130 results about "Cfu - colony-forming unit" patented technology

A probiotic food item containing a beneficial amount of dry active probiotic cultures is provided. The food item also contains a substantial continuous fat-based coating with an effectively low water activity level. The food item is packaged in a substantially moisture impermeable package marked with a use by or sell by date, so as to ensure a desired minimal amount of probiotic colony forming units (CFUs) on the use by or sell by date. Methods for manufacturing the probiotic food product are also provided.

The invention discloses a novel compound microorganism living bacteria agent. The agent comprises probiotics such as pediococcus, saccharomyces, bacillus and the like, wherein the total bacterial quantity is above 107 colony-forming units (cfu)/gram. The invention further discloses a method for preparing the novel compound microorganism living bacteria agent and applications of the novel compound microorganism living bacteria agent in preparation of organic sewage purification additives, deodorization fly dispelling agents, degreasing agents, pipeline blockage removing agents, feed additives and microorganism disease prevention agents. The novel compound microorganism living bacteria agent has the comprehensive effects of high removal rates to ammonia nitrogen, total nitrogen and total phosphorus, small sludge residues, energy saving, environmental friendliness and the like and the characteristics of convenience in use, safety and quickness in function, no secondary pollution, benefits to ecological protection, low treatment cost, good comprehensive profit and the like.

This invention provides a method of detecting pathogens comprising the steps of: (a) contacting a sufficient amount of biofunctional magnetic nanoparticles with an appropriate sample for an appropriate period of time to permit the formation of complexes between the pathogens in the sample and the nanoparticles; (b) using a magnetic field to aggregate said complexes; and (c) detecting said complexes. The method may further comprise the additional step of removing said complexes. The biofunctional magnetic nanoparticles are preferably a conjugate of vancomycin and FePt. The pathogens may be bacteria or viruses, and the sample may be a solid, liquid, or gas. Detection may involve conventional fluorescence assay, enzyme-linked immunosorbent assay (ELISA), optical microscope, electron microscope, or a combination thereof. The sensitivity of detection for the method is at least as low as 10 colony forming units (cfu) of the pathogens in one milliliter of solution within one hour.

The invention applies to provide a microbial deodorant which is a microbial colony composed of lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and rhodopseudomonas palustris, wherein counted by 108CFU (colony-forming unit)/ml as a unit, the total viable count is not less than 10, the lactobacillus plantarum count is not less than 3, the saccharomyces cerevisiae count is not less than 3, the rhodopseudomonas palustris count is not less than 2 and the bacillus subtilis count is not less than 2. The microbial deodorant applied by the invention has stronger zymolysis ability under both aerobic and anaerobic conditions, and the beneficial bacteria mutually play a role in inhibiting the growth of putrefactive bacteria and interrupting the putrefactive process, secrete organic acid while reproducing quickly, and generate antioxidation substances, thus converting odorous substances such as NH3, H2S, indoles and the like into non-odorous substances.

A silk protein membrane is described which is loaded with an antimicrobial compound and has a substantially non-granular ultrastructure which is (i) substantially devoid of micellar silk fibroin substructures and (ii) substantially devoid of pores when analysed by scanning electron microscopy at 0.2 μm resolution. The antimicrobial compound comprises, in one aspect of the invention, a host defense peptide. The silk protein membrane of the invention can be used in a method for the treatment of wounds and allows the wound dressing to be kept in place after removal of that wound dressing from a wound the wound has less than 10⁵ colony forming units per gram. A method for manufacturing a wound dressing is also disclosed which comprises transferring a cast precursor material and optionally a host defense peptide, into a solid support and then drying the precursor material on the solid support to form a silk protein membrane for use as the wound dressing.

A method is provided for forming aseptic or substantially aseptic concentrated dairy liquid, such as dairy milk, without significant heat treatment. In one form, the method first concentrates a starting dairy milk to about 2× to about 7× concentration using an ultrafiltration membrane to form a dairy concentrate. Thereafter, the dairy concentrate is filtered using a microfiltration membrane to provide the aseptic or substantially aseptic concentrated dairy milk. The resultant concentrated dairy milk has less than about 0.5 percent total bacteria and less than about 5 colony forming units of spore forming bacteria per gram. The substantially aseptic concentrated dairy milk is not subjected to significant heat treatment during processing.

The invention relates to biofertilizer for improving saline and alkaline lands. The biofertilizer comprises the following components in parts by weight: 200 to 250 parts of fermented mushroom residue, 150 to 200 parts of ammonium sulfate, 50 to 100 parts of superphosphate, 50 to 100 parts of complex improver, 15 to 21 parts of EDTA (ethylene diamine tetraacetic acid)-metallo-chelate, 0.5 to 1 parts of ammonium molybdate and 5 to 10 parts of borax, and also comprises 20 to 30 parts of compound micro-organism, so that the living bacteria count of the biofertilizer is not less than 5.0 * 108CFU (colony-forming unit) per gram. The invention also relates to a preparation method for the biofertilizer and the application of the biofertilizer in crop planting. The biofertilizer for improving the saline and alkaline lands, disclosed by the invention, has important functions of improving the soil conditions of the saline and alkaline lands, improving the soil fertility, increasing crop yield and preventing the soil from secondary salinization.

The invention discloses a method for recovering cadmium polluted soil by combining a compound microorganism bacterium agent with houttuynia cordata. The compound microorganism bacterium agent comprises the following components by count: 1*10<9>-9*10<9>CFU (colony forming unit)/m<3> pseudomonas fluorescens, 1*10<8>-6*10<8>CFU/m<3> actinomyces ruminicola, and 1*10<9>-9*10<9>CFU/m<3> glomus mosseae. The method for recovering the soil comprises the following steps that the houttuynia cordata is planted in cadmium soil and the compound microorganism bacterium agent is inoculated into the soil, or the houttuynia cordata is soaked in the compound microorganism bacterium agent and then planted into the cadmium soil; when branches and leaves of the houttuynia cordata are luxuriant, the branches and the leaves of the houttuynia cordata are harvested; and cadmium in the soil can be removed. The compound microorganism bacterium agent can better improve the tolerance of the houttuynia cordata, promote growth of a plant in the high-cadmium-content soil, improve the absorbility of the plant to cadmium, and enhance a soil heavy metal removal effect. The method for recovering the soil is a safe, environment-friendly, high-efficiency, economical and practical plant-microorganism combined method for recovering the heavy metal polluted soil, and has a significant application value.

The invention discloses a production method of a microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes. The production method is characterized by comprising the following steps: carrying out symbiotic assemblage screening on lactic acid bacteria and saccharomycetes separated from an Inner Mongolia dairy product kumiss to select a set of enterococcus faecium HE5 and brewer yeast J-4 with mutual promotion, wherein the viable count of the lactic acid bacteria reaches 2.88*10<9>CFU (Colony-Forming Unit)/mL when enterococcus faecium HE5 and brewer yeast J-4 screened are cultivated on an optimized skimmed milk culture medium, the viable count of the saccharomycetes reaches 3.3*10<8>CFU/mL, and double bacteria culture is better to promote the growing effect of lactic acid bacteria; and freezing and drying the cultivated and harvested double bacteria to obtain the microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes. The microbial preparation is good in symbiotic effect and strong in acid-producing capacity.

The invention discloses a Bu lactobacillus preparation that contains 1*109-1*1011 Bu lactobacillus colony forming unit. It also discloses the manufacture method and the usage. It could used to improve the quality of corn silage.

The invention relates to the field of microorganism application, in particular to Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparations and application of the Bacillus licheniformis. The invention provides Bacillus licheniformis CRVAB001 for producing lactic acid at high yield after fermentation in the collection number of CGMCC No.4850. The inventionalso provides a microecological liquid preparation comprising the Bacillus licheniformis, a preparation method for the microecological liquid preparation, microecological powder comprising the Bacillus licheniformis, and a preparation method for the microecological powder. The Bacillus licheniformis can be efficiently and quickly fermented to produce the lactic acid, and has great industrial application prospect. The viable content of the liquid preparation is more than 1*10<10> colony-forming units (CFU)/mL, the viable content of the powder is more than 1*10<11>CFU/g, and the effectiveness of the Bacillus licheniformis preparations is fully guaranteed.

The invention provides a probiotic composition and a preparation method and application thereof. The probiotic composition comprises raw materials of lactobacillus reuteri, lactobacillus acidophilus and bifidobacterium animalis, wherein the weight ratio of the lactobacillus reuteri to the lactobacillus acidophilus to the bifidobacterium animalis is (1.5-2.5):(0.5-1.5):(0.5-1.5). The colony forming units of the lactobacillus reuteri, the lactobacillus acidophilus or the bifidobacterium animalis in unit weight are the same. The lactobacillus reuteri is preserved in China Center for Type Culture Collection with the preservation number of 209138, and the lactobacillus acidophilus and/or the bifidobacterium animalis preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC 15210 are/is bifidobacterium animalis BB-115. The probiotic composition can relieve the symptom of slow transit constipation under the condition of low dosage, and can remarkably and effectively regulate gastrointestinal regulatory peptide indexes related to constipation, such as P substance (SP), gastrin (Gas) and vasoactive peptide (VIP).

The invention discloses a polycyclic aromatic hydrocarbon antibacterial agent of a composite carrier. The polycyclic aromatic hydrocarbon antibacterial agent comprises a Mycobacterium vanbaalenii. PYR-1 bacterium suspension and a carrier, wherein the volume/mass ratio of the bacterium suspension to the carrier is (20-50)ml: 15g; (5.0-6.0)*10<8>CFU (Colony-Forming Unit) of the Mycobacterium vanbaalenii. PYR-1 thallus is contained in the bacterium suspension per ml; the carrier comprises black charcoal and humus in the mass ratio of (1.8-2.2):1. The invention also discloses a preparation method of the polycyclic aromatic hydrocarbon antibacterial agent of the composite carrier. The polycyclic aromatic hydrocarbon antibacterial agent is capable of effectively reducing the content of the polycyclic aromatic hydrocarbon in the polluted environment.

A waste treatment method includes the concentration of selected strains of bacteria in a selected medium in the presence of nutrients and water, under aerobic conditions. This concentrated batch is thereafter discharged for downstream applications in wastewater remediation. The present invention employs a cultivation chamber having inlet ports and a circular vent port that allows for adequate air introduction and heat release. Aeration is achieved by recirculation of the fluid medium from the top of the apparatus through a pipe that runs the length of the inner wall and is specially configured at the top to minimize cell damage. Fluid can be routed tangentially in both the clockwise and counterclockwise directions within the chamber. The conical bottom also has an orifice allowing for recirculation of the fluid medium tangentially to the sidewalls. Upon completion of the batch cultivation, the medium and bacteria are discharged for downstream applications in wastewater remediation of paper mill, chemical plant, oil refinery, and other industrial effluents. The aeration and circulation of the fluids is designed to limit cells shearing and damage yet achieve critical cell mass (e.g. between 10⁷ and 10¹⁰ cfu (colony forming units) per milliliter (ml)) and dissolved oxygen uptake rates (e.g. between about 50 and 1500) during cultivation.

The invention discloses a formula and a preparation method of a yeast liquid biocontrol bactericide. The formula comprises yeasts, sucrose, trehalose, galactose, ascorbic acid and a 0.05 mol/L phosphate buffer solution. The method comprises the following steps: activating a yeast strain 4-4A by an NYDB (nutrient yeast dextrose broth) liquid culture medium and inoculating two rings into 50 ml of a seed culture medium; putting a cultured yeast fermentation solution into 50 ml of a centrifugal pipe for conducting a centrifuging process at a speed of 4000 r/min for 15 minutes, dumping supernatant liquor, washing by aseptic water for two times, and adding obtained sediments into the 0.05 mol/L phosphate buffer solution to prepare a bacterial suspension solution; selecting the sucrose, the galactose and the trehalose as protecting agents for preparing a solution by the phosphate buffer solution; adding 21.6 ml of the solution into 2.4 ml of a yeast mother solution with 109 CFU/ml (colony forming unit/ml) of the yeasts, fully and uniformly mixing, and sub-packaging a mixture into 1.5 ml of the centrifugal pipe; measuring the number of living yeasts in a yeast preparation. According to the yeast liquid biocontrol bactericide, the occurrence rate of anthracnose after mangoes are harvested is effectively reduced, the yeast liquid biocontrol bactericide is applied for 4-6 times from an early flowering period to a harvest period to prevent and control mango anthracnose, the morbidity and the severity are reduced by 64%-67% and 89%-90% respectively, and the disease prevention and control effect is improved.

A process for producing a meat slurry is described. The process includes: (i) obtaining an animal source of protein; (ii) reducing piece size of the animal source of protein to produce a smaller-piece-sized animal source of protein; (iii) introducing one or more types of lactic acid producing bacteria and at least one carbohydrate energy source to the smaller-piece-sized animal source of protein to produce a meat mixture; (iv) adding water to the meat mixture to produce an activated meat mixture; (v) incubating the activated meat mixture to produce a meat slurry; and wherein the activated meat mixture has a pH value that is greater than about 5.0, and wherein prior to incubating, the lactic acid producing bacteria is present on the meat mixture in an amount that is at least about 1×107 colony forming units (“CFU”) of lactic acid producing bacteria per gram of said meat mixture, and wherein after incubating, the lactic acid producing bacteria produces lactic acid in an amount that is sufficient to maintain a pH of the meat slurry at a value that is no greater than about 4.7.

The present invention provides a method for coating seeds with biological materials such as bacteria, fungi (e.g., yeasts and molds), parasites, recombinant vectors, and viruses. The method comprises (a) applying a moistening liquid to seeds to moisten seeds, wherein the moistening liquid comprises a moistening polymer, and (b) coating the moistened seeds with an effective amount of a dry composition, wherein the dry composition comprises biological materials, one or more disaccharides, one or more oligosaccharides, one or more polysaccharides, one or more carboxylic acid salts, and one or more hydrolyzed proteins. The coated seeds may have an initial water activity (Aw) below 0.4, and the microorganisms on the coated seeds have initial viability of at least 5 logs of colony forming units per gram of seeds (CFU/g seed). Also provided are coated seeds.

The invention discloses a straw decomposition agent. An active ingredient of the straw decomposition agent is prepared from actinomycetes, trichoderma harzianum, saccharomyces and bacillus subtilis which are in the bacterial flora formation unit number ratio of (0.5 to 2): (1 to 2): (0.8 to 1.2): 1. According to the straw decomposition agent disclosed by the invention, all bacterial florae can harmoniously coexist and mutually promote so as to achieve the effect of having complementary advantages, and lignin, cellulose, hemicellulose and the like in crop straws can be effectively decomposed; the degradation time is short, the decomposition effect is stable, diseases and insect pests are eliminated effectively, and the straw decomposition agent has a decomposition effect at normal temperatures, so that the rapid treatment of organic materials and final resource-converting utilization are achieved; treated organic solid wastes are transformed thoroughly, embody good properties of improving soil, promoting growth and inhibiting soil-borne diseases and meanwhile are environmentally friendly and sanitary.

The invention relates to a thorough decomposition agent for locally returning straw to fields, and a preparation method of the thorough decomposition agent, and belongs to the technical field of thorough decomposition agents for straw. The thorough decomposition agent disclosed by the invention has the characteristics of being high in decomposition and disintegration efficiency and short in degradation time, and particularly has favorable disintegration effects at normal temperature and even low temperature. The thorough decomposition agent for locally returning straw to fields, disclosed by the invention, is prepared through the steps of performing fermentation with bacillus amyloliquefaciens, aspergillus niger, trichoderma longibrachiatum and saccharomyces cerevisiae respectively to obtain fermentation liquid, and mixing the fermentation liquid, wherein the ratio of the unit number of colonies of bacillus amyloliquefaciens agent fermentation liquid to the unit number of colonies of aspergillus niger agent fermentation liquid to the unit number of colonies of trichoderma longibrachiatum agent fermentation liquid to the unit number of colonies of saccharomyces cerevisiae fermentation liquid is 2:3:3:1. The thorough decomposition agent for locally returning straw to fields, obtained by the preparation method disclosed by the invention can promote rapid thorough decomposition ofthe straw and enable the thoroughly decomposed straw to return to the fields, can supplement soil organic matter, can sufficiently release and utilize nitrogen, phosphorus and potassium nutrients in the straw, and besides, can improve soil microorganism environment.

Methods are provided for obtaining expanded human cord blood cells expressing CD34. The methods involve seeding a sufficient amount of cord blood cells with a sufficient amount of menstrual cells under co-culture conditions suitable to promote expansion of the cord blood cells, and co-culturing the cord blood cells with the menstrual cells under culture conditions that support at least two or more population doublings of the cord blood cells. Methods are also provided for growing expanded human cord blood cells to give rise to any one of colony forming units, colony forming unit granulocyte macrophages (CFU-GM), burst forming unit erythroids (BFU-E), and colony forming unit granulocyte erythrocyte macrophage megakaryocyte (CFU-GEMM) blood lineage precursor cells. The expanded cells may express CD34, SSEA-4, and HLA-II. Compositions of the expanded cells are also provided.

The invention discloses a microbial powder, an infant yoghourt and a preparation method thereof. The microbial powder comprises a compound microbial powder of streptococcus thermophilus STI-13 and streptococcus thermophilus Body-3 at a ratio of 1:1 to 1:4 by colony forming unit quantity. The infant yoghourt obtained by fermentation by using the microbial powder is free of D-lactic acid, and the protein component, the fat structure component and the fatty acid component are all similar to those of breast milk, therefore the infant yoghourt can be favorably digested and assimilated by infants.