Molecular marker linked with bruchid resistance gene in mung bean

Abstract

The invention relates to a molecular marker for mungbean weevil resistance gene linkage, which is used for screening mungbean weevil resistant genes. V2709, VC1973 (zhonglu No. 1) and the individual DNA of F2 separation population thereof are extracted by the CTAB method. 63 RAPD molecular markers, 100 pairs of SSR molecular markers and 28 pairs of STS molecular markers are adopted to carry out the PCR product polymorphism screening on the resistant bulk and the susceptible bulk of the two parents and the F2 progeny, and then each of F2 generation individuals is screened out the weevil resistance gene linkage marker. A RAPD primer, OPC-06 and a STS primer are found to link with a weevil resistance genes The molecular marker can target the weevil resistance gene, and the selection efficiency of weevil resistance breeding is greatly improved.

Description

1. Technical field [0001] The invention discloses a molecular marker linked with a mung bean anti-weevil gene, which is used for molecular breeding to improve mung bean resistance to the weevil, and is specially used for screening the mung bean anti-weevil gene. 2. Technical background [0002] Mung beans are rich in protein and are a source of food for the survival of many countries [Maurício Pereira Sales, Isabel Rodrigues Gerhardt, Maria Fátima Grossi-de-Sá, et al. Do legume storage proteins play a role indefending seeds against bruchids? Plant Physiology, 2000, 124: 515-522], bean weevil is an important storage pest of edible bean crops, and it can occur in any place where edible beans are grown and stored. The bean weevils that damage mung beans mainly include mung bean weevil (Callosobruchus chinensis L.), four-striped bean weevil (Callosobruchus maculatus F.) and chickpea weevil (Callosobruchus analis E.), among which the mung bean weevil is the most serious damage. ...

AI Technical Summary

Problems solved by technology

Third, mung bean is a small miscellaneous grain crop. Compared with large crops, basic research is very weak. Today, when large crops are vigorously developing molecular marker-assisted breeding, mung bean still faces many problems such as lack of primers and few known basic sequences. New varieties have not yet appeared [Prakit S, ChanidaA, PeterAC. Inheritance of seed resistance to bruchids in cultivated mungbean (Vigna radiate, L.Wilczek). Euphytica, 2007, 155: 47~55]; There are few insect materials and whether the resistance to pests and diseases is long-lasting