Molecular marker linked with bruchid resistance gene in mung bean

A technology of molecular markers and gene linkage, applied in the field of mung bean anti-weevil gene screening, can solve the problems of weakness, few insect-resistant materials, disease and insect resistance, and few known basic sequences, so as to save time and cost, and select the target clearly Effect

Inactive Publication Date: 2009-02-04
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Third, mung bean is a small miscellaneous grain crop. Compared with large crops, basic research is very weak. Today, when large crops are vigorously developing molecular marker-assisted breeding, mung bean still faces many problems such as lack of primers and few known basic sequences.

Method used

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Embodiment Construction

[0017] The implementation procedure of the present invention is: in 2004, V2709 was hybridized with VC1973A (Zhonglv No. 1), F 1 F 2 Seeds were planted in Chaoyang Louzizhuang Experimental Station in April 2006, young leaves were collected, seeds were harvested from individual plants after maturity in July, and artificial inoculation identification was carried out in order to infer F 2 genotype.

[0018] As a result of infestation identification, it was found that 56 individual plants were completely susceptible to insects, 39 individual plants were completely resistant to insects, and 92 individual plants were partially susceptible and partially resistant to insects. The construction of insect-resistant and insect-susceptible pools is mainly based on the 3 F 2 Genotype, select typical homozygous resistant and homozygous susceptible F 2 Individuals form insect-resistant and insect-susceptible pools.

[0019] Using CTAB method to extract V2709, VC1973A (Zhonglv 1) and its ...

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Abstract

The invention relates to a molecular marker for mungbean weevil resistance gene linkage, which is used for screening mungbean weevil resistant genes. V2709, VC1973 (zhonglu No. 1) and the individual DNA of F2 separation population thereof are extracted by the CTAB method. 63 RAPD molecular markers, 100 pairs of SSR molecular markers and 28 pairs of STS molecular markers are adopted to carry out the PCR product polymorphism screening on the resistant bulk and the susceptible bulk of the two parents and the F2 progeny, and then each of F2 generation individuals is screened out the weevil resistance gene linkage marker. A RAPD primer, OPC-06 and a STS primer are found to link with a weevil resistance genes The molecular marker can target the weevil resistance gene, and the selection efficiency of weevil resistance breeding is greatly improved.

Description

1. Technical field [0001] The invention discloses a molecular marker linked with a mung bean anti-weevil gene, which is used for molecular breeding to improve mung bean resistance to the weevil, and is specially used for screening the mung bean anti-weevil gene. 2. Technical background [0002] Mung beans are rich in protein and are a source of food for the survival of many countries [Maurício Pereira Sales, Isabel Rodrigues Gerhardt, Maria Fátima Grossi-de-Sá, et al. Do legume storage proteins play a role indefending seeds against bruchids? Plant Physiology, 2000, 124: 515-522], bean weevil is an important storage pest of edible bean crops, and it can occur in any place where edible beans are grown and stored. The bean weevils that damage mung beans mainly include mung bean weevil (Callosobruchus chinensis L.), four-striped bean weevil (Callosobruchus maculatus F.) and chickpea weevil (Callosobruchus analis E.), among which the mung bean weevil is the most serious damage. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H1/04
Inventor 程须珍孙蕾王素华王丽侠刘长友梅丽徐宁
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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