Molecular marker method of rice panicle blast resistance gene

Abstract

The invention belongs to the field of crop molecular genetics and breeding, and discloses a molecular marker method of a rice panicle blast resistance gene. The method comprises the following steps: (1) preparing a rice sample, and extracting genome DNA of the rice sample; and (2) conducting PCR amplification on the genome DNA of the rice sample by virtue of primers of any one pair of molecular markers of RM27187 and RM27381, and conducting electrophoresis detection on a PCR amplification product, wherein it is indicated that the Pb-hk1 gene exists when a molecular marker DNA segment of corresponding size is amplified. With the application of the molecular marker of the rice panicle blast resistance gene Pb-hk1 provided by the invention, it can detect that whether back-shell rice and hybrid, backcross and repeated backcross generations contain the gene or not and can predicate a level of resisting to rice blast, so that a rice panicle blast generating material selecting efficiency is greatly improved and a progress of disease-resistance breeding is accelerated.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and relates to a molecular marker method for a rice panicle blast resistance gene. Background technique [0002] Rice is one of the most important food crops in our country, which is of great significance to ensure our country's food security and national economic growth. Rice blast is the most serious disease in rice production in my country, and has the characteristics of rapid spread, wide occurrence, and severe damage (Ling Zhongzhuan et al., 1989, Research on the Physiological Race of Magnaporthe grisea in Northern my country. Agricultural Sciences of China, 22(3 ):7-13). It is the most economical and effective way to control the occurrence of rice blast and reduce the loss of rice yield by further exploring and utilizing the genetic resources of rice blast resistance in my country and cultivating and planting disease-resistant varieties. [0003] So far, scientists from ...

AI Technical Summary

Problems solved by technology

However, traditional breeding methods are time-consuming, laborious, phenotypic identification is difficult, and breeding efficiency is low. Since most disease resistance genes are dominant and epistasis interactions often exist between genes, the aggregation of disease resistance genes is even more difficult.

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