70 results about "Electron microscopic" patented technology

The present invention discloses a method for batch preparation of graphene electron microscope grids. The present invention realizes high-quality batch transfer of graphene and electron microscope grids by designing a multi-level transfer medium (including a PDMS porous template, a polymer support layer and a small molecule buffer layer) in combination with a solvent volatilization-induced elastic capillary bonding process. Specifically, the surface of the graphene is uniformly coated with a small molecule / polymer transfer medium layer, and then bonded to the PDMS porous array template. After the graphene is peeled off from the growth substrate, the graphene supported by the polymer film in the suspended part of the PDMS is bonded to the electron microscope grid using a solvent thermal bonding method; finally, the transfer medium is removed to obtain the graphene grid. The method of the present invention has the advantages of simple process, reusable PDMS template and good compatibility, and is suitable for large-scale production. At the same time, the prepared graphene grid has high cleanliness and integrity, and can meet the application requirements in the field of cryo-electron microscope grids.

Methods and systems for calibrating a transmission electron microscope are disclosed. A fiducial mark on the sample holder is used to identify known reference points so that a current collection area and a through-hole on the sample holder can be located. A plurality of beam current and beam area measurements are taken, and calibration tables are extrapolated from the measurements for a full range of microscope parameters. The calibration tables are then used to determine electron dose of a sample during an experiment at a given configuration.

The invention provides a combined type electronic detector, electronic detection equipment and a detection method, and belongs to the technical field of electronic microscopic imaging. The combined type electronic detector adopts a structure that yttrium aluminum garnet crystals are arranged to partially cover a split type silicon diode detector, so that physical replacement of any detection body is not needed, and the detection efficiency is improved. According to the invention, signals of different areas can be selected and read through an electronic mode, so that an optimal detection mode is called under different working conditions, detection can be realized under the condition of high acceleration voltage, detection can also be realized under the condition of low acceleration voltage, one machine with two purposes is realized, and the performance and the application range of electron beam equipment are greatly improved.

The invention relates to a low-stress chemical mechanical etching process suitable for fragile materials, which belongs to the technical field of etching processing of fragile materials and comprises a precise chemical mechanical polishing machine, a polishing pad, a polishing solution conveying and managing system and end point detection equipment. The precise chemical mechanical polishing machine comprises a multi-zone pressure controllable polishing head, a high-precision rotating speed control system and a real-time thickness measuring system, a polishing pad is a polishing disc made of porous polyurethane materials, and the polishing liquid conveying and managing system comprises a multi-channel metering pump system and a stirring and temperature control unit. And the end point detection equipment comprises a surface profilometer, an atomic force microscope and a transmission electron microscope. According to the method, through fine cooperation of chemical reaction and mechanical grinding, microcrack initiation and propagation in the polishing process are effectively inhibited, surface and subsurface damage of the fragile material is remarkably reduced, the machined surface which is ultrahigh in smoothness and free of damage is obtained, and meanwhile the high material removal rate is kept.

The invention discloses an efficient debugging preparation method of a DFB laser grating. The method comprises the following steps: obtaining the width and depth of a grating line through electron microscopic imaging to form an initial parameter set; comparing design parameters to identify an abnormal area; analyzing a spatial distribution rule of abnormal region parameters, and classifying process fluctuation types; performing selective filtering on the geometric parameter sequence according to the fluctuation type to obtain a correction parameter; evaluating the influence on the output wavelength according to the correction parameter, and generating a process compensation coefficient when the deviation is predicted; predicting and optimizing structural parameters through process simulation; and finally generating a control instruction to drive the etching equipment to adjust the process in real time, and determining that the performance reaches the standard through monitoring. According to the invention, the fluctuation source can be intelligently diagnosed, the parameters are accurately corrected, closed-loop control is realized, and the anti-interference capability, control precision and efficiency of the preparation process are effectively improved.

The invention discloses an intelligent analysis system and method for an electron microscopic image crystal structure, and the system comprises a data importing module which is used for importing an electron microscopic image and crystal structure data; the image preprocessing module is used for preprocessing the imported electron microscopic image to obtain an experimental map; the diffraction simulation module is used for simulating two-dimensional electron diffraction of the crystal based on the imported crystal structure data to obtain a diffraction simulation result, and processing the diffraction simulation result to obtain a theoretical map; the intelligent matching module is used for matching the experimental map with the theoretical map and outputting a matching result; and the result output module is used for outputting the matching result and the diffraction simulation result to realize the visualization of the crystal structure. Under the synergistic effect of all the modules, the crystal structure of the electron microscopic image can be automatically analyzed in the whole process, and the analysis efficiency of the crystal structure is greatly improved; and meanwhile, under the action of the intelligent matching module, rapid matching can be realized, and the misjudgment rate is reduced.

The invention discloses a flexible particle deformation detection method and system based on deep learning. The method and system are suitable for classification of two-dimensional particles in a Cryo-EM image. For the problems of low signal-to-noise ratio, complex particle deformation and the like in a Cryo-EM image, noise reduction processing is performed on an original image through methods of Topaz, low-pass filtering, non-local mean filtering and the like, so that the structural distinguishability is improved; then, carrying out binarization and connected region detection to obtain a particle contour, and generating a corresponding two-dimensional point cloud expression; in order to realize efficient registration, a KMP heuristic point cloud matching algorithm is provided, and the corresponding relation between the particle point cloud and the template point cloud is effectively established; on the basis of matching, continuous deformation tracks of the simulated particles are predicted through multi-wheel displacement, detection of deformation values is achieved, and the deformed particles are removed to achieve the classification effect. According to the method, the precision and robustness of flexible structure modeling are improved while the point cloud matching efficiency is improved, and the method is suitable for a particle structure analysis task under a high-noise condition.

The utility model belongs to the technical field of medical instruments, and particularly relates to an on-site rapid pathology operation platform which comprises a machine frame, a base, universal wheels arranged at the four corners of the bottom of the base, a box body arranged at the top of the base, a workbench arranged at the top of the box body and a conveying mechanism arranged at the top of the workbench. The flaking assembly comprises a bracket arranged on one side of the top of the workbench, a coloring agent storage tank arranged on the top of the bracket and a dyeing spray head arranged at the bottom of the coloring agent storage tank, and the dyeing spray head is located over the conveying mechanism; the film reading assembly comprises a hinge frame arranged on the other side of the top of the workbench, an electron microscope arranged on the hinge frame and a carrying plate arranged on the top of the workbench, and the carrying plate is located under the electron microscope; the novel film reading device is reasonable in structure, can be used for quickly preparing and reading films on site in the use process, is beneficial to improving the diagnosis efficiency, and can reduce the working intensity of medical personnel.

A positioning system for an electron microscope, comprising: a first carriage and a second carriage, the first carriage comprising a holder for holding a workpiece. The first carriage is coupled to one or more first drive units configured to position the workpiece along a first axis, a second axis, and a third axis and along a first tilt axis. The second carriage houses the one or more first drive units and is coupled to one or more second drive units configured to position the workpiece along a second tilt axis.

The utility model discloses a temperature control loading device for a scanning electron microscope, which relates to the technical field of microscopes and comprises a sample chassis, a cooling chamber is fixedly mounted on the sample chassis, a cooling cover is mounted at the top of the cooling chamber in a threaded manner, and an objective table is fixedly mounted on the top surface of the cooling cover. A plurality of air guide holes are uniformly formed in the cooling cover, a temperature sensor is arranged on the objective table, and a temperature control assembly is arranged in the cooling cover. The temperature of the objective table is detected through the temperature sensor and is fed back to a control system of the scanning electron microscope for judgment; if the biological sample is about to exceed the cooling temperature range required by the biological sample, the face gear can be driven to rotate by the driving motor, and the threaded rod can be driven to rotate by the meshing straight gear, so that the baffle can slide along the sliding chute to gradually shield or open the air guide hole, and the cooling temperature is controlled by controlling the size of the air guide hole; the device is suitable for different biological samples.

The current invention relates to an improved microfluidic device for protein / protein complex purification, as well as an associated methodology and system.In particular it relates to systems and methods for electron microscopy, preferably cryogenic electron microscopy (cryo-EM), on protein samples, comprising: a. at least one microfluidic chip with inlet and outlet and purification device; b. at least one illumination means and detection means for, preferably fluorescence, measurements on the microfluidic chip; c. a pumping system adapted for operable connection to the microfluidic chip, and configured for controlling flow in the operably connected microfluidic chip; d. a microscopy grid, preferably a cryogenic electron microscopy (cryo-EM) grid, for holding fluid samples; e. preferably a cryogenic container, for a cryogenic coolant; f. preferably a transport system for moving the cryo-EM grid between a position for receiving a fluid sample from the microfluidic chip and the cryogenic container; g. a control system, preferably a processor, which receives information on the measurements from the detection means, configured for controlling the pumping system at least based on said information, and further configured for controlling the illumination and detection means and preferably also for controlling the transport system.

The invention discloses a bulk electron microscopic image splicing method based on a multi-scale image pyramid, and belongs to the technical field of image processing. According to the method, two-stage local abnormal value filtering and RANSAC enhancement based on local geometric constraints are introduced in the image registration stage, and image enhancement, feature re-extraction and multi-iteration TPS displacement field calculation are adopted in local deformation correction, so that the adaptability and robustness of the algorithm to a complex biological sample microstructure and potential deformation are jointly improved. By introducing a multi-scale image pyramid structure and a hierarchical processing strategy, a complex fusion problem of a high-resolution image is decomposed into a plurality of low-resolution sub-problems, and the calculation efficiency and the memory utilization rate are remarkably improved; and the problem of fuzzy texture details caused by a traditional weighted average method is effectively avoided.

The utility model relates to the technical field of clamping, in particular to a clamping device for detecting a sample, which comprises a base and a clamping mechanism, a rectangular groove is arranged in the middle of the base, and test paper for detecting the sample is placed in the rectangular groove; the clamping mechanism comprises a clamping cover plate and a positioning block, four inserting grooves are formed in the top of the base and located in the outer side of the side length of the rectangular groove, and the positioning block is arranged at the bottom of the clamping cover plate and matched with the inserting grooves. Clamping blocks are telescopically arranged on the side walls of the positioning blocks, clamping grooves are formed in the side walls of the inserting grooves, and the clamping blocks are clamped and matched with the clamping grooves; a pressing block is arranged at the bottom of the clamping cover plate and is used for pressing corners of the test paper of the detection sample. According to the utility model, the displacement of the test paper sample in the observation process of the electron microscope due to vibration and the like can be effectively prevented, and the accuracy of the observation result is ensured.

The invention discloses cyclodextrin-mediated water-soluble rare earth nanoparticles and a synthesis method and application thereof in antibody coupling, and the method comprises the following steps: preparing uniform rare earth nanoparticles with different particle sizes by using a high-temperature thermal decomposition method, and carrying out self-assembly modification by using polyethylene glycol (PEG) derivative modification and carboxylated cyclodextrin (CM-CD) to obtain the cyclodextrin-mediated water-soluble rare earth nanoparticles. And coating the surfaces of the rare earth nanoparticles with PEG chain molecules or PEG (at) CM-CD composite molecules to obtain the modified water-soluble rare earth nanoparticles. And then mixing the modified water-soluble rare earth nanoparticles with 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS), introducing NHS ester, and carrying out covalent coupling with an antibody to obtain the antibody-coupled rare earth nano probe. An electron microscope, a zeta potential test and an enzyme-linked immunosorbent assay detection kit test prove that the water solubility and the dispersity of the rare earth nanoparticles can be improved by carboxylated cyclodextrin modification, and meanwhile, the loading capacity and the affinity of a coupling antibody are improved.

1. Name of the product in this design: Body Fluid Detector (HCY-JCH-A). 2. Purpose of this design: To detect bodily fluids and realize the function of an electron microscope. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

A method of sample preparation in association with an electron microscope. In one embodiment, a carrier supporting a biological sample is received. A high-pressure freezing (HPF) protocol is then applied to the carrier. The protocol comprises a first phase, and a second phase that follows the first phase. The first phase comprising (i) comprising: a pressurizing operation applying a pressure to the carrier, and (ii) a rapid cooling operation using a coolant that avoids a Leidenfrost effect at a carrier-coolant interface by maintaining nucleate boiling or substantially suppressing film boiling upon contact with the carrier. The pressurizing and rapid cooling operations freeze the biological sample while preserving its structural integrity. The second phase involves raising a temperature of the carrier and the frozen biological sample to a given temperature for one of: storage, and use.

The utility model discloses a portable pulse imaging cell recognition device which comprises a bottom plate, a mounting plate is hinged to one side of the bottom plate, a placement groove is formed in the inner wall of the mounting plate, a display operation panel is hinged to the inner wall of the placement groove, and a supporting plate is hinged to the outer wall of one side of the display operation panel. Clamping grooves matched with the supporting plates in a limiting mode are formed in the inner walls of the containing grooves, a plurality of inserting grooves are formed in the outer wall of the top of the bottom plate, inserting blocks are inserted into the inner walls of the inserting grooves, hand screwing bolts matched with the inserting blocks in a limiting mode are in threaded connection with the inner walls of the inserting grooves, side plates are connected to the top ends of the inserting blocks, and convex edges are fixedly connected to the tops of the side plates and the tops of the mounting plates. The outer walls of the multiple convex edges are in sliding fit with the same sealing cover. By arranging the mounting plate, the supporting plate, the convex edge, the slot, the side plate, the insertion block and the sealing cover, the electron microscope main body, the bearing assembly and the display operation panel can be folded into a rectangular whole, so that the device is convenient to carry.

The invention discloses a high-throughput screening method for TMV (Tobacco Mosaic Virus) resistant microorganisms. The high-throughput screening method comprises the following specific steps: (1) purifying TMV; (2) activation of a to-be-detected strain: inoculating a single colony into a liquid culture medium, culturing on a constant-temperature shaking table under the conditions of 28 DEG C and 180 r / min for 24-48 hours, and performing co-incubation of the to-be-detected strain and the virus when the OD600 value is within the range of 0.6-1; (3) co-incubation: culturing the bacterial liquid to be detected and viruses on a constant-temperature shaking table for 0-3 days under the conditions of 28 DEG C and 180r / min, and taking 1mL of co-incubation liquid every 24 hours for a subsequent antiviral activity detection test; and (4) antiviral activity detection: detecting the virus concentration after co-incubation by adopting methods such as Elisa, an electron microscope and RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction). According to the invention, the screening efficiency is improved, microorganisms or compound resources with tobacco mosaic virus resisting activity are quickly found, and a new solution is provided for agricultural disease control.

The invention relates to the field of film adhesive force measurement, in particular to an adhesive force measurement method and system for an OSP organic solderability preservative film, and the method comprises the steps: detecting the surface of the OSP organic solderability preservative film to be measured by using an electron microscope SEM for preliminary evaluation; independently sampling the to-be-measured OSP organic solderability preservative which passes the preliminary evaluation to obtain an OSP organic solderability preservative sample group; respectively selecting one sample from the OSP organic solderability preservative sample group, and carrying out scratch test, thermal cycle test and tensile test; the critical load obtained by the scratch test, the film layer change condition obtained by the thermal cycle test and the adhesive force strength data obtained by the tensile test are systematically and comprehensively analyzed, and by constructing a mathematical model, various data are weighted and calculated to obtain an adhesive force measured value. According to the invention, the adhesive force of the OSP organic solderability preservative is evaluated through comprehensive dimensions, and the accuracy and reliability of the measurement of the adhesive force of the OSP organic solderability preservative are improved.

This invention relates to the field of stem cell culture technology, and particularly to a stem cell culture device and its method of use, comprising a housing, a base plate, and a cover plate. An annular observation window is provided on the cover plate, and a detection mechanism for observing stem cells is also provided on the cover plate. By positioning the movement trajectory of the sample carrier, liquid level fluctuations caused by tilting of the culture flask during ascent are effectively avoided. Combined with the pressure regulation function controlled by the micro-flow one-way valve of the telescopic damping airbag, stable and uniform lifting and lowering of the sample carrier component is achieved. With the assistance of annular scanning by an electron microscope, multiple batches of samples can be tested without manual intervention. This effectively avoids the acceleration impact caused by traditional sample transfer methods, ensuring the stability of the stem cell suspension and effectively preventing the impact of mechanical vibration on stem cell growth, thereby effectively improving the accuracy and repeatability of stem cell detection data.

The invention discloses an ultralow-temperature freezing sample rod of a transmission electron microscope, and belongs to the low-temperature technical field of transmission electron microscopes. The sample rod comprises a grip, a rod body, a liquid nitrogen precooling tank and a sample bearing assembly; a heat exchange channel is integrated in the liquid nitrogen precooling tank; a coaxial heat exchanger, a shunting chamber and an expansion chamber are arranged in the rod body, a pipeline connected with a cold source and a heat exchange channel is arranged in the grip, the heat exchange channel is further connected with an inner pipe of the coaxial heat exchanger, an outer pipe of the coaxial heat exchanger is hermetically connected with the grip and the shunting chamber respectively, the inner pipe penetrates through the shunting chamber and is connected with the expansion chamber, and the shunting chamber is connected with the expansion chamber. The end of the expansion chamber is hermetically connected with the sample bearing assembly; an expansion valve is arranged at the end, connected with the expansion chamber, of the inner pipe and used for cooling and depressurizing gas entering the expansion chamber through the expansion valve. The flow dividing chamber is used for dividing the cooled and depressurized gas input through the second pipeline, so that the flow speed of the gas is uniform. The method is suitable for extremely-low-temperature in-situ research.

Apparatus and methods are disclosed for a mechanically stable, long-life, cold field emitter assembly which is compatible with ultra-high vacuum and occasional high-temperature flashing. A metal adapter is welded between a hexaboride electrode and a metal filament. Some embodiments use a tungsten filament, a tantalum adapter, and a LaB6 microrod electrode with a nanorod emitter tip. Other material combinations are disclosed, as also usage in electron sources for electron microscopes. In variations, the adapter is deposited onto the filament and the electrode then welded to the adapter.

The application discloses a three-dimensional feature recognition method, device, equipment and storage medium. The method comprises the following steps: acquiring a first electron microscopic image; generating first frequency domain information corresponding to the first electron microscopic image; analyzing the first frequency domain information based on a three-dimensional feature recognition model to obtain feature data of a three-dimensional structure corresponding to the first electron microscopic image; and outputting the feature data of the three-dimensional structure corresponding to the first electron microscopic image. It can be seen that, by means of the three-dimensional feature recognition model, the first frequency domain information of the first electron microscopic image generated by the CD-SEM can be directly analyzed and processed, without additional hardware modification of the existing detection equipment, so that the detection efficiency of the feature data of the three-dimensional structure can be improved, and the detection cost can be reduced. Moreover, the feature data of the three-dimensional structure is captured in the frequency domain, so that the key features of the data can be more directly extracted, efficient and accurate feature recognition is realized, and the accuracy of micro-nano structure analysis is effectively improved.

The application provides a sample transfer device applied to a scanning electron microscope quick sample changing chamber, and belongs to the technical field of scanning electron microscope accessories, and aims to solve the problems of limited universality of the existing transfer device, incompatibility with the quick sample changing chamber and sample recovery difficulty. The device comprises a cylinder assembly, a limiting assembly and a pressure adjusting assembly. The cylinder assembly comprises a barrel box and a sample groove slider provided with a sample groove. Three sealing rings are arranged on the outer surface of the slider to form three sealed spaces in the box. The limiting assembly comprises a guide rod and a limiting plate, which is used for limiting the sliding-out distance of the slider, ensuring that the sample groove is completely exposed after sliding out and the second sealing ring is still located in the box. The pressure adjusting assembly comprises a locking screw and a sealing plug for adjusting the required air pressure for the sliding-out of the slider. The application is small in size, compatible with conventional sample chambers and quick sample changing chambers, can realize automatic sliding-out of the sample under vacuum and automatic sealing after testing, and is safe and reliable in operation.

The invention relates to an improved purification environment and pure bacterium source extracellular vesicle method, and relates to the technical field of biology, the method comprises the following steps: S1, obtaining a first crude extraction extracellular vesicle sample, carrying out exploratory multi-layer density gradient ultracentrifugation on the first sample, and carrying out characterization analysis on each centrifuged component, to determine an enrichment zone of extracellular vesicles; and S2, obtaining an extracellular vesicle sample of a second crude extraction homologous with the extracellular vesicle sample of the first crude extraction, constructing an optimized simplified density gradient according to the enrichment regions determined in the step S1, and carrying out optimized simplified density gradient ultracentrifugation on the second sample to collect and purify the extracellular vesicles. According to the method disclosed by the invention, a standardized scheme is established for a specific sample through two steps of exploratory purification and purification after optimization, and enrichment regions of the extracellular vesicles are determined by adopting an identification method combining a transmission electron microscope and nanoflow cytometry, so that the repeatability of a purification result is improved.

The present application provides a method for protein structure analysis using cryo-EM, comprising: including a tag in a target protein; combining the target protein including the tag with a scaffold protein to form a complex of the target protein and the scaffold protein; and using a cryo-EM for single particle imaging to analyze the structure of the target protein complexed with the scaffold protein. The scaffold protein is any one of streptavidin, avidin or a derivative thereof. The tag is used to selectively bind to the scaffold protein. The tag is selected from one of the following group: a biotin tag including biotin; a biotinylated protein or polypeptide tag including a protein sequence and biotin covalently linked to the protein sequence; a streptomyces tag (Strep-tag); and a biotinylated or streptococcal labeled antibody, antibody Fab fragment or single chain antibody.

An electrophotographic roller includes: a conductive substrate; and a foamed layer on an outer circumferential surface of the substrate, in which the foamed layer is a surface layer of the electrophotographic roller, the foamed layer has a skeleton including a binder resin, and at least one conductive particle in the binder resin, the foamed layer has a communication structure having at least one gap opening, and extending in a thickness direction of the foamed layer, at least a portion of an inner wall of the gap includes the skeleton, at least a portion of the at least one conductive particle is exposed at the inner wall, a thickness of the foamed layer is 2.0 mm or more, and in the observation of the cross section of the foamed layer by a scanning electron microscope, the ratio of number of pixels of a specific brightness falls within a specific range.

This invention belongs to the field of metallic material preparation technology, and specifically relates to a machine learning-based design method for interstitial element-strengthened titanium alloys in additive manufacturing. This machine learning-based design method includes: constructing a physical information-enhanced feature space; establishing an original dataset; augmenting the original dataset using data augmentation methods to establish an augmented dataset; using a multi-step feature screening strategy to determine key features affecting material properties; establishing a key feature-material property prediction model; using a multi-objective optimization strategy to collaboratively optimize multi-objective properties; and performing additive manufacturing to obtain a part; and using transmission electron microscopy to characterize the microstructure of the part. The design method provided by this invention can effectively solve the problems of high cost, long cycle, and low reliability in the composition and process optimization design of high-performance titanium alloys.

The invention belongs to the technical field of biological medicines, and particularly relates to application of FIS1 as a biomarker and a treatment target in NPM1 mutant leukemia. The mitochondrial fission enhancement phenomenon in the NPM1 mutant leukemia cell line is detected through a transmission electron microscope; then detecting the expression level of the mitochondrial fission gene by utilizing a qRT-PCR technology, and finding that the FIS1 is remarkably and highly expressed in the NPM1 mutant leukemia cell line; in addition, it is further verified that FIS1 is remarkably high in expression in clinical patients with NPM1 mutant leukemia through a western blot method, and the FIS1 can be used for indicating diagnosis of the disease. Subsequently, an in-vitro experiment further proves that the survival of NPM1 mutant leukemia cells can be remarkably inhibited by knocking down the expression of the FIS1. Therefore, a new target is provided for diagnosis, treatment and prognosis evaluation of the NPM1 mutant leukemia, and it is prompted that FIS1 has an important potential application prospect in diagnosis and treatment of the NPM1 mutant leukemia.

The invention discloses an inhibitor for inhibiting development of ampullaria gigas eggs and application of the inhibitor. Through medicament screening, a compound mixture of salad oil and acetic acid provided by the invention can effectively inhibit the development of ampullaria gigas egg masses, the optimal ratio of salad oil to acetic acid is 10: 1, and 0.07 g of medicament is applied to 1 g of eggs. Laboratory and field tests show that the inhibition rate of the mixture to ampullaria gigas egg masses reaches 100%. A scanning electron microscope is used for observing the processed ampullaria gigas ovum structure to show that more pores are formed on the surfaces of the ovum masses after the mixture is processed, so that the mixture can enter an embryo easily. Further respiratory action experiments show that the respiratory action of the egg masses treated by the mixture is obviously reduced within 24 hours, which is speculated that the mixture permeates into the egg masses, the respiratory action of the egg masses is inhibited, and the development of the eggs is further inhibited. The invention provides an environment-friendly, efficient and cheap technical means for prevention and treatment of ampullaria gigas.