23 results about "Fluorescence assay" patented technology

The invention provides an interleukin 33 detection kit as well as a preparation method and application thereof. The interleukin 33 detection kit comprises a first antibody coupled to a magnetic fluorescent light-emitting microsphere, a second antibody labeled with biotin and fluorescein-labeled streptavidin, the first antibody strain and the second antibody strain are monoclonal antibodies for resisting interleukin 33, and interleukin 33 specific binding sites recognized by the first antibody strain and the second antibody strain are different. According to the kit disclosed by the invention, the interleukin 33 antigen is specifically combined with the magnetic fluorescent luminous microsphere formed by coupling the interleukin 33 capture antibody, then the magnetic fluorescent luminous microsphere is specifically combined with the biotin-labeled interleukin 33 detection antibody, the conjugate is combined with fluorescein-labeled streptavidin, fluorescence is emitted, and the content of the interleukin 33 in a sample to be detected is determined. The method is high in sensitivity, good in repeatability, short in reaction time, simple to operate and high in anti-interference performance.

The utility model discloses an organic solvent evaporation device for fluorescence determination of food trace elements, which comprises a hot plate instrument, and the upper plate surface of an electric hot plate of the hot plate instrument is a hot plate surface of a heating evaporation dish; an air injection stirring pipe located above the electric hot plate is arranged on the hot plate instrument through a driving mechanism, the air inlet end of the air injection stirring pipe is connected with a hot air source supply system, the air outlet end of the air injection stirring pipe is in butt joint with an air distributor, and the air injection stirring pipe is driven by the driving mechanism to have a lifting motion state and a rotating motion state. In-out and rotary stirring relative to the evaporating dish are realized. According to the utility model, the air injection stirring pipe is arranged on the hot plate instrument to pneumatically stir a sample in the evaporation dish, so that the evaporation efficiency of an organic solvent in the sample can be effectively improved.

The present invention discloses a method for dual-ratio fluorescence detection of morphine in blood using nitrogen-doped carbon quantum dots; the present invention uses m-phenylenediamine and spermidine as carbon and nitrogen sources, and synthesizes nitrogen-doped fluorescent carbon quantum dots with high fluorescence quantum yield by microwave method; due to the fluorescence resonance energy transfer effect between morphine and carbon quantum dots, in the presence of morphine, the fluorescence of carbon quantum dots decreases at 350 nm, while increases sharply at 456 nm, and the ratio of the fluorescence intensity at 350 nm to that at 456 nm has a certain linear relationship with the morphine concentration, based on which a fluorescence spectrophotometry for detecting morphine in blood is established; the nitrogen-doped fluorescent carbon quantum dots prepared by the present invention have significant fluorescence sensitization and quenching effects on morphine, and have strong selectivity, and are used for the fluorescence determination of morphine in blood, with the characteristics of high sensitivity and specificity.

The present invention relates to the field of environmental testing technology, and in particular to a fluorescence spectrometry method for determining the molecular weight of natural dissolved organic matter. The fluorescence spectrometry method for determining the molecular weight of natural dissolved organic matter comprises the following steps: (a) preparation of a natural dissolved organic matter test solution and a background solution; (b) selection of the fluorescence peak position in a titration experiment; (c) fluorescence quenching titration of the natural dissolved organic matter; (d) fluorescence measurement of the background solution; and (e) calculation of the molecular weight of the natural dissolved organic matter. This method is not only simple to operate, but also has low requirements for equipment and operating skills. Model compounds of natural dissolved organic matter molecules such as tyrosine and natural dissolved organic matter standard samples have been used to prove that the method results are accurate and reliable.

Methods and systems for determining the concentration of compounds, such as proteins, in compositions comprising adjuvanted complexes of the compounds are provided. The methods and systems generally comprise (i) measuring the intrinsic fluorescence of a composition comprising adjuvanted complexes of the compound and (ii) comparing the measured fluorescence intensity value to a calibration curve prepared using known concentrations of the compound. Also provided are methods and systems for determining percent adsorption of a selected compound to an adjuvant.

The invention provides a gossypol specific nucleic acid aptamer as well as a preparation method and application thereof. The nucleotide sequence of the aptamer is shown as SEQ ID NO. 1. A Capture-SELEX technology is adopted, a screening system is optimized, high-throughput sequencing and DNAMAN homology and secondary structure analysis are combined, the gossypol specific nucleic acid aptamer is obtained, ThT fluorescence determination and ITC verification show that the aptamer still keeps high affinity after truncation, and the gossypol specific nucleic acid aptamer can be used for preparing the gossypol specific nucleic acid aptamer. Molecular docking further defines that key basic groups such as G-26, C-27, G-28 and G-46 form a hydrogen bond-hydrophobic effect with gossypol, and a foundation is laid for construction of a subsequent gossypol rapid detection system.

The application discloses a method for fluorescent analysis of APE1 enzyme based on a three-dimensional multi-legged DNA walker constructed by double gold balls. AuNPs as a basis to construct two systems, one of which is to modify the walker chain to AuNPs according to 500:1 to serve as a walker, named AuNP-W; the other is to modify the Sub chain to AuNPs according to 1000:1 to serve as a track, named AuNP-T. In addition, endogenous ATP serves as the driving force for the continuous walking of the DNA walker. 2+ buffer to anneal AuNP-W and Block chain to form double-stranded AuNP-W / B; use TAE / Mg 2+ buffer to anneal AuNP-T and ATP aptamer chain Apt (containing FAM fluorophore) to form double-stranded AuNP-S / A; mix AuNP-W / B, AuNP-S / A and ATP according to a certain proportion to obtain a biosensor for fluorescent analysis and detection of APE1 enzyme, add APE1 enzyme with different concentrations, and perform real-time fluorescent quantitative analysis under the condition of 37 DEG C to measure the fluorescence curves corresponding to APE1 enzyme with different concentrations; add the sample to be measured into the biosensor for fluorescent analysis and detection of APE1, set the same detection conditions, measure the fluorescence curves of different samples to be measured, and obtain the specific detection results of APE1 enzyme.

A method for reducing spectral crosstalk in multiplex assays is provided. The method includes receiving filtered signal data from multiple fluorometry and an initial dye matrix including calibrated spectral dye data. The method further includes generating an updated dye matrix based on the initial dye matrix and the estimated crosstalk replacement value, and then adjusting the updated dye matrix to meet a calculated optimization value. The calculated optimization value is calculated based at least on the estimated crosstalk substitution value and the filtered signal data. The calculated optimization value may be further calculated based on each adjustment of the updated dye matrix and a cross-correlation between dyes used in the assay. The method further includes generating an improved dye matrix based on the adjusted updated dye matrix, and then generating spectral adjustment data based on the improved dye matrix.

The present disclosure relates to relates to compositions useful for probing activity and function of constitutive androstane receptor and kinases using fluorescence assays as disclosed herein. The present disclosure further relates to methods of using the disclosed compositions in methods for probing activity and function of constitutive androstane receptor and kinases. The present disclosure also pertains to kits comprising the disclosed compositions and instructions for using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

To provide a determination system for correcting optical information related to measurement of a measurement object based on difference of an apparatus to be used.SOLUTION: A determination system includes: a fluorescence determination element arranged at a position predetermined by a plurality of predetermined fluorescent parts; a light source for irradiating the fluorescence determination element by one excitation light; and a control part including image acquisition means for photographing the fluorescence determination element irradiated by the light source to acquire calibration data being image data, and determination means for determining fluorescence information based on the calibration data.SELECTED DRAWING: Figure 3A

The present invention provides a nitromethane fluorescence determination device, and relates to the technical field of nitromethane fluorescence determination, the nitromethane fluorescence determination device comprises a detection assembly, a material control assembly and a hydraulic assembly, the detection assembly comprises a material box I and a material box II, the bottom of the material box I is provided with a batching assembly, the batching assembly comprises a batching barrel and a raw material barrel, the invention discloses a fluorescence determination method and device for nitromethane, the device comprises a dosing barrel, the bottom of the dosing barrel is provided with a mixing assembly, the mixing assembly comprises a mixing barrel and a detection cylinder, the mixing barrel is provided with a determination assembly, and the determination assembly comprises a fluorescence detector and a sensor. The problems of missed recording, wrong recording, disordered taking, disordered use and the like are greatly reduced, and the device is suitable for rapid routing inspection work of the land.

This invention relates to a detection method based on molecular biological fluorescence assay. Detection points are uniformly set within a test area. Samples are taken at these points, and several test samples are processed to obtain a detection template. This template is then added to a molecular fluorescence quantitative PCR reaction system to obtain detection results. Based on the results, positive and negative points are identified, a test area map is plotted, and positive points are marked. A preliminary positive concentration area is delineated based on the marked positive points in the test area map. This area is then magnified according to specified rules to obtain a detection amplification area. Detection points are rearranged within the detection amplification area, and the detection process is repeated. The final positive concentration area within the detection amplification area is obtained. Based on this final positive concentration area, the source region of the silkworm disease pathogen is analyzed. Furthermore, the pathogen's accumulation and transmission routes are analyzed. Based on the pathogen's accumulation and transmission routes, corresponding control areas are delineated, and matching control measures are implemented.

The present invention discloses a device for measuring the permeability coefficient of rock and soil, which relates to the field of geotechnical engineering technology. The device comprises a housing assembly, a water supply drive mechanism disposed inside the housing assembly, a sealing mechanism, a discharging mechanism, and a blocking mechanism disposed on the outside of the water supply drive mechanism in order from top to bottom, and a fluorescence measurement assembly disposed inside the housing assembly; the housing assembly comprises a sample holding housing, a drainage pipe, a soil output channel, a top plate, and a bracket; the fluorescence measurement assembly is fixedly disposed at the bottom of the inner cavity of the sample holding housing, the drainage pipe and the soil output channel are fixedly disposed through the left bottom of the sample holding housing in order from bottom to top, and the top plate is located directly above the sample holding housing. The present invention can automatically output the rock and soil sample after the measurement is completed, has a high degree of automation, saves manpower, and can effectively avoid soil residue, thereby allowing the next rock and soil sample to be tested quickly.

The application discloses a fluorescence probe for measuring crystallinity of hydrotalcite colloidal sol based on pi-pi stacking regulation and a preparation method thereof. The application adopts an imidazole anchoring naphthalene diamine (IANA) fluorophore with a planar naphthalene motif as the fluorescence probe. The imidazole group has strong binding capacity for transition divalent metal ions, and the transition metal enhances the ordered arrangement of the IANA after coordination with the imidazole, which is conducive to the face-to-face pi-pi stacking of the planar naphthalene motif and inhibits the absorption of ultraviolet light, thereby causing fluorescence quenching. However, the internal structure order and spatial atomic arrangement of different crystallinity materials are different, which will cause differences in the ordered arrangement of the IANA, affect the quenching degree of the probe, and then the crystallinity can be measured through the quenching degree. The application regulates the fluorescence to measure the crystallinity of the hydrotalcite colloidal sol through the pi-pi stacking, and opens up a new way for exploring a replacement scheme of X-ray diffraction.

The present invention discloses a method for improving the recombination efficiency of the Bxb1 enzyme by optimizing the enzyme. The method comprises at least the following steps: (1) selecting mutation sites that affect the activity of the Bxb1 enzyme based on the structure of the Bxb1 enzyme; (2) performing directed mutagenesis on the mutation sites selected in step (1) to obtain different Bxb1 enzyme mutants; and (3) establishing a fluorescence assay in host cells to evaluate the recombination efficiency of the different Bxb1 enzyme mutants in step (2). The present invention successfully obtained three Bxb1 enzyme mutants, namely Bxb1(L82W), Bxb1(D13R), and Bxb1(T15R), by using directed mutagenesis and fluorescence assay evaluation. Compared with the wild-type Bxb1 enzyme, the three Bxb1 enzyme mutants all showed improved cell line recombination efficiency.

The application discloses a heparin-binding protein nucleic acid aptamer fluorescence assay kit and belongs to the field of kits. The kit comprises a box body, a cover plate is hingedly connected to the top of the box body, a shaking mechanism is arranged on one side of the box body, the shaking mechanism comprises a fixing frame, the fixing frame is fixedly connected with the box body, a motor is fixedly connected to the inside of the fixing frame, an eccentric block is fixedly connected to the output end of the motor, a limiting mechanism is arranged in the inside of the cover plate, the eccentric block, the motor and the fixing frame are arranged, the motor is fixed through the fixing frame, the output end of the motor drives the eccentric block to rotate, the surface of the box body is knocked by the eccentric block to make the reagent tube in the inside of the box body vibrate, the liquid in the reagent tube is shaken, the heparin-binding protein nucleic acid aptamer fluorescence assay kit cannot shake the liquid in the reagent tube, and the problem that precipitates are prone to appearing in the liquid is solved, and the practicability is improved.

The utility model relates to the technical field of medical equipment, and discloses a high-flux flow type fluorescence determination device which comprises a main box body, a box body cover plate is clamped on the upper surface of the main box body, and a sheath liquid barrel, a water pump, a hose and a spray head are inserted on the upper surface of the box body cover plate. The sheath liquid detection device is reasonable in structure, sheath liquid is added into the sheath liquid barrel from the water inlet pipe on the upper surface of the sheath liquid cover plate by a worker, a detection sample is added into the sample hopper by the sample inlet plug on the upper surface of the sample cover plate, and the sample in the sheath liquid is detected by using different laser lamps; the problems that the detection amount of a sample is inconvenient to control each time during detection, detection is inaccurate, and grouping detection cannot be conducted for multiple times are avoided, a cleaning agent in a cleaning box is pumped into a hose through a water pump and sprayed out through a water outlet hole in the lower surface of a spray head, and the problems that cleaning is inconvenient after workers use the cleaning agent, the service life of equipment is shortened, and the working efficiency is improved are solved. The detection accuracy is reduced and the working efficiency is influenced because the equipment is not cleaned after long-time use.

The invention discloses a method for rapidly determining components of refining slag through X-ray fluorescence spectrometry, efficient detection of silicon dioxide, calcium oxide, magnesium oxide and aluminum oxide is realized through combination of chemical wet method valuing and optimization of sample preparation and instrument conditions, and the method specifically comprises the following steps: S1, taking a refining slag sample, crushing, and screening the refining slag sample with the particle size of not more than 180 meshes; s2, selecting a certain number of crushed and screened refining slag samples with different contents, and analyzing and valuing by using a chemical wet method to serve as standard samples; and S3, drawing a standard curve by utilizing the self-made standard sample, and measuring silicon dioxide, calcium oxide, magnesium oxide and aluminum oxide in the refining slag by utilizing X fluorescence. The method is short in analysis period, low in cost, good in stability and suitable for batch rapid analysis of the refining slag in steelmaking production, and the problems that a traditional method is low in efficiency and tedious in operation are solved.

This invention discloses a novel bifunctional tyramine red-light carbon dot, its preparation method, and its applications. The novel bifunctional tyramine red-light carbon dot is a red-light carbon dot (Tyr-CDs) with tyramine structural residues on its surface. It is prepared using tyramine and o-phenylenediamine as precursors via a one-step hydrothermal method. The novel bifunctional tyramine red-light carbon dot possesses signal amplification and detection functions as well as cell fluorescence labeling functions. This carbon dot exhibits excellent luminescence performance, good stability, low preparation cost, a simple preparation method, and low biotoxicity. It can be combined with ELISA and TSA technologies to establish highly sensitive and low-cost immunofluorescence quantitative detection methods, and is expected to serve as a novel fluorescent probe to replace traditional fluorescent dyes. Furthermore, based on its excellent signal amplification and labeling functions, it can achieve ultra-high sensitivity fluorescence measurement of single yeast cells, providing new signal amplification reagents for biological techniques such as ELISA, cell fluorescence labeling, and immunohistochemistry, and has high application value in the field of biosensing.

The present disclosure relates to methods and kits for detecting free thiols in a substrate and methods for quantifying the amount of free thiols in a substrate. In particular, the present disclosure provides a fluorescent Ellman assay for improving the sensitivity of free thiols detection and quantification.

This invention disclosure presents a novel method and system for protein detection that significantly enhances accuracy by minimizing cross-excitation and cross-emission effects commonly encountered in multiplex fluorescence assays. The method involves isolating multiple proteins from a sample, labeling them with fluorescent inks, and arranging them on a sample substrate such that no adjacent labels have overlapping excitation or emission spectra. Each labeled protein is sequentially excited by a specifically matched laser wavelength and detected by a corresponding detector, equipped with adjustable optical filters, to ensure precise measurement of the fluorescence emission. The system comprises a high-sensitivity optical detection instrument that includes a light source, a detector, and a control unit. The control unit is designed to adjust the sequence of excitation and detection based on the detected fluorescence intensity, optimizing the dynamic range of the assay.This methodology and system setup allow for high-throughput protein detection with reduced spectral interference, providing a more reliable and efficient approach to quantifying multiple proteins in complex samples.

The application discloses a method for quickly identifying mandelic acid enantiomers by using chiral fluorescent molecular cages, and utilizes the aggregation-induced emission (AIE) characteristics of tetramethoxy tetraphenyl ethylene to study the recognition performance of the molecular cages on the enantiomers of the chiral acid guest. A solvent is prepared by mixing tetrahydrofuran (THF) and water in a certain proportion, tetramethoxy tetraphenyl ethylene molecular cages are added, and then the fluorescence of different kinds of chiral acid guests is determined. The chiral self-adaptive recognition of the tetramethoxy tetraphenyl ethylene fluorescent molecular cages provided by the application to different amino acid molecules can quickly identify mandelic acid enantiomers. By adding the solvent to the mandelic acid, whether the mandelic acid is L type or D type can be quickly distinguished, and a new possibility is provided for the recognition of amino acids based on the chiral fluorescent molecular cages.

The invention relates to a method for determining α-arabinofuranosidase (abbreviated ABF) activity in a sample, referred to as the sample to be analyzed (p), of a product intended for animal feed and containing an additive that exhibits ABF activity, the ABF activity to be determined being specific to the additive, the method comprising the following steps: on the one hand, a sample, referred to as the control sample (c), of the product which is free of the additive, and, on the other hand, the sample to be analyzed (p), are provided; for each of the control sample (c) and the sample to be analyzed (p), respectively, an enzyme fraction exhibiting ABF activity is extracted under the same conditions, then the ABF activity of each of the extracts, respectively ABFc and ABFp, is measured; and ABFp - ABFc is calculated to obtain the specific ABF activity of the additive in the product; according to which method the ABF activity is measured by fluorescence by means of a substrate for the enzyme, 4-methylumbelliferyl-α-L-arabinofuranoside, which is hydrolyzable to fluorescent 4-methylumbelliferone.