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Polycyclic aromatic hydrocarbon antibacterial agent of composite carrier and preparation method of polycyclic aromatic hydrocarbon antibacterial agent

A technology of polycyclic aromatic hydrocarbons and composite carriers, which is applied to the restoration of contaminated soil fixed on or in inorganic carriers, which can solve the problems of insufficient nutrition in the matrix and failure to achieve the expected repair effect, and achieve small environmental heterogeneity , Improving the survival rate and tolerance of microorganisms, and the effect of a wide range of sources

Inactive Publication Date: 2012-03-28
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

In fact, PAH-degrading bacteria will be affected by other factors in the environmental medium such as the adaptability of the new environment (differences in pH, temperature, humidity, etc.), insufficient nutrients in the substrate, and competition with other microorganisms in the medium during the actual remediation application process. Did not play the expected repair effect

Method used

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  • Polycyclic aromatic hydrocarbon antibacterial agent of composite carrier and preparation method of polycyclic aromatic hydrocarbon antibacterial agent
  • Polycyclic aromatic hydrocarbon antibacterial agent of composite carrier and preparation method of polycyclic aromatic hydrocarbon antibacterial agent

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Embodiment 1

[0036] Embodiment 1, the preparation method of the polycyclic aromatic hydrocarbon degrading bacterial agent of a kind of composite carrier, carry out following steps successively:

[0037] 1), the preparation of bacterial suspension:

[0038]A single colony of Mycobacterium vanbaalenii.PYR-1 was inoculated into an Erlenmeyer flask filled with LB medium, and cultured at 30°C, 120 rpm, in a dark water bath for 24 hours with shaking. Bacterial suspension. Adjust the above bacterial suspension to about OD600=0.6, that is, each ml of Mycobacterium vanbaalenii.PYR-1 bacterial suspension contains 5.3*10 8 CFU of Mycobacterium vanbaalenii.PYR-1 cells.

[0039] The preparation method of LB culture medium is as follows: add yeast extract (yeast extract) 5g, peptone 10g and Nacl 5g to every liter of water, use NaOH to adjust the pH to 7.4~7.6, and then obtain through conventional autoclaving.

[0040] 2), preparation of carrier raw materials:

[0041] Black carbon (100 mesh) is adju...

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Abstract

The invention discloses a polycyclic aromatic hydrocarbon antibacterial agent of a composite carrier. The polycyclic aromatic hydrocarbon antibacterial agent comprises a Mycobacterium vanbaalenii. PYR-1 bacterium suspension and a carrier, wherein the volume / mass ratio of the bacterium suspension to the carrier is (20-50)ml: 15g; (5.0-6.0)*10<8>CFU (Colony-Forming Unit) of the Mycobacterium vanbaalenii. PYR-1 thallus is contained in the bacterium suspension per ml; the carrier comprises black charcoal and humus in the mass ratio of (1.8-2.2):1. The invention also discloses a preparation method of the polycyclic aromatic hydrocarbon antibacterial agent of the composite carrier. The polycyclic aromatic hydrocarbon antibacterial agent is capable of effectively reducing the content of the polycyclic aromatic hydrocarbon in the polluted environment.

Description

technical field [0001] The invention relates to the microbial remediation of soil polluted by polycyclic aromatic hydrocarbons, in particular to the preparation and application of a microbial agent for removing polycyclic aromatic hydrocarbons. Background technique [0002] With the acceleration of industrialization, the amount of toxic and harmful substances released into the soil environment far exceeds its self-purification ability. Among them, the pollution concentration of polycyclic aromatic hydrocarbons (PAHs), a common class of persistent organic pollutants, has increased from ug / kg The magnitude increased to the mg / kg level, and the detection rate increased from less than 20% to more than 80%. Polycyclic aromatic hydrocarbons (PAHs) refer to aromatic compounds with two or more benzene rings fused together, which are mainly caused by the incomplete combustion of organic matter at high temperature, and most of them have "three effects" effect. PAHs have a high octano...

Claims

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Application Information

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IPC IPC(8): C12N11/14B09C1/10A62D3/02A62D101/20
Inventor 徐建明何艳王娇娇徐民民马斌
Owner ZHEJIANG UNIV
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