Beta-diketone-trivalent europium complex nano fluorescent probe, its preparation and use thereof

A nano-fluorescent probe and trivalent europium technology, which is applied in the field of nano-rare earth fluorescent probes, can solve the problems of weakened fluorescence, easy flickering, and reduced measurement sensitivity, so as to solve the problem of fluorescent bleaching, enhance photostability, and high luminous intensity Effect

Inactive Publication Date: 2005-01-19
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF3 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (2) Semiconductor nanocrystals (also known as quantum dots) (Document 3: W.C.W.Chan, S.Nie, Science, 1998, 281, 2016-2018; Document 4: Y.W.Cao, Angew.Chem.Int.Ed., 1999, 38, 3692-3694): The invention of quantum dots created a new field of application of nano-luminescent particles as markers; the quantum dots reported in the literature are mainly compounds of II-VI and III-V elements, such as CdSe, InP and InAs, etc.; quantum dots have a wide range of excitation light, sharp emission peaks, and large Stokes shifts. The quantum yield of individual quantum dots, such as CdSe/CdS nanocrystals, can even reach 100%. Under the excitation of excitation light, the color of quantum dots luminescence Depending on its size, almost all wavelengths of lig...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Beta-diketone-trivalent europium complex nano fluorescent probe, its preparation and use thereof
  • Beta-diketone-trivalent europium complex nano fluorescent probe, its preparation and use thereof
  • Beta-diketone-trivalent europium complex nano fluorescent probe, its preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Covalently bonded 4,4'-bis(1", 1", 1", 2", 2", 3", 3"-heptafluoro-4", 6"-hexanedione-6"-yl) Preparation and characterization of fluorescent nanoparticles of chlorosulfonyl-o-diphenylbenzene (abbreviated as BHHCT)-europium complex:

[0045] (1) Covalently bonded BHHCT-Eu 3+ Preparation of fluorescent nanoparticles of the complex:

[0046] Add BHHCT to cyclohexane (refer to literature 11 for the synthetic method: J.Yuan, K.Matsumoto, H.Kimura, Anal.Chem., 1998, 70, 596-601) and aminopropyltriethoxysilane (abbreviated as APS ), the molar ratio of BHHCT to APS is 1:5 to 1:50, preferably 1:7 to 1:18, the molar concentration of BHHCT in the system is 0.05 to 0.9 μmol / ml, stirred and reacted for 2 hours at room temperature, then added BHHCT molar Europium trichloride (EuCl 3 ), ultrasonic vibration for 20 minutes, the prepared APS-BHHCT-Eu 3+ The combination is preserved for future use. Mix cyclohexane, n-octanol, Triton X-100 and water in a certain proportion (molar rati...

Embodiment 2

[0058] Using covalently bonded BHHCT-Eu 3+ The time-resolved fluorescent immunoassay method using complex nano fluorescent particles as markers is used to determine the hepatitis B surface antigen (HBsAg for short) in human serum.

[0059] (1) Use nano-rare earth fluorescent particles to label streptavidin (SA for short)

[0060] Disperse 1 mg of nanoparticles into 1.0 ml of 0.1 mol / L phosphate buffer solution (pH=7.1), oscillate with ultrasonic waves for 20 minutes, add 5 mg of bovine serum albumin (BSA for short), and add 300 μl of 1% pentadiene under stirring Aldehyde (phosphate buffer solution), react at room temperature for 24 hours, and collect the precipitate by centrifugation. The precipitate was washed twice with 200 μl of phosphate buffer solution, then added to 200 μl of 1% glutaraldehyde (phosphate buffer solution), then 1 mg of SA was added, and after 24 hours of reaction at room temperature, 0.5 mg of NaBH was added 4 Reduction, reacted at room temperature for ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention relates to a kind of beta- diketone-triatomic europium complex nanometer fluorescent probe and its preparation and application. Its steps are the following: the monomer which can copolymarized with silicate ester takes conjugated bonding reaction with beta- diketone-triatomic europium complex in organic solvent and then takes copolymerization with silicate ester to form functional nanometer rare earth fluorescence particles. The said triatomic europium ion, beta - diketone organic complex and the monomer and silicate ester take the mol proportion as 1 : 2-3 : 10-100 : 350-450. The particles is of higher fluorescence and stability and has in its surface with active function group directly used for biological marking and is free of interference of scattered light and short light when used for detecting time-resolution fluorescent. This invention is of high value in fields of time-resolution fluorescent immune detecting, cell chemistry, microscope forming, DNA crossing detecting and biological chip detecting.

Description

technical field [0001] The invention relates to a nanometer rare earth fluorescent probe, in particular to a beta-diketone-trivalent europium complex nanometer (rare earth) fluorescent probe and its preparation and application. Background technique [0002] The rapid development of medicine and life sciences has put forward a large number of new topics for analytical chemistry. The analysis and determination of nucleic acid, polypeptide, protein and other biological macromolecules, biological drugs, microorganisms, ultra-trace biologically active substances, etc. Therefore, bioanalytical chemistry has become one of the most important frontier research fields in the field of international analytical science; based on the needs of the above situation, many new analytical methods and technologies have been continuously developed, such as nano-fluorescence detection. Needle technology, biochip technology, nanochannel technology, molecular imprinting technology, molecular beacon ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/64G01N33/52
Inventor 袁景利谭明乾海晓丹王桂兰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap