13 results about "Fluorescence assay" patented technology

The utility model discloses an organic solvent evaporation device for fluorescence determination of food trace elements, which comprises a hot plate instrument, and the upper plate surface of an electric hot plate of the hot plate instrument is a hot plate surface of a heating evaporation dish; an air injection stirring pipe located above the electric hot plate is arranged on the hot plate instrument through a driving mechanism, the air inlet end of the air injection stirring pipe is connected with a hot air source supply system, the air outlet end of the air injection stirring pipe is in butt joint with an air distributor, and the air injection stirring pipe is driven by the driving mechanism to have a lifting motion state and a rotating motion state. In-out and rotary stirring relative to the evaporating dish are realized. According to the utility model, the air injection stirring pipe is arranged on the hot plate instrument to pneumatically stir a sample in the evaporation dish, so that the evaporation efficiency of an organic solvent in the sample can be effectively improved.

The invention provides a gossypol specific nucleic acid aptamer as well as a preparation method and application thereof. The nucleotide sequence of the aptamer is shown as SEQ ID NO. 1. A Capture-SELEX technology is adopted, a screening system is optimized, high-throughput sequencing and DNAMAN homology and secondary structure analysis are combined, the gossypol specific nucleic acid aptamer is obtained, ThT fluorescence determination and ITC verification show that the aptamer still keeps high affinity after truncation, and the gossypol specific nucleic acid aptamer can be used for preparing the gossypol specific nucleic acid aptamer. Molecular docking further defines that key basic groups such as G-26, C-27, G-28 and G-46 form a hydrogen bond-hydrophobic effect with gossypol, and a foundation is laid for construction of a subsequent gossypol rapid detection system.

The application discloses a method for fluorescent analysis of APE1 enzyme based on a three-dimensional multi-legged DNA walker constructed by double gold balls. AuNPs as a basis to construct two systems, one of which is to modify the walker chain to AuNPs according to 500:1 to serve as a walker, named AuNP-W; the other is to modify the Sub chain to AuNPs according to 1000:1 to serve as a track, named AuNP-T. In addition, endogenous ATP serves as the driving force for the continuous walking of the DNA walker. 2+ buffer to anneal AuNP-W and Block chain to form double-stranded AuNP-W / B; use TAE / Mg 2+ buffer to anneal AuNP-T and ATP aptamer chain Apt (containing FAM fluorophore) to form double-stranded AuNP-S / A; mix AuNP-W / B, AuNP-S / A and ATP according to a certain proportion to obtain a biosensor for fluorescent analysis and detection of APE1 enzyme, add APE1 enzyme with different concentrations, and perform real-time fluorescent quantitative analysis under the condition of 37 DEG C to measure the fluorescence curves corresponding to APE1 enzyme with different concentrations; add the sample to be measured into the biosensor for fluorescent analysis and detection of APE1, set the same detection conditions, measure the fluorescence curves of different samples to be measured, and obtain the specific detection results of APE1 enzyme.

A method for reducing spectral crosstalk in multiplex assays is provided. The method includes receiving filtered signal data from multiple fluorometry and an initial dye matrix including calibrated spectral dye data. The method further includes generating an updated dye matrix based on the initial dye matrix and the estimated crosstalk replacement value, and then adjusting the updated dye matrix to meet a calculated optimization value. The calculated optimization value is calculated based at least on the estimated crosstalk substitution value and the filtered signal data. The calculated optimization value may be further calculated based on each adjustment of the updated dye matrix and a cross-correlation between dyes used in the assay. The method further includes generating an improved dye matrix based on the adjusted updated dye matrix, and then generating spectral adjustment data based on the improved dye matrix.

The present disclosure relates to relates to compositions useful for probing activity and function of constitutive androstane receptor and kinases using fluorescence assays as disclosed herein. The present disclosure further relates to methods of using the disclosed compositions in methods for probing activity and function of constitutive androstane receptor and kinases. The present disclosure also pertains to kits comprising the disclosed compositions and instructions for using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

To provide a determination system for correcting optical information related to measurement of a measurement object based on difference of an apparatus to be used.SOLUTION: A determination system includes: a fluorescence determination element arranged at a position predetermined by a plurality of predetermined fluorescent parts; a light source for irradiating the fluorescence determination element by one excitation light; and a control part including image acquisition means for photographing the fluorescence determination element irradiated by the light source to acquire calibration data being image data, and determination means for determining fluorescence information based on the calibration data.SELECTED DRAWING: Figure 3A

The present invention provides a nitromethane fluorescence determination device, and relates to the technical field of nitromethane fluorescence determination, the nitromethane fluorescence determination device comprises a detection assembly, a material control assembly and a hydraulic assembly, the detection assembly comprises a material box I and a material box II, the bottom of the material box I is provided with a batching assembly, the batching assembly comprises a batching barrel and a raw material barrel, the invention discloses a fluorescence determination method and device for nitromethane, the device comprises a dosing barrel, the bottom of the dosing barrel is provided with a mixing assembly, the mixing assembly comprises a mixing barrel and a detection cylinder, the mixing barrel is provided with a determination assembly, and the determination assembly comprises a fluorescence detector and a sensor. The problems of missed recording, wrong recording, disordered taking, disordered use and the like are greatly reduced, and the device is suitable for rapid routing inspection work of the land.

The application discloses a fluorescence probe for measuring crystallinity of hydrotalcite colloidal sol based on pi-pi stacking regulation and a preparation method thereof. The application adopts an imidazole anchoring naphthalene diamine (IANA) fluorophore with a planar naphthalene motif as the fluorescence probe. The imidazole group has strong binding capacity for transition divalent metal ions, and the transition metal enhances the ordered arrangement of the IANA after coordination with the imidazole, which is conducive to the face-to-face pi-pi stacking of the planar naphthalene motif and inhibits the absorption of ultraviolet light, thereby causing fluorescence quenching. However, the internal structure order and spatial atomic arrangement of different crystallinity materials are different, which will cause differences in the ordered arrangement of the IANA, affect the quenching degree of the probe, and then the crystallinity can be measured through the quenching degree. The application regulates the fluorescence to measure the crystallinity of the hydrotalcite colloidal sol through the pi-pi stacking, and opens up a new way for exploring a replacement scheme of X-ray diffraction.

This invention discloses a novel bifunctional tyramine red-light carbon dot, its preparation method, and its applications. The novel bifunctional tyramine red-light carbon dot is a red-light carbon dot (Tyr-CDs) with tyramine structural residues on its surface. It is prepared using tyramine and o-phenylenediamine as precursors via a one-step hydrothermal method. The novel bifunctional tyramine red-light carbon dot possesses signal amplification and detection functions as well as cell fluorescence labeling functions. This carbon dot exhibits excellent luminescence performance, good stability, low preparation cost, a simple preparation method, and low biotoxicity. It can be combined with ELISA and TSA technologies to establish highly sensitive and low-cost immunofluorescence quantitative detection methods, and is expected to serve as a novel fluorescent probe to replace traditional fluorescent dyes. Furthermore, based on its excellent signal amplification and labeling functions, it can achieve ultra-high sensitivity fluorescence measurement of single yeast cells, providing new signal amplification reagents for biological techniques such as ELISA, cell fluorescence labeling, and immunohistochemistry, and has high application value in the field of biosensing.

The present disclosure relates to methods and kits for detecting free thiols in a substrate and methods for quantifying the amount of free thiols in a substrate. In particular, the present disclosure provides a fluorescent Ellman assay for improving the sensitivity of free thiols detection and quantification.

This invention disclosure presents a novel method and system for protein detection that significantly enhances accuracy by minimizing cross-excitation and cross-emission effects commonly encountered in multiplex fluorescence assays. The method involves isolating multiple proteins from a sample, labeling them with fluorescent inks, and arranging them on a sample substrate such that no adjacent labels have overlapping excitation or emission spectra. Each labeled protein is sequentially excited by a specifically matched laser wavelength and detected by a corresponding detector, equipped with adjustable optical filters, to ensure precise measurement of the fluorescence emission. The system comprises a high-sensitivity optical detection instrument that includes a light source, a detector, and a control unit. The control unit is designed to adjust the sequence of excitation and detection based on the detected fluorescence intensity, optimizing the dynamic range of the assay.This methodology and system setup allow for high-throughput protein detection with reduced spectral interference, providing a more reliable and efficient approach to quantifying multiple proteins in complex samples.

The invention relates to a method for determining α-arabinofuranosidase (abbreviated ABF) activity in a sample, referred to as the sample to be analyzed (p), of a product intended for animal feed and containing an additive that exhibits ABF activity, the ABF activity to be determined being specific to the additive, the method comprising the following steps: on the one hand, a sample, referred to as the control sample (c), of the product which is free of the additive, and, on the other hand, the sample to be analyzed (p), are provided; for each of the control sample (c) and the sample to be analyzed (p), respectively, an enzyme fraction exhibiting ABF activity is extracted under the same conditions, then the ABF activity of each of the extracts, respectively ABFc and ABFp, is measured; and ABFp - ABFc is calculated to obtain the specific ABF activity of the additive in the product; according to which method the ABF activity is measured by fluorescence by means of a substrate for the enzyme, 4-methylumbelliferyl-α-L-arabinofuranoside, which is hydrolyzable to fluorescent 4-methylumbelliferone.