Molecular marker method of rice panicle blast resistance gene

A technology of molecular markers and blast resistance, which is applied in the field of crop molecular genetics and breeding, can solve the problems of difficult aggregation of disease-resistant genes, difficult phenotype identification, and low breeding efficiency, and achieves convenient identification, low reliability, and high cost. Effect

Active Publication Date: 2017-05-10
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, traditional breeding methods are time-consuming, laborious, phenotypic identification is difficult, and breeding efficiency is low. Since most disease resistance genes are dominant and epistasis interactions often exist between genes, the aggregation of disease resistance genes is even more difficult.

Method used

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  • Molecular marker method of rice panicle blast resistance gene
  • Molecular marker method of rice panicle blast resistance gene
  • Molecular marker method of rice panicle blast resistance gene

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Embodiment 1

[0027] (1) Materials and methods:

[0028] 1. Li Peifu et al. obtained F 1 , selfing to obtain F 2 The isolated population was used for genetic analysis, and it was clarified that the resistance of Heijiazi japonica to the "Bei 1" strain was controlled by the interaction of two pairs of suppressor genes. On this basis, the present invention adopts single-grain transmission method to construct F 2:8 Recombinant inbred line population, that is, two parents are crossed to obtain F 1 , selfing to obtain F 2 , F 2 A single plant was selfed to produce lines, which were continuously selfed for 6 generations, and finally constructed into a F with 162 lines 2:8 Recombinant inbred populations. Use this population to locate a major locus related to panicle blast resistance, which is located between the markers RM27187 and RM27381 on the 11th chromosome, and is closely linked with these two markers, with a genetic distance of 6.34cM. Less than 34.00% resistance.

[0029] 2. For th...

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Abstract

The invention belongs to the field of crop molecular genetics and breeding, and discloses a molecular marker method of a rice panicle blast resistance gene. The method comprises the following steps: (1) preparing a rice sample, and extracting genome DNA of the rice sample; and (2) conducting PCR amplification on the genome DNA of the rice sample by virtue of primers of any one pair of molecular markers of RM27187 and RM27381, and conducting electrophoresis detection on a PCR amplification product, wherein it is indicated that the Pb-hk1 gene exists when a molecular marker DNA segment of corresponding size is amplified. With the application of the molecular marker of the rice panicle blast resistance gene Pb-hk1 provided by the invention, it can detect that whether back-shell rice and hybrid, backcross and repeated backcross generations contain the gene or not and can predicate a level of resisting to rice blast, so that a rice panicle blast generating material selecting efficiency is greatly improved and a progress of disease-resistance breeding is accelerated.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and relates to a molecular marker method for a rice panicle blast resistance gene. Background technique [0002] Rice is one of the most important food crops in our country, which is of great significance to ensure our country's food security and national economic growth. Rice blast is the most serious disease in rice production in my country, and has the characteristics of rapid spread, wide occurrence, and severe damage (Ling Zhongzhuan et al., 1989, Research on the Physiological Race of Magnaporthe grisea in Northern my country. Agricultural Sciences of China, 22(3 ):7-13). It is the most economical and effective way to control the occurrence of rice blast and reduce the loss of rice yield by further exploring and utilizing the genetic resources of rice blast resistance in my country and cultivating and planting disease-resistant varieties. [0003] So far, scientists from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/124C12Q2600/156
Inventor 鲍永美张红生方能炎王建飞黄骥王州飞程金平
Owner NANJING AGRICULTURAL UNIVERSITY
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