Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof

A technology for mononucleosis and Listeria, applied in the direction of bacteria, application, and microbial-based methods, can solve the problems of bacteriophage sterilization with large limitations, damage to food nutrients, color and aroma, etc., to achieve food safety Problems, high elimination rate, health-protecting effect

Inactive Publication Date: 2010-02-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary sterilization methods often cause bacterial residues. At the same time, food treated by traditional sterilization methods o

Method used

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  • Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof
  • Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof
  • Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof

Examples

Experimental program
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Effect test

Example Embodiment

[0066] Example 1

[0067] (1) Obtain Bdellovibrio BDSM08 by ultraviolet mutagenesis

[0068] The starting strain is Bdellovibrio BDS01, which was isolated from mud samples and deposited at the China Type Culture Collection in Wuhan University, Wuhan, China on August 7, 2009. The deposit number is CCTCC NO: M 209169 ; After negative staining, the morphological observation under an electron microscope: Budovibrus BDS01 is a single cell, arc-shaped, with a size of 0.9×0.25μm, terminal flagella, and a length of 4μm; the double-layer plate method is used at 28℃ After four days of cultivation, transparent round plaques with a diameter of 1-2 mm can be formed.

[0069] According to the application number "2008100220488.3", the method in the national invention patent application named "Ultraviolet-induced mutation in the enhancement of the lysis performance of Budovibrio", the Budovibrio BDS01 is used in the ultraviolet lamp power 15W, and the power of the auxiliary infrared light source is...

Example Embodiment

[0089] Example 2

[0090] (1) Use microbial preparations to eliminate Listeria monocytogenes carried by aquatic products before consumption

[0091] The test container is a 25L aquarium, which is divided into a test group and a control group, with 3 parallels in each group. The test water is fresh water for breeding, which is filtered by a filter membrane, and each aquarium is filled with 16L of filtered water. The aquarium was disinfected with sodium dichloroisocyanurate before the experiment, and the water temperature was 26°C. Add step (3) of Example 1 to each aquarium to prepare Listeria monocytogenes liquid, adjust with sterile water to make the final concentration 1.0×10 4 cfu / mL. Then put 10 tilapia and 10 prawns in each aquarium. The tilapia used in the experiment weighed about 140-190g, the shrimp weighed about 15-30g, and the body length was about 8-10cm. The control group was only the Listeria monocytogenes prepared in step (3)① of Example 1, and the final concentrat...

Example Embodiment

[0094] Example 3

[0095] The microbial preparation is used to eliminate Listeria monocytogenes carried by eggs, the specific process is as follows:

[0096] The materials used in the experiment were eggs, and the experiment was set up as a control group and a test group. There are three parallel samples, each with 10 eggs, and each egg weighs about 50g. Respectively immerse the eggs in step (3) of Example 1 to prepare a concentration of 10 9 In cfu / mL Listeria monocytogenes liquid, adjust the final concentration with sterile water to make the concentration reach 1.0×10 4 cfu / mL, take out and drain after 30 seconds. Then divide every 10 eggs into a small group and immerse them in the microbial preparations A, B, C, D, E, and F prepared in Example 1. The eggs were immersed for 30 seconds and then taken out and drained. They were placed at room temperature, and the content of Listeria monocytogenes was detected at two moments of 2 hours and 4 hours. The specific detection method o...

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Abstract

The invention discloses a bdellovibrio bacteriovorus bacterial strain for eliminating listeria monocytogenes and an application thereof. The invention obtains bdellovibrio bacteriovorus BDSM08 by separation, purification and ultraviolet mutation. BDSM08 is in the arc-shaped unicellular form when observed by an electron microscope, the size thereof is 1.8*1.0 mu m, the ends thereof are provided with flagellum and the length of the flagellum is 3.2 mu m. The bdellovibrio bacteriovorus BDSM08 is cultivated with the two layer plating method at the temperature of 28 DEG C for three days to form transparent round negativecolony the diameter of which is 2-3mm and the optimum growing pH value of which is 7.2, the optimum growing temperature of which is 28 DEG C and the optimum salinity of which is0%. The microbial agent prepared by BDSM08 has a strong effect on killing listeria monocytogenes which can be commonly seen in environment. The invention applies the bdellovibrio bacteriovorus BDSM08for the first time to prevent and cure food listerellosis; the invention has a favourable effect on eliminating listeria monocytogenes on food, has characteristics of no toxicity, no side effect on food and the like, and has favourable application prospect.

Description

technical field [0001] The invention relates to a Bdellovibrio, in particular to a Bdellovibrio strain for eliminating Listeria monocytogenes and application thereof. Background technique [0002] Listeria monocytogenes (Listeria monocytogenes), referred to as Listeria monocytogenes LM, is a zoonotic pathogen. It exists widely in nature and exists in most foods such as vegetables, milk and dairy products, raw meat, cooked meat products and aquatic products. Listeriosis can result from eating food contaminated with Listeria monocytogenes. Listeriosis is associated with a high mortality rate and is second only to salmonella infection as the most deadly foodborne disease. According to the US CDC (Center for Disease Control and Prevention) statistics, the average incidence of listeriosis in the US in 2003 was 3.3 / 1 million people. At present, there have been reports in China that Listeria monocytogenes has caused morbidity in pregnant women and resulted in fetal death. The b...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P1/00A23L1/015C12R1/01A23L5/20
Inventor 蔡俊鹏彭宗辉
Owner SOUTH CHINA UNIV OF TECH
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