The invention discloses steel for bucket teeth of construction machinery and a preparation method of the bucket teeth, and relates to the technical field of mechanism manufacturing. The steel for the bucket teeth is prepared from the following substances in percentage by weight: 0.25 to 0.33% of C, 0.30 to 0.80% of Si, 0.80 to 1.20% of Mn, 0.60 to 1.0% of Cr, 0.030 to 0.036% of Re, 0.0005 to 0.0035% of B, 0.20 to 0.40% of nanometer TiN, not greater than 0.030% of S, and not greater than 0.030% of P. With the adoption of the method, the problems of poor impact toughness, easy breaking, small service life, and high production cost which is caused by lots of added precious metals Mo and Ni can be solved.