Straw in-situ decomposition microbial agent and application thereof

A technology of bacteria agent and straw, which is applied in the direction of application, bacteria, fixed on or in the inorganic carrier, can solve the problems of difficult to ensure the moisture of microbial proliferation, unclear composition of straw, etc., and achieve rapid decomposition, low cost, and wide The effect of applying the foreground

Inactive Publication Date: 2014-03-19
MOUNT EMEI GREEN LAND ECOLOGICAL AGRI DEV LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many kinds of decomposing bacteria agents for straw returning to the field that have been reported or applied for patents, but there are still some problems as follows: (1) For the in-situ rapid decomposition of straw, the object of priority rapid decomposition (straw components) is not clear
However, when the actual straw is returned to the field, the surface soil in the aerobic environment in the field is difficult to ensure the moisture for microbial proliferation; in addition, the 2-10 cm layer below the surface where the returned straw is concentrated is in the micro-aerobic layer transitioning from aerobic to anaerobic, and Not an aerobic environment for fungal growth
Therefore, emphasizing the combination of fungi or aerobic decomposing bacteria using pure aerobic bacteria cannot achieve the best in-situ decomposition effect when straw is returned to the field

Method used

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  • Straw in-situ decomposition microbial agent and application thereof
  • Straw in-situ decomposition microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the preparation of straw decomposing bacterial agent in situ

[0043] 1. Experimental materials

[0044] 1. The strains used for the preparation of bacterial agents

[0045] Clostridium xylanolyticum (Clostridium xylanolyticum) DSM No.6555T: This strain is preserved in the German Culture Collection of Microorganisms (DSMZ), and the preservation number is DSM No.6555T;

[0046] Cellulolytic Xylanomonas (Xylanimonas cellulosilytica) DSM No.15894: This strain is preserved in the German Microorganism Culture Collection (DSMZ), and the preservation number is DSM No.15894;

[0047] Pseudomonas (Pseudomonas sp.) ACCC No.11691: This strain is preserved in the China Agricultural Microorganism Culture Collection Center (ACCC), and the preservation number is ACCC No.11691;

[0048] Bacillus subtilis (Bacillus subtilis) ACCC No.03189: This strain is preserved in the China Agricultural Microorganism Culture Collection Center (ACCC), and the preservation number is ACC...

Embodiment 2

[0062] Example 2, Analysis of the decomposing effect of straw in-situ decomposing bacteria agent on straw in culture medium

[0063] 1. Analysis of the decomposing effect of straw in-situ decomposing bacterial agent I prepared in Example 1 on corn stalks in the culture medium

[0064] Inoculate the straw in situ decomposing bacterial agent I prepared in Example 1 into a 100mL Erlenmeyer flask filled with 50mL medium with 10g / L corn stalk as a carbon source, and culture it statically at 30°C with an initial pH of 8.0 After 15 days, the removal rate of cellulose and hemicellulose in corn stalks was measured after the cultivation.

[0065] Among them, the specific formula of the medium with 10g / L corn stalk as carbon source is as follows: peptone 5g / L, yeast extract 1g / L, NaCl 5g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O 0.35g / L, CaCO 3 3g / L, corn straw 10g / L, pH=7.2±0.1. The particle size of corn stalks is 2-10 cm.

[0066] The method for determining the removal rate of cellulose...

Embodiment 3

[0079] Example 3. Straw in-situ decomposing bacteria agent I is applied to the direct return of corn stalks to the field

[0080] Experimental location: Shangzhuang Experimental Station of China Agricultural University, Haidian District, Beijing.

[0081] Experimental materials: corn stalks, straw in situ decomposing bacterial agent I prepared in Example 1.

[0082] Experimental design: set up two experimental points, each experimental point has two treatments, each treatment takes an area of ​​not less than 0.5 mu, and the treatments are randomly arranged to ensure that the experimental conditions are basically the same.

[0083] Treatment 1: Without adding fungicides, the corn stalks were directly returned to the field.

[0084] Treatment 2: Using the in-situ straw decomposing bacterial agent I prepared in Example 1, the corn straw was directly returned to the field.

[0085] Application method: After crushing corn stalks (with a particle size of 2-5cm), sprinkle them even...

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Abstract

The invention discloses a straw in-situ decomposition microbial agent. Active ingredients of the microbial agent are formed by mixing clostridium xylanolyticum, xylan monad cellulolyticus, pseudomonas and bacillus subtilis in a CFU (Colony Forming Unit) ratio of (25-35): (25-30):(20-30):(15-20). Experiments prove that the microbial agent provided by the invention has degradation rate for straws, which can reach 70%-80% in a culture medium, and can reach over 50% when the straws are returned to the field for 20 days. The microbial agent disclosed by the invention is low in cost and simple to prepare. The microbial agent disclosed by the invention breaks through the limit that natural cellulose cannot be efficiently decomposed by purifying bacteria and clastic enzymes thereof, provides a key technology for straw in-situ rapid decomposition, and has a wide application prospect in the field of returning straws to the field and decomposing a lignocelluloses material.

Description

technical field [0001] The invention relates to a straw decomposing bacterial agent and its application, in particular to a straw in-situ decomposing bacterial agent which preferentially decomposes hemicellulose and its application. Background technique [0002] Straw returning is a traditional agricultural measure, which is of great significance to increase soil organic matter content, maintain soil fertility, and achieve sustainable agricultural development. In recent years, with the development of agricultural mechanization and microbial technology (microbial preparations), the direct return of straw to the field has generally received attention and has been widely promoted and applied nationwide. To a certain extent, the waste phenomenon of straw burning has been corrected, and the damage to the atmospheric environment and ecological environment has been reduced. At the same time, soil fertility has been fertilized, and the organic matter content of the soil has been eff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/12C05F17/00C05F11/08C12R1/145C12R1/38C12R1/125C12R1/01
CPCY02W30/40
Inventor 崔宗均袁旭峰袁维强王小奎
Owner MOUNT EMEI GREEN LAND ECOLOGICAL AGRI DEV LIMITED
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