51 results about "Hairless" patented technology

This invention relates to compounds, compositions, and methods useful for modulating hairless (HR) gene expression using short interfering nucleic acid (siNA) molecules. This invention also relates to compounds, compositions, and methods useful for modulating the expression and activity of other genes involved in pathways of hairless gene expression and/or activity by RNA interference (RNAi) using small nucleic acid molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules and methods used to modulate the expression of hairless (HR) genes.

This invention discloses a process for synthesizing a hairy polymer particle which comprises the steps of (1) polymerizing a vinyl aromatic monomer by emulsion polymerization in an aqueous medium to produce core particles, (2) recovering the core particles from the aqueous medium, (3) dispersing the core particles in an organic solvent, (4) adding an organo-lithium compound to the dried core particles in the organic solvent to produce the hairless core initiator, and (5) utilizing the hairless core initiator to initiate the anionic polymerization of a conjugated diolefin monomer in an organic solvent to produce a solution of the hairy polymer particles. The hairy polymer nanoparticles can then be recovered from the organic solvent. These hairy polymer particles are comprised of (1) a core which is comprised of a polymer of a vinyl aromatic monomer and (2) hairs which are polymer chains of a conjugated diolefin monomer, wherein the hairs are covalently bonded to the core. The core is typically spherical in shape, has a diameter of less than 1000 nm, and is comprised of a crosslinked polymer of a vinyl aromatic monomer. The hairy polymer particles of this invention are useful as fillers in rubber compositions used in making articles of manufacture, such as tires, hoses, power transmission belts, windshield wiper blades, and the like.

The invention provides a Chinese medicine composition preventing and curing varicella, which comprises the following components by weight parts: 4-9 parts of honeysuckle, 4-9 parts of forsythia, 4-9 parts of radix paeoniae alba, 4-9 parts of radix scutellariae, 4-9 parts of gentiana scabra bge, 4-9 parts of radix puerariae, 4-9 parts of platycodon root, 4-9 parts of herba schizonepetae, 4-9 partsof radix angelicae, 6-10 parts of radix isatidis, 6-10 parts of dandelion, 3-7 parts of cicada ecdysis, 3-7 parts of bamboo leaves, 3-7 parts of lithospermum and 3-7 parts of liquorice; and the preparation method is characterized in that all the Chinese medicine components are weighted accurately according to the propotions, crushed, and evenly mixed. The Chinese medicine composition preventing and curing varicella not only can effectively restrain the replication of varicella virus, but also can clear heat, promote eruption, prevent variola from generating in hairless part of the skin and promote the variola from dropping; and the preparation method is simple and is applicable for the requirements of scale industrial production.

The invention discloses a mononucleotide polymorphic marker site, primers and a kit for identifying peach fruit epidermal hair characters, and application thereof. The mononucleotide polymorphic marker site is 17576893th nucleotide of chromosome 5 of the peach genome, and the nucleotide is A or G. The 17576893th nucleotide mononucleotide polymorphic marker site of chromosome 5 of the peach genome can be used for identification or auxiliary identification on peach fruit epidermal hair characters, and has higher accuracy in the peach fruit epidermal hair character identification process. When the SNPs accuracy of 221 peach individuals to be detected in 15 hybrid populations is verified, the result indicates that the detection accuracy for peach fruit epidermal hairy character can reach 96% or above, the detection accuracy for peach fruit hairless character can reach 80% or above, and thus, the mononucleotide polymorphic marker site has higher accuracy. Thus, the mononucleotide marker site for detection is simple, has the advantages of high speed and low cost, and can implement large-scale application in production.

The invention relates to mutation site controllable hairless model pig reconstructed oocyte and a reconstruction method thereof and a model pig construction method. According to the hairless model pig reconstructed oocyte and reconstruction method thereof and the model pig construction method, by utilizing CRISPR/Cas9 and point mutation technology, cells can be accurately repaired by utilizing provided ssODNs as a template in a homologous recombination manner after Cas9 is cut at the gene specific site, and a termination codon is introduced at the gene specific site through the CRISPR/Cas9 and ssODNs design, so that gene translation is early terminated, and further the loss of gene function can be caused. Human disease gene mutation type can be accurately replicated by utilizing the method, so that the phenotype difference caused due to different gene mutation forms can be avoided. A hairless model pig constructed by utilizing the method has no other abnormities except for hair loss, hoof development defect and filiform papillae development defect, and can be used for various dermatological researches and external skin drugs and cosmetics. The skin transformed immunologically can be used for clinical skin transplantation.

Long term removal of hair is achieved by delivering a collagen cleaving agent intradermally or subdermally. This is thought to disrupt the fine collagen network anchoring the hair follicles within the dermis and additionally by altering the normal functioning of the dermal papilla cells located in the base of the hair follicle. The method of removal is applicable to animal husbandry methods such as maintaining the breech of sheep hairless and thus has application as a substitute to mulesing. The method is also applicable for cosmetic hair removal.

The invention belongs to the field of gene engineering, and particularly relates to a pair of allelic gene GL/gl for controlling haired/hairless property of cucumis melo. The gene gl for controlling the haireless property of cucumis melo is recessively homozygous in a hairless material, has a whole length of 536bp, and has a specific base sequence as shown in SEQ ID NO.1; the gene GL for controlling haired property of cucumis melo is dominantly homozygous or heterozygous in a haired material, and has a specific base sequence as shown in SEQ ID NO.2. The allelic gene GL/gl is used for finely mapping hairless property of cucumis melo for the first time, and realizes final cloning for GL/gl genes by further utilizing chromosome walking, and has an important meaning on understanding cucumis melo epidermal hair formation mechanism and controlling network thereof; and a stable specific dCAPS functional marker for the genes is developed according to the sequence difference of the genes in haired materials and hairless materials. The pair of allelic gene GL/gl can be simply and efficiently applied to molecular marker assisted breeding.

The invention discloses a method for preparing an isolated hair follicle in a process of treating scar by hair follicle stem cell transplantation. The method comprises the following steps: extractionof hair follicles, separation of the hair follicles, completely separating the hair follicle units into single hair follicles under an electron microscope, and keeping the extracted skin appendages such as sebaceous glands, sweat glands and the like as far as possible; for scars in a smooth hairless area, removing lower 1/3 of hair papilla, so that the hair follicle could not enter the next growthcycle, and no final hair grows in the scar area; in vitro culture of the hair follicles, putting the isolated single hair follicle into a special culture solution for culturing for later use; planting the hair follicle stem cells in a specific area of the scar on the basis of hair follicles. According to the method, through a hair follicle in-vitro culture technology, the single hair follicle isseparated and inactivated by adopting a separation method different from a hair transplantation operation, and is cultured in the special culture solution, so that growth and proliferation of hair follicle stem cells are facilitated. The method can achieve purposes of inhibiting scar hyperplasia, softening and repairing scars, and removing and beautifying scars.

The invention relates to a method for producing burn animal models with laser, which is characterized in that the laser is used to directly irradiate hairless or depilated animal skin, and the burns of I~IV° can be reproduced by adjusting the output power, irradiation time and irradiation distance. This model is easy to operate, the degree of burn can be precisely controlled, the success rate is high, the reproducibility is good, and the safety is high. It causes less pain to animals and conforms to the principles of animal welfare. Screening, drug efficacy evaluation, mechanism of action research, etc.

The present invention relates to topical compositions characterized in that they comprise as main constituent one or several fragments of hyaluronic acid with a molecular weight of 50,000 to 750,000 and, if desired, retinoids.

The invention relates to a method for detecting pig hair-free character based on EDAR gene expression quantity and application thereof, back skin tissue of a pig embryo is collected, the EDAR gene expression quantity is quantitatively detected through PCR, and the pig with the EDAR gene expression quantity lower than 3 is judged as a hair-free pig. The method for detecting the pig hair-free character based on the EDAR gene expression quantity can be used for assisting screening and breeding of hair-free pigs, and hair-free pig homozygotes can be obtained through breeding by knocking out EDAR. The candidate gene EDAR influencing the growth of the hair follicle substrate is screened out, an important reference and an animal model are provided for research of human hereditary alopecia diseases, meanwhile, a molecular marker with the hairless character and a large white pig hairless test group capable of being used for research are also provided, and an important pig hair-free strain animal model is provided for research on human hereditary alopecia, hair-free and other diseases.

The invention belongs to the technical field of animal specimen making and particularly relates to a method for making a stuffed animal specimen of hairless vertebrate and an injector adopted by the same. The invention discloses a special method for making a stuffed animal specimen of hairless vertebrate according to the basic characteristics of hairless vertebrate and based on the special advantages of alpha-cyanoacrylate compounds and by use of a specially designed alpha-cyanoacrylate compound injector. The front end of a needle is totally sealed; the inner wall of an inner cavity of the needle is completely and tightly inlaid with a teflon material, injection holes are uniformly distributed on the outer wall of the front end of the needle, and the alpha-cyanoacrylate compound is prevented from being adhered to the inner cavity wall of the needle and generating a polymerization reaction and blocking the injection channel; and moreover, residue is avoided, the number of needle holes of the injector in the animal specimen surface is greatly reduced, the problem of non-uniform filling is effectively solved, and the method for making a stuffed animal specimen of hairless vertebrate from alpha-cyanoacrylate compound is perfected.

The invention provides two dominant molecular marks cosegregation with tuberculate fruit (Tu) gene in cucumbers, wherein the first dominant molecular mark is composed of 236 nucleotides shown in the sequence table SEQ ID NO.1; and the second dominant molecular mark is composed of 468 nucleotides shown in the sequence table SEQ ID NO.2. Both the two dominant molecular marks disclosed by the invention exist in the cDNA areas of the tuberculate fruit (Tu) shown in the sequence table SEQ ID NO.7 and have high stability, and can be applied to the screening of cucumber types (except for the mutant variety g1 without thorns and tubercles) with/without tubercles around the world simply and rapidly and in high throughput. According to the cDNA areas of the tuberculate fruit (Tu) shown in the sequence table SEQ ID NO.7, a plurality of molecular marks can be designed as the dominant molecular marks for the screening of cucumber types (except for the mutant variety g1 without thorns and tubercles) with/without tubercles around the world, and the cDNA areas of the tuberculate fruit (Tu) can lay the molecular foundation for the clone of the glabrous gene and the gloss gene of cucumbers.

This invention relates to compounds, compositions, and methods useful for modulating hairless (HR) gene expression using short interfering nucleic acid (siNA) molecules. This invention also relates to compounds, compositions, and methods useful for modulating the expression and activity of other genes involved in pathways of hairless gene expression and/or activity by RNA interference (RNAi) using small nucleic acid molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules and methods used to modulate the expression of hairless (HR) genes.

The invention relates to a scabies ointment for livestock and a preparation method thereof. The scabies ointment for livestock is prepared from, by weight, 5-15 parts of flos lonicerae, 8-20 parts offructus forsythiae, 13-25 parts of rhizoma corydalis, 25-36 parts of plant soot, 3-7 parts of borneol, 15-25 parts of cacti, 15-26 parts of vegetable oil and 15-26 parts of lard. The scabies ointmentfor livestock can soften scabs and hard lumps on the skin surfaces of animals, remarkably relieve skin pruritus, simultaneously achieve the effects of relieving pain and diminishing inflammation, promote wound healing, soften the skin cutin and promote hair growth of hairless parts. After being smeared to wounds, caused by docking, castrate and the like, of animals, the scabies ointment can achieve the hemostatic and anti-inflammatory effects, promote wound healing and prevent occurrence of myiases. The ointment has the advantages that the healing rate of scabies is high, the cost is low, andno toxic or side effect occurs.

The present invention relates to fetal pig powder for male health-preserving and a manufacture method thereof. The fetal pig powder comprises male fetal pig powder and sorghum rice flour. The manufacture method comprises the following steps: firstly, newly born male fetal pigs are drowned by using vinegar or wine or warm water before the male fetal pigs drink pig milk or eat any food; then all hair of the drowned male fetal pigs are scraped completely; then the hairless male fetal pigs are crushed into granules; then the sorghum rice flour is added in the male fetal pig granules; and the two materials are mixed to obtain the fetal pig powder. The fetal pig powder prepared by mixing the male fetal pigs and materials is rich in nutrient ingredients; and the added sorghum rice flour can promote absorption of the fetal pig powder.

The present invention provides a hairless transgenic nonhuman animal used in the development of a therapy for dermatitis such as human atopic dermatitis and drug discovery. Specifically, the present invention provides a transgenic nonhuman animal, into which recombinant DNA comprising a heparin-binding EGF gene and a type 2 keratin gene promoter for regulating expression of the above gene has been introduced.