Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof

A technology for mononucleosis and Listeria, applied in the direction of bacteria, application, and microbial-based methods, can solve the problems of bacteriophage sterilization with large limitations, damage to food nutrients, color and aroma, etc., to achieve food safety Problems, high elimination rate, health-protecting effect

Inactive Publication Date: 2010-02-17
SOUTH CHINA UNIV OF TECH
View PDF1 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary sterilization methods often cause bacterial residues. At the same time, food treated by traditional sterilization methods often destroys its original color, fragrance, and nutritional content of the food.
The new type of phage sterilization is also limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof
  • Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof
  • Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] (1) Obtain Bdellovibrio BDSM08 by ultraviolet mutagenesis method

[0068] The starting strain is Bdellovibrio BDS01. Bdellovibrio BDS01 was isolated from mud samples and was preserved in the Chinese Type Culture Collection Center of Wuhan University in Wuhan, China on August 7, 2009. The preservation number is CCTCC NO: M 209169 ; After negative staining, observe its morphology under an electron microscope: Bdellovibrio BDS01 is a single cell, arc-shaped, with a size of 0.9×0.25 μm, terminal flagella, and a length of 4 μm; After four days of cultivation, transparent circular plaques with a diameter of 1-2 mm can be formed.

[0069] According to the application number "2008100220488.3" of Bdellovibrio BDS01, the method in the national invention patent application titled "Ultraviolet-induced mutation in enhancing the lysis performance of Bdellovibrio" was used, and the power of the ultraviolet lamp was 15W, and the power of the auxiliary infrared light source was Under t...

Embodiment 2

[0090] (1) Use of microbial preparations to eliminate Listeria monocytogenes carried by aquatic products before consumption

[0091] The test container is a 25L aquarium, which is divided into a test group and a control group, and each group has 3 parallels. The test water is fresh water for cultivation filtered by filter membrane, and 16L of filtered water is injected into each aquarium. The aquarium was sterilized by sodium dichloroisocyanurate before the experiment, and the water temperature was 26 °C. Add embodiment 1 step (3) 1. to prepare Listeria monocytogenes liquid in each aquarium, adjust its final concentration with sterile water to be 1.0 * 10 4 cfu / mL. Then put 10 tilapias and 10 prawns in each aquarium. The tilapia used in the test weighs about 140-190g, the prawn weighs about 15-30g, and the body length is about 8-10cm. The matched group is to only add the Listeria monocytogenes prepared in embodiment 1 step (3) 1., adjust final concentration with sterile wa...

Embodiment 3

[0095] Microbiological agents are used to eliminate L. monocytogenes carried by eggs as follows:

[0096] The materials used in the experiment were eggs, and the experiments were set up as a control group and an experimental group. Three parallel samples are respectively set up, and each parallel sample has 10 eggs, and each egg weighs about 50g. Eggs are soaked respectively in embodiment 1 step (3) 1. the concentration prepared is 10 9 In the cfu / mL Listeria monocytogenes bacteria liquid, adjust the final concentration with sterile water to make the concentration reach 1.0×10 4 cfu / mL, remove after 30 seconds and drain. Then every 10 eggs are divided into a group, immersed in the microorganism preparation A, B, C, D, E and F that embodiment 1 prepares. The eggs were soaked for 30 seconds, taken out and drained, and placed at room temperature. The content of Listeria monocytogenes was detected at the two moments of the standing time of 2 hours and 4 hours respectively. The...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a bdellovibrio bacteriovorus bacterial strain for eliminating listeria monocytogenes and an application thereof. The invention obtains bdellovibrio bacteriovorus BDSM08 by separation, purification and ultraviolet mutation. BDSM08 is in the arc-shaped unicellular form when observed by an electron microscope, the size thereof is 1.8*1.0 mu m, the ends thereof are provided with flagellum and the length of the flagellum is 3.2 mu m. The bdellovibrio bacteriovorus BDSM08 is cultivated with the two layer plating method at the temperature of 28 DEG C for three days to form transparent round negativecolony the diameter of which is 2-3mm and the optimum growing pH value of which is 7.2, the optimum growing temperature of which is 28 DEG C and the optimum salinity of which is0%. The microbial agent prepared by BDSM08 has a strong effect on killing listeria monocytogenes which can be commonly seen in environment. The invention applies the bdellovibrio bacteriovorus BDSM08for the first time to prevent and cure food listerellosis; the invention has a favourable effect on eliminating listeria monocytogenes on food, has characteristics of no toxicity, no side effect on food and the like, and has favourable application prospect.

Description

technical field [0001] The invention relates to a Bdellovibrio, in particular to a Bdellovibrio strain for eliminating Listeria monocytogenes and application thereof. Background technique [0002] Listeria monocytogenes (Listeria monocytogenes), referred to as Listeria monocytogenes LM, is a zoonotic pathogen. It exists widely in nature and exists in most foods such as vegetables, milk and dairy products, raw meat, cooked meat products and aquatic products. Listeriosis can result from eating food contaminated with Listeria monocytogenes. Listeriosis is associated with a high mortality rate and is second only to salmonella infection as the most deadly foodborne disease. According to the US CDC (Center for Disease Control and Prevention) statistics, the average incidence of listeriosis in the US in 2003 was 3.3 / 1 million people. At present, there have been reports in China that Listeria monocytogenes has caused morbidity in pregnant women and resulted in fetal death. The b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01P1/00A23L1/015C12R1/01A23L5/20
Inventor 蔡俊鹏彭宗辉
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com