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Fluid cell equipment-microcarrier clinical diagnosis chip

A technology of flow cytometry and clinical diagnosis, applied in the field of flow cytometry-microcarrier clinical diagnosis chip, which can solve problems such as confusion, technical complexity, and affecting the affinity of biomolecules

Inactive Publication Date: 2006-11-01
林远
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, flow cytometry-microcarrier technology has not yet constituted a real challenge to traditional immunoassay methods, mainly due to the following aspects: the surface of polymer particles is treated with chemical fluorescent paint (BD Company of the United States; Luminex Company of the United States), and the technology is complicated. , and will affect its affinity as a carrier for biomolecules; when using multivalent harvesting and analysis, several or dozens of different polymer particles appear in the same two-dimensional coordinate map, often overlapping, confusing and other complex More importantly, the effective diagnosis of clinical diseases not only requires a large number of random, but also selective screening of relevant factors

Method used

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  • Fluid cell equipment-microcarrier clinical diagnosis chip
  • Fluid cell equipment-microcarrier clinical diagnosis chip
  • Fluid cell equipment-microcarrier clinical diagnosis chip

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] Preparation of microcarriers - biomolecules (antigens / antibodies)

[0026] The preparation of microcarrier-antigen / antibody complexes involves binding protein molecules such as antigens / antibodies to the surface of polymer particles through covalent bonds. The diameter of the polymer particles can be 1-100 microns, preferably 1-50 microns, most ideally 1-20 microns. The material of polymer particles can vary according to needs, for example, latex, silicon, resin, and various plastic polymer materials, including styrene, bromostyrene, acrylic acid, acrylic amine, methacrylate, vinyl chloride, Polyurethane and polymerizable monomers made of chlorinated styrene, ethylene acetate, etc. The covalent bonding between polymer particles and antigens / antibodies is accomplished through groups such as epoxy resin, acetaldehyde, and carbon diamine. It can also be accomplished by intermediate molecules carrying amine groups or other reactive groups such as succinyl esters. Unbound...

example 1

[0036] Example 1 Infectious Disease Detection System

[0037] According to one of the specific embodiments of the present invention, latex particles of one diameter are respectively bound to at least 6 kinds of purified pathogen antigens or antibodies through covalent bonding. Then, the protein molecules bound to the six kinds of latex particles were labeled with different intensities of autofluorescence (such as PE from 1 μM to 10 μM) to form a fluorescence gradient (such as from 0 level to 5 level). Incubate at least one autofluorescent latex particle, or latex particle with at least one diameter, with a biological sample (such as human serum, culture fluid) to bind the ligand to be tested. The ligand to be tested bound to the latex particles was then labeled with FITC-goat anti-human IgG / IgM / IgA. The size of latex particles was determined by electron density (vertical axis) versus forward light diffraction (horizontal axis). Select latex particles with a single diameter a...

example 2

[0057] Example 2. Allergic test system

[0058] According to another specific embodiment of the present invention, latex particles of one diameter are respectively bound to six purified allergens through covalent bonding. Then six kinds of protein molecules bound to the latex particles were labeled with different intensities of autofluorescence (such as PE from 1 μM to 10 μM) to form a fluorescence gradient (such as from 0 level to 5 level). At least one autofluorescent latex particle, or at least one diameter of latex particle, is incubated with a biological sample (eg, human serum) to bind to a specific ligand, anti-allergen IgE. The anti-allergen IgE bound to the latex particles was then labeled with FITC-goat anti-human IgE. See Example 1 for flow cytometry analysis.

[0059] latex particles

(diameter)

autofluorescence

(level)

Microcarriers - Antigen / Antibody

detection antibody

1-20μm

0

anti-human IgE

anti-huma...

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Abstract

A method for preparing flow cytometer -micro carrier clinical diagnosis chip includes fixing different biological molecules on micro carrier then labeling them with fluorescence in different intensity to form a fluorescent gradient, using micro carrier-biological molecule to culture with sample and combining it with ligand, confirming micro carrier with different size and obtaining micro carrier in different diameter region when flow cytometer is on gravest, selecting micro carrier in different size and using its own fluorescence to compare with detection fluorescence for determining ligand to be tested, providing said chip for clinically diagnose infectivity disease, taraxy disease, autoimmune disease, etc..

Description

Technical field [0001] The invention relates to the technique, material and medicine box for rapidly diagnosing various diseases using flow cytometer-microcarrier technology. Background technique [0002] Accurate and rapid antigen-antibody detection is of great significance to the clinical diagnosis and treatment of many diseases, especially those diseases with high incidence and threat to human life. Examples include infectious diseases, allergic diseases, immune diseases and tumors. The diagnosis of many infectious diseases relies heavily on the isolation of pathogens and the serological examination of antibodies. Direct antigen detection is of great value for early diagnosis. However, in many cases, it is first necessary to culture the specimen to amplify the pathogen, that is, the indirect antigen detection method. Serological antibody determination has important diagnostic value for many infections, such as human immunodeficiency virus (HIV) infection, SARS, and epi...

Claims

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Application Information

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IPC IPC(8): G01N33/53C09K11/00G01N33/533G01N33/546
Inventor 林远
Owner 林远
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