Beta-diketone-trivalent europium complex nano fluorescent probe, its preparation and use thereof

Abstract

This invention relates to a kind of beta- diketone-triatomic europium complex nanometer fluorescent probe and its preparation and application. Its steps are the following: the monomer which can copolymarized with silicate ester takes conjugated bonding reaction with beta- diketone-triatomic europium complex in organic solvent and then takes copolymerization with silicate ester to form functional nanometer rare earth fluorescence particles. The said triatomic europium ion, beta - diketone organic complex and the monomer and silicate ester take the mol proportion as 1 : 2-3 : 10-100 : 350-450. The particles is of higher fluorescence and stability and has in its surface with active function group directly used for biological marking and is free of interference of scattered light and short light when used for detecting time-resolution fluorescent. This invention is of high value in fields of time-resolution fluorescent immune detecting, cell chemistry, microscope forming, DNA crossing detecting and biological chip detecting.

Description

technical field [0001] The invention relates to a nanometer rare earth fluorescent probe, in particular to a beta-diketone-trivalent europium complex nanometer (rare earth) fluorescent probe and its preparation and application. Background technique [0002] The rapid development of medicine and life sciences has put forward a large number of new topics for analytical chemistry. The analysis and determination of nucleic acid, polypeptide, protein and other biological macromolecules, biological drugs, microorganisms, ultra-trace biologically active substances, etc. Therefore, bioanalytical chemistry has become one of the most important frontier research fields in the field of international analytical science; based on the needs of the above situation, many new analytical methods and technologies have been continuously developed, such as nano-fluorescence detection. Needle technology, biochip technology, nanochannel technology, molecular imprinting technology, molecular beacon ...

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Problems solved by technology

[0006] (2) Semiconductor nanocrystals (also known as quantum dots) (Document 3: W.C.W.Chan, S.Nie, Science, 1998, 281, 2016-2018; Document 4: Y.W.Cao, Angew.Chem.Int.Ed., 1999, 38, 3692-3694): The invention of quantum dots created a new field of application of nano-luminescent particles as markers; the quantum dots reported in the literature are mainly compounds of II-VI and III-V elements, such as CdSe, InP and InAs, etc.; quantum dots have a wide range of excitation light, sharp emission peaks, and large Stokes shifts. The quantum yield of individual quantum dots, such as CdSe / CdS nanocrystals, can even reach 100%. Under the excitation of excitation light, the color of quantum dots luminescence Depending on its size, almost all wavelengths of light from blue to red can be obtained; the combination of surface-modified quantum dots and biomolecules can be used as fluorescent probes for laser scanning imaging and immunoassays; quantum dots The disadvantage of fluorescent probes is that the luminescence is unstable, easy to blink, and does not have sufficient monodispersity

Since this kind of nano-rare-earth fluorescent probe is prepared by encapsulating some rare-earth fluorescent complexes in silica gel nanoparticles, in the process of surface modification and labeling biomolecules, it may be due to the modification of the silica gel shell, etc. The reason leads to the leakage of the encapsulated rare earth fluorescent complex, which weakens the fluorescence and reduces the sensitivity of the assay.

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