4 results about "Hairless" patented technology

The application discloses a gene BpMIXTA01 for regulating and controlling initial development of Broussonetia papyrifera epidermal hair as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene is shown in SEQ ID NO. 1, and the amino acid sequence is shown in SEQ ID NO. 2. The gene belongs to the ninth sub-group MIXTA gene of the R2R3 MYB transcription factor family. Function verification shows that overexpression of BpMIXTA01 in Arabidopsis thaliana can significantly increase the number of epidermal hairs, and can restore the epidermal hair loss phenotype of the Arabidopsis thaliana hairless mutant, proving that BpMIXTA01 is a positive regulation factor of epidermal hair formation. The application first discloses a key regulation gene for initial development of Broussonetia papyrifera epidermal hair cells, provides an important gene resource for analyzing the molecular mechanism of epidermal hair development of woody plants, and can cultivate a hairless forage Broussonetia papyrifera new variety through genetic engineering means, and has important theoretical value and application prospect.

This invention discloses a regulatory gene BpMYB090 for the development of epidermal hairs on paper mulberry leaves, its expressed protein, and its applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of this gene, BpMYB090, is shown in SEQ ID NO.1. This gene belongs to the ninth subgroup of the R2R3 MYB transcription factor family, MIXTA class, and its expression level is significantly positively correlated with the density of epidermal hairs on paper mulberry leaves. Overexpression of BpMYB090 in Arabidopsis thaliana significantly increases the number of epidermal hairs and restores the epidermal hair-deficient phenotype of the Arabidopsis thaliana mutant gl1, confirming that BpMYB090 is a positive regulator of epidermal hair formation. This invention reveals for the first time a key regulatory gene for the development of epidermal hairs on paper mulberry leaves. Furthermore, based on the positive regulatory function of this gene, new hairless forage paper mulberry varieties can be bred through gene silencing or editing techniques, possessing significant theoretical value and application prospects.

The invention relates to the technical field of animal genetic engineering, and particularly discloses a hairy severe combined immunodeficiency mouse model as well as a construction method and application thereof. The construction method of the hair-free severe combined immunodeficiency mouse model, disclosed by the invention, comprises the following steps: introducing a hair-free gene Foxn1 mutation into an NTG mouse, and constructing a homologous introduction inbred line. According to the technical scheme provided by the invention, multiple methods such as multi-gene breeding genotype identification, construction of a homologous introduction inbred line, a molecular marker-assisted selection method and IVF propagation are combined, so that a method for rapidly, accurately and directionally breeding the hairy severe combined immunodeficiency animals is established, and an experimental animal strain with genetic stability and phenotype stability is obtained.

This invention discloses a molecular marker and its application that breaks the linkage between smooth, hairless male sterility and co-segregation of watermelon fertility genes, aiming to solve the technical problem of the current lack of methods for identifying and characterizing major watermelon fertility genes. This application develops a molecular marker that breaks the linkage between smooth, hairless male sterility and co-segregation of watermelon fertility genes (based on the watermelon 97103 reference genome V2 version, which contains a genomic region related to male sterility in the 27.94-27.95 Mb region on chromosome 8; watermelons with deletions or partial deletions of this genomic region exhibit male sterility), and designs the corresponding primer pair BY2-3. Applying this molecular marker for marker-assisted selection breeding enables faster and more accurate targeted genetic improvement of watermelon fertility, thereby shortening the breeding cycle.