movably mounted on a threaded rod; a cleaning plate is sequentially provided with a water scraping strip, a plurality of cleaning discs and a plurality of cleaning spray heads from top to bottom; and a cleaning motor, a moving motor, thefirst negative pressure pump and the second negative pressure pump are electrically connected with a PLC circuit board. A machine is used for replacing manpower to perform high-rise operation; the twonegative pressure pumps are used for firmly adsorbing the cleaning device to the curtain wall; the movable suction cup frame is controlled to move for driving the device to walk; the glass curtain wall is cleaned by cleaning liquid and the cleaning discs; and the walking type cleaning device is high in cleanness, flexible to move and higher in working efficiency.

The invention discloses an ISSR molecular marking based method for constructing a Chinese asparagus bean generic resource database and application thereof. The invention also utilizes the data base to identify the trueness of a variety; according to field forms, the consistency and typicality among individual plants of the variety are identified, separated and atypical materials are given up, and DNA of the individual plant of the consistent and typical variety is picked up; by utilizing three ISSR markings with high polymorphism, the consistency of DNA level among the individual plants is primarily selected, and the variety and individual plant constructing a fingerprint are finally determined; and by utilizing the finally selected variety and individual plant, the ISSR analysis of the Chinese asparagus bean is made to construct a fingerprint database. The invention overcomes the defects existing in the construction of the prior generic resource database, such as separation among different individual plants of the variety and impurity on the DNA level, the reliability and accuracy of the Chinese asparagus bean ISSR fingerprint data base constructed by the method are greatly improved, and the planting varieties of the Chinese asparagus bean can be effectively and conveniently identified and distinguished.

The invention discloses a method for constructing a fingerprint of phaseolus vulgaris according to an SSR (simple sequence repeat) molecular marker and applications, and belongs to the technical field of finger print construction. The method comprises the following steps of extracting phaseolus vulgaris DNA (deoxyribonucleic acid), performing PCR (polymerase chain reaction) amplified reaction by utilizing the phaseolus vulgaris DNA and SSR core primers, and after electrophoresis detection, constructing the characteristic fingerprint of the phaseolus vulgaris according to an electrophoresis result; the nucleotide sequences of the SSR core primers are as shown by SEQIDNO.1-16. Compared with conventional morphological identification, the method disclosed by the invention is accurate and reliable in identification result and short in consumed time, is not influenced by environments, is simple in operation, can be used for identifying multiple varieties simultaneously, and is convenient and efficient.

The invention discloses an intensified artificial breeding method for Australian crayfishes. The method comprises the following steps of: putting pubescent parent crayfishes which pull through a wintering period into a spawning pond according to a proportion of 1-3: 1 of female and male, wherein circulating water is adopted in the spawning pond to automatically control and increase oxygen; sterilizing fertilized ovigerous female crayfishes, putting into small hatching lattices in a hatching pond, wherein one crayfish is hatched in each small hatching lattice, and carrying out water-flow hatching; and enabling hatched young crayfish seeds to leave away from mother bodies after 30-50 days of incubation period, enabling the young crayfish seeds to escape into water through a filter screen of the hatching pond, after all the crayfish seeds at the abdomen of the ovigerous female crayfishes leave away from the mother bodies, immediately separating the parent crayfishes, and remaining the young crayfish seeds to be continuously cultivated for 5-10 days, so that the cultivation can be carried out. According to the method, the artificial intensified breeding incubation can be carried out according to the required offspring seed quantity and the required offspring seed time at any time, the water temperature, the dissolved oxygen, the water quality and the like can be controlled, the method can not be influenced by environment, and the method is high in fertilizing rate, spawning rate and hatching rate.

The invention relates to a molecular marker for mungbean weevil resistance gene linkage, which is used for screening mungbean weevil resistant genes. V2709, VC1973 (zhonglu No. 1) and the individual DNA of F2 separation population thereof are extracted by the CTAB method. 63 RAPD molecular markers, 100 pairs of SSR molecular markers and 28 pairs of STS molecular markers are adopted to carry out the PCR product polymorphism screening on the resistant bulk and the susceptible bulk of the two parents and the F2 progeny, and then each of F2 generation individuals is screened out the weevil resistance gene linkage marker. A RAPD primer, OPC-06 and a STS primer are found to link with a weevil resistance genes The molecular marker can target the weevil resistance gene, and the selection efficiency of weevil resistance breeding is greatly improved.

The invention provides a molecular marker Marker Sex 1 and an identification method for quickly identifying genetic sex of portunus trituberculatus, wherein the Marker Sex 1 has the nucleotide sequence represented as the SEQ ID No.1. The identification method includes steps of: extracting genome DNA from muscle tissue of the portunus trituberculatus; with the genome DNA as a template, performing aPCR amplification reaction; purifying and sequencing the PCR amplification product; comparing the sequencing result with the DNA sequence of the molecular marker, and determining male if double peakexists or female if single peak exists. The identification method is simple and quick in operation and is low in demand on the sample, has high identification accuracy, is free of influence due to tissue specificity and environment, and has important effect for establishing all-female or high-female-ratio larval breeding technologies.

The invention relates to the technical field of molecular markers, and specifically relates to SNP molecular markers related to stearic acid content in camellia oleifera seed oil and applications of the SNP molecular markers. Two SNP molecular markers PB.35271.1-548 and PB.35271.1-555 related to the stearic acid content in camellia oleifera seed oil are provided; the SNP molecular marker PB.35271.1-548 contains the nucleotide sequence shown as SEQ ID NO.1 and of which polymorphism is C/T at the position 548th site; and the SNP molecular marker PB.35271.1-555 contains the nucleotide sequence shown as the SEQ ID NO.1 and of which polymorphism is G/A at the position 555th site. Through the utilization of the two markers to identify the stearic acid content phenotype of camellia oleifera, theidentification and auxiliary screening of seedling stages can be realized, the selection efficiency of camellia oleifera breeding can be effectively enhanced, and breeding process can be accelerated.

The invention relates to a method for monitoring high-precision deformation in real time in tunneling construction, and a calculating algorithm. The method comprises the following implementation steps: A, installing radio frequency pseudo-satellite positioning base stations at an excavated tunnel face and in a tunnel portal space region, and precisely determining base station coordinates; B, installing a wireless positioning data receiving terminal for representing a location identity thereof on each to-be-monitored point, and receiving a RF radio signal emitted by each base station; C, receiving observation data between each terminal and each base station in real time through a software system, calculating high-precision displacement information of the terminal and broadcasting displacement monitoring information to an engineering informatization management platform; D, visualizing real-time displacement deformation and accumulated displacement deformation of each monitoring point; and E, performing risk early-warning by setting a threshold through an early-warning system of the monitoring point displacement deformation, wherein the system can immediately triggers an automatic warning system once the displacement deformation exceeds the threshold, and an operator on duty can immediately adopt a corresponding safety emergency measure to guarantee construction security.

The invention discloses a method for prefabricating and constructing a separated rail structure. The separated rail structure comprises a separated rail bearing groove and a factory-prefabricated rail system. The method for prefabricating the separated rail structure includes the following steps of preparing materials, controlling temperatures, arranging the rail bearing groove, cleaning a groove area, mounting a tie plate under a rail, placing the steel rail, checking and sealing the end, treating before pouring and pouring polymer materials. The method for constructing the separated rail structure includes the following steps of hoisting a rail bearing groove rail system, conducting foundation treatment, placing the prefabricated rail bearing groove and adjusting the geometry, connecting and fixing, pouring concrete, maintaining and removing tools. According to the method for prefabricating and constructing the separated rail structure, the construction technologies of factory prefabrication, field assembly and the like are used, the fastening-down rail temperature of the rail system is effectively controlled in a factory, the influence of the site construction environment and climate is avoided, the construction cost of the rail is lowered, the efficiency is improved, and maintaining and replacing are more convenient.

The invention relates to the field of molecular genetics, in particular to the application of a molecular marker method in pig breeding for disease resistance, wherein according to the molecular marker method, the molecule at a mutation site in a CYP3A88 gene 5' regulatory region of a pig is marked. The inventor of the method discovers that the CYP3A885' regulatory region of a large Chinese streaky-head pig and the CYP3A885' regulatory region of a Duroc long hybrid pig have a plurality of differences, wherein an A-to-T mutation exists at the -78 site, through a luciferase reporter gene system, the fact that promoter activity of the -78 site is lowered remarkably after an A at the -78 site is mutated into a T in a site-directed mutagenesis mode is found, and the promoter activity of the -78 site is the same as the low level of messenger ribonucleic acid (mRNA) expression of a CYP3A88 gene of the porcine reproductive and respiratory syndrome (PRRS)-resistant large Chinese streaky-head pig. Therefore, through genotype detection of the -78 site of the CYP3A88 regulatory region in a pig genome, the genotype of the -78 site of the CYP3A88 regulatory region can be used as a modular marker associated with traits of the PPRS, the molecular marker method not only is simple, convenient and rapid, but also cannot be affected by the environment, and early selection for breeding can be realized.

The invention relates to the field of pavement condition monitoring, in particular to a mobile monitoring system for expressway pavement condition. The mobile monitoring system comprises a data acquisition module, a data input module, a data processing module, a data display module, a control module and an execution module. The data acquisition module used for acquiring system data is connected with the data input module, and the data input module used for inputting acquired data is connected with the data processing module, wherein the data processing module is used for processing and generating system data. The data processing module used for processing all input information data is connected with the data display module and the control module, wherein the data display module is used fordisplaying processed data, and the control module is used for controlling equipment. The control module for controlling all the equipment is connected with the execution module, wherein the executionmodule is used for implementing the control module. The mobile monitoring system of the expressway pavement condition can control the lighting time of traffic lights according to traffic flow and human flow on transverse and longitudinal roads.

The invention relates to the technical field of molecular markers, in particular to an SNP molecular marker related to palmitoleic acid content in camellia oleifera seed grease and application thereof. The invention provides two SNP molecular markers PB.10070.2-202 and PB.7527.1-77 related to palmitoleic acid content in camellia oleifera seed grease. The PB.10070.2-202 contains a nucleotide sequence with polymorphism of G/A at the 202nd site of the sequence shown as SEQ ID NO.1; the PB.7527.1-77 contains a nucleotide sequence with polymorphism of G/A at the 77th site of the sequence shown as SEQ ID NO.2. The two markers are used for identifying the palmitoleic acid content phenotype of camellia oleifera, seedling stage identification and auxiliary screening can be achieved, the selection efficiency of camellia oleifera breeding is effectively improved, and the breeding process is accelerated.

The invention belongs to the technical field of plant molecular biology and particularly relates to an SCAR molecular marker and an identification method for identifying banana fusarium wilt resistance. By means of the SCAR molecular marking technology, a special strip of banana fusarium wilt susceptible varieties is screened out, a specificity primer is designed according to the sequence of the strip for specificity amplification, and the primer is converted into the SCAR molecular marker. The SCAR molecular marker and the method are used for identifying banana fusarium wilt resistance of banana varieties, provide a molecular assisting technology for banana disease-resistant new variety breeding, and can be further used for auxiliary screening of new banana varieties (strains) with banana tissue culture seedlings, banana field variant strains, chemically induced progenies and the like as screening populations.

The invention relates to a cable recognition device and a recognition method. The device comprises a host, an output line, a recognition soft coil and a receiving box, wherein the host is used to display a power supply state and an output state and used to send out an emission signal; the output line is used to transmit the emission signal to a to-be-recognized cable; the recognition soft coil is used to receive a sensing signal of the to-be-recognized cable; and the receiving box is used to receive and display the sensing signal. The method comprises the steps that the host outputs the emission signal; the output line transmits the emission signal to the to-be-recognized cable; the recognize soft coil receives the sensing signal of the to-be-recognized cable; a receiving machine identifies the sensing signal, extracts a pulse sting, carries out phase detection of the pulse string, and displays a pulse waveform and a pulse direction obtained after the detection; and according to the pulse waveform and the method, whether the to-be-recognized cable is a target cable can be determined, or whether a fault exists in the to-be-recognized cable can be judged. In comparison with the prior art, the device and the method provided by the invention have the advantages that power supply is convenient; use is simple; judgment is visualized; one device can be used for multiple purposes; and the device can be used at complicated sites, etc.

The present invention relates to an impression roller which is used for a filament conveying device. The impression roller comprises a shaft and a shaft housing which is rotatably kept on the shaft. Hereon, the shaft housing is connected with a bearing outer ring of a rolling bearing through a mode without relative rotation. The rolling bearing is elastically supported on the shaft through an inner ring and an elastic gasket. According to the invention, for axial stability, the inner ring of the rolling bearing is provided with an annular supporting trough for accommodating the gasket, wherein the gasket is sandwiched between the supporting trough and the shaft.

The invention discloses microsatellite primers for identifying crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas and a hybrid thereof and an identification method.The microsatellite primers are F:5'-CGACTGGTGGGAGTTTCTGAC-3' and R:5'-GCCGCTTCTATCTCCTTTGC-3'.Due to the fact that the external morphology of oysters changes greatly along with different living environments, identification is usually difficult only according to morphological characteristics; the oysters in the larval stage are more difficult to distinguish according to the morphological characteristics.By means of the microsatellite primers and the identification method, crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas individuals can be identified rapidly and accurately, and identification of the hybrid individual of the crassostrea hongkongensis, crassostrea ariakensis and crassostrea gigas individuals has the potential application value.Meanwhile, the microsatellite primers for identifying the crassostrea hongkongensis, the crassostrea ariakensis and the crassostrea gigas and the hybrid thereof and the identification method have the advantages of being simple in method, visual, accurate and effective in result, free of influences of environment and growth stage, low in cost and the like.

The invention relates to a flexible power generation device and a power generation backpack applying the same. The flexible power generation device comprises a back plate and a sliding plate capable of sliding relatively. A resilient mechanism for driving the sliding plate to slide upward is arranged between the back plate and the sliding plate. A generator is fixed to the sliding plate. A rotating shaft is rotatably connected to the sliding plate. The rotating shaft and the generator rotate homodromously through a single-direction transmission mechanism. A rope winds the rotating shaft, ropefasteners are fixed to the upper and lower ends of the back plate. The upper and lower ends of the rope are fixed to the rope fasteners. When the sliding plate slides downward, the rotating shaft is pulled by the rope to rotate, the rotating shaft drives the generator to rotate in a single direction to generate electricity through the single-direction transmission mechanism, and the resilient mechanism is compressed to have an elastic force so as to drive the sliding plate to reset. The power generation device is not affected by an environment, and the resilient mechanism can collect mechanical energy generated by the backpack which vibrates up and down when a human body walks or runs, and the mechanical energy is further converted into electric energy.

The invention discloses a greenhouse heating system and a control method thereof. The greenhouse heating system comprises a thermal-insulation water tank and a greenhouse, wherein one end of the thermal-insulation water tank communicates with a solar circulating pump and a solar heating device through a solar heating water inlet pipeline; the other end of the thermal-insulation water tank communicates with the solar heating device through a solar heating water return pipeline; the thermal-insulation water tank communicates with an air heat pump through a pipeline; a radiator is arranged in the greenhouse; one end of the radiator communicates with a greenhouse heating circulating pump and the thermal-insulation water tank through a greenhouse heating water inlet pipeline; the other end of the radiator communicates with the thermal-insulation water tank through a greenhouse heating water return pipeline; and the solar circulating pump, the air heat pump, the radiator and the greenhouse heating circulating pump are electrically connected with a controller separately. The greenhouse heating system can be used for heating the greenhouse by converting solar energy into heat energy; various heating devices are monitored by a sensor; the controller controls the heating devices, so that the temperature in the greenhouse is kept within a high-precision temperature range, and therefore, the greenhouse heating system is more environmental-friendly, and cost is saved.