36 results about "Fluorescence assay" patented technology

Provided is a fluorescence measuring device capable of measuring biopolymers such as proteins in real time at lower concentrations than in conventional devices. This fluorescence measurement probe is used by being connected by an optical fiber to a light source for irradiating a liquid being measured with excitation light and a detector for detecting fluorescence derived from the liquid being measured that is excited by the excitation light from the light source, the fluorescence measurement probe being provided with: a port for introducing the excitation light from the optical fiber and for guiding out fluorescence and scattered light from the liquid being measured to the optical fiber; a condensing optical system for collimating and / or condensing the excitation light, the fluorescence, and the scattered light; a polarizing plate transparent to the excitation light, the fluorescence, and the scattered light; and a housing for internally accommodating the condensing optical system and the polarizing plate and having an opening portion for injecting the excitation light into the liquid being measured and taking in the fluorescence and the scattered light from the liquid being measured.

A biological structure identification system includes an optical imaging system configured to illuminate a liquid biopsy sample for a subject. The liquid biopsy sample has one or more biological structures that are labeled with one or more fluorophores associated with a fluorescence assay for a cancer allowing detection of emitted electromagnetic radiation from the liquid biopsy sample as image data. The system also includes a processing system configured to identify a cancer stage for the subject by determining a predetermined cancer stage profile from a set of predetermined cancer stage profiles to which the subject profile is most similar.

The invention provides an interleukin 33 detection kit as well as a preparation method and application thereof. The interleukin 33 detection kit comprises a first antibody coupled to a magnetic fluorescent light-emitting microsphere, a second antibody labeled with biotin and fluorescein-labeled streptavidin, the first antibody strain and the second antibody strain are monoclonal antibodies for resisting interleukin 33, and interleukin 33 specific binding sites recognized by the first antibody strain and the second antibody strain are different. According to the kit disclosed by the invention, the interleukin 33 antigen is specifically combined with the magnetic fluorescent luminous microsphere formed by coupling the interleukin 33 capture antibody, then the magnetic fluorescent luminous microsphere is specifically combined with the biotin-labeled interleukin 33 detection antibody, the conjugate is combined with fluorescein-labeled streptavidin, fluorescence is emitted, and the content of the interleukin 33 in a sample to be detected is determined. The method is high in sensitivity, good in repeatability, short in reaction time, simple to operate and high in anti-interference performance.

The utility model discloses an organic solvent evaporation device for fluorescence determination of food trace elements, which comprises a hot plate instrument, and the upper plate surface of an electric hot plate of the hot plate instrument is a hot plate surface of a heating evaporation dish; an air injection stirring pipe located above the electric hot plate is arranged on the hot plate instrument through a driving mechanism, the air inlet end of the air injection stirring pipe is connected with a hot air source supply system, the air outlet end of the air injection stirring pipe is in butt joint with an air distributor, and the air injection stirring pipe is driven by the driving mechanism to have a lifting motion state and a rotating motion state. In-out and rotary stirring relative to the evaporating dish are realized. According to the utility model, the air injection stirring pipe is arranged on the hot plate instrument to pneumatically stir a sample in the evaporation dish, so that the evaporation efficiency of an organic solvent in the sample can be effectively improved.

Methods for selecting a microbe for co-formulation with a carrier are provided. In some examples, the methods include identifying a microbe that comprises one or more dipicolinic acid (DPA) synthase genes, a microbe that expresses one or more DPA synthase proteins, and / or a microbe that produces DPA; and selecting the microbe for co-formulation with a carrier. The methods optionally include co-formulating the selected microbe with the carrier. In some examples, the methods include detecting one or more DPA synthase genes or one or more DpaA and / or DpaB proteins in a microbe. In other examples, the methods include detecting DPA in a microbe or medium containing a microbe, for example, utilizing a fluorescence assay. Microbial compositions including one or more microbes that comprise one or more DPA synthase genes, express one or more DPA synthase proteins and / or produce DPA are also provided.

The present invention discloses a method for dual-ratio fluorescence detection of morphine in blood using nitrogen-doped carbon quantum dots; the present invention uses m-phenylenediamine and spermidine as carbon and nitrogen sources, and synthesizes nitrogen-doped fluorescent carbon quantum dots with high fluorescence quantum yield by microwave method; due to the fluorescence resonance energy transfer effect between morphine and carbon quantum dots, in the presence of morphine, the fluorescence of carbon quantum dots decreases at 350 nm, while increases sharply at 456 nm, and the ratio of the fluorescence intensity at 350 nm to that at 456 nm has a certain linear relationship with the morphine concentration, based on which a fluorescence spectrophotometry for detecting morphine in blood is established; the nitrogen-doped fluorescent carbon quantum dots prepared by the present invention have significant fluorescence sensitization and quenching effects on morphine, and have strong selectivity, and are used for the fluorescence determination of morphine in blood, with the characteristics of high sensitivity and specificity.

Methods for selecting a microbe for co-formulation with a carrier are provided. In some examples, the methods include identifying a microbe that comprises one or more dipicolinic acid (DPA) synthase genes, a microbe that expresses one or more DPA synthase proteins, and / or a microbe that produces DPA; and selecting the microbe for co-formulation with a carrier. The methods optionally include co-formulating the selected microbe with the carrier. In some examples, the methods include detecting one or more DPA synthase genes or one or more DpaA and / or DpaB proteins in a microbe. In other examples, the methods include detecting DPA in a microbe or medium containing a microbe, for example, utilizing a fluorescence assay. Microbial compositions including one or more microbes that comprise one or more DPA synthase genes, express one or more DPA synthase proteins and / or produce DPA are also provided.

The invention discloses an upconversion nanoprobe for detecting tyrosinase with dual signal output and application thereof, and relates to the technical field of nanobiomaterials. The upconversion nanoprobe comprises UCNPs, tyramine and iron ions; the UCNPs has a core-shell structure, with β-NaYF4:Yb,Er as the core and NaYF4 as the shell; the upconversion nanoprobe of the invention forms a dual-signal self-calibration capability by integrating the advantages of fluorescence determination method and colorimetry; the method not only utilizes the UCL signal excited by near-infrared light, greatly improving the detection accuracy, but also provides an intuitive and convenient method for the detection of tyrosinase through colorimetric reaction; the dual-signal output detection mode effectively alleviates signal fluctuations, thereby ensuring the accuracy and reliability of tyrosinase analysis.

The present invention relates to the field of environmental testing technology, and in particular to a fluorescence spectrometry method for determining the molecular weight of natural dissolved organic matter. The fluorescence spectrometry method for determining the molecular weight of natural dissolved organic matter comprises the following steps: (a) preparation of a natural dissolved organic matter test solution and a background solution; (b) selection of the fluorescence peak position in a titration experiment; (c) fluorescence quenching titration of the natural dissolved organic matter; (d) fluorescence measurement of the background solution; and (e) calculation of the molecular weight of the natural dissolved organic matter. This method is not only simple to operate, but also has low requirements for equipment and operating skills. Model compounds of natural dissolved organic matter molecules such as tyrosine and natural dissolved organic matter standard samples have been used to prove that the method results are accurate and reliable.

Methods and systems for determining the concentration of compounds, such as proteins, in compositions comprising adjuvanted complexes of the compounds are provided. The methods and systems generally comprise (i) measuring the intrinsic fluorescence of a composition comprising adjuvanted complexes of the compound and (ii) comparing the measured fluorescence intensity value to a calibration curve prepared using known concentrations of the compound. Also provided are methods and systems for determining percent adsorption of a selected compound to an adjuvant.

The invention discloses an allergen specificity IgE antibody flow fluorescence method detection kit and a detection method. The flow type fluorescence method detection kit for the allergen specificity IgE antibody comprises an antigen-microsphere compound working solution, a PE-secondary antibody compound working solution and a cleaning solution. Wherein the working solution contains APC fluorescence coding magnetic microspheres marked with different allergens and antigens, and different allergy indexes are detected according to different marked allergens and antigens. The PE-secondary antibody compound working solution contains an anti-human IgE antibody marked by PE fluorescent protein. The magnetic fluorescent coding microspheres with different numbers are respectively marked with different allergen antigen proteins, and then the coding microspheres marked with antigens are combined, so that quantitative detection of various allergen specific antibodies can be completed through one-time test, and the kit has the advantages of simultaneous detection of multiple indexes, low sample demand, wide linear range, high sensitivity, high sensitivity and the like. And quantification can be realized.

The invention provides a gossypol specific nucleic acid aptamer as well as a preparation method and application thereof. The nucleotide sequence of the aptamer is shown as SEQ ID NO. 1. A Capture-SELEX technology is adopted, a screening system is optimized, high-throughput sequencing and DNAMAN homology and secondary structure analysis are combined, the gossypol specific nucleic acid aptamer is obtained, ThT fluorescence determination and ITC verification show that the aptamer still keeps high affinity after truncation, and the gossypol specific nucleic acid aptamer can be used for preparing the gossypol specific nucleic acid aptamer. Molecular docking further defines that key basic groups such as G-26, C-27, G-28 and G-46 form a hydrogen bond-hydrophobic effect with gossypol, and a foundation is laid for construction of a subsequent gossypol rapid detection system.

Methods and systems for determining the concentration of compounds, such as proteins, in compositions comprising adjuvanted complexes of the compounds are provided. The methods and systems generally comprise (i) measuring the intrinsic fluorescence of a composition comprising adjuvanted complexes of the compound and (ii) comparing the measured fluorescence intensity value to a calibration curve prepared using known concentrations of the compound. Also provided are methods and systems for determining percent adsorption of a selected compound to an adjuvant.

The application discloses a method for fluorescent analysis of APE1 enzyme based on a three-dimensional multi-legged DNA walker constructed by double gold balls. AuNPs as a basis to construct two systems, one of which is to modify the walker chain to AuNPs according to 500:1 to serve as a walker, named AuNP-W; the other is to modify the Sub chain to AuNPs according to 1000:1 to serve as a track, named AuNP-T. In addition, endogenous ATP serves as the driving force for the continuous walking of the DNA walker. 2+ buffer to anneal AuNP-W and Block chain to form double-stranded AuNP-W / B; use TAE / Mg 2+ buffer to anneal AuNP-T and ATP aptamer chain Apt (containing FAM fluorophore) to form double-stranded AuNP-S / A; mix AuNP-W / B, AuNP-S / A and ATP according to a certain proportion to obtain a biosensor for fluorescent analysis and detection of APE1 enzyme, add APE1 enzyme with different concentrations, and perform real-time fluorescent quantitative analysis under the condition of 37 DEG C to measure the fluorescence curves corresponding to APE1 enzyme with different concentrations; add the sample to be measured into the biosensor for fluorescent analysis and detection of APE1, set the same detection conditions, measure the fluorescence curves of different samples to be measured, and obtain the specific detection results of APE1 enzyme.

A method for reducing spectral crosstalk in multiplex assays is provided. The method includes receiving filtered signal data from multiple fluorometry and an initial dye matrix including calibrated spectral dye data. The method further includes generating an updated dye matrix based on the initial dye matrix and the estimated crosstalk replacement value, and then adjusting the updated dye matrix to meet a calculated optimization value. The calculated optimization value is calculated based at least on the estimated crosstalk substitution value and the filtered signal data. The calculated optimization value may be further calculated based on each adjustment of the updated dye matrix and a cross-correlation between dyes used in the assay. The method further includes generating an improved dye matrix based on the adjusted updated dye matrix, and then generating spectral adjustment data based on the improved dye matrix.

The present disclosure relates to relates to compositions useful for probing activity and function of constitutive androstane receptor and kinases using fluorescence assays as disclosed herein. The present disclosure further relates to methods of using the disclosed compositions in methods for probing activity and function of constitutive androstane receptor and kinases. The present disclosure also pertains to kits comprising the disclosed compositions and instructions for using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

To provide a determination system for correcting optical information related to measurement of a measurement object based on difference of an apparatus to be used.SOLUTION: A determination system includes: a fluorescence determination element arranged at a position predetermined by a plurality of predetermined fluorescent parts; a light source for irradiating the fluorescence determination element by one excitation light; and a control part including image acquisition means for photographing the fluorescence determination element irradiated by the light source to acquire calibration data being image data, and determination means for determining fluorescence information based on the calibration data.SELECTED DRAWING: Figure 3A

The invention relates to the technical field of chemical detection, in particular to a method for measuring calcium, silicon and magnesium in quicklime through X-ray fluorescence with ignition loss correction, which comprises the following steps: preparing and drying a sample, burning the sample, calculating the amount of burning loss, fusing the sample, establishing an X-ray fluorescence detection method and setting detection conditions. The method comprises the following steps: establishing a working curve of X-ray fluorescence detection of quicklime, calibrating the working curve of X-ray fluorescence, carrying out ignition loss correction on a quicklime sample by an X-ray fluorescence method to obtain the content of the sample, and verifying the reliability of the working curve of X-ray fluorescence; before melting the glass sheet, the quick lime sample is fired, the ignition loss of the sample is calculated, the detection result is subjected to ignition loss correction calculation, the sample is subjected to glass melting sheet preparation, the content of calcium, silicon and magnesium is measured at one time by using the X-ray fluorescence spectrometry, chemical reagents and participated instruments are saved, the production cost is reduced, and the method is suitable for industrial production. The environmental pollution is reduced, and the production efficiency, accuracy and reliability are improved.

The present invention discloses a mercury atomic fluorescence analysis method and a mercury atomic fluorescence measuring instrument using water as a carrier, which belongs to the atomic fluorescence analysis in the field of analytical chemistry. The method includes the steps of sampling and infusion, reaction of a test solution and a reducing agent in a reactor to atomize mercury, generation of mercury atomic fluorescence and fluorescence detection, and in the conventional sampling and infusion process, water is used to replace HCl and a reducing agent as a carrier, so that the mercury test solution and reagent are pushed into the reactor by water to complete the reaction. The present invention uses water as a carrier, effectively overcomes the memory effect in mercury determination, improves the determination sensitivity and accuracy, and saves a large amount of high-purity HCl and reducing agent NaBH4 at the same time, greatly reduces the analysis cost, and significantly improves the operating environment, which is an innovation of mercury atomic fluorescence analysis technology.

The present invention provides a nitromethane fluorescence determination device, and relates to the technical field of nitromethane fluorescence determination, the nitromethane fluorescence determination device comprises a detection assembly, a material control assembly and a hydraulic assembly, the detection assembly comprises a material box I and a material box II, the bottom of the material box I is provided with a batching assembly, the batching assembly comprises a batching barrel and a raw material barrel, the invention discloses a fluorescence determination method and device for nitromethane, the device comprises a dosing barrel, the bottom of the dosing barrel is provided with a mixing assembly, the mixing assembly comprises a mixing barrel and a detection cylinder, the mixing barrel is provided with a determination assembly, and the determination assembly comprises a fluorescence detector and a sensor. The problems of missed recording, wrong recording, disordered taking, disordered use and the like are greatly reduced, and the device is suitable for rapid routing inspection work of the land.

An imaging structure identification system includes an optical imaging system configured to illuminate a liquid biopsy sample for a subject. The liquid biopsy sample has one or more biological structures that are labeled with one or more fluorophores associated with a fluorescence assay for a disease state allowing detection of emitted electromagnetic radiation from the liquid biopsy sample as image data. The system also includes a processing system configured to identifying the subject as being at reduced risk for having the disease state if the subject profile is similar to the first predetermined profile for subjects not being in the disease state. However, if the subject profile is not similar to the first predetermined profile for subjects not being in the disease state it is not determinative if the subject is in the disease state.

This invention relates to a detection method based on molecular biological fluorescence assay. Detection points are uniformly set within a test area. Samples are taken at these points, and several test samples are processed to obtain a detection template. This template is then added to a molecular fluorescence quantitative PCR reaction system to obtain detection results. Based on the results, positive and negative points are identified, a test area map is plotted, and positive points are marked. A preliminary positive concentration area is delineated based on the marked positive points in the test area map. This area is then magnified according to specified rules to obtain a detection amplification area. Detection points are rearranged within the detection amplification area, and the detection process is repeated. The final positive concentration area within the detection amplification area is obtained. Based on this final positive concentration area, the source region of the silkworm disease pathogen is analyzed. Furthermore, the pathogen's accumulation and transmission routes are analyzed. Based on the pathogen's accumulation and transmission routes, corresponding control areas are delineated, and matching control measures are implemented.

The present invention provides, inter alia, solutions to this problem, in particular, certain surfaces and certain sample holders to improve the sensitivity, speed and ease of use of optical signal based assays, such as colorimetric or fluorescence assays.

The present invention discloses a device for measuring the permeability coefficient of rock and soil, which relates to the field of geotechnical engineering technology. The device comprises a housing assembly, a water supply drive mechanism disposed inside the housing assembly, a sealing mechanism, a discharging mechanism, and a blocking mechanism disposed on the outside of the water supply drive mechanism in order from top to bottom, and a fluorescence measurement assembly disposed inside the housing assembly; the housing assembly comprises a sample holding housing, a drainage pipe, a soil output channel, a top plate, and a bracket; the fluorescence measurement assembly is fixedly disposed at the bottom of the inner cavity of the sample holding housing, the drainage pipe and the soil output channel are fixedly disposed through the left bottom of the sample holding housing in order from bottom to top, and the top plate is located directly above the sample holding housing. The present invention can automatically output the rock and soil sample after the measurement is completed, has a high degree of automation, saves manpower, and can effectively avoid soil residue, thereby allowing the next rock and soil sample to be tested quickly.

The application discloses a fluorescence probe for measuring crystallinity of hydrotalcite colloidal sol based on pi-pi stacking regulation and a preparation method thereof. The application adopts an imidazole anchoring naphthalene diamine (IANA) fluorophore with a planar naphthalene motif as the fluorescence probe. The imidazole group has strong binding capacity for transition divalent metal ions, and the transition metal enhances the ordered arrangement of the IANA after coordination with the imidazole, which is conducive to the face-to-face pi-pi stacking of the planar naphthalene motif and inhibits the absorption of ultraviolet light, thereby causing fluorescence quenching. However, the internal structure order and spatial atomic arrangement of different crystallinity materials are different, which will cause differences in the ordered arrangement of the IANA, affect the quenching degree of the probe, and then the crystallinity can be measured through the quenching degree. The application regulates the fluorescence to measure the crystallinity of the hydrotalcite colloidal sol through the pi-pi stacking, and opens up a new way for exploring a replacement scheme of X-ray diffraction.

The present invention discloses a method for improving the recombination efficiency of the Bxb1 enzyme by optimizing the enzyme. The method comprises at least the following steps: (1) selecting mutation sites that affect the activity of the Bxb1 enzyme based on the structure of the Bxb1 enzyme; (2) performing directed mutagenesis on the mutation sites selected in step (1) to obtain different Bxb1 enzyme mutants; and (3) establishing a fluorescence assay in host cells to evaluate the recombination efficiency of the different Bxb1 enzyme mutants in step (2). The present invention successfully obtained three Bxb1 enzyme mutants, namely Bxb1(L82W), Bxb1(D13R), and Bxb1(T15R), by using directed mutagenesis and fluorescence assay evaluation. Compared with the wild-type Bxb1 enzyme, the three Bxb1 enzyme mutants all showed improved cell line recombination efficiency.

The application discloses a heparin-binding protein nucleic acid aptamer fluorescence assay kit and belongs to the field of kits. The kit comprises a box body, a cover plate is hingedly connected to the top of the box body, a shaking mechanism is arranged on one side of the box body, the shaking mechanism comprises a fixing frame, the fixing frame is fixedly connected with the box body, a motor is fixedly connected to the inside of the fixing frame, an eccentric block is fixedly connected to the output end of the motor, a limiting mechanism is arranged in the inside of the cover plate, the eccentric block, the motor and the fixing frame are arranged, the motor is fixed through the fixing frame, the output end of the motor drives the eccentric block to rotate, the surface of the box body is knocked by the eccentric block to make the reagent tube in the inside of the box body vibrate, the liquid in the reagent tube is shaken, the heparin-binding protein nucleic acid aptamer fluorescence assay kit cannot shake the liquid in the reagent tube, and the problem that precipitates are prone to appearing in the liquid is solved, and the practicability is improved.

The present invention generally relates to systems and methods for imaging or determining nucleic acids, e.g., within cells. In some embodiments, a transcriptome of a cell may be determined. Certain embodiments relate to the determination of nucleic acids, such as mRNA, within a cell at relatively high resolution. In some embodiments, multiple nucleic acid probes may be applied to a sample and their binding within the sample, for example using fluorescence, to determine the location of the nucleic acid probes within the sample. In some embodiments, a codeword may be based on binding of multiple nucleic acid probes, and in some cases, the codeword may define an error correction code to reduce or prevent misidentification of nucleic acids. In some cases, a relatively large number of different targets may be identified using relatively small numbers of markers, such as by using various combination methods.

The utility model relates to the technical field of medical equipment, and discloses a high-flux flow type fluorescence determination device which comprises a main box body, a box body cover plate is clamped on the upper surface of the main box body, and a sheath liquid barrel, a water pump, a hose and a spray head are inserted on the upper surface of the box body cover plate. The sheath liquid detection device is reasonable in structure, sheath liquid is added into the sheath liquid barrel from the water inlet pipe on the upper surface of the sheath liquid cover plate by a worker, a detection sample is added into the sample hopper by the sample inlet plug on the upper surface of the sample cover plate, and the sample in the sheath liquid is detected by using different laser lamps; the problems that the detection amount of a sample is inconvenient to control each time during detection, detection is inaccurate, and grouping detection cannot be conducted for multiple times are avoided, a cleaning agent in a cleaning box is pumped into a hose through a water pump and sprayed out through a water outlet hole in the lower surface of a spray head, and the problems that cleaning is inconvenient after workers use the cleaning agent, the service life of equipment is shortened, and the working efficiency is improved are solved. The detection accuracy is reduced and the working efficiency is influenced because the equipment is not cleaned after long-time use.

The invention discloses a method for rapidly determining components of refining slag through X-ray fluorescence spectrometry, efficient detection of silicon dioxide, calcium oxide, magnesium oxide and aluminum oxide is realized through combination of chemical wet method valuing and optimization of sample preparation and instrument conditions, and the method specifically comprises the following steps: S1, taking a refining slag sample, crushing, and screening the refining slag sample with the particle size of not more than 180 meshes; s2, selecting a certain number of crushed and screened refining slag samples with different contents, and analyzing and valuing by using a chemical wet method to serve as standard samples; and S3, drawing a standard curve by utilizing the self-made standard sample, and measuring silicon dioxide, calcium oxide, magnesium oxide and aluminum oxide in the refining slag by utilizing X fluorescence. The method is short in analysis period, low in cost, good in stability and suitable for batch rapid analysis of the refining slag in steelmaking production, and the problems that a traditional method is low in efficiency and tedious in operation are solved.