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Capacitive biosensor for identifying a microorganism or determining antibiotic susceptibility

a biosensor and capacitive technology, applied in the field of capacitive biosensors, can solve the problems of requiring an excessive amount of labor and taking a long time, and achieve the effect of effective treatmen

Inactive Publication Date: 2018-02-15
IND ACADEMIC CORP FOUND YONSEI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The biosensor and method described in this patent can quickly measure antibiotic susceptibility, which can save time in identifying microorganisms and confirming the type and concentration of antibiotic needed for treatment. This can help to effectively treat microbial infections that require quick diagnosis and treatment.

Problems solved by technology

However, these methods require a process of culturing a bacterium for several days, identifying the bacterium, and measuring turbidity, which may take a long time and requires an excessive amount of labor.

Method used

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  • Capacitive biosensor for identifying a microorganism or determining antibiotic susceptibility
  • Capacitive biosensor for identifying a microorganism or determining antibiotic susceptibility
  • Capacitive biosensor for identifying a microorganism or determining antibiotic susceptibility

Examples

Experimental program
Comparison scheme
Effect test

manufacturing example 1

nt

[0058]Cephalothin, chloramphenicol, Gentamicin, Ciprofloxacin, cefrtiaxone, and Tetracycline antibiotics were purchased from Sigma Aldrich (US), and various concentrations were prepared. Chloramphenicol was dissolved in ethanol, Ciprofloxacin was dissolved in DMSO, gentamycin and cephalothin were dissolved in distilled water, and Sephritaxone and Tetracycline were dissolved in ethanol.

[0059]The strains used in the experiment were E. coli, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecalis, as well as Ampicillin-resistant E. coli and Tetracycline-resistant E. coli.

example 2

Manufacturing Manufacturing of Capacitive Biosensor

[0060]In order to manufacture a capacitive biosensor for detecting a microorganism, a sensor (Comparative Example 1) in which electrodes are formed on a glass substrate, a sensor (Comparative Example 2) which a glass electrode formatted and a aptamer-treated sensor, and a sensor (Example 1) treated with an aptamer to AAO were manufactured.

[0061]Particularly, the porous nanostructured plate (for example, AAO plate) was formed to have a thickness of Ti / Au / Al (100 / 20 / 1000 nm) on a 4-inch Si substrate with a 1 nm thick SiO2 layer grown thereon and a thickness of Ti / Au / Al (100 / 20 / 1000 nm).

[0062]Then, the porous nanostructured plate was immersed in oxalic acid while maintaining the temperature at 15° C. using a water bath and a chiller at a concentration of 0.3M of oxalic acid, and anodic oxidation was carried out by applying a DC current of 40V. After anodic oxidation, a widening reaction was performed to produce AAO having porous nanos...

experimental example 1

ement Performance of Capacitance Change of Sensor According to Substrate

[0066]A measurement performance of a capacitance change of a sensor was confirmed using the sensor manufactured according to manufacturing Example 2. Particularly, E. coli was treated for each of a sensor (Comparative Example 1) in which an electrode was formed on a glass substrate, a sensor (Comparative Example 2) in which a glass substrate was treated with an aptamer, and a sensor (Example 1) in which AAO was treated with an aptamer, and a change in capacitance was measured.

[0067]As shown in FIG. 5, when the capacitance is measured using the sensor of the present disclosure, the change in capacitance is increased as the bacterium binds / propagates, the change in capacitance of all three treatment groups was measured, in the case of the AAO+aptamer sensor of Example 1 as compared to the glass substrate sensor (black) of Comparative Example 1 and the glass substrate sensor (red) treated with the aptamer of Compar...

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Abstract

An apparatus for inspecting an antibiotic and a method for determining antibiotic sensitivity using the same is provided. The antibiotic susceptibility inspection time which has conventionally taken longer than 24 hours is shortened to about 2 hours or less, the efficacy of the target substance is monitored in real time, the identification of the microorganism, the kind of the antibiotic capable of treating the microorganism, and the minimum dosage thereof are quickly confirmed. Microbial infections requiring prompt diagnosis and treatment can be effectively treated.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application claims priority to and the benefit of Korean Patent Application No. 10-2016-0103015, filed on Aug. 12, 2016, the disclosure of which is incorporated herein by reference in its entirety.BACKGROUND1. Field of the Disclosure[0002]The present disclosure relates to a capacitive biosensor, and more particularly, relates to a biosensor able to determine antibiotic susceptibility of a microorganism or identify a microorganism according to the result of determining a level of capacitance, changed by microorganisms, in real time.2. Discussion of Related Art[0003]An inspection to detect antibiotics that can inhibit bacterial growth is known as an antibiotic susceptibility inspection. An antibiotic susceptibility inspection is a direct and important inspection allowing a type of antibiotic to counter a bacterium to be selected. In addition, when prescribing appropriate antibiotics to a patient, such an inspection may enable a customiz...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/569G01N33/543G01N33/15C12Q1/04C12Q1/18
CPCG01N33/569C12Q1/04C12Q1/18G01N33/00G01N33/54366C12Q1/00G01N33/15G01N33/5438G01N33/56911G01N2500/10G01N27/226G01N27/227G01N27/22
Inventor YOO, KYUNG HWAHAN, NAL AEKIM, BONG JUNLEE, SUN MILIM, KOOK JINOH, JESEUNG
Owner IND ACADEMIC CORP FOUND YONSEI UNIV
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