Novel compound microorganism living bacteria agent, method for preparing same and applications

A technology of compound microorganisms and live bacteria preparations, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large odor, large amount of excess sludge, and high energy consumption

Inactive Publication Date: 2013-04-10
SHANGHAI ECO WELL BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the shortcomings of large amount of residual sludge, large odor, high energy consumption and high cost in the existing technology for treating organic sewage, the present invention provides a method that can avoid the above-mentioned defects and

Method used

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  • Novel compound microorganism living bacteria agent, method for preparing same and applications
  • Novel compound microorganism living bacteria agent, method for preparing same and applications
  • Novel compound microorganism living bacteria agent, method for preparing same and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Carry out amplified culture respectively:

[0049] ①Bacillus [Bacillus Horikoshi (CICIM B1319), Bacillus Tequila (ATCC BAA-819), Bacillus coagulans (CGMCC 1.2009), Bacillus amyloliquefaciens (CGMCC 1.1099) and Bacillus avermitilis (DSM 16317) )] were respectively activated on the slant (the slant medium is LB medium, 30°C, 48 hours), and then inoculated into the liquid medium (liquid medium formula: yeast extract 5g, peptone 10g, NaCl 10g, distilled water 1000mL, pH 7.0), incubate at 30°C for 24 hours.

[0050] ② Yeasts [Pichia pastoris (CGMCC 2.1463), Dekkera (ATCC10559)] were respectively activated on the slant (the slant medium is YPD medium, 30°C, 48 hours), and then inoculated into the medium in sequence ( Liquid medium formula: Dissolve 10g yeast extract and 20g peptone in 900ml water, sterilize under high pressure at 121°C for 20 minutes; then add 100ml of sterilized solution containing 20g glucose (115°C for 20 minutes), and incubate at 30°C for 48 hours. ...

Embodiment 2

[0055] (1) Carry out amplified culture respectively (the slant medium used for each bacteria and the method of counting viable bacteria are the same as in Example 1):

[0056] Pediococcus [Pediococcus pentosaceae (ATCC 25744), Pediococcus lactis (ATCC 12697) and Pediococcus cerevisiae (CGMCC 1.123)] were respectively activated on the slant, and then inoculated in liquid medium in turn, cultured at 35°C for 48 hours, liquid Medium formula: glucose 3%, tryptone 1.5%, beef extract 3%, peptone 2%, Tween-800.1%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH 6.5.

[0057] Yeasts [Pichia pastoris (CGMCC 2.1463) and Saccharomyces cerevisiae (CGMCC 2.769)] were respectively activated on slant surfaces, inoculated in liquid medium in turn, and cultured at 28°C for 48 hours. Liquid medium formula: glucose 1%, malt Powder 0.2%, yeast extract 0.2%, sodium acetate 0.5%, pH 7.

[0058] Bacillus [Bacillus amyloliquefaciens (CGMCC 1.1099), ...

Embodiment 3

[0062] (1) Carry out amplified culture respectively (the slant medium used for each bacteria and the method of counting viable bacteria are the same as in Example 1):

[0063] Pediococcus [Pediococcus pentosaceae (ATCC 25744), Pediococcus cerevisiae (CGMCC 1.123)] were respectively activated on the slant, inoculated in the liquid medium in turn, and cultured at 33°C for 48 hours. The liquid medium formula: glucose 3%, Tryptone 1.5%, beef extract 3%, peptone 2%, Tween-800.1%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH 6.5.

[0064] Yeasts [Pichia anomaly (CGMCC 11061), Dekkera (ATCC 10559) and Debariae (CGMCC 2.1462)] were respectively activated on the slant, inoculated in liquid medium in sequence, and cultured at 27°C for 48 hours. Liquid medium formula: glucose 1%, malt powder 0.2%, yeast extract 0.2%, sodium acetate 0.5%, pH 7.0.

[0065] Bacillus [Bacillus subtilis (CGMCC 1.1414), Bacillus coagulans (CGMCC1.2009), Bac...

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Abstract

The invention discloses a novel compound microorganism living bacteria agent. The agent comprises probiotics such as pediococcus, saccharomyces, bacillus and the like, wherein the total bacterial quantity is above 107 colony-forming units (cfu)/gram. The invention further discloses a method for preparing the novel compound microorganism living bacteria agent and applications of the novel compound microorganism living bacteria agent in preparation of organic sewage purification additives, deodorization fly dispelling agents, degreasing agents, pipeline blockage removing agents, feed additives and microorganism disease prevention agents. The novel compound microorganism living bacteria agent has the comprehensive effects of high removal rates to ammonia nitrogen, total nitrogen and total phosphorus, small sludge residues, energy saving, environmental friendliness and the like and the characteristics of convenience in use, safety and quickness in function, no secondary pollution, benefits to ecological protection, low treatment cost, good comprehensive profit and the like.

Description

technical field [0001] The invention relates to a novel composite microbial living bacteria preparation for treating organic sewage, a preparation method and application thereof. Background technique [0002] At present, the following methods are usually used in China to treat organic sewage such as urban domestic sewage, food industry wastewater, and animal manure sewage: when the COD is greater than 1000mg / L, an anaerobic and then aerobic biological treatment process is adopted; when the COD is less than 1000mg / L, the anoxic-aerobic biological treatment process (activated sludge method, biofilm method) is adopted. After the treatment by the above method, the general COD can reach the standard, but the treatment effect of ammonia nitrogen, total nitrogen and total phosphorus is poor. In addition, activated sludge has a large amount of residual sludge, a strong foul smell during sludge treatment, a large amount of aerobic aeration, and high operating costs. Since the 1990...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/16C12N1/18C12N1/20C02F3/34C11D3/00A23K1/16A61K36/064A61K35/74A61P31/00C12R1/01C12R1/07C12R1/125C12R1/10C12R1/11C12R1/725C12R1/865C12R1/84C12R1/645A61K35/741A61K35/742
Inventor 曾伟明贺启环王世民王韵周懋安
Owner SHANGHAI ECO WELL BIOSCI
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