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A kind of SNP molecular marker related to the trait of multiple trichomes in cucumber and its application

A technology of molecular markers and epidermis, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA / RNA fragments, etc., can solve the problems of molecular marker-assisted breeding difficulties, etc., to achieve convenient identification, solve low reliability, and gene loci point precise effect

Active Publication Date: 2022-02-18
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most cucumber trichome-deficient mutants have a hairless phenotype, but the research on cucumber hirsutism is still blank, which brings difficulties to the genetic improvement and molecular marker-assisted breeding of cucumber fruit skin-related traits

Method used

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  • A kind of SNP molecular marker related to the trait of multiple trichomes in cucumber and its application
  • A kind of SNP molecular marker related to the trait of multiple trichomes in cucumber and its application
  • A kind of SNP molecular marker related to the trait of multiple trichomes in cucumber and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Cucumber multiple epidermis trait linkage molecular markers are obtained by the following methods:

[0033] (1) Acquisition and genetic analysis of the trait of multiple epidermis in cucumber

[0034] Cucumber multiple trichomes mutant mt was obtained by mutagenizing the northern cucumber cultivar 'Changchun Mici' with EMS (ethyl methanesulfonate). Compared with wild-type plants, the mt mutant had more epidermal trichomes on the surface of leaves, stems, tendrils, floral organs and ovaries. F1 plants of WT and mt crosses exhibited an intermediate phenotype with a moderate number of epidermal trichomes on the surface. The length of the epidermal trichomes of the mt mutant was shorter than that of the wild type, and the length of the epidermal trichomes of the F1 was intermediate between the parents. Microscopic observation revealed that the number, size, arrangement and shape of epidermal trichomes in leaves of mt were different from those of wild type. In a...

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Abstract

The invention discloses a SNP molecular marker related to the trait of multi-skinned cucumber and its application. The SNP molecular marker is based on the DNA sequence of 400 bp before and after the Chr6:27397366 site in the 'Chinese Long v2' cucumber genome database. Primers were designed on the template, and the amplified genome sequence was shown as SEQ ID NO: 1. From the 5' end, there was an A / C polymorphism at the 401st position of the sequence, that is, the SNP site. The molecular marker of the invention is closely linked with the cucumber multi-trichosis gene, is reliable and quick to detect, and can be used for directional genetic breeding of cucumber multi-trichosis related traits. It has important theoretical and practical guiding significance for accelerating the genetic improvement process of cucumber varieties and improving the efficiency of breeding selection.

Description

technical field [0001] The invention relates to a SNP molecular marker related to the multi-skinned trichomes trait of cucumber and an application thereof, belonging to the field of molecular genetic breeding of cucumber. Background technique [0002] Epidermal hairs (Trichomes) develop from epidermal cells. It is divided into unicellular or multicellular, some branched and some not branched. Some of the epidermis have glands, which can secrete some lipid substances or secondary metabolites, and some have no glands. Different types of epidermis can be produced on the same plant. Plant epidermal trichomes have a series of functions: non-glandular epidermal trichomes can dissipate heat, increase tolerance to chilling damage, facilitate seed dispersal, absorb water, protect from ultraviolet rays and damage plant tissues from some herbivorous insects; glandular trichomes Cuticle hairs help plants resist herbivorous insects and pathogens by secreting chemicals, but they also ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2600/172
Inventor 娄群峰杨志歌宋蒙飞陈劲枫
Owner NANJING AGRICULTURAL UNIVERSITY
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