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180 results about "Electron microscopic" patented technology

TEM MEMS device holder and method of fabrication

A device and method for fabricating a device holder for use with a standard holder body of a transmission electron microscope for use with in situ microscopy of both static and dynamic mechanisms. One or more electrical contact fingers is disposed between a baseplate and a frame, with a MEMS device making contact with the electrical contact fingers. A connector is provided to matingly engage the transmission electron microscope and the device holder to couple the device holder to the transmission electron microscope. Once clamped between the baseplate and frame, the electrical contact fingers may be separated from the template.
Owner:THE BOARD OF TRUSTEES OF THE UNIV OF ILLINOIS +2

Biofunctional magnetic nanoparticles for pathogen detection

This invention provides a method of detecting pathogens comprising the steps of: (a) contacting a sufficient amount of biofunctional magnetic nanoparticles with an appropriate sample for an appropriate period of time to permit the formation of complexes between the pathogens in the sample and the nanoparticles; (b) using a magnetic field to aggregate said complexes; and (c) detecting said complexes. The method may further comprise the additional step of removing said complexes. The biofunctional magnetic nanoparticles are preferably a conjugate of vancomycin and FePt. The pathogens may be bacteria or viruses, and the sample may be a solid, liquid, or gas. Detection may involve conventional fluorescence assay, enzyme-linked immunosorbent assay (ELISA), optical microscope, electron microscope, or a combination thereof. The sensitivity of detection for the method is at least as low as 10 colony forming units (cfu) of the pathogens in one milliliter of solution within one hour.
Owner:THE HONG KONG UNIV OF SCI & TECH +1

Formula and method for immobilizing tick sample for scanning electron microscopy

The invention relates to a formula and method for immobilizing a tick sample for scanning electron microscopy, which belongs to the technical field of methods for immobilizing samples for scanning electron microscopy. The immobilization formula comprises a PBS buffer solution, polysorbate and glutaraldehyde, and a glutaraldehyde immobilization liquid is prepared from the above-mentioned components. The method comprises the following steps: (1) observing whether host tissue is left in basis capituli of the tick sample and if so, removing the host tissue; (2) cleaning the surface of the tick sample; (3) immobilizing the tick sample at room temperature by using the glutaraldehyde immobilization liquid; (4) carrying out gradient dehydration with ethanol on the immobilized tick sample; (5) putting the tick sample into mixed liquor of absolute ethyl alcohol and acetone for displacement, and then putting the tick sample into acetone for displacement; and (6) drying the tick sample in a vacuum freeze drying instrument for drying and spraying gold on the tick sample. During scanning electron microscopic observation, the phenomenon of fuzzy and foggy images or incapable imaging due to ionization discharging of the sample as water vapor produced after electron beam bombardment of the sample encounters high-energy electron streams is prevented.
Owner:XINJIANG AGRI UNIV

Hydrothermal synthetic method for coating carbon nanometer tube with molybdenum sulphide

Compound nano pipes coated by molybdenum sulphide are produced by water heating synthesis, including: first to put the molybdate and thiourea in the water and the mass percent of molybdate is 0.2%-1.5%, the thiourea is 0.2%-2.0%, then disperse the nano pipes of C in the solvent by the super sonic wave, and the mass percent of it is 0.1%-0.5%, the mixture is then put into the non-corrosive steel tank which is full of Teflon to react, at 200-250deg.C for 20-40h. The 2-6 phase of molybdenum sulphide obtained by the transmission electron microscopic and energy scattering analyze. They can be used to prepare high performance compound material of tribology, catalytic carrier, and microprobe. The process is simple, and it could avoid using the H2S.
Owner:ZHEJIANG UNIV

Circulating microfluidic pump system for chemical or biological agents

A miniature pump has at least one controllable expansion-and-contraction chamber, and associated pair of tiny ducts interconnecting a fluid source and destination. The ducts communicate with the chamber(s); an linking tunnel links the ducts. Valves interact with fluid pressures due to expansion and contraction, imposing directionality on flow in the ducts and tunnel. Preferences: making the valve a passive flapper, implanting the pump in a creature, making the source a medication reservoir for supplying the creature; making the source a fuel tank and destination a tiny engine; making the source provide a specimen for assay and destination an observation slide; human or automatic examination of the slide under a microscope (e. g. electron microscope); making the source a reagent and destination a process stream; making the source a colorant and destination a colorant application system. Preferably included is an optical channel with intersecting fluid duct for optically monitoring pumped fluid.
Owner:KANE DAVID +1

Method for producing sintering plate-like corundum by ultra-high temperature shaft kiln

The invention discloses a method for producing sintered plate corundum by using ultra- high temperature shaft furnace. It employs low- sodium high- purity pre-sintered aluminum oxide powder Al2O3>98.5% as raw material, grinding, ball forming and drying; feeding ball material into high- temperature shaft furnace and calcining at 1850- 1920 Deg. C, keeping temperature for 1- 1.5 hours, ventilating and cooling for two times to make the temperature of ball material at discharge port be reduced to 80 Deg. C, disintegrating sintered ball material, sifting and getting final product. The invention is characterized in that the crystal size of plate corundum is 20- 200 um, activity is high, and the product quality are very good: density (g / cm3): 3.58- 3.62, porosity(%) is less than 5, water absorption rate (%) is less than 1.5; and the electron microscopic structure is: crystal size is 0- 200 um, and the crystal is equipped with close pores less than 10 um, and the crystal structure is compact.
Owner:HANZHONG QIYUAN NEW MATERIAL

Positive electrode active material for nonaqueous electrolyte secondary battery

A positive electrode active material for a nonaqueous electrolyte secondary battery includes particles of a lithium-transition metal composite oxide that contains nickel in the composition thereof and has a layered structure. The particles have an average particle size DSEM based on electron microscopic observation in a range of 1 μm to 7 μm in which a ratio D50 / DSEM of a 50% particle size D50 in volume-based cumulative particle size distribution to the average particle size based on electron microscopic observation is in a range of 1 to 4, and a ratio D90 / D10 of a 90% particle size D90 to a 10% particle size D10 in volume-based cumulative particle size distribution is 4 or less.
Owner:NICHIA CORP +1

Method for searching blind ore deposit by utilizing nanometer particles in organism

The invention discloses a method for searching a blind ore deposit by utilizing nanometer particles in an organism. The method comprises the steps of sampling, treatment and analysis of organism samples and judgment of analysis results, namely whether a to-be-tested area has the blind ore deposit is judged according to the characteristics of the analyzed particles, and ore elements are further predicted, wherein the sampling comprises getting animal and plant tissue samples in the to-be-tested area and storing in a fixing solution; the treatment comprises successfully attaching the animal and plant samples to a transmission electroscope carrying screen directly applied to a test on the premise of not damaging the samples; the analysis comprises detecting and analyzing the nanometer particles in the organism tissues on the carrying screen by adopting a transmission electron microscope. According to the method disclosed by the invention, by detecting and analyzing the nanometer particles in the organism tissues, whether a deep blind ore deposit exists or not can be accurately indicated, and the characteristics of the ore elements of a deep ore can be reflected directly. By utilizing the method in combination with the application of other physical and chemical survey techniques, the success rate for searching the ores can be effectively increased and the cost can be lowered as well.
Owner:SUN YAT SEN UNIV

Nickel-titanium amorphous alloy grid supporting film for transmission electron microscope

The invention discloses a nickel-titanium amorphous alloy grid supporting film for a transmission electron microscope and a preparation method of the nickel-titanium amorphous alloy grid supporting film. A conductive film used is a nickel-titanium alloy nanometer film; the nickel-titanium alloy is shape memory alloy which is high in elasticity and mechanical strength, serves as a good conductor for power and heat and can be widely applied to medical apparatuses and instruments. The nickel-titanium alloy is in a large-scale industry at present and is the alloy material easily obtained. The grid supporting film is prepared from the nickel-titanium alloy material, and thus the superelasticity, the high mechanical strength and the outstanding conductivity of the shape memory alloy can be fully utilized to problems of low mechanical strength and serious electric charge of a carbon supporting film can be solved.
Owner:PEKING UNIV

Electron microscopic observation method for observing biological sample in shape as it is, and composition for evaporation suppression under vacuum, scanning electron microscope, and transmission electron microscope used in the method

Provided is an observation method by an electron microscope, in which a biological sample can be observed as it is alive and a situation that the biological sample is moving can be observed using an electron microscope, and a composition for evaporation suppression under vacuum, a scanning electron microscope, and a transmission electron microscope used in the method.The sample observation method by an electron microscope according to the invention includes applying a composition for evaporation suppression containing at least one kind selected from an amphiphilic compound, oils and fats, and an ionic liquid to the surface of a sample to form a thin film, and covering the sample with the thin film, and displaying an electron microscopic image of the sample, which is covered with the thin film and accommodated in a sample chamber under vacuum, on a display device.
Owner:JAPAN SCI & TECH CORP

In vitro three-dimensional culture model of glioma stem cells and application thereof

ActiveCN104312975AEasy to operateTube-like structure is reliableBiological testingTumor/cancer cellsStainingWestern blot
The invention belongs to the field of cytobiology and specifically relates to an in vitro three-dimensional culture model for research on vasculogenic mimicry of glioma stem cells and an application thereof. Preparation of the in vitro three-dimensional culture model comprises the following steps: immersing a three-dimensional collagen scaffold in a DMEM culture medium for 6-24 h, taking out the three-dimensional collagen scaffold, removing superfluous DMEM adhered to the three-dimensional collagen scaffold to obtain a pretreated three-dimensional collagen scaffold, placing the pretreated three-dimensional collagen scaffold on a cell culture plate, culturing sphere-formed glioma stem cells in the pretreated three-dimensional collagen scaffold, standing at 37 DEG C for 2-6 h, adding an endothelial medium, and culturing at 37 DEG C for 2-4 days, so as to obtain the in vitro three-dimensional culture model for research on vasculogenic mimicry of glioma stem cells. Each operational process of the in vitro three-dimensional culture model is carried out at room temperature; various kinds of in situ staining, such as immunohistochemical staining and immunofluorescent staining, can be carried out; electron microscopic examination can be carried out; and RT-PCR and Western Blot detection can be carried out directly after digestion.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA

Chromium hydroxide, method for producing the same, trivalent chromium-containing solution using the same, and chromium plating method

Disclosed is a chromium hydroxide which is characterized by having an average particle diameter D of 40-200 nm and an aggregation degree, which is represented by the ratio D50 / D between the volume average particle diameter D50 measured by a particle size distribution measuring instrument and the average particle diameter D determined by a scanning electron microscopic image, of not less than 10 but less than 70. This chromium hydroxide is suitably obtained by adding an aqueous solution containing trivalent chromium into an inorganic aqueous alkaline solution under such a condition that the temperature of the reaction liquid is not less than 0 DEG C but less than 50 DEG C. A hydroxide of an alkali metal is preferably used as the inorganic alkali.
Owner:NIPPON CHECMICAL IND CO LTD +2

Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof

The invention discloses a bdellovibrio bacteriovorus bacterial strain for eliminating listeria monocytogenes and an application thereof. The invention obtains bdellovibrio bacteriovorus BDSM08 by separation, purification and ultraviolet mutation. BDSM08 is in the arc-shaped unicellular form when observed by an electron microscope, the size thereof is 1.8*1.0 mu m, the ends thereof are provided with flagellum and the length of the flagellum is 3.2 mu m. The bdellovibrio bacteriovorus BDSM08 is cultivated with the two layer plating method at the temperature of 28 DEG C for three days to form transparent round negativecolony the diameter of which is 2-3mm and the optimum growing pH value of which is 7.2, the optimum growing temperature of which is 28 DEG C and the optimum salinity of which is0%. The microbial agent prepared by BDSM08 has a strong effect on killing listeria monocytogenes which can be commonly seen in environment. The invention applies the bdellovibrio bacteriovorus BDSM08for the first time to prevent and cure food listerellosis; the invention has a favourable effect on eliminating listeria monocytogenes on food, has characteristics of no toxicity, no side effect on food and the like, and has favourable application prospect.
Owner:SOUTH CHINA UNIV OF TECH

Method for analyzing pore structure of organic matter in Lower Paleozoic shale

The present invention discloses a method for analyzing the pore structure of the organic matter in the Lower Paleozoic shale. The method comprises the following steps: performing optical scale and two-dimensional scanning electron microscopic observation, performing three-dimensional cutting reconstruction by selecting typical organic matters, and performing detailed observation and image acquisition on the pore structure of the target organic matter; and using image analysis software to quantitatively characterize the characteristic parameters such as the face rate, the pore size distribution, the porosity and the like of different organic matters. According to the method provided by the present invention, the pore morphology of the organic matter of the Lower Paleozoic shale can be accurately and reliably analyzed, so that the problem of quantitative analysis on the pore structure of the organic matter can be solved; and the method has important guiding significance for the study onthe pore structure of the organic matter in the Lower Paleozoic shale, and provides a better reference for the study of the organic matter in the Lower Paleozoic shale and the exploitation of the Lower Paleozoic shale gas.
Owner:CHONGQING INST OF GEOLOGY & MINERAL RESOURCES

Preparation method of transmission electron microscopic sample

The invention provides a preparation method of a transmission electron microscopic sample. The method comprises the following steps: providing a sample with length, width and thickness, wherein the sample has an observation target, and the observation target includes at least one elongation structure along a thickness direction or a multilayer stack structure; marking the surface of the sample defined in the length and width directions, wherein a distance from the marked position to the observation target is in a preset distance ring; grinding the sample along a direction vertical to the thickness direction to the marked position; arranging the sample on a supporting desk, and allowing the grinding surface of the sample to depart from the supporting desk and the opposite surface of the grinding surface to face the supporting desk; forming a conductive film on the back of the sample in order to electrically connect the sample with the supporting desk; and processing the grinding surface by using focused ion beam to obtain the observation sample containing the observation target. The method improves the preparation efficiency, improves the hit rate, and avoids the problem of imaging blurring or image drift induced by charge accumulation in the sample preparation process.
Owner:SEMICON MFG INT (SHANGHAI) CORP

Comfortable sample double spray electrolytically thinning instrument for transmission electronic microscopy

An electrolytic thickness reducer is composed of DC power, electrolytic device of magnetic agitation and double spray and illumination device. It is featured as using 0-36 V span for DC power, placing a p-m pole in double spray component set in outer organic box with electrolyte, using stainless steel sheet as negative pole, placing positive sample in between two nozzles, having electron microscopic sample conducted with phi 0.3 mm Pt wire and stainless steel bar and having parallel light conducted out by all reflecting cable.
Owner:UNIV OF SCI & TECH BEIJING

Sample supporting member for observing scanning electron microscopic image and method for observing scanning electron microscopic image

When injection of electrons into a sample supporting member causes a potential gradient between an insulative thin film and a conductive thin film at a site of electron beam injection, the potential barrier of the surface of the insulative thin film becomes thin, and an electron emission phenomenon is caused by tunnel effects. Secondary electrons caused in the insulative thin film tunnel to the conductive thin film along the potential gradient. The secondary electrons, having tunneled, reach a sample while diffusing in the conductive thin film. In the case where the sample is a sample with a high electron transmittance, such as a biological sample, the secondary electrons also tunnel through the interior of the sample. The secondary electrons are detected to acquire an SEM image in which the inner structure of the sample is reflected.
Owner:NAT INST OF ADVANCED IND SCI & TECH

Surface-treated fluorescent bodies and process for production of surface-treated fluorescent bodies

The present invention aims to provide a surface-treated phosphor having high dispersibility and capable of significantly enhancing moisture resistance without deteriorating the fluorescence properties, and a method for producing the surface-treated phosphor. The surface-treated phosphor includes: a phosphor body; and a surface treatment layer containing at least one specific element selected from elements of the third to sixth groups of the periodic table, and fluorine, the phosphor body having the surface treatment layer on the surface thereof, wherein, when a cross section of the surface treatment layer is subjected to a thickness-wise elemental distribution analysis by a combination of an electron microscopic analysis and an energy-dispersive X-ray element analysis, a peak indicating the maximum content of the specific element appears nearer to the surface than a peak indicating the maximum fluorine content.
Owner:SEKISUI CHEM CO LTD

Method for observing in a plant blade microstructure in an oriented manner by treating blade

ActiveCN102967497AOvercome disengagementOvercoming the problem of leaf curlingWithdrawing sample devicesPreparing sample for investigationMicro structureSpatial positioning
The invention relates to a method for observing in a plant blade microstructure in an oriented manner by treating a blade by utilizing a large-precise instrument-transmission electron microscope. The method is characterized in that a blade section structure chart correlated to a transverse slice is drawn by using a new process, the positioning of the transverse slice in a tissue structure and a spatial structure of the blade is realized, and the complete set of precise positioning structure chart of the blade with a complicated microstructure or the precise positioning structure chart of a certain specific part can be guaranteed and obtained by using a set of spatial positioning rules; and a microcosmic stereostructure can be established by combining the blade section structure with a transverse orderly positioning structure. The invention aims at providing a brand new sample treating, slicing, positioning and observing method utilizing the transmission electron microscope to observer the blade structure as the transverse slice is taken as a basis, and according to method, the operation is simple, the precise positioning research can be carried out on all the microstructures of the blade; and simultaneously, the true microcosmic stereostructure of the blade is detected by the orderly positioning structure.
Owner:SHENYANG AGRI UNIV

Electron microscopic imaging system and imaging method

The invention discloses an electron microscopic imaging system and an imaging method, and belongs to the field of electron imaging. The electron microscopic imaging system comprises a plurality of charged particle sources, wherein the charged particle sources are used for emitting charged particles; a convergence unit located under the charged particle sources; a diffraction unit located under theconvergence unit, a sample being arranged between the diffraction unit and the convergence unit; and a detector located under the diffraction unit. According to the method, the plurality of charged particle sources emit charged particles to form a plurality of charged particle beams; the plurality of charged particle beams pass through the convergence unit to form a charged particle beam probe; the charged particle beam probe penetrates through the sample and forms a diffraction pattern of the sample through the diffraction unit; and the detector receives the diffraction pattern and performsstacked imaging to reconstruct a sample image. The system aims to overcome the defects that when a single electron beam is used as an electron beam probe, the imaging quality is reduced and the data acquisition efficiency is reduced due to frequent movement; and the data acquisition efficiency and the imaging quality can be improved.
Owner:NANJING UNIV

Measurement method of electron microscopic image line width and roughness

The present invention belongs to the scanning electron microscopic measurement technology field, and discloses a measurement method of the electron microscopic image line width and roughness. The method comprises the steps of obtaining a scanning electron microscopic image of a to-be-measured line structure; intercepting a first area; processing averagely along a line direction, and obtaining a line edge pixel distribution curve; according to the line edge pixel distribution curve, determining a first boundary area; analyzing the local pixels, and obtaining the boundary distribution; according to the boundary distribution, calculating the width and the roughness of a to-be-measured line, and extracting the width and roughness numerical values of the to-be-measured line. The measurement method of the present invention solves the problems in the prior art that the workload of measuring the line width and roughness is larger, a measurement error caused by the human intervention exists, and a limited number of data points only can be analyzed, and realizes the technical effects of improving the measurement accuracy and reliability, saving the actual measurement time and cost of the engineers.
Owner:INST OF MICROELECTRONICS CHINESE ACAD OF SCI

Novel three-dimensional reconstruction system

A novel three-dimensional reconstruction system comprises a workflow presenting module of an Appion system, a data instruction conversion module, a data configuration module, a grid job queue module of an LSF system, an LSF scheduling library module, an LSF billing library module and a network resource pool module. The novel system based on the LSF system, the Appion system and a cryo-electron microscopy technology achieves three-dimensional reconstruction. The system ingeniously and organically combines the Appion system, a cryo-electron microscopy biological structure research technology and a grid calculation technology based on an LSF, and forms a novel cryo-electron microscopy three-dimensional reconstruction technology. The technology is mainly characterized by achieving a three-dimensional reconstruction assembly line technology, and the assembly line technology can effectively reduce work intensity of electron microscopic image analysis of biologic samples and effectively improve research efficiency of an electron microscopy three-dimensional reconstruction method in structural biology.
Owner:TSINGHUA UNIV

Pellicle and mask adhesive therefor

Disclosed is a pellicle having a mask adhesive layer having appropriate softness, having a small adhesive residue after being peeled off from a mask, and having good handling characteristics; and a pellicle for preventing position deviation of patterns, in particular in double patterning. The pellicle of the present invention includes a pellicle frame, a pellicle membrane disposed on one end surface of the pellicle frame, and a mask adhesive layer disposed on other end surface of the pellicle frame; wherein the mask adhesive layer includes 35 to 170 weight parts of a hardness adjuster (B) containing a polypropylene (b1) and a propylene based elastomer (b2) per 100 weight parts of a styrene resin (A); and in an electron microscopic photograph of the mask adhesive layer, a phase-separated structure of a continuous phase of the styrene resin (A) and a discontinuous phase of the hardness adjuster (B) is observed.
Owner:MITSUI CHEM INC

Scanning electron microscopic imaging method

The invention discloses a scanning electron microscopic imaging method, and belongs to the technical field of microscopic imaging. The scanning electron microscopic imaging method comprises the stepsof scanning the target object under the diaphragms with different convergence angles by using a scanning electron microscope to obtain a diffraction pattern; and mixing the diffraction patterns corresponding to the diaphragms to obtain a mixed diffraction pattern, performing multiple iterative calculations according to the mixed diffraction pattern to reconstruct O (r), taking the Oz (r) reconstructed by final iterative calculations as a final complex amplitude distribution function of the target object, and obtaining a reconstructed target object image according to the amplitude and phase ofthe Oz (r). The method aims to overcome the defects in the prior art. In order to overcome the defect that a diffraction pattern generated by a single diaphragm in a stacked imaging method can only obtain a reconstructed image of part of frequency band information of a target object, the invention provides a scanning electron microscopic imaging method which can obtain high-frequency characteristics and low-frequency characteristics of the target object, so that a clear and complete target object image can be obtained.
Owner:NANJING UNIV
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