A ssr marker related to cucumber fruit length traits and its application

A fruit and cucumber technology, applied in the fields of genetic engineering and molecular biology, can solve the problems of different numbers and positions of QTL, lack of anchor markers, and failure to achieve fine positioning of fruit length-related genes, so as to solve the problems of low reliability and identification Convenience and accurate genetic loci

Active Publication Date: 2021-04-30
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Previous studies have been conducted on cucumber fruit length (Kennard&Havey, 1995; Serquen et al, 1997; Fazio et al, 2003; Yuanet al, 2007), and the melon length QTLs detected in these reports are between 4 and 6, However, due to the different test materials used, the number and location of QTL are also different, and the same anchor markers are lacking, and the fine mapping of fruit length-related genes has not been realized.

Method used

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  • A ssr marker related to cucumber fruit length traits and its application
  • A ssr marker related to cucumber fruit length traits and its application

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Experimental program
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Embodiment Construction

[0027] The major QTL loci and linked molecular markers for cucumber fruit length were obtained by the following methods:

[0028] 148 plants of F 2 Progeny single plant.

[0029] Using the SLAF-seq method to analyze the parents and 148 strains of F 2 High-throughput simplified genome sequencing was carried out in the next generation, and a total of 7 linkage groups of cucumber were obtained from the analysis. The sequencing generated 10.76Gb of raw data, and the average sequencing depth of the parents was 53.48×, F 2 The average sequencing depth of the generation was 7.74×, and a total of 1800 SNP markers were developed, and the first high-density SNP genetic map of cucumber was constructed.

[0030] F 2 F 2:3 Families, planted F in spring 2013 and autumn 2014 2:3 Family, used for statistics of fruit length traits. Plant 10 plants in each family number, select a commercial melon and mature melon for each plant, and measure the length of its fruit. The measurement standar...

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Abstract

The invention provides SSR markers related to cucumber fruit length traits and applications thereof, belonging to the field of plant molecular breeding. Molecular marker SSR05572 closely linked to cucumber fruit length gene was disclosed for the first time, and the forward and reverse primer sequences of the marker were: 5'GCAAACCATAAGTTTCCCCA 3' and 5'GATCGATATTGCAACGAATTACA3', respectively. In BC4‑S2 constructed from the long-melon variety cucumber and the short-melon variety cucumber, the primers SSR05572F / SSR05572R could amplify a 204bp product in the long-melon strain and a 191bp product in the short-melon strain. The product containing two bands of 204bp and 191bp can be amplified in the heterozygous line, which is closely linked with the fruit length gene. The molecular marker closely linked with the cucumber fruit length gene disclosed by the invention can be used for cucumber fruit length gene positioning or molecular marker assisted breeding. It has important theoretical and practical guiding significance for accelerating the genetic improvement process of cucumber varieties and improving the efficiency of breeding selection.

Description

technical field [0001] The invention relates to genetic engineering and molecular biology, belongs to the field of molecular genetic breeding, provides SSR markers related to cucumber fruit length and application thereof, which can be used in molecular marker-assisted selective breeding of cucumber fruit length traits to improve breeding efficiency. [0002] technical background [0003] Cucumber (Cucumis sativus L.2n=2x=14) belongs to Cucurbitaceae Melon, an annual vine herb, and is one of the top ten vegetable crops in the world. my country's cucumber planting area and total output rank first in the world, but my country's cucumber output per unit area and export volume are lower than many countries. One of the key reasons for this contrast is the variety of high-quality cucumber varieties, especially Cucumber varieties suitable for protected cultivation are relatively scarce. Molecular-assisted selection can shorten the breeding cycle and improve breeding efficiency, and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 娄群峰曹蕾杨康魏庆镇
Owner NANJING AGRICULTURAL UNIVERSITY
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