41 results about "Electron microscopic" patented technology

The invention provides a combined type electronic detector, electronic detection equipment and a detection method, and belongs to the technical field of electronic microscopic imaging. The combined type electronic detector adopts a structure that yttrium aluminum garnet crystals are arranged to partially cover a split type silicon diode detector, so that physical replacement of any detection body is not needed, and the detection efficiency is improved. According to the invention, signals of different areas can be selected and read through an electronic mode, so that an optimal detection mode is called under different working conditions, detection can be realized under the condition of high acceleration voltage, detection can also be realized under the condition of low acceleration voltage, one machine with two purposes is realized, and the performance and the application range of electron beam equipment are greatly improved.

The invention discloses an efficient debugging preparation method of a DFB laser grating. The method comprises the following steps: obtaining the width and depth of a grating line through electron microscopic imaging to form an initial parameter set; comparing design parameters to identify an abnormal area; analyzing a spatial distribution rule of abnormal region parameters, and classifying process fluctuation types; performing selective filtering on the geometric parameter sequence according to the fluctuation type to obtain a correction parameter; evaluating the influence on the output wavelength according to the correction parameter, and generating a process compensation coefficient when the deviation is predicted; predicting and optimizing structural parameters through process simulation; and finally generating a control instruction to drive the etching equipment to adjust the process in real time, and determining that the performance reaches the standard through monitoring. According to the invention, the fluctuation source can be intelligently diagnosed, the parameters are accurately corrected, closed-loop control is realized, and the anti-interference capability, control precision and efficiency of the preparation process are effectively improved.

The utility model belongs to the technical field of medical instruments, and particularly relates to an on-site rapid pathology operation platform which comprises a machine frame, a base, universal wheels arranged at the four corners of the bottom of the base, a box body arranged at the top of the base, a workbench arranged at the top of the box body and a conveying mechanism arranged at the top of the workbench. The flaking assembly comprises a bracket arranged on one side of the top of the workbench, a coloring agent storage tank arranged on the top of the bracket and a dyeing spray head arranged at the bottom of the coloring agent storage tank, and the dyeing spray head is located over the conveying mechanism; the film reading assembly comprises a hinge frame arranged on the other side of the top of the workbench, an electron microscope arranged on the hinge frame and a carrying plate arranged on the top of the workbench, and the carrying plate is located under the electron microscope; the novel film reading device is reasonable in structure, can be used for quickly preparing and reading films on site in the use process, is beneficial to improving the diagnosis efficiency, and can reduce the working intensity of medical personnel.

A positioning system for an electron microscope, comprising: a first carriage and a second carriage, the first carriage comprising a holder for holding a workpiece. The first carriage is coupled to one or more first drive units configured to position the workpiece along a first axis, a second axis, and a third axis and along a first tilt axis. The second carriage houses the one or more first drive units and is coupled to one or more second drive units configured to position the workpiece along a second tilt axis.

The utility model discloses a temperature control loading device for a scanning electron microscope, which relates to the technical field of microscopes and comprises a sample chassis, a cooling chamber is fixedly mounted on the sample chassis, a cooling cover is mounted at the top of the cooling chamber in a threaded manner, and an objective table is fixedly mounted on the top surface of the cooling cover. A plurality of air guide holes are uniformly formed in the cooling cover, a temperature sensor is arranged on the objective table, and a temperature control assembly is arranged in the cooling cover. The temperature of the objective table is detected through the temperature sensor and is fed back to a control system of the scanning electron microscope for judgment; if the biological sample is about to exceed the cooling temperature range required by the biological sample, the face gear can be driven to rotate by the driving motor, and the threaded rod can be driven to rotate by the meshing straight gear, so that the baffle can slide along the sliding chute to gradually shield or open the air guide hole, and the cooling temperature is controlled by controlling the size of the air guide hole; the device is suitable for different biological samples.

The invention discloses a bulk electron microscopic image splicing method based on a multi-scale image pyramid, and belongs to the technical field of image processing. According to the method, two-stage local abnormal value filtering and RANSAC enhancement based on local geometric constraints are introduced in the image registration stage, and image enhancement, feature re-extraction and multi-iteration TPS displacement field calculation are adopted in local deformation correction, so that the adaptability and robustness of the algorithm to a complex biological sample microstructure and potential deformation are jointly improved. By introducing a multi-scale image pyramid structure and a hierarchical processing strategy, a complex fusion problem of a high-resolution image is decomposed into a plurality of low-resolution sub-problems, and the calculation efficiency and the memory utilization rate are remarkably improved; and the problem of fuzzy texture details caused by a traditional weighted average method is effectively avoided.

The utility model relates to the technical field of clamping, in particular to a clamping device for detecting a sample, which comprises a base and a clamping mechanism, a rectangular groove is arranged in the middle of the base, and test paper for detecting the sample is placed in the rectangular groove; the clamping mechanism comprises a clamping cover plate and a positioning block, four inserting grooves are formed in the top of the base and located in the outer side of the side length of the rectangular groove, and the positioning block is arranged at the bottom of the clamping cover plate and matched with the inserting grooves. Clamping blocks are telescopically arranged on the side walls of the positioning blocks, clamping grooves are formed in the side walls of the inserting grooves, and the clamping blocks are clamped and matched with the clamping grooves; a pressing block is arranged at the bottom of the clamping cover plate and is used for pressing corners of the test paper of the detection sample. According to the utility model, the displacement of the test paper sample in the observation process of the electron microscope due to vibration and the like can be effectively prevented, and the accuracy of the observation result is ensured.

1. Name of the product in this design: Body Fluid Detector (HCY-JCH-A). 2. Purpose of this design: To detect bodily fluids and realize the function of an electron microscope. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

A method of sample preparation in association with an electron microscope. In one embodiment, a carrier supporting a biological sample is received. A high-pressure freezing (HPF) protocol is then applied to the carrier. The protocol comprises a first phase, and a second phase that follows the first phase. The first phase comprising (i) comprising: a pressurizing operation applying a pressure to the carrier, and (ii) a rapid cooling operation using a coolant that avoids a Leidenfrost effect at a carrier-coolant interface by maintaining nucleate boiling or substantially suppressing film boiling upon contact with the carrier. The pressurizing and rapid cooling operations freeze the biological sample while preserving its structural integrity. The second phase involves raising a temperature of the carrier and the frozen biological sample to a given temperature for one of: storage, and use.

The utility model discloses a portable pulse imaging cell recognition device which comprises a bottom plate, a mounting plate is hinged to one side of the bottom plate, a placement groove is formed in the inner wall of the mounting plate, a display operation panel is hinged to the inner wall of the placement groove, and a supporting plate is hinged to the outer wall of one side of the display operation panel. Clamping grooves matched with the supporting plates in a limiting mode are formed in the inner walls of the containing grooves, a plurality of inserting grooves are formed in the outer wall of the top of the bottom plate, inserting blocks are inserted into the inner walls of the inserting grooves, hand screwing bolts matched with the inserting blocks in a limiting mode are in threaded connection with the inner walls of the inserting grooves, side plates are connected to the top ends of the inserting blocks, and convex edges are fixedly connected to the tops of the side plates and the tops of the mounting plates. The outer walls of the multiple convex edges are in sliding fit with the same sealing cover. By arranging the mounting plate, the supporting plate, the convex edge, the slot, the side plate, the insertion block and the sealing cover, the electron microscope main body, the bearing assembly and the display operation panel can be folded into a rectangular whole, so that the device is convenient to carry.

The invention discloses a high-throughput screening method for TMV (Tobacco Mosaic Virus) resistant microorganisms. The high-throughput screening method comprises the following specific steps: (1) purifying TMV; (2) activation of a to-be-detected strain: inoculating a single colony into a liquid culture medium, culturing on a constant-temperature shaking table under the conditions of 28 DEG C and 180 r / min for 24-48 hours, and performing co-incubation of the to-be-detected strain and the virus when the OD600 value is within the range of 0.6-1; (3) co-incubation: culturing the bacterial liquid to be detected and viruses on a constant-temperature shaking table for 0-3 days under the conditions of 28 DEG C and 180r / min, and taking 1mL of co-incubation liquid every 24 hours for a subsequent antiviral activity detection test; and (4) antiviral activity detection: detecting the virus concentration after co-incubation by adopting methods such as Elisa, an electron microscope and RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction). According to the invention, the screening efficiency is improved, microorganisms or compound resources with tobacco mosaic virus resisting activity are quickly found, and a new solution is provided for agricultural disease control.

This invention relates to the field of stem cell culture technology, and particularly to a stem cell culture device and its method of use, comprising a housing, a base plate, and a cover plate. An annular observation window is provided on the cover plate, and a detection mechanism for observing stem cells is also provided on the cover plate. By positioning the movement trajectory of the sample carrier, liquid level fluctuations caused by tilting of the culture flask during ascent are effectively avoided. Combined with the pressure regulation function controlled by the micro-flow one-way valve of the telescopic damping airbag, stable and uniform lifting and lowering of the sample carrier component is achieved. With the assistance of annular scanning by an electron microscope, multiple batches of samples can be tested without manual intervention. This effectively avoids the acceleration impact caused by traditional sample transfer methods, ensuring the stability of the stem cell suspension and effectively preventing the impact of mechanical vibration on stem cell growth, thereby effectively improving the accuracy and repeatability of stem cell detection data.

The invention discloses an ultralow-temperature freezing sample rod of a transmission electron microscope, and belongs to the low-temperature technical field of transmission electron microscopes. The sample rod comprises a grip, a rod body, a liquid nitrogen precooling tank and a sample bearing assembly; a heat exchange channel is integrated in the liquid nitrogen precooling tank; a coaxial heat exchanger, a shunting chamber and an expansion chamber are arranged in the rod body, a pipeline connected with a cold source and a heat exchange channel is arranged in the grip, the heat exchange channel is further connected with an inner pipe of the coaxial heat exchanger, an outer pipe of the coaxial heat exchanger is hermetically connected with the grip and the shunting chamber respectively, the inner pipe penetrates through the shunting chamber and is connected with the expansion chamber, and the shunting chamber is connected with the expansion chamber. The end of the expansion chamber is hermetically connected with the sample bearing assembly; an expansion valve is arranged at the end, connected with the expansion chamber, of the inner pipe and used for cooling and depressurizing gas entering the expansion chamber through the expansion valve. The flow dividing chamber is used for dividing the cooled and depressurized gas input through the second pipeline, so that the flow speed of the gas is uniform. The method is suitable for extremely-low-temperature in-situ research.

The application discloses a three-dimensional feature recognition method, device, equipment and storage medium. The method comprises the following steps: acquiring a first electron microscopic image; generating first frequency domain information corresponding to the first electron microscopic image; analyzing the first frequency domain information based on a three-dimensional feature recognition model to obtain feature data of a three-dimensional structure corresponding to the first electron microscopic image; and outputting the feature data of the three-dimensional structure corresponding to the first electron microscopic image. It can be seen that, by means of the three-dimensional feature recognition model, the first frequency domain information of the first electron microscopic image generated by the CD-SEM can be directly analyzed and processed, without additional hardware modification of the existing detection equipment, so that the detection efficiency of the feature data of the three-dimensional structure can be improved, and the detection cost can be reduced. Moreover, the feature data of the three-dimensional structure is captured in the frequency domain, so that the key features of the data can be more directly extracted, efficient and accurate feature recognition is realized, and the accuracy of micro-nano structure analysis is effectively improved.

The application provides a sample transfer device applied to a scanning electron microscope quick sample changing chamber, and belongs to the technical field of scanning electron microscope accessories, and aims to solve the problems of limited universality of the existing transfer device, incompatibility with the quick sample changing chamber and sample recovery difficulty. The device comprises a cylinder assembly, a limiting assembly and a pressure adjusting assembly. The cylinder assembly comprises a barrel box and a sample groove slider provided with a sample groove. Three sealing rings are arranged on the outer surface of the slider to form three sealed spaces in the box. The limiting assembly comprises a guide rod and a limiting plate, which is used for limiting the sliding-out distance of the slider, ensuring that the sample groove is completely exposed after sliding out and the second sealing ring is still located in the box. The pressure adjusting assembly comprises a locking screw and a sealing plug for adjusting the required air pressure for the sliding-out of the slider. The application is small in size, compatible with conventional sample chambers and quick sample changing chambers, can realize automatic sliding-out of the sample under vacuum and automatic sealing after testing, and is safe and reliable in operation.

The present application provides a method for protein structure analysis using cryo-EM, comprising: including a tag in a target protein; combining the target protein including the tag with a scaffold protein to form a complex of the target protein and the scaffold protein; and using a cryo-EM for single particle imaging to analyze the structure of the target protein complexed with the scaffold protein. The scaffold protein is any one of streptavidin, avidin or a derivative thereof. The tag is used to selectively bind to the scaffold protein. The tag is selected from one of the following group: a biotin tag including biotin; a biotinylated protein or polypeptide tag including a protein sequence and biotin covalently linked to the protein sequence; a streptomyces tag (Strep-tag); and a biotinylated or streptococcal labeled antibody, antibody Fab fragment or single chain antibody.

An electrophotographic roller includes: a conductive substrate; and a foamed layer on an outer circumferential surface of the substrate, in which the foamed layer is a surface layer of the electrophotographic roller, the foamed layer has a skeleton including a binder resin, and at least one conductive particle in the binder resin, the foamed layer has a communication structure having at least one gap opening, and extending in a thickness direction of the foamed layer, at least a portion of an inner wall of the gap includes the skeleton, at least a portion of the at least one conductive particle is exposed at the inner wall, a thickness of the foamed layer is 2.0 mm or more, and in the observation of the cross section of the foamed layer by a scanning electron microscope, the ratio of number of pixels of a specific brightness falls within a specific range.

This invention belongs to the field of metallic material preparation technology, and specifically relates to a machine learning-based design method for interstitial element-strengthened titanium alloys in additive manufacturing. This machine learning-based design method includes: constructing a physical information-enhanced feature space; establishing an original dataset; augmenting the original dataset using data augmentation methods to establish an augmented dataset; using a multi-step feature screening strategy to determine key features affecting material properties; establishing a key feature-material property prediction model; using a multi-objective optimization strategy to collaboratively optimize multi-objective properties; and performing additive manufacturing to obtain a part; and using transmission electron microscopy to characterize the microstructure of the part. The design method provided by this invention can effectively solve the problems of high cost, long cycle, and low reliability in the composition and process optimization design of high-performance titanium alloys.

The invention relates to the field of precise instruments, in particular to a lifting sample loading device and a scanning electron microscope. The lifting sample loading device is used for the scanning electron microscope and comprises a base, a driving mechanism and a sample loading table, the scanning electron microscope comprises a sample table, and the base is detachably connected with the sample table; the driving mechanism is installed on the base, and the driving end of the driving mechanism is connected with the sample carrying table so as to drive the sample carrying table to ascend and descend. According to the lifting sample loading device provided by the invention, the driving mechanism is arranged to drive the sample loading table to perform vertical lifting motion so as to adjust the height of the sample, and the height of the sample does not need to be adjusted by changing the sample, so that the sample changing operation time can be shortened, and the working efficiency of a user is improved. A user can flexibly mount or remove the lifting sample loading device according to different experiment requirements, and basic functions of original equipment are not affected.

This invention discloses an intelligent identification method and system for fracture morphology features of metallic materials, belonging to the field of failure analysis and intelligent image recognition technology of metallic materials. The method performs multi-scale feature extraction on fracture morphology images acquired by scanning electron microscope, identifies eight types of micro-features such as dimples, cleavage surfaces, and fatigue striations, determines five types of fractures such as ductile fracture, brittle fracture, and fatigue fracture through a multi-label classification network, calculates quantitative parameters such as dimple size distribution and fatigue striation spacing, and establishes a correlation model between fracture features and fracture mechanical parameters.

Embodiments of the present application disclose a sample state detection method applied to biological samples and a computer device. A specific embodiment of the method comprises: determining state detection configuration information for a target biological sample group; moving the target biological sample group to a sample storage device according to the storage configuration information to store the target biological sample; in response to reaching a sample detection time point, performing the following processing steps for the target biological sample: sucking the target biological sample to a slide making device by a pipetting device to generate a slide to be detected; controlling an electron microscope to collect electron microscope images of the slide to be detected at different image scales to obtain an electron microscope image group; generating sub-sample detection information for the target biological sample; and generating sample detection information for the target biological sample group according to the obtained sub-sample detection information group. The embodiment guarantees consistency of sample detection and greatly improves sample detection efficiency.

The invention provides application of a GSDMD inhibitor in preparation of a chronic obstructive pulmonary disease (COPD) treatment preparation, and relates to the technical field of biological medicines.The COPD mouse model induced by cigarette smoke is established and divided into a control group, a COPD group and a COPD + inhibitor group (GS DMD inhibitor), and the GS DMD inhibitor and the COPD + inhibitor group (GS DMD inhibitor) are combined through methods of Western Blot, ELISA, qPCR, a transmission electron microscope and the like, so that the chronic obstructive pulmonary disease (COPD) treatment preparation is obtained. And detecting GSDMD pathway related protein and gene expression, inflammatory factor release level and alveolar barrier function change. A GSDMD channel in the COPD model group is obviously activated, which shows that the expression levels of GSDMD-N and Caspase-1 are increased; the concentration of IL-1beta and IL-18 is obviously increased; the expression levels of NLRP3, ASC and pro-Caspase-1 and the concentration of HMGB1 in BAL F are increased, the alveolar epithelial cell structure is damaged, and the wet-to-dry ratio and the total protein content are increased. The GSDMD inhibitor significantly reduces the above indexes and improves the airway epithelial barrier function. It is determined that the GSDMD pathway aggravates the airway epithelial cell injury and inflammatory response of COPD by inducing pyroptosis. The GSDMD inhibitor can significantly inhibit pyroptosis and improve COPD-related pathological changes, and provides a new target and theoretical basis for treatment of COPD.

A first grid box assembly for holding a plurality of sample-bearing grids for use in cryo-electron microscopy comprises: a grid box comprising a plurality of compartments, each compartment adapted to hold a respective sample-bearing grid on a sample carrier; and a sample grid stabilization apparatus comprising a plurality of spring arms, wherein each spring arm provides a spring force to a respective sample carrier that secures the position of the sample carrier within its respective compartment. A second grid box assembly for holding a plurality of sample-bearing grids for cryo-electron microscopy comprises: a grid box comprising a plurality of compartments, each compartment adapted to hold a respective sample-bearing grid on a sample carrier; and a lid adapted to cover a top surface of the grid box, wherein a bottom surface of the grid box comprises a plurality of gas dispersion pockets the extend to the perimeter of the grid box.

Systems and methods for transferring a sample from a transmission electron microscope (TEM) column are described herein. In one aspect, a method can include transferring a sample positioned in a holder capsule of a TEM sample holder from a vacuum chamber of a TEM into a load lock of the TEM; filling the load lock with a protecting gas; sealing the holder capsule of the TEM sample holder under the protecting gas; and removing the TEM sample holder from the load lock.

A device for preparing samples for use in a cryo-electron microscope. The device comprises a holder for holding a sample carrier, at least one jetting device that is arranged for providing a jet of cryogenic fluid to said sample carrier, and a transfer mechanism that is operably coupled to the holder for bringing the holder to a jetting position. In the jetting position the holder is located near the at least one jetting device for receiving said jet of cryogenic fluid. The device further comprises a plunging reservoir for receiving a volume of cryogenic fluid, wherein the transfer mechanism is additionally arranged for bringing the holder to a plunging position. In the plunging position the holder is positioned at least partly inside an internal volume of said plunging reservoir for bringing the sample carrier in contact with cryogenic fluid.

The application relates to the technical field of electron microscopic imaging, and provides an objective lens and an environmental scanning electron microscope. The objective lens comprises at least: an upper pole shoe having a connecting portion and a working portion, the working portion is provided with a hollow accommodating cavity, and the working portion is suitable for forming an electron beam channel; a lower pole shoe connected with the connecting portion, and the working portion is at least partially inserted into the lower pole shoe, a region between an inner side wall of the lower pole shoe and an outer side wall of the working portion forms a transition vacuum area; a first air exhaust hole is arranged on a side wall of the lower pole shoe, and the transition vacuum area is suitable for being vacuumized through the first air exhaust hole; a sealing structure is arranged at one end of the lower pole shoe away from the upper pole shoe, so as to separate the electron beam channel from the transition vacuum area, and separate the transition vacuum area from an external environment; the sealing structure is provided with a gas channel and a first through hole through which an electron beam passes, and the gas channel is communicated with the first through hole and the transition vacuum area. The objective lens is more convenient to maintain high vacuum degree of the electron beam channel in a scanning process, so that the scanning effect is improved.

Protein double-shell nanostructures comprising apoferritin for carrying cargo proteins of interest are provided. Such nanostructures can be used to increase rigidity of a cargo protein of interest to allow structures of small and flexible proteins to be determined by cryogenic-electron microscopy (cryo-EM). Recombinant vectors for producing protein double-shell nanostructures are also provided. The nanostructures described herein may find use in various applications in research and drug discovery.

The invention relates to the technical field of biological medicine, and particularly provides a medicine for relieving cell damage caused by heavy ion radiation and application of the medicine. The medicine can obviously improve the cell cloning survival rate under the condition of carbon ion radiation, inhibit necroptosis and weaken inflammation-related transcription reaction caused by radiation. According to the present invention, the multi-level experiments such as clone formation experiment, protein level detection, transmission electron microscope observation, transcriptomics analysis and the like prove that the pharmaceutical composition can specifically improve the cell survival state after carbon ion irradiation, and has no significant protection effect on X-ray irradiation; the invention provides a new pharmacological scheme for heavy ion radiotherapy related protection and complication intervention, and has important application prospects.

The invention relates to a full-automatic constant-force low-speed liquid nitrogen cutting machine, which relates to the field of electron microscopes, and comprises a lifting structure, a liquid nitrogen pool arranged on the lifting structure, a saw blade and a sample clamping mechanism arranged above the liquid nitrogen pool, and a protective shell movably arranged above the saw blade and the sample clamping mechanism, a sliding rail and a second lead screw which are arranged in the width direction of the cutting machine are arranged on the side, away from the saw blade, of the sample clamping mechanism, the differential sliding table is movably arranged on the sliding rail, the second lead screw penetrates through the differential sliding table, and the driving motor and the manual rocker are arranged at the two ends of the second lead screw correspondingly and used for driving the second lead screw to rotate. The sample clamping mechanism is arranged at the bottom of the end, away from the differential sliding table, of the transverse plate. Position sensors are arranged at the two ends of the sliding rail. The force sensor is arranged on the transverse plate, monitors cutting force in real time through the strain gauge and achieves constant-force closed-loop control. The problems that existing liquid nitrogen cutting equipment cannot guarantee low-temperature environment stability, lacks a constant force control function and is large in liquid nitrogen consumption are solved.