Formula and preparation method of yeast liquid biocontrol bactericide

Abstract

The invention discloses a formula and a preparation method of a yeast liquid biocontrol bactericide. The formula comprises yeasts, sucrose, trehalose, galactose, ascorbic acid and a 0.05 mol / L phosphate buffer solution. The method comprises the following steps: activating a yeast strain 4-4A by an NYDB (nutrient yeast dextrose broth) liquid culture medium and inoculating two rings into 50 ml of a seed culture medium; putting a cultured yeast fermentation solution into 50 ml of a centrifugal pipe for conducting a centrifuging process at a speed of 4000 r / min for 15 minutes, dumping supernatant liquor, washing by aseptic water for two times, and adding obtained sediments into the 0.05 mol / L phosphate buffer solution to prepare a bacterial suspension solution; selecting the sucrose, the galactose and the trehalose as protecting agents for preparing a solution by the phosphate buffer solution; adding 21.6 ml of the solution into 2.4 ml of a yeast mother solution with 109 CFU / ml (colony forming unit / ml) of the yeasts, fully and uniformly mixing, and sub-packaging a mixture into 1.5 ml of the centrifugal pipe; measuring the number of living yeasts in a yeast preparation. According to the yeast liquid biocontrol bactericide, the occurrence rate of anthracnose after mangoes are harvested is effectively reduced, the yeast liquid biocontrol bactericide is applied for 4-6 times from an early flowering period to a harvest period to prevent and control mango anthracnose, the morbidity and the severity are reduced by 64%-67% and 89%-90% respectively, and the disease prevention and control effect is improved.

Description

technical field [0001] The invention belongs to the technical field of biocontrol agent preparation, and in particular relates to a formula and a preparation method of yeast liquid biocontrol agent. Background technique [0002] At present, anthracnose harms mango before and after harvest, causing great yield and quality loss. It is one of the important diseases in mango production. Its pathogenic bacteria is mainly Colletotrichum gloeosporioides (Penz) Penz. & Sacc. ). For a long time, the field control of this disease has mainly relied on chemical fungicides. With the long-term and large-scale use of benzimidazole chemical agents, G. anthracnose has developed resistance to these fungicides, resulting in a decline in the control effect. The latent infection of pathogenic bacteria will cause a lot of losses in the postharvest circulation process of mangoes. The prevention and treatment of postharvest anthracnose also requires chemical agents. As people pay more and more att...

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Problems solved by technology

[0003] The purpose of the embodiments of the present invention is to provide a formula and preparation method of a yeast liquid biocontrol agent, aiming at solving the problem that the existing chemical fungicides used in mango anthracnose control have improved the drug resistance of the bacteria, which is not conducive to environmental protection and environmental protection. Food Safety Issues

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