Method for preparing isolated hair follicle in process of treating scar by hair follicle stem cell transplantation
A technology of hair follicle stem cell transplantation, which is applied in the field of hair follicle treatment in vitro in the process of hair follicle stem cell transplantation to treat scars. It can solve the problems of long recovery time, unsatisfactory effect, and large side effects, and achieve softening of scars and strong in vitro proliferation ability. , Improve the effect of scar blood supply
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Embodiment 1
[0030] Step S1, hair follicle extraction, using FUE technology to extract a hair follicle unit;
[0031] Step S2, the separation of hair follicles, the hair follicle units are separated into complete single hair follicles under the electron microscope; the scar in the smooth hairless area is cut off the lower 1 / 3 of the dermal papilla, so that the hair follicles cannot enter the next growth cycle, and the scar area no terminal hair growth;
[0032] Step S3, the in vitro culture of hair follicles, the extracted and isolated single hair follicles are put into a special culture medium for cultivation, and the cultivation is carried out to enhance the activity of hair follicle stem cells and promote their differentiation and proliferation;
[0033] Step S4, hair follicle transplantation, relying on hair follicles to implant hair follicle stem cells into a specific area of the scar, to promote the proliferation and differentiation of hair follicle stem cells and the formation of ...
Embodiment 2
[0039] In this example, the effective rate of treating scars by hair follicle melanocyte stem cell transplantation is more than 100%. Through clinical comparison, the present invention finds that after the treatment of the isolated hair follicles during the treatment of scars by hair follicle stem cell transplantation, the scar repair time is significantly shortened. The curative effect is obviously improved.
[0040] The technical difficulty of the present invention: "the integrity of hair follicle stem cells and skin appendages" refers to whether the hair follicle stem cells can survive directly after planting, which is related to the later treatment effect. The autologous hair follicle stem cells that have been specially isolated and cultured in vitro have the characteristics of slow periodicity, strong self-renewal ability, high proliferation ability, and multi-differentiation potential, and can continue to repair scar tissue after transplantation. For scars in the smooth ...
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