37 results about "Antibiotic susceptibilities" patented technology

The invention provides a single bacterium drug sensitivity detection method and system, and relates to the technical field of drug sensitivity detection.The single bacterium drug sensitivity detection method comprises the steps that a cultured to-be-tested strain is subjected to fluorescent staining and then mixed with an antibiotic solution, and a mixed solution is obtained; continuing the strain to be tested in the mixed solution through a structured light illumination microscope to obtain an original imaging sequence; reconstructing and preprocessing each original imaging sequence to obtain a processed imaging sequence, and then cutting out a single bacterium to be tested from each processed image in the processed imaging sequence to obtain a plurality of region-of-interest sequences; and inputting each region-of-interest sequence into a pre-trained evaluation model to obtain a detection result including drug resistance or susceptibility. The method has the beneficial effects that the method focuses on the early change of a subcellular structure before bacterial division, so that the detection time is greatly shortened; imaging acquisition and image processing and cutting are carried out on the single bacterium level, and the survival (drug resistance prompting) state and the non-survival (susceptibility prompting) state of single bacteria can be rapidly distinguished.

The present disclosure relates to filters configured for a spectroscopic antibiotic susceptibility analysis and a method for filtering a sample for a spectroscopic antibiotic susceptibility analysis. In one example, a method comprises providing a solution comprising bacteria incubated in a media to a well, filtering the solution via adsorption to trap at least a portion of the bacteria in a filter separate from the media. providing a solution of SERS active nanoparticles to the well, and spectroscopically analyzing the filter to determine the presence and / or absence of at least one of a purine and a pyrimidine. Methods of providing an internal standard in a spectroscopic antibiotic susceptibility test are also provided.

Systems and methods for the detection of sepsis and treatment of patients. A sepsis detection system includes a first subsystem configured to detect the presence of an infection in a patient, a second subsystem configured to detect the presence of a dysregulated immune response in the patient, a third subsystem configured to detect organ dysfunction in the patient, a fourth subsystem configured to detect antibiotic resistance (ARB) of a pathogen in the patient, and a processing device. The first, second, third, and fourth subsystems and the processing device are communicatively coupled to each other via a network. The processing device is configured to determine the presence of sepsis in the patient based on the presence of infection, the presence of the dysregulated host response, and clinical data indicative of organ dysfunction in the patient.The subsystems utilize at least one polymerase chain reaction (PCR) procedure, Raman spectroscopy, clinical data, electronic health record (EHR) data, and antibiotic susceptibility testing (antibiogram).

The present disclosure relates to a method of determining antibiotic susceptibility of a micro-organism in a sample, the method including: receiving the sample containing the micro-organisms; incubating at least one first portion of the sample with at least one antibiotic and at least one second portion of the sample with no antibiotic; extracting nucleic acid from the at least one first portion of the sample and the at least one second portion of the sample, wherein the nucleic acid is associated with the micro-organisms present in the sample; amplifying the extracted nucleic acid from the at least one first portion and the at least one second portion of the sample; and obtaining the antibiotic susceptibility information associated with the micro-organism from the amplified nucleic acid from the at least one first portion and the at least one second portion of the sample.

The invention belongs to the field of biological medicine, and particularly relates to a mass spectrum tag probe for a rapid drug sensitive test as well as preparation and application thereof, and the mass spectrum tag probe mainly comprises three parts, namely a gold nanoparticle carrier, a pegylated antibiotic target head and a mass spectrum tag peptide fragment. The probe disclosed by the invention is low in preparation cost, good in stability and high in detection speed (only 4 hours), and compared with a traditional drug sensitive test, the probe disclosed by the invention can be used for directly detecting a complex bacterial sample and does not need to be purified and cultured; compared with biosensing and mass spectrometry technologies, the probe provided by the invention has higher detection sensitivity and is not easily influenced by a matrix; compared with a qPCR technology, the method does not need to know the sequence information of the drug-resistant gene in advance, can directly detect the apparent sensitivity / drug resistance of bacteria, and is wider in detection range; compared with biosensing, mass spectrometry, qPCR and other methods, the probe provided by the invention can realize detection of various pathogenic bacteria and detection of sensitivity of multiple antibiotic drugs.

The invention discloses an antibacterial drug sensitivity test device which comprises a test bed, a vortex mixer, a support and a uniform spreading frame, a plurality of culture dishes are arranged on the test bed, alcohol lamps are arranged beside the culture dishes, a first test tube, a second test tube and a third test tube are arranged on the support, and the first test tube, the second test tube and the third test tube are arranged on the uniform spreading frame. Tweezers, a rubber head suction tube and an inoculation ring are sequentially arranged on the right side of the third test tube, and a motor is mounted on the outer side of the uniform spreading frame. A bacterial colony solution is injected into a culture dish, the culture dish is placed on a dish fixing seat, a bending column is rotated to enable a rotating disc to rotate, the culture dish is fixed by a rubber block, and the bacterial colony solution is uniformly spread in the culture dish through a uniform spreading frame, so that irregular growth of later experimental bacterial colonies caused by an ignored area is avoided; meanwhile, a comparison group and an antibiotic paper sheet group are arranged and a biofilm breaker group is added in the experiment, so that the accuracy of the experiment is enhanced, the hysteresis of the antibiotics is shown, and the inundation use of the antibiotics caused by errors of experimental data is avoided.

1. Name of the product of this design: An electronic device having an operation display portion with a cascaded graphical user interface for constructing an antibiotic sensitivity test for microorganisms. 2. Purpose of this design product: This design product is used for an electronic device. 3. The key design features of this design product lie in the content requiring protection of the graphical user interface. 4. The picture or photo that best illustrates the key points of the design: main view. 5. Purpose of the graphical user interface: Overall purpose of the interface: For microorganisms in patient samples, clinicians use the interface to determine the drugs to which the microorganisms are susceptible and build cascades for candidate drug families. If the microorganisms show resistance to drugs in the previous layer, users can create multiple layers to prevent over-selection of antibiotics to prevent the microorganisms from developing resistance. The part of the interface is the operation display part of the cascade graphical user interface. The buttons on the left side of the interface allow users to add new cascades or view existing cascades; the two buttons in the upper right corner of the interface are used to delete and save cascades; users can enter the cascade name in the first rectangular box from the left at the top, click the second rectangular box to select the biological interpretation group, and click the third rectangular box to select the style type; users can edit the drug family name by clicking the pen icon in the middle of the interface, click the "Select drug" rectangular box to select a specific drug, and click the "+" rectangular box on the right to add a new drug cascade; click the "+" rectangular box at the bottom to add a new drug family. After the user clicks the rectangular box "Select drug" in the middle of the main view to select a drug, the interface changes to Change State Figure 1; after the user clicks the rectangular box "Select drug" in Change State Figure 1 to select a drug, the interface changes to Change State Figure 2; after the user clicks the rectangular box with a "+" symbol on the middle right of the interface in Change State Figure 2, the interface changes to Change State Figure 3; after the user clicks the rectangular box "Select drug" in the second column in the middle of Change State Figure 3 to select a drug, the interface changes to Change State Figure 4; after the user clicks the rectangular box with a "+" symbol on the middle right of Change State Figure 4, the interface changes to Change State Figure 5; after the user clicks the rectangular box with a "+" symbol at the bottom of the interface in Change State Figure 5 to add a new drug family, the interface changes to Change State Figure 6; after the user clicks the rectangular box "Select drug" in the second group of drug families in Change State Figure 6 to select a drug, the interface changes to Change State Figure 7; after the user clicks the rectangular box with a "+" symbol at the bottom of Change State Figure 7 to add a new drug family, the interface changes to Change State Figure 8. 6. Other situations that require explanation: The contents in the dotted part of the figure are not subject to protection.

Provided herein is an antibiotic susceptibility test and related compositions, methods and systems based on detection of a nucleic acid from a target microorganism in a sample in the presence or absence of a lysis treatment of the sample.

The present disclosure provides methods for predicting drug resistance in a subject with a Pseudomonas infection based on the detection of antibiotic-induced mutational signatures of Pseudomonas species.

To provide efficient and automated methods and systems that obtain a sample of microorganism from a colony and subject the obtained sample to multiple tests.SOLUTION: A method comprises an automated step of locating and selecting a colony of microorganisms on a culture dish, an automated step of obtaining a sample of the selected colony of microorganisms, and an automated step of automatically preparing a suspension of a sample of microorganisms by submerging in the suspension a pick tool having the sample, subsequently, the pick tool being vibrated in at least the vertical direction to release in the suspension the sample from the pick tool. An apparatus and a system optionally enable downstream processing of samples prepared for antibiotic susceptibility testing (AST).SELECTED DRAWING: Figure 2

Provided herein are methods of determining antibiotic susceptibility of bacteria. In some embodiments, the methods include obtaining a series of images from partitioned samples over a length of time using a large volume scattering imaging (LVSi) technique to produce a set of image data. In some embodiments, the methods include extracting multiple phenotypic features from individual bacterial cells in the partitioned samples in parallel with one another and detecting one or more phenotypic feature changes associated with bacterial growth and response to the antibiotics in the set of image data over a selected period of time to produce a set of extracted phenotypic features, and passing the set of extracted phenotypic features through a trained machine learning model that predicts the identity and the antibiotic susceptibility of the bacterial cells in the partitioned samples. Related kits, devices, systems, computer readable media, and additional methods are also provided.

1. Name of the product in this design: Operation display unit of a cascaded graphical user interface for microbial antibiotic susceptibility testing of an electronic device. 2. Purpose of this design: An electronic device. 3. The key design features of this product are: the content that the graphical user interface requires protection for. 4. The picture or photo that best illustrates the key design points: Design 1 front view. 5. Design 1 is designated as the basic design. 6. Uses of the graphical user interface: Overall use of the interface: For microorganisms in patient samples, clinicians use the interface to identify drugs that the microorganisms are susceptible to, build cascades for candidate drug families, and create multiple tiers if the microorganisms show resistance to drugs in the previous tier. The part of the interface is the operation display section of the cascaded graphical user interface. The buttons on the left side of the interface allow users to add new cascades and view existing cascades; the two buttons in the upper right corner allow users to delete and save cascades; users can enter the cascade name in the first rectangle from the left at the top, select the biological interpretation group by clicking the second rectangle, and select the style type by clicking the third rectangle; users can select a specific drug by clicking the "Select drug" rectangle in the interface, add a new drug family by clicking the rectangle with the "+" symbol on the right; and add a new cascade by clicking the "+" rectangle at the bottom. After a user clicks the "Select drug" rectangle in the main view of Design 1 to select a drug, the interface changes to Design 1's modified state diagram 1. The user can then click the delete icon to the right of the name to delete the drug. After the user clicks the "Select drug" rectangle below Drug Family 1 in Design 1's modified state diagram 1, the interface changes to Design 1's modified state diagram 2. After the user clicks the rectangle with a "+" symbol in the middle right of Design 1's modified state diagram 2, the interface changes to Design 1's modified state diagram 3. After the user clicks the "Select drug" rectangle below Drug Family 2 in Design 1's modified state diagram 3, the interface changes to Design 1's modified state diagram 4. After the user clicks the rectangle with a "+" symbol in the middle right of Design 1's modified state diagram 4, the interface changes to Design 1's modified state diagram 5. After the user clicks the rectangle with a "+" symbol at the bottom of Design 1's modified state diagram 5 to add a new cascade, the interface changes to Design 1's modified state diagram 6. After the user clicks the "Select" rectangle in the second cascade of Design 1's modified state diagram 6... After selecting a drug using the "drug" rectangle, the interface changes to Design 1, state diagram 7. When the user clicks the rectangle with the "+" symbol at the bottom of Design 1, state diagram 7, to add a new cascade, the interface changes to Design 1, state diagram 8. The change process of Design 2 is the same as Design 1. 7. Other matters to be noted: The dotted line part of the figure is not claimed.

This image is a graphical user interface for displaying antibiotic susceptibility results. The images shown in Figures 1-9 are evolving images. That is, as information about the antibiotic susceptibility testing cascade is entered, additional fields appear, and as these fields are filled in, additional fields appear until the cascade is complete.

Various methods, devices, and systems for preparing an output sample of a defined concentration are disclosed. The output sample can be used for downstream tests such as downstream anti-infective or antibiotic susceptibility testing (AST). The method can comprise diluting an aliquot of a source sample comprising an infectious agent to yield a diluted sample; exposing one or more sensors to the diluted sample, wherein at least a part of each of the one or more sensors is in fluid communication with the diluted sample; incubating the diluted sample at an incubation temperature; monitoring a change in a solution characteristic of the diluted sample using a parameter analyzer or a computing device coupled to the one or more sensors; and cooling the diluted sample to a cooling temperature when the concentration of infectious agents within the diluted sample reaches the defined concentration to yield the output sample.

This image is a graphical user interface for displaying antibiotic susceptibility results. The images shown in Figures 1-9 are evolving images. That is, as information about the antibiotic susceptibility testing cascade is entered, additional fields appear, and as these fields are filled in, additional fields appear until the cascade is complete.

Provided herein is a rapid amplifiable readout of bacterial growth, such as an ELISA paired with metabolic labeling. Methods comprise a) contacting a sample from a subject suffering from a bacterial infection with an antibiotic; and b) determining the effect, if any, of the antibiotic on incorporation of a single- or di-D-amino acid into peptidoglycan of the bacterial cell or incorporation of trehalose into the bacterial cell envelope, wherein an antibiotic that reduces, as compared to control without the antibiotic being present, incorporation of the D-amino acid into peptidoglycan of the bacterial cell or inhibited incorporation of trehalose into the bacterial cell envelope is an antibiotic that is effective.

1. The name of the design product: the operation display part of cascade graphical user interface for building antibiotic susceptibility test of microorganism of electronic equipment. 2. The use of the design product: for an electronic equipment. 3. The design points of the design product: the protected content of the graphical user interface. 4. The picture or photo that best shows the design points: design 1 front view. 5. Design 1 is specified as the basic design. 6. The use of the graphical user interface: the overall use of the interface: for the microorganism in the patient sample, the clinician determines the drug to which the microorganism is susceptible through the interface, builds a cascade for the candidate drug family, and if the microorganism shows drug resistance to the previous level of drug, the user can create multiple levels to prevent over-selection of antibiotics to prevent the microorganism from developing drug resistance. The local part of the interface is the operation display part of the cascade graphical user interface. The left button of the interface is for the user to add a new cascade and view the existing cascade; the two buttons in the upper right corner of the interface can be used by the user to delete and save the cascade; the user can input the cascade name in the first rectangular box from the left in the upper part, select the biological interpretation group by clicking the second rectangular box, and select the style type by clicking the third rectangular box; the user can select a specific drug by clicking the rectangular box with "Select drug" in the interface, and add a new drug family by clicking the rectangular box with "+" symbol on the right; the user can add a new cascade by clicking the "+" rectangular box in the lower part. After the user clicks the rectangular box with "Select drug" in the middle of the design 1 main view to select a drug, the interface becomes design 1 change state diagram 1, and the user can click the delete icon to the right of the name to delete the drug; after the user clicks the rectangular box with "Select drug" below Drug Family 1 in design 1 change state diagram 1 to select a drug, the interface becomes design 1 change state diagram 2; after the user clicks the rectangular box with "+" symbol in the middle of the right of the interface of design 1 change state diagram 2, the interface becomes design 1 change state diagram 3; after the user clicks the rectangular box with "Select drug" below Drug Family 2 in design 1 change state diagram 3 to select a drug, the interface becomes design 1 change state diagram 4; after the user clicks the rectangular box with "+" symbol in the middle of the right of the interface of design 1 change state diagram 4, the interface becomes design 1 change state diagram 5; after the user clicks the rectangular box with "+" symbol in the lower part of the interface of design 1 change state diagram 5 to add a new cascade, the interface becomes design 1 change state diagram 6; after the user clicks the rectangular box with "Select drug" in the second cascade of design 1 change state diagram 6 to select a drug, the interface becomes design 1 change state diagram 7; after the user clicks the rectangular box with "+" symbol in the lower part of design 1 change state diagram 7 to add a new cascade, the interface becomes design 1 change state diagram 8. The change process of design 2 is the same as that of design 1. 7. Other matters needing explanation Other explanations: The content of the dashed line part in the figure is not claimed.

Disclosed are an antibiotic susceptibility testing device and a method thereof, in which before the testing device is activated to carry out testing, a growth recognition light source is operated to illuminate culture elements, and a light inspection module receives a growth message obtained from the number of the original specimen so as to determine if cultivation is completed. Then, the specimen is diluted and introduced into reaction receptacles of a reagent disc, of which the entirety is placed into an inspection setup tank to start the testing. After reaction, first and second inspection light sources of different wavelengths are sequentially applied to illuminate the same reaction receptacle. And, finally, a spectrum processing module converts a color message obtained from an AST-dedicated indicator into a wavelength message, to thereby obtain the result of inspection.

This image is a graphical user interface for displaying antibiotic susceptibility results. The images shown in Figures 1-9 are evolving images. That is, as information about the antibiotic susceptibility testing cascade is entered, additional fields appear, and as these fields are filled in, additional fields appear until the cascade is complete.

A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provide for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.

A method for increasing susceptibility of microorganisms to antibiotics includes providing a microorganism; administering a single type of an antibiotic including a nitroimidazole compound to the microorganism; and administering any of an uronic, aldonic, ulosonic, and aldaric feedstock to the microorganism. The feedstock is adapted to promote cell metabolism and antibiotic activation, and inhibit antibiotic inactivation pathways in the microorganism causing increased sensitivity of the microorganism to the nitroimidazole.

According to one aspect of the present invention, an electronic device for performing antibiotic sensitivity detection of a microorganism may comprise: a processor for: obtaining antibiotic data, the antibiotic data being data measured according to a change in an electrical characteristic of a target microorganism under antibiotic treatment; judging whether the antibiotic data is positive or negative, and inputting the positive control group data, the negative control group data and the antibiotic data of the target microorganism into a trained deep learning model, the deep learning model judges whether the antibiotic data is positive or negative according to the positive control group data, the negative control group data and the antibiotic data of the target microorganism; and identifying antibiotic sensitivity information of the target microorganisms according to different antibiotic types and concentrations by using the judgment result.

Provided is an antibiotic susceptibility evaluation apparatus including a sample unit including a sample and at least an antibiotic disk arranged on the sample, a light source radiating coherent light to the sample unit, an image sensor detecting transmitted light passing through the sample unit to obtain a sample image, and a controller configured to receive a first sample image, which is an image of the sample at an initial time when the antibiotic disk is arranged on the sample unit, and a second sample image, which is an image of the sample after a preset time, to obtain a spatial correlation of an interference pattern between the first sample image and the second sample image, and to evaluate susceptibility of the sample to antibiotics based on the spatial correlation according a position.

The present disclosure relates to a microchamber device (1) for antibiotic sensitivity testing of bacterial cells (12) of a range of bacterial cell sizes, the microchamber device (1) being arranged on a substrate (40). The invention relates to a microchamber device (1) comprising a microchamber (11), a fluid inlet (14) and a fluid outlet (16) in fluid communication with the microchamber (11), and an integrated sensor (18) arranged in the microchamber (11), the sensor (18) being configured to monitor properties of bacterial cell activity in the microchamber (11) in real time and characterized in that the microchamber (11) comprises at least one trapping device (20), the trapping device (20) being configured to trap bacterial cells in the microchamber (11). And at least one sensor configured to capture at least one bacterial cell (12) in the microchamber (11). The present disclosure also relates to a test system (2) comprising at least one microchamber device (1) and a method for performing an antibiotic sensitivity test on bacterial cells (12).

Disclosed are a sensing device and a method for diagnosing antibiotic susceptibility using same. The sensing device, according to one aspect of the present invention, may comprise an accommodation module, a first plate member, and a second plate member. The method for diagnosing antibiotic susceptibility, according to another aspect of the present invention, may comprise the steps of: (a) coating a sensor with an antibiotic; (b) injecting a fluid containing a microorganism into the accommodation module; and (c) detecting an electrical signal of the fluid to diagnose susceptibility of the microorganism to the antibiotic.

The invention relates to a reagent disc structure for antibiotic susceptibility testing, comprising a test holder (1), at least two support and positioning parts (112), a central injection portion (2), several microflow channels (3), several reaction receptacles (4), several preloaded reagents (41) for antibiotic susceptibility testing, several overflow channels (5), several overflow sumps (6), several narrowing portions (51), and several vent holes (7). Before packaging, the reaction receptacles (4) of the test holder (1) are preloaded with lyophilized preloaded reagents (41). A user can directly position the test holder (1) in a test device after injecting the sample into the central injection portion (2).During activation, the sample is subjected to centrifugal force, causing it to flow uniformly through the microflow channels (3) into the reaction receptacles (4). Figure for the abstract: 1.

The invention belongs to the field of gene detection, and particularly relates to a characteristic gene combination for predicting antibiotic susceptibility phenotype of shigella sonnei, a kit and application of the characteristic gene combination. The drug sensitive phenotype of shigella sonnei to antibiotics is judged by detecting specific genes or mutation, the method has the advantages of high accuracy, high sensitivity and high specificity, and accurate diagnosis and treatment of infection are facilitated.

The present disclosure relates to a method of determining antibiotic susceptibility of a micro-organism in a sample, the method including: receiving the sample containing the micro-organisms; incubating at least one first portion of the sample with at least one antibiotic and at least one second portion of the sample with no antibiotic; extracting nucleic acid from the at least one first portion of the sample and the at least one second portion of the sample, wherein the nucleic acid is associated with the micro-organisms present in the sample; amplifying the extracted nucleic acid from the at least one first portion and the at least one second portion of the sample; and obtaining the antibiotic susceptibility information associated with the micro-organism from the amplified nucleic acid from the at least one first portion and the at least one second portion of the sample.

Imaging method for earliest microbial growth detection. This method uses images to determine colony biomass, and the colony biomass determines when colonies can be picked for identification or analysis of antibiotic susceptibility testing. If the sample source is not a pure sample source, additional incubation may be required to allow an increase in colony biomass before picking.

The present invention relates to a rapid antibiotic susceptibility test and an identification method capable of rapidly providing information on antibiotics suitable for microorganisms by performing an antibiotic susceptibility test and an identification test in a single process. The present invention provides a rapid antibiotic susceptibility test and an identification method comprising the steps of: (a) transferring a sample taken from a patient to a first reaction space in which a sample separation means is present; (b) separating and concentrating the pathogen in the sample from the patient's sample by capturing them with the separation means within the first reaction space; and (c) performing the identification of the pathogen or an antibiotic susceptibility test.