155 results about "Electron microscopic" patented technology

The invention discloses a preparation method and application of a tension sensor for monitoring cortisol in sweat. According to the scheme, on the basis of a stretchable electrode (SWNTs / PDMS) which is prepared by patterning in a manner that a single-walled carbon nanotube is subjected to suction filtration and transfer printing on polydimethylsiloxane, cortisol is used as an imprinting molecular template by utilizing an electrochemical deposition technology, and Prussian blue (PB) and pyrrole (Py) are electrically polymerized to prepare an electrochemical sensor of a molecularly imprinted polymer (MIP), so that the change of the concentration of cortisol in sweat can be monitored in real time; a reliable and practical method is provided for rapid and pressure-free pressure monitoring. A scanning electron microscope is used for electrode modification morphology characterization, and an electrochemical workstation is used for electrode sensing performance characterization. The sensor realizes the function of dynamically monitoring the day and night change rule of cortisol in sweat, and has the characteristics of real-time detection, high sensitivity and selectivity and good stability.

The embodiment of the invention discloses a sample state detection method applied to a biological sample and computer equipment. A specific embodiment of the method comprises the following steps: determining state detection configuration information for a target biological sample group; according to the storage configuration information, moving the target biological sample group into a sample storage device so as to store the target biological samples; in response to the arrival of the sample detection time point, executing the following processing steps on the target biological sample: sucking the target biological sample to a slide manufacturing device through a pipetting device to generate a to-be-detected slide; controlling the electron microscope to collect electron microscope images of the to-be-detected slide under different image scales to obtain an electron microscope image group; generating sub-sample detection information for the target biological sample; and generating sample detection information for the target biological sample group according to the obtained sub-sample detection information group. According to the embodiment, the consistency of sample detection is ensured, and meanwhile, the sample detection efficiency is greatly improved.

The invention relates to a paper substrate bio-based high-barrier Pickering emulsion coating and a preparation method and application thereof, and belongs to the technical field of paper coatings, the prepared coating has excellent waterproof and oil-proof performance and is suitable for surface coating of substrates such as paper, the emulsion is prepared by taking glycerol as a plasticizer and chitosan as an oil-proof agent, and the emulsion is prepared by taking the chitosan as an oil-proof agent. The cellulose nanocrystal is used as an emulsifier, and the alkyl ketene dimer AKD and palm wax are used as an oil phase. After the emulsion is coated on the surface of paper, a high-barrier coating with a core-shell structure is formed, and the core-shell structure is clear through electron microscope observation; the hydrophobic angle of the coating can reach 130 degrees to the maximum, and the coating shows remarkable hydrophobicity and oil resistance. The coating is composed of bio-based materials, has the advantages of environmental protection and sustainability, is suitable for application requirements of degradable and green packaging, is simple in preparation process and excellent in performance, and can provide efficient barrier performance for paper packaging materials.

The invention provides a virus particle identification method based on a negative staining electron microscope image. The deep learning and image segmentation technology is adopted to automatically identify virus particles and classify hollow and solid virus particles in the negative staining electron microscope image, so that the real heart rate and the virus particle concentration of the virus are obtained, and the requirement on the purity of a sample is low. Therefore, the production quality control process of the virus vector can be improved, and the development and optimization of gene therapy can be accelerated.

The invention belongs to the field of application of artificial intelligence in physical and chemical research, and provides a catalytic metal particle electron microscope image recognition method based on density estimation, which comprises the following steps: sending an electron microscope image into a backbone network for feature extraction to generate feature maps with different sampling multiplying power; inputting the feature maps with different scales into an expansion feature pyramid network, and carrying out multi-level feature fusion; sending the data to a self-attention expansion pyramid pooling module, and performing up-sampling to obtain a predicted density map; performing reverse distance transformation on the input original image to obtain a density map true value; using the predicted density map and the true value density map to calculate strong and weak loss, and weighting the two to jointly supervise the training process; performing local maximum positioning on the density map predicted by the final model through a prediction density map target positioning algorithm to obtain a target position coordinate; and finally, calculating to obtain a density and distance distribution curve of the monatomic, and marking the monatomic target in an original image. According to the invention, high-precision identification of the electron microscope image of the catalytic metal particles can be realized.

The present invention relates to the technical field of diabetes, and in particular to a controlled-release diabetic wound healing promoting treatment method, comprising the following treatment experimental steps: step S1: preparation of Hst1@PNPs; step S2: Coomassie brilliant blue staining; step S3: scanning and transmission electron microscopy detection; in the present invention, a slow-release Hst1 treatment method is constructed by encapsulating Hst1 into platelet membrane vesicles derived from autologous blood. On the one hand, the PNPs encapsulate the linear Hst1 peptide structure to prevent it from being rapidly degraded by high proteases in diabetic wound exudate, thereby improving bioavailability. On the other hand, the PNPs can adsorb excess LPS in the wound exudate, and cooperate with Hst1 to achieve anti-inflammatory and anti-oxidative stress effects. Subsequent cell and animal experiments further confirmed that Hst1@PNPs have the effects of anti-inflammatory, scavenging ROS, promoting re-epithelialization, collagen deposition and angiogenesis, thereby promoting diabetic wound healing.

The invention discloses a novel nano-drug as well as a preparation method and application thereof, and co-delivery of siRNA and 3BP is realized on an exosome nano-platform, so that a novel nano-drug Exo (at) siRNA / 3BP-CTX (ERBC) engineering exosome is generated. The surface-modified cetuximab (CTX) is targeted to tumors, and shows high targeting and low toxicity in imaging analysis. CCK-8, western blot and immunofluorescence detection are used for detecting the tumor killing effect and the synergistic effect. The ERBC finds that the occurrence of death-promoting autophagy in KRAS mutation tumors is promoted on the transmission electron microscope (TEM) and molecular level.

The present disclosure provides to electron microscopy grids and methods, kits, and systems for using and fabricating thereof. More particularly, the present disclosure provides electron microscopy grids comprising a single first layer comprising graphene, wherein the graphene covers greater than 90% of the grid, and a second layer comprising a binding agent configured to interact with a target protein.

The present invention discloses a method for batch preparation of graphene electron microscope grids. The present invention realizes high-quality batch transfer of graphene and electron microscope grids by designing a multi-level transfer medium (including a PDMS porous template, a polymer support layer and a small molecule buffer layer) in combination with a solvent volatilization-induced elastic capillary bonding process. Specifically, the surface of the graphene is uniformly coated with a small molecule / polymer transfer medium layer, and then bonded to the PDMS porous array template. After the graphene is peeled off from the growth substrate, the graphene supported by the polymer film in the suspended part of the PDMS is bonded to the electron microscope grid using a solvent thermal bonding method; finally, the transfer medium is removed to obtain the graphene grid. The method of the present invention has the advantages of simple process, reusable PDMS template and good compatibility, and is suitable for large-scale production. At the same time, the prepared graphene grid has high cleanliness and integrity, and can meet the application requirements in the field of cryo-electron microscope grids.

Methods and systems for calibrating a transmission electron microscope are disclosed. A fiducial mark on the sample holder is used to identify known reference points so that a current collection area and a through-hole on the sample holder can be located. A plurality of beam current and beam area measurements are taken, and calibration tables are extrapolated from the measurements for a full range of microscope parameters. The calibration tables are then used to determine electron dose of a sample during an experiment at a given configuration.

The invention provides a combined type electronic detector, electronic detection equipment and a detection method, and belongs to the technical field of electronic microscopic imaging. The combined type electronic detector adopts a structure that yttrium aluminum garnet crystals are arranged to partially cover a split type silicon diode detector, so that physical replacement of any detection body is not needed, and the detection efficiency is improved. According to the invention, signals of different areas can be selected and read through an electronic mode, so that an optimal detection mode is called under different working conditions, detection can be realized under the condition of high acceleration voltage, detection can also be realized under the condition of low acceleration voltage, one machine with two purposes is realized, and the performance and the application range of electron beam equipment are greatly improved.

The invention relates to a low-stress chemical mechanical etching process suitable for fragile materials, which belongs to the technical field of etching processing of fragile materials and comprises a precise chemical mechanical polishing machine, a polishing pad, a polishing solution conveying and managing system and end point detection equipment. The precise chemical mechanical polishing machine comprises a multi-zone pressure controllable polishing head, a high-precision rotating speed control system and a real-time thickness measuring system, a polishing pad is a polishing disc made of porous polyurethane materials, and the polishing liquid conveying and managing system comprises a multi-channel metering pump system and a stirring and temperature control unit. And the end point detection equipment comprises a surface profilometer, an atomic force microscope and a transmission electron microscope. According to the method, through fine cooperation of chemical reaction and mechanical grinding, microcrack initiation and propagation in the polishing process are effectively inhibited, surface and subsurface damage of the fragile material is remarkably reduced, the machined surface which is ultrahigh in smoothness and free of damage is obtained, and meanwhile the high material removal rate is kept.

The invention discloses an efficient debugging preparation method of a DFB laser grating. The method comprises the following steps: obtaining the width and depth of a grating line through electron microscopic imaging to form an initial parameter set; comparing design parameters to identify an abnormal area; analyzing a spatial distribution rule of abnormal region parameters, and classifying process fluctuation types; performing selective filtering on the geometric parameter sequence according to the fluctuation type to obtain a correction parameter; evaluating the influence on the output wavelength according to the correction parameter, and generating a process compensation coefficient when the deviation is predicted; predicting and optimizing structural parameters through process simulation; and finally generating a control instruction to drive the etching equipment to adjust the process in real time, and determining that the performance reaches the standard through monitoring. According to the invention, the fluctuation source can be intelligently diagnosed, the parameters are accurately corrected, closed-loop control is realized, and the anti-interference capability, control precision and efficiency of the preparation process are effectively improved.

The invention provides an extracellular vesicle marker separation-free detection method based on nanoparticle enhancement, and belongs to the technical field of detection methods, the method comprises the following steps: preparing gold nanoparticles with the surface modified with a first antibody, the first antibody being used for specific recognition of an extracellular vesicle surface marker; determining the particle size distribution of the gold nanoparticles by adopting a dynamic light scattering method, and determining the surface potentials and surface isoelectric points of the gold nanoparticles by adopting a potentiostat; adopting a transmission electron microscope to characterize the morphology of the screened gold nanoparticles; co-modifying polyethylene glycol molecules on the surfaces of the gold nanoparticles to form an anti-interference layer; mixing the gold nanoparticles with uniform morphology with a sample to be detected; adding a second antibody into the mixed system; distinguishing signals of the binding-state second antibody and the free-state second antibody through fluorescence lifetime analysis; preparing an extracellular vesicle standard substance, and obtaining a standard curve and a system response curve; the optimal detection condition is determined by solving the detection system optimization equation set, and separation-free detection is carried out.

The invention discloses an intelligent analysis system and method for an electron microscopic image crystal structure, and the system comprises a data importing module which is used for importing an electron microscopic image and crystal structure data; the image preprocessing module is used for preprocessing the imported electron microscopic image to obtain an experimental map; the diffraction simulation module is used for simulating two-dimensional electron diffraction of the crystal based on the imported crystal structure data to obtain a diffraction simulation result, and processing the diffraction simulation result to obtain a theoretical map; the intelligent matching module is used for matching the experimental map with the theoretical map and outputting a matching result; and the result output module is used for outputting the matching result and the diffraction simulation result to realize the visualization of the crystal structure. Under the synergistic effect of all the modules, the crystal structure of the electron microscopic image can be automatically analyzed in the whole process, and the analysis efficiency of the crystal structure is greatly improved; and meanwhile, under the action of the intelligent matching module, rapid matching can be realized, and the misjudgment rate is reduced.

The invention discloses a FeAl nanoparticle-loaded catalytic material as well as a preparation method and application thereof, can be used as an electro-catalysis nitrogen reduction reaction catalyst, and belongs to the field of nano material synthesis and energy environment science and engineering application. Dispersed activated carbon is used as a conductive substrate and is loaded with FeAl nano active particles, and the dispersed activated carbon and the FeAl nano active particles are synergistically used for an electro-catalysis nitrogen fixation reaction. FeAl metal particles loaded in the carbon black can be clearly seen in a transmission electron microscopic image, and when an electro-catalysis nitrogen reduction reaction test is carried out, the ammonia production selectivity reaches 26.6% at most.

The invention belongs to the technical field of epoxy resin and impregnated paper sample wafer aging defect diagnosis, and discloses an epoxy resin and impregnated paper sample wafer aging defect diagnosis and service life prediction system. Comprising a thermal aging treatment module, a dielectric property test module, a main control module, a mass loss calculation module, an aging degree analysis module, a physicochemical property detection module and a life prediction module. According to the invention, a thermal aging test platform for the epoxy resin sample wafer and the epoxy impregnated paper sample wafer is established, the research on the aging degree rule of the sample wafer is completed from multiple angles of dielectric properties, Fourier infrared spectroscopy, mass loss rate and scanning electron microscope, and the research on the aging degree rule of the epoxy resin sample wafer and the epoxy impregnated paper sample wafer along with the deepening of the aging degree is elaborated. The changes of the surface appearance and the micromolecular structure of the dry-type bushing are explained in combination with the dielectric property, and a theoretical basis is provided for subsequent dry-type bushing aging degree judgment.

The invention discloses a flexible particle deformation detection method and system based on deep learning. The method and system are suitable for classification of two-dimensional particles in a Cryo-EM image. For the problems of low signal-to-noise ratio, complex particle deformation and the like in a Cryo-EM image, noise reduction processing is performed on an original image through methods of Topaz, low-pass filtering, non-local mean filtering and the like, so that the structural distinguishability is improved; then, carrying out binarization and connected region detection to obtain a particle contour, and generating a corresponding two-dimensional point cloud expression; in order to realize efficient registration, a KMP heuristic point cloud matching algorithm is provided, and the corresponding relation between the particle point cloud and the template point cloud is effectively established; on the basis of matching, continuous deformation tracks of the simulated particles are predicted through multi-wheel displacement, detection of deformation values is achieved, and the deformed particles are removed to achieve the classification effect. According to the method, the precision and robustness of flexible structure modeling are improved while the point cloud matching efficiency is improved, and the method is suitable for a particle structure analysis task under a high-noise condition.

The utility model belongs to the technical field of medical instruments, and particularly relates to an on-site rapid pathology operation platform which comprises a machine frame, a base, universal wheels arranged at the four corners of the bottom of the base, a box body arranged at the top of the base, a workbench arranged at the top of the box body and a conveying mechanism arranged at the top of the workbench. The flaking assembly comprises a bracket arranged on one side of the top of the workbench, a coloring agent storage tank arranged on the top of the bracket and a dyeing spray head arranged at the bottom of the coloring agent storage tank, and the dyeing spray head is located over the conveying mechanism; the film reading assembly comprises a hinge frame arranged on the other side of the top of the workbench, an electron microscope arranged on the hinge frame and a carrying plate arranged on the top of the workbench, and the carrying plate is located under the electron microscope; the novel film reading device is reasonable in structure, can be used for quickly preparing and reading films on site in the use process, is beneficial to improving the diagnosis efficiency, and can reduce the working intensity of medical personnel.

The present invention discloses a sample sealing and transfer device, which includes a housing. A window is provided on the housing. A lifting mechanism is arranged inside the housing. A sample support platform is connected to the moving end of the lifting mechanism. A sample accommodation groove is formed in the sample support platform. The sample support platform is lifted or lowered at the window by the drive of the lifting mechanism. A switchable hatch is also provided at the window, and a linkage is formed between the hatch and the lifting mechanism through a linkage component. When the hatch at the window is opened, under the linkage action of the linkage component, the lifting mechanism can automatically drive the sample support platform to rise from the window. When the hatch is closed, under the linkage action of the linkage component, the lifting mechanism can automatically drive the sample support platform to descend into the housing and push the sample support platform against the inner side of the hatch, so as to seal the sample accommodation groove. The sample sealing and transfer device of the present invention can realize sample preparation in an environmental sample preparation chamber while maintaining the environmental atmosphere and safely transfer the sample to be observed under a scanning electron microscope.

A positioning system for an electron microscope, comprising: a first carriage and a second carriage, the first carriage comprising a holder for holding a workpiece. The first carriage is coupled to one or more first drive units configured to position the workpiece along a first axis, a second axis, and a third axis and along a first tilt axis. The second carriage houses the one or more first drive units and is coupled to one or more second drive units configured to position the workpiece along a second tilt axis.

To provide titanium oxide particles with few coarse particles and excellent homogeneity and dispersibility, a method for their production, and a slurry, a dispersion, a composition and a dielectric starting material that comprise the titanium oxide particles.SOLUTION: The titanium oxide particles are such that D90 (LD) / D50 (LD) of the titanium oxide particles as measured by laser diffraction / scattering analysis is greater than 1.0 and less than or equal to 2.0, and the concentration of coarse particles whose primary particle size exceeds 16 times D50 (SEM) of primary particles as observed under a field emission scanning electron microscope is 20 ppm or less (based on the number of particles). For their production, a source gas and oxidizing gas are introduced into a reaction tube and reacted, where a blowing outlet of a purge medium is provided on the inner wall of the reaction tube, and where the blowout angle of the purge medium and the blowout flow velocity (B) of the purge medium from the inner wall of the reaction tube are introduced such that the purge medium swirls along the inner wall of the reaction tube.SELECTED DRAWING: Figure 1

The utility model discloses a temperature control loading device for a scanning electron microscope, which relates to the technical field of microscopes and comprises a sample chassis, a cooling chamber is fixedly mounted on the sample chassis, a cooling cover is mounted at the top of the cooling chamber in a threaded manner, and an objective table is fixedly mounted on the top surface of the cooling cover. A plurality of air guide holes are uniformly formed in the cooling cover, a temperature sensor is arranged on the objective table, and a temperature control assembly is arranged in the cooling cover. The temperature of the objective table is detected through the temperature sensor and is fed back to a control system of the scanning electron microscope for judgment; if the biological sample is about to exceed the cooling temperature range required by the biological sample, the face gear can be driven to rotate by the driving motor, and the threaded rod can be driven to rotate by the meshing straight gear, so that the baffle can slide along the sliding chute to gradually shield or open the air guide hole, and the cooling temperature is controlled by controlling the size of the air guide hole; the device is suitable for different biological samples.

Provided is an endoscopic surgery system with automatic hydraulic monitoring function, comprising a control system, a cart kit and an endoscope accessory. Then control system includes a processor, a touch display, a perfusion pump, a negative pressure pump, etc. The cart kit includes a cart, a perfusion tube and an extraction tube. The endoscope accessory includes a pressure measuring guide sheath, a hydraulic sensor and an electron microscope. When the endoscope is employed for observation, the invention can control the perfusion and monitoring of the liquid in endoscope instrument, as well as the cavity liquid pressure in organs, allowing for precise control of the intracavity pressure during operation. The real-time monitoring module measures the liquid pressure in the organ in real time and sends data to the control system, calculates a state of the intracavity pressure based on real-time data obtained from the hydraulic sensor using intelligent PID control.

The current invention relates to an improved microfluidic device for protein / protein complex purification, as well as an associated methodology and system.In particular it relates to systems and methods for electron microscopy, preferably cryogenic electron microscopy (cryo-EM), on protein samples, comprising: a. at least one microfluidic chip with inlet and outlet and purification device; b. at least one illumination means and detection means for, preferably fluorescence, measurements on the microfluidic chip; c. a pumping system adapted for operable connection to the microfluidic chip, and configured for controlling flow in the operably connected microfluidic chip; d. a microscopy grid, preferably a cryogenic electron microscopy (cryo-EM) grid, for holding fluid samples; e. preferably a cryogenic container, for a cryogenic coolant; f. preferably a transport system for moving the cryo-EM grid between a position for receiving a fluid sample from the microfluidic chip and the cryogenic container; g. a control system, preferably a processor, which receives information on the measurements from the detection means, configured for controlling the pumping system at least based on said information, and further configured for controlling the illumination and detection means and preferably also for controlling the transport system.

The invention belongs to the technical field of medical examination instruments, particularly relates to an optical coherence elastic imaging device combined with an ophthalmologic operation microscope, and aims to overcome the defects that the used microscope cannot be adjusted and is difficult to match with eyes of a patient when glasses of the patient are examined in the prior art. Four limiting frames are symmetrically and fixedly installed at the top of the base, sliding holes are formed in the inner walls of one sides of the limiting frames, and moving rods are slidably connected into the limiting frames. The eye examination device is simple in structure and convenient to use, can be matched with the habitual position of a patient, and can shoot the eyes of the patient through the two arranged electron microscope tubes when the eyes of the patient are examined, so that the eyes of the patient can be conveniently examined.

The invention discloses a bulk electron microscopic image splicing method based on a multi-scale image pyramid, and belongs to the technical field of image processing. According to the method, two-stage local abnormal value filtering and RANSAC enhancement based on local geometric constraints are introduced in the image registration stage, and image enhancement, feature re-extraction and multi-iteration TPS displacement field calculation are adopted in local deformation correction, so that the adaptability and robustness of the algorithm to a complex biological sample microstructure and potential deformation are jointly improved. By introducing a multi-scale image pyramid structure and a hierarchical processing strategy, a complex fusion problem of a high-resolution image is decomposed into a plurality of low-resolution sub-problems, and the calculation efficiency and the memory utilization rate are remarkably improved; and the problem of fuzzy texture details caused by a traditional weighted average method is effectively avoided.

The invention discloses a liquid sample transmission electron microscopic observation method based on a nanometer quartz glass tube. The method comprises the following steps: preparing a nano quartz glass tube by a laser program control drawing method, injecting a liquid sample into the nano quartz glass tube, cutting the tip part of the nano quartz glass tube in the presence of a sealing reagent, transferring and loading the tip part on a universal carrying net coated with a carbon film by using a capillary tube in an auxiliary manner, and then transmission electron microscopic observation can be carried out. Compared with the prior art, the method has the advantages of low cost, simple preparation process and the like, has a good resolution ratio and fluid mixing function, and has a wide application prospect in the field of transmission electron microimaging of liquid samples.

The utility model relates to the technical field of clamping, in particular to a clamping device for detecting a sample, which comprises a base and a clamping mechanism, a rectangular groove is arranged in the middle of the base, and test paper for detecting the sample is placed in the rectangular groove; the clamping mechanism comprises a clamping cover plate and a positioning block, four inserting grooves are formed in the top of the base and located in the outer side of the side length of the rectangular groove, and the positioning block is arranged at the bottom of the clamping cover plate and matched with the inserting grooves. Clamping blocks are telescopically arranged on the side walls of the positioning blocks, clamping grooves are formed in the side walls of the inserting grooves, and the clamping blocks are clamped and matched with the clamping grooves; a pressing block is arranged at the bottom of the clamping cover plate and is used for pressing corners of the test paper of the detection sample. According to the utility model, the displacement of the test paper sample in the observation process of the electron microscope due to vibration and the like can be effectively prevented, and the accuracy of the observation result is ensured.

This application relates to the field of semiconductor measurement technologies, and particularly to an electron microscopy imaging measurement method, apparatus, device, medium, and program product. The method includes: obtaining a detection image of a target object to be processed; processing the detection image based on a pre-constructed image conversion model to obtain a template image matching the detection image; determining target information of a target region in the template image based on a preset image analysis algorithm, where the target region includes a measurement target and the edge of the measurement target; generating a measurement target box corresponding to the target region according to the target information, applying the measurement target box to the detection image to determine a local region containing the target object in the detection image, and imaging the local region to achieve measurement of the target object. Using this method can improve the overall efficiency and accuracy of the measurement process.