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Method for using (CNTs/PANI)n-ITO anode-based MFC (Microbial Fuel Cell) biosensors in antibiotic susceptibility tests

A biosensor and drug susceptibility test technology, applied in the field of microbial fuel cell biosensor detection of drugs, can solve the problems of cumbersome operation, long detection time, poor repeatability, etc., to avoid cumbersome operation, improve detection sensitivity, and avoid long detection time Effect

Active Publication Date: 2017-10-20
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention proposes a new method for the traditional antibiotic susceptibility test method, which avoids the problems of long detection time, cumbersome operation and poor repeatability of the microbial plate method, and the constructed microbial fuel cell biosensor is easy to operate, and can realize fast, Real-time, high-sensitivity drug susceptibility testing

Method used

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  • Method for using (CNTs/PANI)n-ITO anode-based MFC (Microbial Fuel Cell) biosensors in antibiotic susceptibility tests
  • Method for using (CNTs/PANI)n-ITO anode-based MFC (Microbial Fuel Cell) biosensors in antibiotic susceptibility tests
  • Method for using (CNTs/PANI)n-ITO anode-based MFC (Microbial Fuel Cell) biosensors in antibiotic susceptibility tests

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Experimental program
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Effect test

Embodiment 1

[0031] 1) Immerse the ITO (indium tin oxide) electrode treated with 4% NaOH solution in 25mL of anhydrous toluene solution, add about 1.1g of APTES (3-aminopropyltriethoxysilane), in a nitrogen atmosphere After standing at room temperature for 16 hours, the ITO electrode was taken out, ultrasonically cleaned with toluene, ethanol and deionized water in sequence, and finally dried with nitrogen;

[0032] 2) Use a mixed solution of saturated concentrated nitric acid and concentrated sulfuric acid (v / v, 1:3) to ultrasonically acidify CNTs (carbon nanotubes) for 24 hours, centrifuge, and wash with ultrapure water until the supernatant is neutral; The CNTs obtained by centrifugation were lyophilized and stored, and then prepared into a 1 mg / mL CNTs suspension with ultrapure water;

[0033] 3) Dissolve 100mg of PANI (polyaniline) and 100mg of HCSA (camphorsulfonic acid) in 20mL of chloroform, stir until the solvent is completely evaporated, and then use ultrapure water to ultrasonic...

Embodiment 2

[0038] 1) Immerse the ITO electrode treated with 4% NaOH solution in 25mL of anhydrous toluene solution, add about 1.1g of APTES, place it in a nitrogen atmosphere at room temperature for 16h, take out the ITO electrode, and wash it with toluene, ethanol and deionized Ultrasonic cleaning with water, and finally drying with nitrogen;

[0039] 2) Use a mixed solution of saturated concentrated nitric acid and concentrated sulfuric acid (v / v, 1:3) to ultrasonically acidify CNTs for 24 hours, centrifuge, and wash with ultrapure water until the supernatant is neutral; CNTs obtained by centrifugation Freeze-dried and stored, and then prepared into 1mg / mL CNTs suspension with ultrapure water;

[0040] 3) Dissolve 100 mg of PANI and 100 mg of HCSA in 20 mL of chloroform, stir until the solvent is completely evaporated, and then ultrasonically disperse with ultrapure water to prepare a HCSA-doped PANI solution with a concentration of 1 mg / mL;

[0041] 4) Soak the ITO electrode obtained...

Embodiment 3

[0045] 1) Immerse the ITO electrode treated with 4% NaOH solution in 25mL of anhydrous toluene solution, add about 1.1g of APTES, place it in a nitrogen atmosphere at room temperature for 16h, take out the ITO electrode, and wash it with toluene, ethanol and deionized Ultrasonic cleaning with water, and finally drying with nitrogen;

[0046] 2) Use a mixed solution of saturated concentrated nitric acid and concentrated sulfuric acid (v / v, 1:3) to ultrasonically acidify CNTs for 24 hours, centrifuge, and wash with ultrapure water until the supernatant is neutral; CNTs obtained by centrifugation Freeze-dried and stored, and then prepared into 1mg / mL CNTs suspension with ultrapure water;

[0047] 3) Dissolve 100 mg of PANI and 100 mg of HCSA in 20 mL of chloroform, stir until the solvent is completely evaporated, and then ultrasonically disperse with ultrapure water to prepare a HCSA-doped PANI solution with a concentration of 1 mg / mL;

[0048] 4) Soak the ITO electrode obtained...

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Abstract

The invention discloses a method for using (CNTs / PANI)n-ITO anode-based MFC (Microbial Fuel Cell) biosensors in antibiotic susceptibility tests. A layer-by-layer self-assembly method is adopted to prepare a carbon nanotube / polyaniline compound-modified IT anode, which is used for constructing single-chamber and double-chamber microbial fuel cell biosensors, and the single-chamber and double-chamber microbial fuel cell biosensors are applied in antibiotic susceptibility tests on drugs such as gentamicin. The invention proposes a new method for conventional antibiotic susceptibility test methods, the problems of long assay time, complex operation, poor repetitiveness and the like existing in the microbial plate method are solved, the constructed microbial fuel cell biosensors are easy to operate, and moreover, a quick, real-time and high-sensitivity antibiotic susceptibility test can be carried out.

Description

technical field [0001] The invention belongs to the technical field of drug detection by microbial fuel cell biosensors, and in particular relates to a method for using a (CNTs / PANI)n-ITO anode-based MFC biosensor for a drug sensitivity test. Background technique [0002] In recent years, the abuse of antibiotics has seriously threatened human life and health. Traditional antibiotic susceptibility testing methods include disc diffusion method, agar dilution method, E test method and broth dilution method. However, these methods have the disadvantages of long time-consuming (24-48h) and cumbersome operation, and cannot meet the needs of clinical rapid diagnosis and treatment. Microbial fuel cells (MFC) use microorganisms as catalysts to convert chemical energy directly into biosensors as a device that converts electrical energy, and are used in fields such as power generation and energy supply, sewage treatment, environmental bioremediation, and biosensors. Microbial fuel c...

Claims

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Application Information

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IPC IPC(8): G01N27/413H01M8/16
CPCG01N27/413H01M8/16Y02E60/50Y02P70/50
Inventor 吴文果杨达云王士斌牛浩陈爱政刘源岗
Owner HUAQIAO UNIVERSITY
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